Current Annotation for L35496_AD370_D1 23/11/17

namestartcoordendcoord**deintronstart1directionstatusgnecgpcctype1kognumkogglimmerdompatauth10/10/03 00:00:00More Comments 
                    10/10/03 00:00:00  
LUAcid_AD370_D1_011595372  ABC efflux pump, inner membrane subunit 1RADDED   - best match to Acidobacteria bacterium Ellin345 (35% identity) - matches in Bacteria, not A and not E - also CO0318 IQ lipid transport and metabolism - no matches on other clones - length good start GTG PROTEINCOG0577 ABC-type antimicrobial peptide transport system, permease componentV Defense mechanismsGood 82.227.62.18014/09/07 14:00:14  
LUAcid_AD370_D1_0222481751  hypothetical protein 1RADDED   - best and single in database match to Acid_6477 solibacter (50% identity) --> unique in subgroup of Acidobacteria - no matches to other acidobacterial clones -PROTEIN  Goodunique82.227.62.18014/09/07 14:04:14  
LUAcid_AD370_D1_0324464167  gamma-glutamyltransferase 2DADDED   - best match Solibacter Acid_2807 (54% identity) - present on other clones:KM3_187_G1_308; AD106_G4_404, KM3_47_B8_164; AD55_D2_062 - in all kind of BacteriaPROTEINCOG0405 Gamma-glutamyltransferaseE Amino acid transport and metabolismGoodB82.227.62.18014/09/07 14:23:45  
LUAcid_AD370_D1_0452884245  hypothetical protein 1RADDED   - low similarity to putative type IV pilin secretion protein [Haemophilus influenzae PittGG] only 27% identity and different lengthPROTEIN  Goodunique82.227.62.18014/09/07 14:26:23  
LUAcid_AD370_D1_051135313011  cotransporter/symporter protein 50DADDED   - best match to Bacteroides thetaiotaomicron VPI-5482 (52% identity) - conserved in CFB group of Bacteria and Eukaryotes - low matches in both Ellin345 and Ellin6706 - low match to KM3_18_F8_238 - start changed to 50aa, ORF too longPROTEINCOG4146 Predicted symporterR General function prediction onlyGoodBE82.227.62.18014/09/07 15:35:22  
LUAcid_AD370_D1_061312513664  protein of unknown function DUF177 1DADDED   - best match to Solibacter usitatus Ellin6076 (38% id) - one match in each acido genome - not in other clones - length OKPROTEINCOG1399 Predicted metal-binding, possibly nucleic acid-binding proteinR General function prediction onlyGoodB82.227.62.18014/09/07 17:49:05- envirnoment conserved in Ellin345 and Ellin6706 and cloneAD370_D1_152? - and in addition ribosomal protein L32 - DUF177, Rib L12, fatty acid synthase in syntheny in Acidobacteria and also in other Bacteria: Geobacter, Pelobacter.... 
LUAcid_AD370_D1_071370313882  ribosomal protein L32 1DADDED   - best match Acid345_4144 (60% id) - very conserved in B - Pfam1783PROTEIN  -B82.227.62.18014/09/07 17:57:22- envirnoment conserved in Ellin345 and Ellin6706 and cloneAD370_D1_152? - and in addition ribosomal protein L32 - DUF177, Rib L12, fatty acid synthase in syntheny in Acidobacteria and also in other Bacteria: Geobacter, Pelobacter.... 
LUAcid_AD370_D1_081395314975  fatty acid/phospholipid synthesis protein PlsX 1DADDED   - present in both acido genomes - best match Solibacter (64% identity) - Acid_6758 and Acid345_4145 - also COG0365PROTEINCOG0416 Fatty acid/phospholipid biosynthesis enzymeI Lipid transport and metabolismGoodB82.227.62.18014/09/07 18:02:10- environment conserved in Ellin345 and Ellin6706 and cloneAD370_D1_152? - and in addition ribosomal protein L32 - DUF177, Rib L12, fatty acid synthase in syntheny in Acidobacteria and also in other Bacteria: Geobacter, Pelobacter.... 
LUAcid_AD370_D1_091577014985  acyl-(acyl-carrier-protein)--UDP-N-acetylglucosamine O-acyltransferase 1RADDED   - best match Solibacter usitatus Ellin6076 (42% Id) - also COG1044 - IPR001451: bacterial transferase domain ”hexapep”PROTEINCOG1043 Acyl-[acyl carrier protein]--UDP-N-acetylglucosamine O-acyltransferaseM Cell wall/membrane/envelope biogenesisGoodB82.227.62.18014/09/07 18:09:31- environment not conserved - not in other acido clones 
LUAcid_AD370_D1_101678015863  conserved hypothetical protein 1RADDED   - best match to Bacillus (34%) - DNA repair photolyase annotation and COG? - DUF85 and Radical_SAM domain - also COG0440 amino acid transport and metabolism? - one low match in Methanogen (Archaea) - length OKPROTEINCOG1533 DNA repair photolyaseL Replication, recombination and repairGoodB82.227.62.18014/09/07 18:26:02- one copy in each acidobacterial genome 
LUAcid_AD370_D1_121782017518  conserved hypothetical 1RADDED   - Antibiotic biosynthesis monooxygenase ? - best match Geobacter bemidjiensis Bem (51% id) - length OK - good - low matches in A and EPROTEINCOG1359 Uncharacterized conserved proteinS Function unknownGoodB82.227.62.18014/09/07 18:36:23- no match in clones and Acido genomes 
LUAcid_AD370_D1_111743716919  hypothetical protein 66RADDED   - thioredoxin domain - best match to Geobacter (28% id) - start changed to 66aa to eliminate overhangPROTEIN  Good but overlappingB82.227.62.18014/09/07 18:43:24- very low match to Acidobacteria in genomes 
LUAcid_AD370_D1_131904117842  hypothetical protein (neuramidase related) 1RADDED   - best match Flavobacterium sp. MED217 (37% id) - length OK - low match in Oryza (Rice) - sialidase/neuramidase domainePROTEIN  GoodB82.227.62.18014/09/07 18:50:55- not in acidobacteria 
LUAcid_AD370_D1_141914719701  translation elongation factor P 17DADDED   - best match Solibacter (63% id) - second Ellin345 - start codon to 17aa adaptation to lenght of othersPROTEINCOG0231 Translation elongation factor P (EF-P)/translation initiation factor 5A (eIF-5A)J Translation, ribosomal structure and biogenesisGood but overlappingB82.227.62.18014/09/07 19:02:29- two matches in each acidobacterial genome - not on clones - but on environmental clone translation elongation factor P [uncultured bacterium 105] locus AAR37444 (Delong) 
LUAcid_AD370_D1_152038819747  hypothetical protein 1RADDED   - low similarity to Tat (twin-arginine translocation) pathway signal sequence domain protein - TAT_signal domain - length OKPROTEIN  GoodB82.227.62.18014/09/07 19:54:05- low similarity to Acid7383 and KM3_B4__029 
LUAcid_AD370_D1_162209320384  glucose-methanol-choline oxidoreductase 1RADDED   - best match Solibacter usitatus Ellin6076 (50% Id) - also COG0765 - NAD and FAD binding domain - Oxidoreductase family - 2 matches also in HalobacteriaceaePROTEINCOG2303 Choline dehydrogenase and related flavoproteinsE Amino acid transport and metabolismGoodB82.227.62.18014/09/07 20:01:02- several matches in geacido genome sequences - match in KM3_25_B4 
LUAcid_AD370_D1_172366422174  glycerol kinase 1RADDED   - best match to Deinococcus geothermalis (55% id) - highly conserved in B - also COG0017 and COG1070 - carbohydrate kinase relatedPROTEINCOG0554 Glycerol kinaseC Energy production and conversionGoodB82.227.62.18014/09/07 20:05:32- two good matches in each genome - not in clones 
LUAcid_AD370_D1_182376024392  conserved hypothetical protein 1DADDED   - best match to Acid3195 (40% id) - predicted hydrolase of alpha/beta superfamilyPROTEINCOG2945 Predicted hydrolase of the alpha/beta superfamilyR General function prediction onlyGoodB82.227.62.18014/09/07 20:11:48- one match in each genome - not on clones - environment not conserved 
LUAcid_AD370_D1_192541624427  acetamidase/formamidase 1RADDED   - best match to Aurantimonas sp. SI85-9A1 (49% id) - conserved proteinPROTEINCOG2421 Predicted acetamidase/formamidaseC Energy production and conversionGoodABE82.227.62.18014/09/07 20:18:26- good matches in Ellin6706 but not in Acid345 
LUAcid_AD370_D1_202597625533  putative ribosomal-protein acetyltransferase 1RADDED   - Burkholderia pseudomallei (32% id) - one match in Methonpyrus (Euryarchaeota) PROTEINCOG0456 AcetyltransferasesR General function prediction onlyGoodB82.227.62.18014/09/07 20:49:40- one match in the two genomes - low similarity to KM3_47_B8_104 
LUAcid_AD370_D1_212619827133  Sulfite oxidase-like enzyme 34DADDED   - best match to uncultured organism (53% id) from ”Construction and analysis of a metagenomic library from a deep-sea sediment of east Pacific nodule Province - next match Methylobacterium - oxidoreductase, molybdopterin binding domain - start codon to 34aa, adaptation to length of others PROTEINCOG2041 Sulfite oxidase and related enzymesR General function prediction onlyGoodB82.227.62.18014/09/07 21:00:08- one good match in the both sequenced genomes and 2 lower matches (45% id) - no match on clones - interesting match from uncultured deep sea 
LUAcid_AD370_D1_222739228273  dTDP-4-dehydrorhamnose reductase 22DADDED 1.1.1.133 - best match Solibacter usitatus (49% id) - start to 22aa - extracellular polysaccharide biosynthetic processPROTEINCOG1091 dTDP-4-dehydrorhamnose reductaseM Cell wall/membrane/envelope biogenesisGoodAB82.227.62.18014/09/07 21:08:50- similar to Acid_6228 - environment not conserved 
LUAcid_AD370_D1_232873728276  single-strand binding protein 13RADDED   - best match to Solibacter usitatus Ellin6076 (51% id)PROTEINCOG0629 Single-stranded DNA-binding proteinL Replication, recombination and repairGoodB82.227.62.18014/09/07 21:54:08- one match in the two genomes - not on clones 
LUAcid_AD370_D1_242964328858  molybdenum ABC transporter molybdenum-binding protein 1RADDED   - best match to Symbiobacterium thermophilum (40% id) - conservedPROTEINCOG0725 ABC-type molybdate transport system, periplasmic componentP Inorganic ion transport and metabolismGoodAB82.227.62.18014/09/07 21:59:06- one match in the two genomes - not in clones 
LUAcid_AD370_D1_252982231030  major facilitator superfamily MFS_1 1DADDED   - best match to Parvibaculum lavamentivorans (23% id)PROTEINCOG0477 Permeases of the major facilitator superfamilyG Carbohydrate transport and metabolismGoodABE82.227.62.18014/09/07 22:05:45- very low match in both genomes 
LUAcid_AD370_D1_263262631142  protein of unknown function DUF1501 1RADDED   - best match to Planctomyces maris DSM 8797 (52% id) - very high number of paralogs in each genome with orthologs (Planctomycetes 64 hits) - only present in a small number of Bacteria...Poribacteria...PROTEIN  GoodB82.227.62.18014/09/07 22:20:17- strong similarities to KM3_18_F8_163 and KM3_18_F8_128 - several paralogs (10+) in Solibacter, not in Elllin345 - often in tandem with DUF1549 protein as on clone KM3_18_F8 ORF128 and 116 - here not in tandem - ortholog on Poribacteria fragment 
LUAcid_AD370_D1_273279634130  glutamate-1-semialdehyde-2,1-aminomutase 1DADDED 5.4.3.8 - best match to Lentisphaera araneosa (47%) - very conserved protein in bacteria and archaea - also COG4992 and COG0160PROTEINCOG0001 Glutamate-1-semialdehyde aminotransferaseH Coenzyme transport and metabolismGoodAB82.227.62.18014/09/07 22:29:17- good match on the two genomes - ortholog to KM3_18_F8_249  
LUAcid_AD370_D1_283422235229  N-acetyl-gamma-glutamyl-phosphate reductase 1DADDED 1.2.1.38 - best match to Solibacter usitatus (62% id) - very conserved in bacteria but also in ArchaeaPROTEINCOG0002 Acetylglutamate semialdehyde dehydrogenaseE Amino acid transport and metabolismGoodAB82.227.62.18014/09/07 22:36:41- one ortholog on the two genomes - not on clones 
LUAcid_AD370_D1_293526336039  acetylglutamate kinase 1DADDED 2.7.2.8 - best match to Solibacter usitatus (51% id) - often in tandem with glutamate-1-semialdehyde-2,1-aminomutase as here with 346PROTEINCOG0548 Acetylglutamate kinaseE Amino acid transport and metabolismGoodAB82.227.62.18014/09/07 22:45:23- one match on the two genomes 
LUAcid_AD370_D1_303629236603  putative regulatory protein, FmdB family 1DADDED   - best match to Solibacter usitatus (55% id)PROTEINCOG2331 Uncharacterized protein conserved in bacteriaS Function unknownGoodB82.227.62.18014/09/07 22:54:56- one good match in the two genomes - lower match in KM3_205_D9_425 
LUAcid_AD370_D1_313757538174  threonyl-tRNA synthetase (truncated c-terminal) 1DADDED   - best match to Acidobacteria bacterium (54% id)PROTEINCOG0441 Threonyl-tRNA synthetaseJ Translation, ribosomal structure and biogenesisGood 82.227.62.18014/09/07 22:58:57- one match in the two genomes 
LUACID_AD370_D1_011595372  ABC efflux pump, inner membrane subunit, DUF214 1RADDED   - best match to Acidobacteria bacterium Ellin345 (35% identity) - matches in Bacteria, not A and not E - also CO0318 IQ lipid transport and metabolism - no matches on other clones - length good start GTGPROTEINCOG0577 ABC-type antimicrobial peptide transport system, permease componentV Defense mechanismsGoodB (only bacteria)82.227.62.18012/10/07 17:19:28  
LUACID_AD370_D1_16SRNA58847399  16S ribosomal RNA 1DADDED16SrRNA   RNA    129.175.105.7529/10/07 17:29:56  
LUACID_AD370_D1_23SRNA805410884  23S ribosomal RNA 1DADDED23SrRNA   RNA    129.175.105.7529/10/07 17:30:20  
LUACID_AD370_D1_5SRNA1114811240  5S ribosomal RNA 1DADDED5SrRNA   RNA    129.175.105.7529/10/07 17:31:01  
LUACID_AD370_D1_TRNA175887660  tRNA-Ala (TGC) 1DADDEDtRNA-Ala   TRNA    129.175.105.7529/10/07 17:32:31  
LUACID_AD370_D1_TRNA276917764  tRNA-Ile (GAT) 1DADDEDtRNA-Ile   TRNA    129.175.105.7529/10/07 17:33:01  
LUACID_AD370_D1_212619827133  oxidoreductase, molybdopterin binding domain 34DADDED   - best match to uncultured organism (53% id) from iConstruction and analysis of a metagenomic library from a deep-sea sediment of east Pacific nodule Province - next match Methylobacterium - oxidoreductase, molybdopterin binding domain - start codon to 34aa, adaptation to length of othersPROTEINCOG2041 Sulfite oxidase and related enzymesR General function prediction onlyGoodB (only bacteria)82.227.62.18029/11/07 09:29:09- one good match in the both sequenced genomes and 2 lower matches (45% id) - no match on clones - interesting match from uncultured deep sea 
LUACID_AD370_D1_141914719701  translation elongation factor P 17DADDEDef-P  - best match Solibacter (63% id) - second Ellin345 - start codon to 17aa adaptation to lenght of othersPROTEINCOG0231 Translation elongation factor P (EF-P)/translation initiation factor 5A (eIF-5A)J Translation, ribosomal structure and biogenesisGood but overlappingB (only bacteria)129.175.105.7530/01/08 17:48:19- two matches in each acidobacterial genome - not on clones - but on environmental clone translation elongation factor P [uncultured bacterium 105] locus AAR37444 (Delong)