Current Annotation for L35504_KM3_187_G1 20/11/17

namestartcoordendcoord**deintronstart1directionstatusgnecgpcctype1kognumkogglimmerdompatauth10/10/03 00:00:00More Comments 
                    10/10/03 00:00:00  
LUAcid_KM3_G1_013581194  beta-lactamase-like protein 19DADDED   - best match euryarchaeote: eta-lactamase domain protein, Candidatus Methanoregula boonei 6A8 (39% id), then bacteria - also COG0324 - seems to be part of purin operon - start codon changed to 19aaPROTEINCOG1234 Metal-dependent hydrolases of the beta-lactamase superfamily IIIR General function prediction onlyGoodBE82.227.62.18018/09/07 11:01:17- paralogs on different clones LUAcid_KM3_G1_01 LUAcid_KM3_D9_13 LUAcid_AD106_G4_08 L35498_AD55_D2_360 LUAcid_KM4_H7_24 
LUAcid_KM3_G1_0212062516  adenylosuccinate lyase (PurB) 1DADDED   - best match to KM3_205_D9_282 (80% id) - next match 41b15 (69% id) - purine ribonucleotide biosynthetic process PROTEINCOG0015 Adenylosuccinate lyaseF Nucleotide transport and metabolismGoodABE82.227.62.18018/09/07 11:34:00- orthologs found in other clones: LUAcid_KM3_G1_02 LUAcid_KM3_D9_14 L35498_AD55_D2_353 LUAcid_AD106_G4_09 LUAcid_KM4_H7_25 23k22_016 PurB (AY281356 soil clone) 41b15_012 PurB (AY281352 soil clone)  
LUAcid_KM3_G1_0325162752  phosphoribosylformylglycinamidine synthase I (PurS) 1DADDED   - best match to AD55_D2_340 (69% id) - purin biosynthesis pathwayPROTEINCOG1828 Phosphoribosylformylglycinamidine (FGAM) synthase, PurS componentF Nucleotide transport and metabolism-AB82.227.62.18018/09/07 11:49:06- orthologs on other acidobacterial clones: LUAcid_KM3_G1_03 L35498_AD55_D2_340 LUAcid_KM3_D9_15 LUAcid_AD106_G4_10 LUAcid_KM4_H7_26 23k22_011 PurS AY281352 41b15_015 PurS AY281356 
LUAcid_KM3_G1_0427523468  phosphoribosylformylglycinamidine synthase I (PurQ) 1DADDED   best match to KM3_205_D9_262 (84% id) - Purin biosynthesis operonPROTEINCOG0047 Phosphoribosylformylglycinamidine (FGAM) synthase, glutamine amidotransferase domainF Nucleotide transport and metabolismGoodAB82.227.62.18018/09/07 13:10:46- several orthologs in Acidobacteria LUAcid_KM3_G1_04 LUAcid_KM3_D9_16 L35498_AD55_D2_336 LUAcid_AD106_G4_11 LUAcid_KM4_H7_27 41b15_014 PurQ AY281356 23k22_010 PurQ AY281352 Acid345_2235 Acid_5943 
LUAcid_KM3_G1_0534883694  hypothetical protein 19DADDED   no hitsPROTEIN  Good 82.227.62.18018/09/07 13:42:47  
LUAcid_KM3_G1_0636945910  phosphoribosylformylglycinamidine synthase II (PurL) 1DADDED 6.3.5.3 best match to KM3_205_D9_254 (77% id) - purin biosynthesis operon - AIR synthase related protein - ’de novo’ IMP biosynthetic process - hosphoribosylformylglycinamidine synthase is a single, long polypeptide in most bacteria. Three proteins are required in Bacillus subtilis and many other species. This is the longest of the three and is designated PurL, phosphoribosylformylglycinamidine synthase II, or FGAM synthase II.PROTEINCOG0046 Phosphoribosylformylglycinamidine (FGAM) synthase, synthetase domainF Nucleotide transport and metabolismGoodAB82.227.62.18018/09/07 14:08:01several orthologs in Acidobacteria: LUAcid_KM3_G1_06 LUAcid_KM3_D9_17 L35498_AD55_D2_328 LUAcid_AD106_G4_12 LUAcid_KM4_H7_28 L35511_KM3_47_B8_428 23k22_009 PurL AY281352 41b15_013 PurL AY281356 Acid_5944 Acid345_4622 
LUAcid_KM3_G1_212922928543  lipolytic secreted GDSL family protein 1RADDED 3.1 - best match to Planctomyces maris DSM 8797 (44% id) - A variety of lipolytic enzymes with serine as part of the active site have been identified [1]. Members of this family include; Aeromonas hydrophila lipase, Vibrio mimicus arylesterase, Vibrio parahaemolyticus thermolabile hemolysin, rabbit phospholipase (AdRab-B), and Brassica napus anter-specific proline-rich protein. - also in some ArchaeaPROTEINCOG2755 Lysophospholipase L1 and related esterasesE Amino acid transport and metabolismGoodBE82.227.62.18018/09/07 15:08:33- 3 matches in Solibacter (Acid_7819, Acid_2072 - no matches in other clones 
LUAcid_KM3_G1_0759107337  amidophosphoribosyl transferase (PurF) 1DADDED 2.4.2.14 - best match to KM3_205_D9_226 (88% id) - purin biosynthesis operon - also COG0449 - Purine nucleotides are synthesised both via the de novo pathway and via the salvage pathway and are vital for cell functions and cell proliferation through DNA and RNA syntheses and ATP energy supply. Amidophosphoribosyltransferase (EC:2.4.2.14) is the rate-limiting enzyme in the de novo pathway of purine ribonucleotide synthesis and is regulated by feedback inhibition by AMP and GMP [1].PROTEINCOG0034 Glutamine phosphoribosylpyrophosphate amidotransferaseF Nucleotide transport and metabolismGoodABE82.227.62.18018/09/07 15:32:39several othologs in Acidobacteria LUAcid_KM3_G1_07 LUAcid_KM3_D9_18 L35511_KM3_47_B8_405 L35498_AD55_D2_302 LUAcid_AD106_G4_13 LUAcid_KM4_H7_29 41b15_012 PurF AY281356 23k22_008 PurF AY281352 Acid345_4621 Acid_5945 
LUAcid_KM3_G1_243234033149  oxidoreductases of the aldo/keto reductase family-like 1DADDED   best match to Candidatus Desulfococcus oleovorans Hxd3 (30% id) - no COG - InterPro001395 Aldo/keto reductase family - predicted oxidoreductase activityPROTEIN  GoodAB82.227.62.18018/09/07 16:39:21low matches in both acidobacterial genomes - not in clones 
LUAcid_KM3_G1_0873378536  hypothetical protein 1DADDED   - no COGPROTEIN  Good 82.227.62.18021/09/07 09:22:57- best matches to Acidobacteria, also soil clone - environment conserved - to be verified 
LUAcid_KM3_G1_0985459594  phosphoribosylformylglycinamidine cyclo ligase (PurM) 1DADDED   - purin biosynthesis operonPROTEINCOG0150 Phosphoribosylaminoimidazole (AIR) synthetaseF Nucleotide transport and metabolismGood 82.227.62.18021/09/07 09:27:38- best matches to Acidobacteria, also soil clone - environment conserved - to be verified 
LUAcid_KM3_G1_10961510220  phosphoribosylglycinamide formyltransferase (PurN) 1DADDED   - purin biosynthesis operonPROTEINCOG0299 Folate-dependent phosphoribosylglycinamide formyltransferase PurNF Nucleotide transport and metabolismGood 82.227.62.18021/09/07 09:35:28- environment conserved in Acidobacteria - also in soil clones - to be verified 
LUAcid_KM3_G1_111040011614  tyrosyl-tRNA synthetase (TyrS) 1DADDED 6.1.1.1  PROTEINCOG0162 Tyrosyl-tRNA synthetaseJ Translation, ribosomal structure and biogenesisGood 82.227.62.18021/09/07 09:39:11- environment conserved in Acidobacteria - also in soil clones - to be verified 
LUAcid_KM3_G1_121767818412  hypothetical protein 1DADDED   - no COG PROTEIN  Good 82.227.62.18021/09/07 09:45:19- one match to Acid345_1727 - environment conserved in acidobacterial clones - to be verified 
LUAcid_KM3_G1_131844020029  hypothetical protein 1DADDED   - no COG PROTEIN  Good 82.227.62.18021/09/07 09:46:18- one match to acidobacterial clones - environment conserved in acidobacterial clones - to be verified 
LUAcid_KM3_G1_131844020029  hypothetical protein 1DADDED   - no COG PROTEIN  Good 82.227.62.18021/09/07 09:48:10- one match to acidobacterial clones - environment conserved in acidobacterial clones - to be verified 
LUAcid_KM3_G1_142003221477  FAD linked oxidase 1DADDED   - best match Rubrobacter xylanophilus - very conserved 56% identity - D-lactat dehydrogenase (cytochrome) - FAD/FMN- containing dehydrogenase (GlcD)PROTEIN  Good 82.227.62.18021/09/07 09:50:09- environment conserved in Acidobacteria - to be verified 
LUAcid_KM3_G1_142003221477  FAD linked oxidase 1DADDED   - best match Rubrobacter xylanophilus - very conserved 56% identity - D-lactat dehydrogenase (cytochrome) - FAD/FMN- containing dehydrogenase (GlcD)PROTEINCOG0277 FAD/FMN-containing dehydrogenasesC Energy production and conversionGood 82.227.62.18021/09/07 09:53:12- environment conserved in Acidobacteria - to be verified 
LUAcid_KM3_G1_152147722760  FAD linked oxidase-like protein 1DADDED    PROTEINCOG0277 FAD/FMN-containing dehydrogenasesC Energy production and conversionGood 82.227.62.18021/09/07 09:59:25- environment conserved in Acidobacteria - to be verified 
LUAcid_KM3_G1_162416422767  protein of unknown function DUF224, cysteine-rich region 1RADDED    PROTEIN C Energy production and conversionGood 82.227.62.18021/09/07 10:02:00- environment conserved in Acidobacteria - to be verified 
LUAcid_KM3_G1_172405125220  NADPH:quinone reductase 1DADDED   - electron transport operon like structurePROTEINCOG0604 NADPH:quinone reductase and related Zn-dependent oxidoreductasesC Energy production and conversionGood 82.227.62.18021/09/07 10:04:46- environment conserved in Acidobacteria - to be verified 
LUAcid_KM3_G1_192572627600  gamma-glutamyltranspeptidase 1DADDED    PROTEINCOG0405 Gamma-glutamyltransferaseE Amino acid transport and metabolismGood 82.227.62.18021/09/07 10:06:38- environment conserved in Acidobacteria - to be verified 
LUAcid_KM3_G1_182524325719  Endoribonuclease L-PSP 1DADDED   - also COG0024 - length in other variable, but OkPROTEINCOG0251 Putative translation initiation inhibitor, yjgF familyJ Translation, ribosomal structure and biogenesisGood 82.227.62.18021/09/07 10:10:45- environment conserved in Acidobacteria - to be verified 
LUAcid_KM3_G1_202763728434  Sulphate-modifying factor-like protein 1DADDED   protein of unknown function DUF323 - best matches to EPROTEINCOG1262 Uncharacterized conserved proteinS Function unknownGood 82.227.62.18021/09/07 10:15:12- environment conserved in other Acidobacteria - to be verified 
LUAcid_KM3_G1_222942031372  TPP-binding enzymes domain protein 1DADDED   - no COG - IPR000399 TPP-binding enzymes; big group of proteins: A number of enzymes require thiamine pyrophosphate (TPP) (vitamin B1) as a cofactor. It has been shown [1] that some of these enzymes are structurally relatedPROTEIN  Good 82.227.62.18021/09/07 10:18:24- environment conserved in Acidobacteria - to be verified 
LUAcid_KM3_G1_233184532327  - hypothetical protein 1DADDED   - best match to uncultured bacterium BAC10-10 40% id) - no COG - length Ok, - not very conserved - IPR003219 Cytochrome c, alcohol dehydrogenase-like subunit: Cytochromes c (cytC) can be defined as electron-transfer proteins having one or several haem c groups, bound to the protein by one or, more generally, two thioether bonds involving sulphydryl groups of cysteine residues. The fifth haem iron ligand is always provided by a histidine residue. CytC possess a wide range of properties and function in a large number of different redox processes [1]. - electron tranfer related?PROTEIN  Good 82.227.62.18021/09/07 10:22:22- environment conserved in Acidobacteria - to be verified 
LUACID_KM3_G1_233184532327  - hypothetical protein 20DADDED   - best match to uncultured bacterium BAC10-10 40% id) - no COG - length Ok, - not very conserved - IPR003219 Cytochrome c, alcohol dehydrogenase-like subunit: Cytochromes c (cytC) can be defined as electron-transfer proteins having one or several haem c groups, bound to the protein by one or, more generally, two thioether bonds involving sulphydryl groups of cysteine residues. The fifth haem iron ligand is always provided by a histidine residue. CytC possess a wide range of properties and function in a large number of different redox processes [1]. - electron tranfer related?PROTEIN  Good 129.175.107.20410/10/07 17:14:33- environment conserved in Acidobacteria - to be verified 
LUACID_KM3_G1_16SRNA1191913482  16S ribosomal RNA 1DADDED16SrRNA   RNA    129.175.105.7529/10/07 17:39:52  
LUACID_KM3_G1_23SRNA1417517054  23S ribosomal RNA 1DADDED23SrRNA   RNA    129.175.105.7529/10/07 17:40:08  
LUACID_KM3_G1_5SRNA1732317429  5S ribosomal RNA 1DADDED5SrRNA   RNA    129.175.105.7529/10/07 17:40:40  
LUACID_KM3_G1_TRNA11359513668  tRNA-Ile (GAT) 1DADDEDtRNA-Ile   TRNA    129.175.105.7529/10/07 17:41:26  
LUACID_KM3_G1_TRNA21385313925  tRNA-Ala (TGC) 1DADDEDtRNA-Ala   TRNA    129.175.105.7529/10/07 17:42:19  
LUACID_KM3_G1_162416422767  protein of unknown function DUF224, cysteine-rich region 1RADDED    PROTEINCOG0247 Fe-S oxidoreductaseC Energy production and conversionGood 129.175.105.7517/12/07 16:37:59- environment conserved in Acidobacteria - to be verified fixed COG -yz