Current Annotation for L35507_KM3_205_D9 19/04/24

namestartcoordendcoord**deintronstart1directionstatusgnecgpcctype1kognumkogglimmerdompatauth10/10/03 00:00:00More Comments 
                    10/10/03 00:00:00  
LUAcid_KM3_D9_131160712443  beta-lactamase-like protein 1DADDED   - best match euryarchaeote: eta-lactamase domain protein, Candidatus Methanoregula boonei 6A8 (40% id), then bacteria - also COG0324 - seems to be part of purin operonPROTEINCOG1235 Metal-dependent hydrolases of the beta-lactamase superfamily IR General function prediction onlyGoodBE82.227.62.18018/09/07 09:15:26- paralogs on different clones LUAcid_KM3_G1_01 LUAcid_KM3_D9_13 LUAcid_AD106_G4_08 L35498_AD55_D2_360 LUAcid_KM4_H7_24 
LUAcid_KM3_D9_141245513765  adenylosuccinate lyase (PurB) 1DADDED   - best match to KM3_187_G1_032 (81% id) - next match 23k22_016 (71% id) - purine ribonucleotide biosynthetic processPROTEINCOG0015 Adenylosuccinate lyaseF Nucleotide transport and metabolismGoodABE82.227.62.18018/09/07 11:24:15- orthologs found in other clones: LUAcid_KM3_G1_02 LUAcid_KM3_D9_14 L35498_AD55_D2_353 LUAcid_AD106_G4_09 LUAcid_KM4_H7_25 23k22_016 PurB (AY281356 soil clone) 41b15_012 PurB (AY281352 soil clone)  
LUAcid_KM3_D9_151376514001  phosphoribosylformylglycinamidine synthase I (PurS) 1DADDED   - best match to AD55_D2_340 (81% id) - purin biosynthesis pathwayPROTEINCOG1828 Phosphoribosylformylglycinamidine (FGAM) synthase, PurS componentF Nucleotide transport and metabolism-AB82.227.62.18018/09/07 11:44:17- orthologs on other acidobacterial clones: LUAcid_KM3_G1_03 L35498_AD55_D2_340 LUAcid_KM3_D9_15 LUAcid_AD106_G4_10 LUAcid_KM4_H7_26 23k22_011 PurS AY281352 41b15_015 PurS AY281356 
LUAcid_KM3_D9_171481017026  phosphoribosylformylglycinamidine synthase II (PurL) 1DADDED 6.3.5.3 best match to KM3_187_G1_074 (76% id) - purin biosynthesis operon - AIR synthase related protein - ’de novo’ IMP biosynthetic process - hosphoribosylformylglycinamidine synthase is a single, long polypeptide in most bacteria. Three proteins are required in Bacillus subtilis and many other species. This is the longest of the three and is designated PurL, phosphoribosylformylglycinamidine synthase II, or FGAM synthase II.PROTEINCOG0046 Phosphoribosylformylglycinamidine (FGAM) synthase, synthetase domainF Nucleotide transport and metabolismGoodAB82.227.62.18018/09/07 13:54:06several orthologs in Acidobacteria: LUAcid_KM3_G1_06 LUAcid_KM3_D9_17 L35498_AD55_D2_328 LUAcid_AD106_G4_12 LUAcid_KM4_H7_28 L35511_KM3_47_B8_428 23k22_009 PurL AY281352 41b15_013 PurL AY281356 Acid_5944 Acid345_4622 
LUAcid_KM3_D9_181704018503  amidophosphoribosyl transferase (PurF) 1DADDED 2.4.2.14 - best match to KM3_187_G1_086 (88% id) - purin biosynthesis operon - also COG0449PROTEINCOG0034 Glutamine phosphoribosylpyrophosphate amidotransferaseF Nucleotide transport and metabolismGoodABE82.227.62.18018/09/07 15:34:08several othologs in Acidobacteria LUAcid_KM3_G1_07 LUAcid_KM3_D9_18 L35511_KM3_47_B8_405 L35498_AD55_D2_302 LUAcid_AD106_G4_13 LUAcid_KM4_H7_29 41b15_012 PurF AY281356 23k22_008 PurF AY281352 Acid345_4621 Acid_5945 
LUAcid_KM3_D9_0965427591  YVTN repeat-like/Quinoprotein amine dehydrogenase 1DADDED   - best match to Acid_2447 (46% id) - IPR001258 NHL repeat subgroup - motive in Proteases and monooxygenasesPROTEINCOG3391 Uncharacterized conserved proteinS Function unknownGoodABE82.227.62.18018/09/07 16:49:30- one match to Solibacter, not Ellin6706 
LUAcid_KM3_D9_191850319705  hypothetical protein 1DADDED   - no COGPROTEIN  Good 82.227.62.18021/09/07 09:23:38- best matches to Acidobacteria, also soil clone - environment conserved - to be verified 
LUAcid_KM3_D9_201972220756  phosphoribosylformylglycinamidine cyclo ligase (PurM) 1DADDED   - purin biosynthesis operonPROTEINCOG0150 Phosphoribosylaminoimidazole (AIR) synthetaseF Nucleotide transport and metabolismGood 82.227.62.18021/09/07 09:28:42- best matches to Acidobacteria, also soil clone - environment conserved - to be verified 
LUAcid_KM3_D9_212078321382  phosphoribosylglycinamide formyltransferase (PurN) 1DADDED   purin biosynthesis operonPROTEINCOG0299 Folate-dependent phosphoribosylglycinamide formyltransferase PurNF Nucleotide transport and metabolismGood 82.227.62.18021/09/07 09:34:29- environment conserved in Acidobacteria - also in soil clones - to be verified 
LUAcid_KM3_D9_222147422715  tyrosyl-tRNA synthetase (TyrS) 1DADDED 6.1.1.1  PROTEINCOG0162 Tyrosyl-tRNA synthetaseJ Translation, ribosomal structure and biogenesisGoodABE82.227.62.18021/09/07 09:38:05- environment conserved in acidobacteria 
LUAcid_KM3_D9_232851029244  hypothetical protein 1DADDED   - no COGPROTEIN  Good 82.227.62.18021/09/07 09:45:05- one match to Acid345_1727 - environment conserved in acidobacterial clones - to be verified 
LUAcid_KM3_D9_253028431792  hypothetical protein 1DADDED   - no COGPROTEIN  Good 82.227.62.18021/09/07 09:47:59- one match to acidobacterial clones - environment conserved in acidobacterial clones - to be verified 
LUAcid_KM3_D9_263183233277  FAD linked oxidase 1DADDED   identity - D-lactat dehydrogenase (cytochrome) - FAD/FMN- containing dehydrogenase (GlcD)PROTEINCOG0277 FAD/FMN-containing dehydrogenasesC Energy production and conversionGood 82.227.62.18021/09/07 09:51:41- environment conserved in Acidobacteria - to be verified 
LUAcid_KM3_D9_273328034560  FAD linked oxidase-like protein 1DADDED    PROTEINCOG0277 FAD/FMN-containing dehydrogenasesC Energy production and conversionGood 82.227.62.18021/09/07 09:59:40- environment conserved in Acidobacteria - to be verified 
LUAcid_KM3_D9_283590934584  protein of unknown function DUF224, cysteine-rich region 1RADDED    PROTEIN C Energy production and conversionGood 82.227.62.18021/09/07 10:02:25- environment conserved in Acidobacteria - to be verified 
LUAcid_KM3_D9_293602637003  NADPH:quinone reductase 1DADDED   - electron transport operon like structurePROTEINCOG0604 NADPH:quinone reductase and related Zn-dependent oxidoreductasesC Energy production and conversionGood 82.227.62.18021/09/07 10:04:58- environment conserved in Acidobacteria - to be verified 
LUAcid_KM3_D9_303702437491  Endoribonuclease L-PSP 1DADDED   - also COG0024PROTEINCOG0251 Putative translation initiation inhibitor, yjgF familyJ Translation, ribosomal structure and biogenesisGood 82.227.62.18021/09/07 10:08:37- environment conserved in Acidobacteria - to be verified 
LUAcid_KM3_D9_161400114708  phosphoribosylformylglycinamidine synthase I (PurQ) 1DADDED   - purin biosynthesis operonPROTEINCOG0047 Phosphoribosylformylglycinamidine (FGAM) synthase, glutamine amidotransferase domainF Nucleotide transport and metabolismGood 82.227.62.18021/09/07 10:36:51- environment conserved in Acidobacteria - to be verified 
LUAcid_KM3_D9_243022229254  Alcohol dehydrogenase GroES domain protein 1RADDED   - best match to Crenarchaeota: Candidatus Nitrosopumilus maritimus SCM1 (49% id) - IPR002085 Alcohol dehydrogenase superfamily, zinc-containingPROTEINCOG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenasesE Amino acid transport and metabolismGoodA82.227.62.18023/09/07 17:10:22- several matches in both acidobacterial genomes - orthologs in acidobacterial clones - to be verified 
LUAcid_KM3_D9_121031111567  peptidase M16 domain protein 1DADDED    PROTEINCOG0612 Predicted Zn-dependent peptidasesR General function prediction onlyGood 82.227.62.18024/09/07 08:01:29- matches to acidobacterial clones - to be verified - near to purin biosynthesis operon? 
LUAcid_KM3_D9_11101629038  radical SAM enzyme, Cfr family 1RADDED   - also IPR004383 Conserved hypothetical protein 48 - matches in Bacteria and a few in EPROTEINCOG0820 Predicted Fe-S-cluster redox enzymeR General function prediction onlyGood but overlappingB82.227.62.18024/09/07 08:05:50- matches in acidobacterial clones - environment conserved in acidobacteria - to be verified 
LUAcid_KM3_D9_1077199011  MiaB-like tRNA modifying enzyme 1DADDED   - also IPR005839 Protein of unknown function UPF0004PROTEINCOG0621 2-methylthioadenine synthetaseJ Translation, ribosomal structure and biogenesisGood 82.227.62.18024/09/07 08:08:04- matches in acidobacterial clones - environment conserved in acidobacteria - to be verified 
LUAcid_KM3_D9_0864234915  Na+/H+ antiporter NhaC 1RADDED   - IPR004770 Na+/H+ antiporter NhaCPROTEINCOG1757 Na+/H+ antiporterC Energy production and conversionGoodB82.227.62.18024/09/07 08:11:43- matches in acidobacterial clones G4 and D2; match in Solibacter, not in Acid Ellin6076 - environment conserved in acidobacteria - to be verified 
LUAcid_KM3_D9_0742364874  hypothetical protein 1DADDED   - no COGPROTEIN  Good 82.227.62.18024/09/07 08:16:54- matches to KM3_205_D9_412 and 373 on the same clone paralog? - also similarity to AD106_G4_503 - environment conserved in acidobacteria? - to be verified 
LUAcid_KM3_D9_0311351707  hypothetical protein 1DADDED    PROTEIN  Good 82.227.62.18024/09/07 08:19:17- matches to KM3_205_D9_412 and 373 on the same clone paralog? - also similarity to AD106_G4_503 - environment conserved in acidobacteria? - to be verified 
LUAcid_KM3_D9_0641723198  hypothetical protein 1RADDED   - no COG - very few matchesPROTEIN  Good 82.227.62.18024/09/07 08:22:18- low match to 41b15_006 
LUAcid_KM3_D9_0521833181  ApbE family lipoprotein 1DADDED   - Thiamin biosynthesis - This prokaryotic family of lipoproteins are related to ApbE, from Salmonella typhimurium. ApbE is involved in thiamine synthesis [1]. More specifically is may be involved in the conversion of aminoimidazole ribotide (AIR) to 4-amino-5-hydroxymethyl-2-methyl pyrimidine (HMP) during the biosynthesis of the pyrimidine moiety of thiamine.PROTEINCOG1477 Membrane-associated lipoprotein involved in thiamine biosynthesisH Coenzyme transport and metabolismGoodB82.227.62.18024/09/07 08:30:17- ortholog to AD106_G4_021 (57% id) - low matches in Solibacter, not in Acidobacterium 
LUAcid_KM3_D9_0417072183  hypothetical protein 1DADDED   - no matches - no COG - no InterProPROTEIN  Good 82.227.62.18024/09/07 08:33:47- ortholog to AD106_G4_007 (77% id) - environment conserved on the two clones? - to be verified 
LUAcid_KM3_D9_022011121  hypothetical protein (truncated) 1DADDED   - N-terminal truncated of ca. 120aa - best match to hypothetical protein Anae109_2247 Anaeromyxobacter sp. Fw109-5 (28% id)PROTEIN  Good 82.227.62.18024/09/07 08:38:51- no matches to Acidobacteria 
LUAcid_KM3_D9_0114343  hypothetical protein 1DADDED   - no matches with similar lengthPROTEIN  - 82.227.62.18024/09/07 08:44:58- only one match in Acidobacteria: Acid345_0034 type I antifreeze protein but only on a short region 
LUACID_KM3_D9_16SRNA2301724585  16S ribosomal RNA 1DADDED16SrRNA   RNA    129.175.105.7529/10/07 17:48:55  
LUACID_KM3_D9_23SRNA2508527962  23S ribosomal RNA 1DADDED23SrRNA   RNA    129.175.105.7529/10/07 17:49:36  
LUACID_KM3_D9_5SRNA2821928341  5S ribosomal RNA 1DADDED5SrRNA   RNA    129.175.105.7529/10/07 17:49:53  
LUACID_KM3_D9_TRNA12469724770  tRNA-Ile (GAT) 1DADDEDtRNA-Ile   TRNA    129.175.105.7529/10/07 17:50:51  
LUACID_KM3_D9_TRNA22478524857  tRNA-Ala (TGC) 1DADDEDtRNA-Ala   TRNA    129.175.105.7529/10/07 17:51:57  
LUACID_KM3_D9_293602637003  alcohol dehydrogenase, GroES and Zn-binding domain 1DADDED   - electron transport operon like structurePROTEINCOG0604 NADPH:quinone reductase and related Zn-dependent oxidoreductasesC Energy production and conversionGood 82.227.62.18013/12/07 17:33:22- environment conserved in Acidobacteria - to be verified 
LUACID_KM3_D9_263183233277  Glycolate oxidase subunit (GlcD) 1DADDED   identity - D-lactat dehydrogenase (cytochrome) - FAD/FMN- containing dehydrogenase (GlcD)PROTEINCOG0277 FAD/FMN-containing dehydrogenasesC Energy production and conversionGood 82.227.62.18013/12/07 17:34:41- environment conserved in Acidobacteria - to be verified 
LUACID_KM3_D9_273328034560  Glycolate oxidase subunit (GlcE) 1DADDED    PROTEINCOG0277 FAD/FMN-containing dehydrogenasesC Energy production and conversionGood 82.227.62.18013/12/07 17:35:24- environment conserved in Acidobacteria - to be verified 
LUACID_KM3_D9_283590934584  Glycolate oxidase subunit (GlcF) 1RADDED    PROTEIN C Energy production and conversionGood 82.227.62.18013/12/07 17:35:58- environment conserved in Acidobacteria - to be verified 
LUACID_KM3_D9_283590934584  Glycolate oxidase subunit (GlcF) 1RADDEDGlcF   PROTEINCOG0247 Fe-S oxidoreductaseC Energy production and conversionGood 129.175.105.7517/12/07 16:35:47- environment conserved in Acidobacteria - to be verified fixed COG/ gN -yz