Current Annotation for L35511_KM3_47_B8 20/09/17

LUAcid_KM3_B8_364535443354  hypothetical protein 1RADDED   - outer membran autotransporter barrel domain - Autotransporter-associated beta strand repeat - lenth to matches very differentPROTEINCOG3468 Type V secretory pathway, adhesin AidAM Cell wall/membrane/envelope biogenesisGood 82.227.62.18026/09/07 07:27:50- low matches in both acidobacterial genomes with different length 
LUAcid_KM3_B8_344274443337  Holliday junction DNA helicase RuvA 1DADDED   - IPR000085 Bacterial DNA recombination protein RuvA - also COG0022PROTEINCOG0632 Holliday junction resolvasome, DNA-binding subunitL Replication, recombination and repairGoodB82.227.62.18026/09/07 07:32:19- match to AD106_G4_452 (66% id) and one in both genomes - environment conserved in Acidobacteria? - to be verified 
LUAcid_KM3_B8_334217742728  crossover junction endodeoxyribonuclease RuvC 1DADDED   - IPR002176 Crossover junction endodeoxyribonuclease RuvC PROTEINCOG0817 Holliday junction resolvasome, endonuclease subunitL Replication, recombination and repairGood but overlappingB82.227.62.18026/09/07 07:34:59- match to AD106_G4_447 (58% id) and one in both genomes - environment conserved in Acidobacteria? - to be verified 
LUAcid_KM3_B8_324141642177  protein of unknown function DUF28 1DADDED   DUF28 domain in B and E, rare in APROTEINCOG0217 Uncharacterized conserved proteinS Function unknownGood 82.227.62.18026/09/07 07:38:44- match to AD106_G4__439 (77% id) and one in both genomes - environment conserved in Acidobacteria? - to be verified 
LUAcid_KM3_B8_314051041265  Methyltransferase type 11 1DADDED   - no COG - best match Cloroflexus only 27% id - low match to COG2226, UbiE, Methylase involved in ubiquinone/menaquinone biosynthesis - IPR013216 Methyltransferase type 11 - matches to CrenarcheaePROTEIN  Good 82.227.62.18026/09/07 07:48:31- match to AD106_G4_433 (N-terminal) and 430 (C-terminal); probable frameshift between 433 and 430 - no matches in both genomes - environment conserved in AD106_G4 
LUAcid_KM3_B8_304039139888  hypothetical membrane proteine 1RADDED   - best match to hypothetical protein SRU_0577 Salinibacter ruber DSM 13855 (36% id) - no COG - low match to COG4244, COG4244, Predicted membrane protein [Function unknown]. - no interPro familyPROTEIN  Good 82.227.62.18026/09/07 07:58:10- match to AD106_G4_424 (42% id) - no match in both acidobacterial genomes - environment conserved in clones? - to be verified 
LUAcid_KM3_B8_293988837786  quinoprotein glucose dehydrogenase 1RADDED   - best match to quinate/shikimate dehydrogenase, putative marine gamma proteobacterium HTCC2143 (41% id) - orthologs affiliated to proteobacteria and few other bacteria - IPR011047 Quinonprotein alcohol dehydrogenase-like: Quinoprotein alcohol dehydrogenases are a family of proteins found in methylotrophic or autotrophic bacteria - large family in B, A and EPROTEINCOG4993 Glucose dehydrogenaseG Carbohydrate transport and metabolismGoodB82.227.62.18026/09/07 08:08:48- several matches to Solibacter (best 42% id), not to Ellin345 strain - high number of matches in Solibacter, but only some in other bacterial genomes - match to KM3_25_B4_257 and KM3_25_B4_153 - environment conserved? - to be verified 
LUAcid_KM3_B8_283723537783  acetyltransferase (GNAT) family protein 26DADDED   - best match to acetyltransferase (GNAT) family protein Desulfovibrio desulfuricans G20 (41% id)PROTEINCOG0454 Histone acetyltransferase HPA2 and related acetyltransferasesK TranscriptionGoodAB82.227.62.18026/09/07 08:16:46- no matches to acidobacterial genomes - match to KM3_25_B4_414 - environment conserved? - to be verified 
LUAcid_KM3_B8_273678637271  hypothetical protein 20DADDED   - no COG - IPR003219 Cytochrome c, alcohol dehydrogenase-like subunit - best match to uncultured bacterium BAC10-10 (44% id) PROTEIN  Good but overlappingB82.227.62.18026/09/07 08:26:14- low matches to acidobacterial genomes - match to KM3_187_G1_367 (64% id - environment conserved? - to be verified 
LUAcid_KM3_B8_263635336856  hypothetical protein 1DADDED   - no matches - specific to Acidobacteria - no COG - no familyPROTEIN  Goodunique82.227.62.18026/09/07 08:50:05- best match to KM3_187_G1_360 (57% id) - environment conserved? - to be verified 
LUAcid_KM3_B8_253435636341  thiamine pyrophosphate-requiring enzyme 1DADDED   - IPR000399 TPP-binding enzymes - Thiamine diphosphate-dependent enzyme, acetolactate synthase type domain: A number of enzymes require thiamine pyrophosphate (TPP) (vitamin B1) as a cofactor - no COG - best matches with different length - start codon? - large familyPROTEIN  GoodABE82.227.62.18026/09/07 08:58:42- best match to KM3_187_G1_355 (82% id) - environment conserved? - to be verified 
LUAcid_KM3_B8_243334734231  protein of unknown function DUF323 11DADDED   - conserved hypothetical proteinPROTEINCOG1262 Uncharacterized conserved proteinS Function unknownGood 82.227.62.18026/09/07 09:07:41- best match to KM3_187_G1_318 (72% id) - environment conserved? - to be verified 
LUAcid_KM3_B8_233146233384  gamma-glutamyltransferase 1DADDED   - also COG0075PROTEINCOG0405 Gamma-glutamyltransferaseE Amino acid transport and metabolismGood 82.227.62.18026/09/07 09:10:58- matches on different acidobacterial clones - environment conserved? - to be verified 
LUAcid_KM3_B8_223095531446  Endoribonuclease L-PSP 9DADDED   - also COG0251 - best match to Ellin345PROTEINCOG0024 Methionine aminopeptidaseJ Translation, ribosomal structure and biogenesisGood 82.227.62.18026/09/07 09:32:46- matches on different acidobacterial clones - environment conserved? - to be verified 
LUAcid_KM3_B8_212997330950  Alcohol dehydrogenase superfamily, zinc-containing 1DADDED   - best match to Alcohol dehydrogenase, zinc-binding domain protein Alkalilimnicola ehrlichei MLHE-1 (60% id) - large and conserved familyPROTEINCOG0604 NADPH:quinone reductase and related Zn-dependent oxidoreductasesC Energy production and conversionGoodB82.227.62.18026/09/07 10:04:25- several matches on acidobacterial clones and genomes - environment conserved? - to be verified 
LUAcid_KM3_B8_202995828591  glycolate oxidase, iron-sulphur subunit family protein 1RADDED   - IPR012257 Glycolate oxidase, iron-sulphur subunit - best match 57% idPROTEINCOG0247 Fe-S oxidoreductaseC Energy production and conversionGoodB82.227.62.18027/09/07 08:09:13- several matches to acidobacteria clones - one match to Solibacter - environment conserved? - to be verified 
LUAcid_KM3_B8_192730128566  FAD linked oxidase-like protein 1DADDED   - IPR004113 FAD linked oxidase, C-terminal and IPR006094 FAD linked oxidase, N-terminal - best match 37% id - one match to APROTEINCOG0277 FAD/FMN-containing dehydrogenasesC Energy production and conversionGoodB82.227.62.18027/09/07 08:13:38- several matches to acidobacteria clones - two matches on the same clone, paralog? - two matches to Solibacter - environment conserved? - to be verified 
LUAcid_KM3_B8_182584327291  FAD linked oxidase-like protein 1DADDED   - IPR004113 FAD linked oxidase, C-terminal and IPR006094 FAD linked oxidase, N-terminal - several domain matches - IPR004490 Glycolate oxidase subunit GlcD - best match 55% id - interestin study: Succession and diel transcriptional response of the glycolate-utilizing component of the bacterial community during a spring phytoplankton bloomPROTEINCOG0277 FAD/FMN-containing dehydrogenasesC Energy production and conversionGoodB82.227.62.18027/09/07 08:21:04- several matches to acidobacteria clones - two matches on the same clone, adjacent paralog? - matches to Solibacter and Ellin345 - environment conserved? - to be verified 
LUAcid_KM3_B8_172430325802  conserved hypothetical 1DADDED   - IPR006025 Peptidase M, neutral zinc metallopeptidases, zinc-binding sitePROTEIN  GoodABE82.227.62.18027/09/07 08:43:47- several matches to acidobacteria clones - one matche to each Solibacter and Ellin345 - environment conserved? - to be verified 
LUAcid_KM3_B8_162420123215  Alcohol dehydrogenase GroES domain protein 1RADDED   - IPR002085 family Alcohol dehydrogenase superfamily, zinc-containing - best match Archaea - best match to Alcohol dehydrogenase GroES domain protein Candidatus Nitrosopumilus maritimus SCM1 (50% id) - GroES (chaperonin 10) is an oligomeric molecular chaperone, which functions in protein folding and possibly in intercellular signalling, being found on the surface of various prokaryotic and eukaryotic cells, as well as being released from cells - high of domains found also in EPROTEINCOG1063 Threonine dehydrogenase and related Zn-dependent dehydrogenasesE Amino acid transport and metabolismGoodAB82.227.62.18027/09/07 09:24:14- one match in KM3_205_D9 (64% id) - several matches in acidobacterial genimes - environment conserved? - to be verified 
LUAcid_KM3_B8_23SRNA1898621859  hypothetical protein 1DADDED   - no COG - only one single match: Ellin345_1727 (54% id) - unique AcidobacteriaPROTEIN   unique82.227.62.18027/09/07 09:28:44- several matches in acidobacterial clones - one match in Ellin345, not in Solibacter - environment conserved? - to be verified 
LUAcid_KM3_B8_141522516442  tyrosyl-tRNA synthetase (TyrS) 1DADDED 6.1.1.1 - best match Ellin345 (62% id) - also found in A and EPROTEINCOG0162 Tyrosyl-tRNA synthetaseJ Translation, ribosomal structure and biogenesisGoodB82.227.62.18027/09/07 09:36:41- environment conserved in Acidobacteria - also in soil clones - to be verified 
LUAcid_KM3_B8_131456515179  phosphoribosylglycinamide formyltransferase (PurN) 1DADDED 2.1.2.2 - also COG0788 - purin biosynthesis operonPROTEINCOG0299 Folate-dependent phosphoribosylglycinamide formyltransferase PurNF Nucleotide transport and metabolismGoodABE82.227.62.18027/09/07 09:40:16- environment conserved in Acidobacteria - also in soil clones - to be verified 
LUAcid_KM3_B8_121348714536  phosphoribosylformylglycinamidine cyclo ligase (PurM) 1DADDED 6.3.3.1 - also COG0788 - purin biosynthesis operon - also present in A and EPROTEINCOG0150 Phosphoribosylaminoimidazole (AIR) synthetaseF Nucleotide transport and metabolismGoodB82.227.62.18027/09/07 09:42:54- environment conserved in Acidobacteria - also in soil clones - to be verified 
LUAcid_KM3_B8_111227613463  hypothetical protein 1DADDED   - no COG1413 low match - IPR011989 Armadillo-like helical - length and matches only AcidobacteriaPROTEINCOG1413 FOG: HEAT repeatC Energy production and conversionGoodunique82.227.62.18027/09/07 09:47:41- environment conserved in Acidobacteria - also in soil clones - to be verified 
LUAcid_KM3_B8_101081912276  amidophosphoribosyl transferase (PurF) 1DADDED 2.4.2.14 - purin biosynthesis operonPROTEINCOG0034 Glutamine phosphoribosylpyrophosphate amidotransferaseF Nucleotide transport and metabolismGoodBE82.227.62.18027/09/07 09:54:04- environment conserved in Acidobacteria - also in soil clones - to be verified 
LUAcid_KM3_B8_09859910815  putative phosphoribosylformylglycinamidine synthase II (truncated) 1DADDED 6.3.5.3 - purin biosynthesis operon - PurL ou PurM?PROTEINCOG0046 Phosphoribosylformylglycinamidine (FGAM) synthase, synthetase domainF Nucleotide transport and metabolismGoodAB82.227.62.18027/09/07 09:59:39- environment conserved in Acidobacteria - also in soil clones - to be verified 
LUAcid_KM3_B8_0878048514  phosphoribosylformylglycinamidine synthase I (PurQ) 1DADDED 6.3.5.3 - purin biosynthesis operon - IPR010075 Phosphoribosylformylglycinamidine synthase I - In some species, phosphoribosylformylglycinamidine synthase is composed of a single polypeptide chain. This entry describes the PurQ protein of Bacillus subtilis (where PurL, PurQ, and PurS are required for phosphoribosylformylglycinamidine synthase activity) and functionally equivalent proteins from other bacteria and archaea. PROTEINCOG0047 Phosphoribosylformylglycinamidine (FGAM) synthase, glutamine amidotransferase domainF Nucleotide transport and metabolismGoodAB82.227.62.18027/09/07 10:04:16- environment conserved in Acidobacteria - also in soil clones - to be verified 
LUAcid_KM3_B8_0775687804  phosphoribosylformylglycinamidine synthase I (PurS component) 1DADDED 6.3.5.3 - purin biosynthesis operon IPR003850 Phosphoribosylformylglycinamidine synthetase PurS Therefore, the PurS protein appears to be required for the function of the PurL and PurQ subunits of the FGAM synthetase, but the molecular mechanism for the functional role of PurS is currently not knownPROTEINCOG1828 Phosphoribosylformylglycinamidine (FGAM) synthase, PurS componentF Nucleotide transport and metabolism-AB82.227.62.18027/09/07 10:12:08- environment conserved in Acidobacteria - also in soil clones - to be verified 
LUAcid_KM3_B8_0662587568  adenylosuccinate lyase (PurB) 1DADDED 4.3.2.2 - purin biosynthesis operon - IPR004769 Adenylosuccinate lyasePROTEINCOG0015 Adenylosuccinate lyaseF Nucleotide transport and metabolismGoodAB82.227.62.18027/09/07 10:14:18- environment conserved in Acidobacteria - also in soil clones - to be verified 
LUAcid_KM3_B8_0556146231  beta-lactamase-like protein 1DADDED   - matches too short N-terminal - COG domain N-terminal truncated - only match corresponding to length: KM4_9_H7_148 - also COG1235: Metal-dependent hydrolases of the beta-lactamase superfamily I - good match to APROTEINCOG1235 Metal-dependent hydrolases of the beta-lactamase superfamily IR General function prediction onlyGood 82.227.62.18027/09/07 10:26:17- matches in acidobacterial clones - environment conserved in Acidobacteria? - to be verified 
LUAcid_KM3_B8_0440975353  peptidase M16 domain protein 1DADDED    PROTEINCOG0612 Predicted Zn-dependent peptidasesR General function prediction onlyGood 82.227.62.18027/09/07 10:29:32- matches to acidobacterial clones - to be verified - near to purin biosynthesis operon? 
LUAcid_KM3_B8_0339112835  radical SAM enzyme, Cfr family 1RADDED   - IPR004383 Conserved hypothetical protein 48 - family affiliated to BacteriaPROTEINCOG0820 Predicted Fe-S-cluster redox enzymeR General function prediction onlyGoodB82.227.62.18027/09/07 10:33:07- matches to acidobacterial clones - to be verified - near to purin biosynthesis operon? - near to purin operon? 
LUAcid_KM3_B8_0215582832  MiaB-like tRNA modifying enzyme 1DADDED   - also IPR005839 Protein of unknown function UPF0004 - IPR006467 MiaB-like tRNA modifying enzymePROTEINCOG0621 2-methylthioadenine synthetaseJ Translation, ribosomal structure and biogenesisGoodB82.227.62.18027/09/07 10:35:58- matches to acidobacterial clones - to be verified - near to purin biosynthesis operon? - near to purin operon? 
LUAcid_KM3_B8_011360644  Na+/H+ antiporter NhaC 1RADDED   - IPR004770 Na+/H+ antiporter NhaC - also some in APROTEINCOG1757 Na+/H+ antiporterC Energy production and conversionGoodB82.227.62.18027/09/07 10:38:06- matches in acidobacterial clones G4 and D2; match in Solibacter, not in Acid Ellin6076 - environment conserved in acidobacteria - to be verified 
LUAcid_KM3_B8_152245523189  hypothetical protein 1DADDED   - no COGPROTEIN  Good 82.227.62.18027/09/07 10:40:18- one match to Acid345_1727 - environment conserved in acidobacterial clones - to be verified 
LUAcid_KM3_B8_354335443605  hypothetical partial holliday junction DNA helicase RuvB 1DADDED   - N-terminal RuvB sequence; approximately 25% of the lengthPROTEIN  - 82.227.62.18027/09/07 11:04:07  
LUAcid_KM3_B8_16SRNA1673818301  16S ribosomal RNA 1DADDED16SrRNA   RNA    129.175.105.7529/10/07 18:10:03  
LUAcid_KM3_B8_23SRNA1898621859  23S ribosomal RNA 1DADDED23SrRNA   RNA    129.175.105.7529/10/07 18:10:32  
LUAcid_KM3_B8_5SRNA2214422270  5S ribosomal RNA 1DADDED5SrRNA   RNA    129.175.105.7529/10/07 18:11:11  
LUAcid_KM3_B8_TRNA11845118524  tRNA-Ile (GAT) 1DADDEDtRNA-Ile   TRNA    129.175.105.7529/10/07 18:14:12  
LUAcid_KM3_B8_TRNA21871818790  tRNA-Ala (TGC) 1DADDEDtRNA-Ala   TRNA    129.175.105.7529/10/07 18:16:36  
LUAcid_KM3_B8_354335443605  hypothetical partial holliday junction DNA helicase RuvB 1DADDED   - N-terminal RuvB sequence; approximately 25% of the lengthPROTEINCOG2255 Holliday junction resolvasome, helicase subunitL Replication, recombination and repair- 129.175.105.7517/12/07 16:31:46