Current Annotation for L35516_KM4_9_H7 23/11/17

namestartcoordendcoord**deintronstart1directionstatusgnecgpcctype1kognumkogglimmerdompatauth10/10/03 00:00:00More Comments 
                    10/10/03 00:00:00  
LUAcid_KM4_H7_242009620770  beta-lactamase-like protein 1DADDED   - protein N-terminal too short (60aa?) - best match euryarchaeote: eta-lactamase domain protein, Candidatus Methanoregula boonei 6A8 (35% id), then bacteria - also COG0324 - seems to be part of purin operonPROTEINCOG1235 Metal-dependent hydrolases of the beta-lactamase superfamily IR General function prediction onlyGoodBE82.227.62.18018/09/07 10:59:17- paralogs on different clones LUAcid_KM3_G1_01 LUAcid_KM3_D9_13 LUAcid_AD106_G4_08 L35498_AD55_D2_360 LUAcid_KM4_H7_24 
LUAcid_KM4_H7_252084422154  adenylosuccinate lyase (PurB) 1DADDED   - best match to AD55_D2_353 (70% id) - next match 23k22_16 (70% id) - purine ribonucleotide biosynthetic process PROTEINCOG0015 Adenylosuccinate lyaseF Nucleotide transport and metabolismGoodABE82.227.62.18018/09/07 11:33:11- orthologs found in other clones: LUAcid_KM3_G1_02 LUAcid_KM3_D9_14 L35498_AD55_D2_353 LUAcid_AD106_G4_09 LUAcid_KM4_H7_25 23k22_016 PurB (AY281356 soil clone) 41b15_012 PurB (AY281352 soil clone)  
LUAcid_KM4_H7_262220922472  phosphoribosylformylglycinamidine synthase I (PurS) 1DADDED   - best match to 23k22_011 PurS soil clone AY281352 (73% id) - purin biosynthesis pathwayPROTEINCOG1828 Phosphoribosylformylglycinamidine (FGAM) synthase, PurS componentF Nucleotide transport and metabolismGoodAB82.227.62.18018/09/07 11:48:26- orthologs on other acidobacterial clones: LUAcid_KM3_G1_03 L35498_AD55_D2_340 LUAcid_KM3_D9_15 LUAcid_AD106_G4_10 LUAcid_KM4_H7_26 23k22_011 PurS AY281352 41b15_015 PurS AY281356 
LUAcid_KM4_H7_272253323240  phosphoribosylformylglycinamidine synthase I (PurQ) 1DADDED   best match to KM3_205_D9_262 (84% id) - Purin biosynthesis operonPROTEINCOG0047 Phosphoribosylformylglycinamidine (FGAM) synthase, glutamine amidotransferase domainF Nucleotide transport and metabolismGoodAB82.227.62.18018/09/07 13:10:00- several orthologs in Acidobacteria LUAcid_KM3_G1_04 LUAcid_KM3_D9_16 L35498_AD55_D2_336 LUAcid_AD106_G4_11 LUAcid_KM4_H7_27 41b15_014 PurQ AY281356 23k22_010 PurQ AY281352 Acid345_2235 Acid_5943 
LUAcid_KM4_H7_282326525478  phosphoribosylformylglycinamidine synthase II (PurL) 1DADDED 6.3.5.3 best match to 23k22_009 PurL AY281352 (66% id) - purin biosynthesis operon - AIR synthase related protein - ’de novo’ IMP biosynthetic process - hosphoribosylformylglycinamidine synthase is a single, long polypeptide in most bacteria. Three proteins are required in Bacillus subtilis and many other species. This is the longest of the three and is designated PurL, phosphoribosylformylglycinamidine synthase II, or FGAM synthase II.PROTEINCOG0046 Phosphoribosylformylglycinamidine (FGAM) synthase, synthetase domainF Nucleotide transport and metabolismGoodAB82.227.62.18018/09/07 14:01:29several orthologs in Acidobacteria: LUAcid_KM3_G1_06 LUAcid_KM3_D9_17 L35498_AD55_D2_328 LUAcid_AD106_G4_12 LUAcid_KM4_H7_28 L35511_KM3_47_B8_428 23k22_009 PurL AY281352 41b15_013 PurL AY281356 Acid_5944 Acid345_4622 
LUAcid_KM4_H7_292555126975  amidophosphoribosyl transferase (PurF) 1DADDED 2.4.2.14 - best match to 41b15_012 PurF AY281356 (71% id) - purin biosynthesis operon - also COG0449 - Purine nucleotides are synthesised both via the de novo pathway and via the salvage pathway and are vital for cell functions and cell proliferation through DNA and RNA syntheses and ATP energy supply. Amidophosphoribosyltransferase (EC:2.4.2.14) is the rate-limiting enzyme in the de novo pathway of purine ribonucleotide synthesis and is regulated by feedback inhibition by AMP and GMP [1].PROTEINCOG0034 Glutamine phosphoribosylpyrophosphate amidotransferaseF Nucleotide transport and metabolismGoodABE82.227.62.18018/09/07 16:25:16several othologs in Acidobacteria LUAcid_KM3_G1_07 LUAcid_KM3_D9_18 L35511_KM3_47_B8_405 L35498_AD55_D2_302 LUAcid_AD106_G4_13 LUAcid_KM4_H7_29 41b15_012 PurF AY281356 23k22_008 PurF AY281352 Acid345_4621 Acid_5945 
LUAcid_KM4_H7_191413615416  phosphoribosylamineglycine ligase (PurD) 1DADDED   - best match to 23k22_017 (58% id)PROTEINCOG0151 Phosphoribosylamine-glycine ligaseF Nucleotide transport and metabolismGoodABE82.227.62.18018/09/07 17:02:29present on soil clones and one copy in both genomes: LUAcid_KM4_H7_19 23k22_017 PurD AY281352 41b15_020 PurD AY281356 Acid345_0231 Acid_0014 
LUAcid_KM4_H7_201547215972  phosphoribosylaminoimidazole carboxylase (PurE) 1DADDED   - best match to 23k22_016 PurE (62% id) - Purin biosynthesis operon - synonyme Phosphoribosylcarboxyaminoimidazole (NCAIR) mutase - present also in E but affiliation ABPROTEINCOG0041 Phosphoribosylcarboxyaminoimidazole (NCAIR) mutaseF Nucleotide transport and metabolismGoodAB82.227.62.18018/09/07 17:09:29- one match in both genomes - match to 23k22_016 PurE ;AY281352 - absent in 41b15 
LUAcid_KM4_H7_181395713757  30S Ribosomal protein S21 1RADDED   - best match to 30S ribosomal protein 23k22_018 AY281352 (?id) - IPR001911 Ribosomal protein S21PROTEIN  -B82.227.62.18018/09/07 17:15:46- ortholog in 23k22_018 AY281352 
LUAcid_KM4_H7_171352812641  prenyltransferase-like protein 1RADDED   - best match to 23k22_022 AY281352 (56% id) - no COG - Molecular Function:prenyltransferase activity - IPR000537 UbiA prenyltransferase - membrane protein - UbiA prenyltransferase domainPROTEIN  GoodABE82.227.62.18018/09/07 17:27:16ortholog to soil clone proteins: 23k22_022 AY281352 (56% id) 41b15_023 AY281356 
LUAcid_KM4_H7_161262511498  protein of unknown function DUF362 1RADDED   - best match to hypothetical protein Mbar_A1412 Methanosarcina barkeri str. Fusaro (50% id) - no COGPROTEIN  GoodAB82.227.62.18018/09/07 18:10:59no match in both acidobacterial genomes - strong match to 23k22_023 AY281352 (43% id), not to 41b15 
LUAcid_KM4_H7_151143110586  hypothetical protein 1RADDED   - best match to 41b15_024 AY281356 (38% id) - no COG - no domainPROTEIN  Goodunique82.227.62.18018/09/07 18:14:09- match to 41b15_024 AY281356 (38% id) 
LUAcid_KM4_H7_12100789428  2OG-Fe(II) oxygenase like protein 1RADDED   - InterPro relationship to Alkylated DNA repair protein AlkB - not in ArchaeaPROTEINCOG3145 Alkylated DNA repair proteinL Replication, recombination and repair-AE82.227.62.18018/09/07 18:27:48match to one ORF in the both acidobacterial genomes; no match in clones 
LUAcid_KM4_H7_1193908884  conserved hypothetical protein 1RADDED   - best match to Burkholderia phymatum STM815 (42%) - no COG - low phospholipid domain (pfam04972) - in B and EPROTEIN  Good but overlapping 82.227.62.18018/09/07 18:33:23- no match to Acidobacteria 
LUAcid_KM4_H7_1085748891  antibiotic biosynthesis monooxygenase family enzyme 1DADDED   - best match to Rhodoferax ferrireducens T118 (76% id) - only 7 matches: Rhodoferax, Alcanivorax, Marinobacter algicola DG893, Idiomarina, Marinobacter sp. ELB17, Marinobacter aquaeolei, marine gamma proteobacterium HTCC2080 - conserved ony in a small group of Bacteria: beta, gamma protobacteria and here AcidobacteriaPROTEIN  - 82.227.62.18018/09/07 18:43:56- no match to Acidobacteria 
LUAcid_KM4_H7_1085748891  antibiotic biosynthesis monooxygenase family enzyme 1DADDED   - best match to Rhodoferax ferrireducens T118 (76% id) - only 7 matches: Rhodoferax, Alcanivorax, Marinobacter algicola DG893, Idiomarina, Marinobacter sp. ELB17, Marinobacter aquaeolei, marine gamma proteobacterium HTCC2080 - conserved ony in a small group of Bacteria: beta, gamma protobacteria and here Acidobacteria - This domain is found in monooxygenases involved in the biosynthesis of several antibiotics by Streptomyces species, which can carry out oxygenation without the assistance of any of the prosthetic groups, metal ions or cofactors normally associated with activation of molecular oxygen.PROTEIN  - 82.227.62.18018/09/07 18:47:51- no match to Acidobacteria 
LUAcid_KM4_H7_0978948514  conserved hypothetical protein 1DADDED   - best match to Rhodoferax ferrireducens T118 (65% id) - present in a relatively small number of Bacteria, i.e. Rhodoferax, Burkholderia, Polaromonas naphthalenivorans... - no COG - interPro- no domainPROTEIN  - 82.227.62.18018/09/07 18:58:47- no match to Acidobacteria - environment to be verified 
LUAcid_KM4_H7_0877817185  NADPH-dependent FMN reductase 1RADDED 1.5.1.29 - very strong match to Polaromonas naphthalenivorans CJ2 (87% id) - very conserved protein - orthologs also in AEB, but affiliation to B PROTEINCOG0431 Predicted flavoproteinR General function prediction onlyGood but overlappingAB82.227.62.18019/09/07 09:25:05- low matches in both acidobacterial genomes - not in clones - environment conserved at least in Paloromonas --> to be analysed 
LUAcid_KM4_H7_0764607104  acyl carrier protein phosphodiesterase 1DADDED   - very high similarity to Polaromonas naphthalenivorans CJ2 (85% id) - flavodoxin-like fold domaine - occurence also iin A and EPROTEINCOG1182 Acyl carrier protein phosphodiesteraseI Lipid transport and metabolism-B82.227.62.18019/09/07 09:34:22- no matches in Acidobacteria - environment conserved with previous protein in Paloromonas 
LUAcid_KM4_H7_0662325330  transcriptional regulator, LysR family 1RADDED   - high similarity to Polaromonas naphthalenivorans CJ2 (76% id) - Numerous bacterial transcription regulatory proteins bind DNA via a helix-turn-helix (HTH) motif -IPR000847 Bacterial regulatory protein, LysRPROTEINCOG0583 Transcriptional regulatorK Transcription-B82.227.62.18019/09/07 09:40:25- low matches in both genomes - not in other clones - environment conserved in Polaromonas 
LUAcid_KM4_H7_0551044091  Thiosulphate-binding protein 1RADDED   - very similar to Polaromonas sp. JS666 (85% id)PROTEINCOG1613 ABC-type sulfate transport system, periplasmic componentP Inorganic ion transport and metabolism-B82.227.62.18019/09/07 09:54:17- no match in Acidobacteria - enironment conserved in Burkholderia; to be verified - part of operon like organisation in Bacteria 
LUAcid_KM4_H7_0440843209  sulfate ABC transporter, inner membrane subunit CysT 1RADDED   - high similarity to Polaromonas sp. JS666 (85% id) - highly conserved protein in B - Binding-protein-dependent transport systems inner membrane component - large group of transporter in A, B, and E - sulfate transmembrane transporter activity - integral membrane protein - COG affiliation sounds strange?PROTEINCOG0555 ABC-type sulfate transport system, permease componentO Posttranslational modification, protein turnover, chaperonesGood but overlappingB82.227.62.18019/09/07 10:01:23- paralog to adjacent protein on KM4_9_H7_323 and 330 - lower matches in acidobacterial genomes 
LUAcid_KM4_H7_0332092301  sulfate ABC transporter, inner membrane subunit CysW 1RADDED   - high similarity to Polaromonas sp. JS666 (74% id) - second subunit of sulfate transporterPROTEINCOG4208 ABC-type sulfate transport system, permease componentP Inorganic ion transport and metabolism-B82.227.62.18019/09/07 10:05:44- similarity to adjacent protein; two different subunits - environment conserved? 
LUAcid_KM4_H7_0440843209  sulfate ABC transporter, inner membrane subunit CysT 1RADDED   - high similarity to Polaromonas sp. JS666 (85% id) - highly conserved protein in B - Binding-protein-dependent transport systems inner membrane component - large group of transporter in A, B, and E - sulfate transmembrane transporter activity - integral membrane protein - COG affiliation sounds strange?PROTEINCOG0555 ABC-type sulfate transport system, permease componentO Posttranslational modification, protein turnover, chaperonesGood but overlappingB82.227.62.18019/09/07 10:07:17- similarity to adjacent protein on KM4_9_H7_323 (CysT su) and 330 (CysW su) - lower matches in acidobacterial genomes 
LUAcid_KM4_H7_0222741192  sulfate ABC transporter, ATPase subunit 37RADDED   - very similar to Polaromonas naphthalenivorans CJ2 (80% id) - highly conserved in BPROTEINCOG1118 ABC-type sulfate/molybdate transport systems, ATPase componentP Inorganic ion transport and metabolismGood but overlappingB82.227.62.18019/09/07 10:11:12- several matches in both acidobacterial genomes - operon-like organisation? 
LUAcid_KM4_H7_01950432  Disulphide bond formation protein DsbB 28RADDED   - high similarity to Polaromonas sp. JS666 (72% id) - disulphide oxidoreductase - conserved in B - group of protein affiliated to proteobacteriaPROTEINCOG1495 Disulfide bond formation protein DsbBO Posttranslational modification, protein turnover, chaperonesGoodB82.227.62.18019/09/07 10:18:40- no matches in Acidobacteria - environment conserved in Brukholderia? 
LUAcid_KM4_H7_141058610272  truncated hexosyltransferase 1RADDED   - only N-terminal matches to hexosyltransgferases (approx. 80aa)PROTEIN  - 82.227.62.18019/09/07 11:34:27- N-terminal match to 41b15_025 AY281356 putative hexosyltransferase 
LUAcid_KM4_H7_131037510133  hypothetical protein 1RADDED   - C-terminal best match to Polaromonas (91% id C-terminal, 70aa); truncated of 160aaPROTEIN  - 82.227.62.18019/09/07 11:42:35C-terminal good matches to conserved hypothetical proteins in both acidobacterial genomes (Acid_4008) (78% id in the matched region of 70aa) - C-terminal COG3826 - to be revised 
LUAcid_KM4_H7_302709928127  phosphoribosylformylglycinamidine cyclo ligase (PurM) 1DADDED   - IPR000728 AIR synthase related protein - purin biosynthesis operonPROTEINCOG0150 Phosphoribosylaminoimidazole (AIR) synthetaseF Nucleotide transport and metabolismGood 82.227.62.18021/09/07 10:28:18- environment conserved in Acidobacteria - to be verified 
LUAcid_KM4_H7_312812728765  phosphoribosylglycinamide formyltransferase (PurN) 1DADDED   - purin biosynthesis operonPROTEINCOG0299 Folate-dependent phosphoribosylglycinamide formyltransferase PurNF Nucleotide transport and metabolismGood 82.227.62.18021/09/07 10:29:44- environment conserved in Acidobacteria 
LUAcid_KM4_H7_322962928781  conserved hypothetical protein 1RADDED   PR004843 Metallophosphoesterase domainPROTEINCOG1408 Predicted phosphohydrolasesR General function prediction onlyGood 82.227.62.18021/09/07 10:32:34- environment conserved in Acidobacteria - to be verified - match to soil clone 23k22_002 
LUAcid_KM4_H7_332969430941  tyrosyl-tRNA synthetase 1DADDED 6.1.1.1  PROTEINCOG0162 Tyrosyl-tRNA synthetaseJ Translation, ribosomal structure and biogenesisGoodABE82.227.62.18021/09/07 10:34:06- environment conserved in Acidobacteria - to be verified 
LUAcid_KM4_H7_231858119897  peptidase M16 domain protein 1DADDED   - adjacent to purin biosynthesis operon - IPR001431 Peptidase M16, zinc-binding site - not in APROTEINCOG0612 Predicted Zn-dependent peptidasesR General function prediction onlyGoodB82.227.62.18001/10/07 11:33:01- several matches in acidobacterial clones (soil and marine) - environment conserved? - to be verified 
LUAcid_KM4_H7_221839417357  radical SAM enzyme, Cfr family 1RADDED   - adjacent to purin biosynthesis operon - IPR004383 Conserved hypothetical protein 48 - not in A - not in APROTEINCOG0820 Predicted Fe-S-cluster redox enzymeR General function prediction onlyGoodB82.227.62.18001/10/07 11:38:55- several matches in acidobacterial clones (soil and marine) - environment conserved? - to be verified 
LUAcid_KM4_H7_211597217252  MiaB-like tRNA modifying enzyme 1DADDED   - adjacent to purin biosynthesis operon - IPR005839 Protein of unknown function UPF0004 - Elongator protein 3/MiaB/NifB - Radical SAM domain - not in APROTEINCOG0621 2-methylthioadenine synthetaseJ Translation, ribosomal structure and biogenesisGoodABE82.227.62.18001/10/07 11:41:28- several matches in acidobacterial clones (soil and marine) - environment conserved? - to be verified 
LUACID_KM4_H7_322962928781  Metallophosphoesterase 1RADDED   PR004843 Metallophosphoesterase domainPROTEINCOG1408 Predicted phosphohydrolasesR General function prediction onlyGood 82.227.62.18009/10/07 09:50:05- environment conserved in Acidobacteria - to be verified - match to soil clone 23k22_002 
LUACID_KM4_H7_0764607104  acyl carrier protein phosphodiesterase 1DADDED   - very high similarity to Polaromonas naphthalenivorans CJ2 (85% id) - flavodoxin-like fold domaine - occurence also iin A and E - could be an Azoreductase involved in Mordant yellow 3 pathway (industrial dye biodegradationPROTEINCOG1182 Acyl carrier protein phosphodiesteraseI Lipid transport and metabolism-B (only bacteria)82.227.62.18009/10/07 11:22:06- no matches in Acidobacteria - environment conserved with previous protein in Paloromonas 
LUACID_KM4_H7_16SRNA3124232803  16S ribosomal RNA 1DADDED16SrRNA   RNA  - 129.175.105.7529/10/07 18:30:27  
LUACID_KM4_H7_23SRNA3340334997  23S ribosomal RNA 1DADDED23SrRNA   RNA    129.175.105.7529/10/07 18:31:02  
LUACID_KM4_H7_TRNA13305833131  tRNA-Ile (GAT) 1DADDEDtRNA-Ile   TRNA    129.175.105.7529/10/07 18:32:00  
LUACID_KM4_H7_TRNA23314833220  tRNA-Ala (TGC) 1RADDEDtRNA-Ala   TRNA    129.175.105.7529/10/07 18:32:33  
LUACID_KM4_H7_171352812641  prenyltransferase-like protein 1RADDED   - best match to 23k22_022 AY281352 (56% id) - no COG - Molecular Function:prenyltransferase activity - IPR000537 UbiA prenyltransferase - membrane protein - UbiA prenyltransferase domainPROTEINCOG0382 4-hydroxybenzoate polyprenyltransferase and related prenyltransferasesH Coenzyme transport and metabolismGoodABE129.175.105.7517/12/07 16:41:35ortholog to soil clone proteins: 23k22_022 AY281352 (56% id) 41b15_023 AY281356 fixed COG -yz 
LUACID_KM4_H7_131037510133  hypothetical protein 1RADDED   - C-terminal best match to Polaromonas (91% id C-terminal, 70aa); truncated of 160aaPROTEINCOG3826 Uncharacterized protein conserved in bacteriaS Function unknown- 129.175.105.7517/12/07 17:26:00fixed COG -yz 
LUACID_KM4_H7_141058610272  truncated hexosyltransferase 1RADDED   - only N-terminal matches to hexosyltransgferases (approx. 80aa)PROTEINCOG0038 Chloride channel protein EriCP Inorganic ion transport and metabolism- 129.175.105.7517/12/07 17:31:23- N-terminal match to 41b15_025 AY281356 putative hexosyltransferase fixed COG -yz