ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI0048 good R KOG2960 General function prediction only Protein involved in thiamine biosynthesis and DNA damage tolerance
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI0048 15211 14240 -324
(324 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGR144w [R] KOG2960 Protein involved in thiamine biosynthesis an... 480 e-135
SPBC26H8.01 [R] KOG2960 Protein involved in thiamine biosynthesi... 338 6e-93
At5g54770 [R] KOG2960 Protein involved in thiamine biosynthesis ... 293 2e-79
>YGR144w [R] KOG2960 Protein involved in thiamine biosynthesis and DNA
damage tolerance
Length = 326
Score = 480 bits (1236), Expect = e-135
Identities = 243/327 (74%), Positives = 274/327 (83%), Gaps = 4/327 (1%)
Query: 1 MSTTQTQTQ---NKLSVQSQSVEHCLSDVVKQDDWSDFAFAPIREATVSRAMTSRYFKDL 57
MS T T T ++L + S V HCLSD+VK++DWSDF FAPIRE+TVSRAMTSRYFKDL
Sbjct: 1 MSATSTATSTSASQLHLNSTPVTHCLSDIVKKEDWSDFKFAPIRESTVSRAMTSRYFKDL 60
Query: 58 DKFAVSDVVIIGAGSSGLSAAYVIAKNRPDLKIAIIEANVAPGGGCWLGGQLFSAMIMRK 117
DKFAVSDV+I+GAGSSGLSAAYVIAKNRPDLK+ IIE++VAPGGG WLGGQLFSAM+MRK
Sbjct: 61 DKFAVSDVIIVGAGSSGLSAAYVIAKNRPDLKVCIIESSVAPGGGSWLGGQLFSAMVMRK 120
Query: 118 PADKFLDELNIPYEDEGHYVVVKHAALFMSTVLSEVLKFPNVKMFNATAVEDLVTRPADD 177
PA FL EL IPYEDEG YVVVKHAALF+STVLS+VL+ PNVK+FNAT VEDLVTRP +
Sbjct: 121 PAHLFLQELEIPYEDEGDYVVVKHAALFISTVLSKVLQLPNVKLFNATCVEDLVTRPPTE 180
Query: 178 NSQHVNVAGVVTNWTLVTQNHDTQSCMDPNVIELSGYKDNGERDLSQKHGVILSCCGHDG 237
+ V VAGVVTNWTLVTQ H TQ CMDPNVIEL+GYK++G RDLSQKHGVILS GHDG
Sbjct: 181 KGE-VTVAGVVTNWTLVTQAHGTQCCMDPNVIELAGYKNDGTRDLSQKHGVILSTTGHDG 239
Query: 238 PFGAFTVKRMASIDSSKSYAGMKGLDMNRAEDAVVKNAGAYDKVGSVYFAGMEVAEHAGL 297
PFGAF KR+ ID ++ GMKGLDMN AE VV ++GAY V ++YFAGMEVAE GL
Sbjct: 240 PFGAFCAKRIVDIDQNQKLGGMKGLDMNHAEHDVVIHSGAYAGVDNMYFAGMEVAELDGL 299
Query: 298 NRMGPTFGAMAVSGIKAAEDILKHFAA 324
NRMGPTFGAMA+SG+ AAE ILKHFAA
Sbjct: 300 NRMGPTFGAMALSGVHAAEQILKHFAA 326
>SPBC26H8.01 [R] KOG2960 Protein involved in thiamine biosynthesis and DNA
damage tolerance
Length = 328
Score = 338 bits (867), Expect = 6e-93
Identities = 175/288 (60%), Positives = 208/288 (71%), Gaps = 19/288 (6%)
Query: 35 FAFAPIREATVSRAMTSRYFKDLDKFAVSDVVIIGAGSSGLSAAYVIAKNRPDLKIAIIE 94
F+FAPIRE+TVSRAMT RYF DLDK+A SD+VI+GAGS+GL+AAY I RPDLKIAIIE
Sbjct: 49 FSFAPIRESTVSRAMTRRYFSDLDKYAESDIVIVGAGSAGLTAAYYIGTRRPDLKIAIIE 108
Query: 95 ANVAPGGGCWLGGQLFSAMIMRKPADKFLDELNIPYEDEGHYVVVKHAALFMSTVLSEVL 154
A+VAPGGG WLGGQLFSAM++RKPAD FL+E+ +PYEDEG YVVVKHAALF STV++ L
Sbjct: 109 ASVAPGGGAWLGGQLFSAMVVRKPADLFLNEIGVPYEDEGDYVVVKHAALFTSTVMARTL 168
Query: 155 KFPNVKMFNATAVEDLVTRPADDNSQHVNVAGVVTNWTLVTQNHDTQSCMDPNVIELSGY 214
PNVK+FNATAVEDL+ + D Q +AGVVTNWTLV+ NH QSCMDPN I
Sbjct: 169 ALPNVKLFNATAVEDLIVKEGKDGKQ--RIAGVVTNWTLVSLNHGLQSCMDPNTINAH-- 224
Query: 215 KDNGERDLSQKHGVILSCCGHDGPFGAFTVKRMASIDSSKSYAGMKGLDMNRAEDAVVKN 274
+++S GHDGPFGAF VKR+AS + M+ LDMNRAED +VK
Sbjct: 225 -------------LVVSATGHDGPFGAFCVKRLASAQLVSNLHDMRPLDMNRAEDLIVK- 270
Query: 275 AGAYDKVGSVYFAGMEVAEHAGLNRMGPTFGAMAVSGIKAAEDILKHF 322
G + + GME++E G NRMGPTFG M SGIKAA++ L F
Sbjct: 271 -GTREVFPGMIVGGMELSEFDGANRMGPTFGGMMFSGIKAAQEALAIF 317
>At5g54770 [R] KOG2960 Protein involved in thiamine biosynthesis and DNA
damage tolerance
Length = 349
Score = 293 bits (751), Expect = 2e-79
Identities = 158/290 (54%), Positives = 198/290 (67%), Gaps = 25/290 (8%)
Query: 31 DWSDFAFAPIREATVSRAMTSRYFKDLDKFAVSDVVIIGAGSSGLSAAYVIAKNRPDLKI 90
D + F F PI+E+ VSR MT RY D+ +A +DVV++GAGS+GLSAAY I+KN P++++
Sbjct: 52 DLNAFTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKN-PNVQV 110
Query: 91 AIIEANVAPGGGCWLGGQLFSAMIMRKPADKFLDELNIPYEDEGHYVVVKHAALFMSTVL 150
AIIE +V+PGGG WLGGQLFSAMI+RKPA FLDE+ + Y+++ YVVVKHAALF ST++
Sbjct: 111 AIIEQSVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIM 170
Query: 151 SEVLKFPNVKMFNATAVEDLVTRPADDNSQHVNVAGVVTNWTLVTQNHDTQSCMDPNVIE 210
S++L PNVK+FNA A EDL+ + V GVVTNW LV QNH TQSCMDPNV+E
Sbjct: 171 SKLLARPNVKLFNAVAAEDLIVKGN-------RVGGVVTNWALVAQNHHTQSCMDPNVME 223
Query: 211 LSGYKDNGERDLSQKHGVILSCCGHDGPFGAFTVKRMASIDSSKSYAGMKGLDMNRAEDA 270
+++S CGHDGPFGA VKR+ SI GMK LDMN AEDA
Sbjct: 224 AK---------------IVVSSCGHDGPFGATGVKRLKSIGMIDHVPGMKALDMNTAEDA 268
Query: 271 VVKNAGAYDKVGSVYFAGMEVAEHAGLNRMGPTFGAMAVSGIKAAEDILK 320
+V+ + V + GMEVAE G RMGPTFGAM +SG KA + LK
Sbjct: 269 IVRL--TREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAGQLALK 316
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.132 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,322,894
Number of Sequences: 60738
Number of extensions: 748763
Number of successful extensions: 1815
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1804
Number of HSP's gapped (non-prelim): 3
length of query: 324
length of database: 30,389,216
effective HSP length: 106
effective length of query: 218
effective length of database: 23,950,988
effective search space: 5221315384
effective search space used: 5221315384
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)