ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI0204 good K KOG2402 Transcription Paf1/RNA polymerase II complex, RTF1 component (involved in regulation of TATA box-binding protein)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI0204 72233 73978 582
(582 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGL244w [K] KOG2402 Paf1/RNA polymerase II complex RTF1 componen... 454 e-127
Hs14749361 [K] KOG2402 Paf1/RNA polymerase II complex RTF1 compo... 97 9e-20
SPBC651.09c [K] KOG2402 Paf1/RNA polymerase II complex RTF1 comp... 94 6e-19
At1g61040 [K] KOG2402 Paf1/RNA polymerase II complex RTF1 compon... 85 3e-16
7291410 [K] KOG2402 Paf1/RNA polymerase II complex RTF1 componen... 81 5e-15
CE09619 [K] KOG2402 Paf1/RNA polymerase II complex RTF1 componen... 70 9e-12
>YGL244w [K] KOG2402 Paf1/RNA polymerase II complex RTF1 component (involved
in regulation of TATA box-binding protein)
Length = 558
Score = 454 bits (1169), Expect = e-127
Identities = 248/496 (50%), Positives = 310/496 (62%), Gaps = 22/496 (4%)
Query: 89 PFPLEGKYKDEKDREKLELLPEMERESILFERSQQMQKYQERKLLRQHAKSIKQQQQLQR 148
PFPLEGKYKDE DRE LE LPEMERE++LFERSQ MQKYQERKL R + +K+QQQ +
Sbjct: 79 PFPLEGKYKDESDREHLESLPEMERETLLFERSQIMQKYQERKLFRARGRDMKEQQQRAK 138
Query: 149 --EGSKXXXXXXXXXXXXGHSDXXXXXXXXXXXXRAKKSGNYEFSDNXXXXXXXXXXXXX 206
E S+ GHSD RA+K+ +Y SDN
Sbjct: 139 NDEDSRKTRASTRSTHATGHSDIKASKLSQLKKQRARKNRHY--SDNEDEDDEEDYREED 196
Query: 207 XXXXXXXXXXXXXXXXXXXXXXXXXXLGYEKSKTRGRERADIHEVEWAED-DNLDRESSL 265
Y+K + EVEWAE+ D DRE +
Sbjct: 197 YKDDEGSEYGDDEEYNPFDRRDT-----YDKRE----------EVEWAEEEDEQDREPEI 241
Query: 266 DDYNKIQIGRSFVAKFCFYPHFNKIVEGCYGRVNIGKDKHTGNSMYRMVKIERVFLQKPY 325
D+NK++IGRSFVAKFCFYP F V+GCYGRVN+G DK TG + YRMV+IERVFLQKPY
Sbjct: 242 SDFNKLRIGRSFVAKFCFYPGFEDAVKGCYGRVNVGTDKRTGKTSYRMVRIERVFLQKPY 301
Query: 326 NMGKFFTNQYFGVTQGKDRKIFQMSYFSDSPVTQPEYERYLLSLEKYDITQPSPYILNNK 385
NMGKF+TNQYFGVTQGKDRK+FQM+YFSD + EY+RYL +L+ + +PS + L+NK
Sbjct: 302 NMGKFYTNQYFGVTQGKDRKVFQMNYFSDGLFAEDEYQRYLRALDNSQMIKPSLHSLSNK 361
Query: 386 SKAVNEFVCEPLTPKLMDDIVRNRMVFNKKLSGTNAVMEKQVLKEKLQFSKQSGDIESES 445
+K V +FV PLT K D++VR+RM FNKKLSGTNAV+EK VL+EKLQ++K++ + + +
Sbjct: 362 TKEVMDFVNTPLTDKTTDEVVRHRMQFNKKLSGTNAVLEKTVLREKLQYAKETNNEKDIA 421
Query: 446 KYANQLKALEKRLSSYEKHHENDQNDIKTLGALTSKNRKLNIDRIRHAETIKREEGVNKV 505
KY+ QL+ EKR+S YEKHHENDQ+DIK LG LTSKNRKLN+ IR+AE +K+E+ N
Sbjct: 422 KYSAQLRNFEKRMSVYEKHHENDQSDIKKLGELTSKNRKLNMSNIRNAEHVKKEDS-NNF 480
Query: 506 DAKSDPFSRLKTRTKXXXXXXXXXXXXXXXXXXXXQ-LEVDXXXXXXXXXXXXXXXFRRL 564
D+KSDPFSRLKTRTK + L+ D FRRL
Sbjct: 481 DSKSDPFSRLKTRTKVYYQEIQKEENAKAKEIAQQEKLQEDKDAKDKREKELLVAQFRRL 540
Query: 565 GGLEEIIGSLDLKLHI 580
GGLE ++G LD+K +
Sbjct: 541 GGLERMVGELDIKFDL 556
>Hs14749361 [K] KOG2402 Paf1/RNA polymerase II complex RTF1 component (involved
in regulation of TATA box-binding protein)
Length = 585
Score = 96.7 bits (239), Expect = 9e-20
Identities = 92/425 (21%), Positives = 177/425 (41%), Gaps = 25/425 (5%)
Query: 98 DEKDREKLELLPEMERESILFERSQQMQ----KYQERKLLRQHAKSIKQQ--QQLQREGS 151
DE+DR +LE + E ERE LF R ++ + +++ +K L+ K K++ ++ + E
Sbjct: 64 DEEDRARLEQMTEKEREQELFNRIEKREVLKRRFEIKKKLKTAKKKEKKEKKKKQEEEQE 123
Query: 152 KXXXXXXXXXXXXGHSDXXXXXXXXXXXXRAKKSGNYEFSDNXXXXXXXXXXXXXXXXXX 211
K H+ R +K +
Sbjct: 124 KKKLTQIQESQVTSHNKERRSK-------RDEKLDKKSQAMEELKAEREKRKNRTAELLA 176
Query: 212 XXXXXXXXXXXXXXXXXXXXXLGYEKSKTRGRERADIHEVEWAEDDNLDRESSL-DDYNK 270
EKS R + E E E + SL ++ N+
Sbjct: 177 KKQPLKTSEVYSDDEEEEEDDKSSEKSDRSSRTSSSDEEEEKEEIPPKSQPVSLPEELNR 236
Query: 271 IQIGRSFVAKFCFYPHFNKIVEGCYGRVNIGKDKHTGNSMYRMVKIERVF-LQKPYNMGK 329
+++ R + ++C P F K V GC+ R+ IG H +YR+ +I V K Y +G
Sbjct: 237 VRLSRHKLERWCHMPFFAKTVTGCFVRIGIGN--HNSKPVYRVAEITGVVETAKVYQLGG 294
Query: 330 FFTNQYFGVTQGKDRKIFQMSYFSDSPVTQPEYERYLLSLEKYDITQPSPYILNNKSKAV 389
TN+ + G D+++F++ + S+ T+ E+ ++ ++ + P+ +N K ++
Sbjct: 295 TRTNKGLQLRHGNDQRVFRLEFVSNQEFTESEFMKWKEAMFSAGMQLPTLDEINKKELSI 354
Query: 390 NEFVCEPLTPKLMDDIVRNRMVFNKKLSGTNAVMEKQVLKEKLQFSKQSGDIESESKYAN 449
E + + +++IV+ + F +K A+ + Q+LKEK ++ GD + + +
Sbjct: 355 KEALNYKFNDQDIEEIVKEKERF-RKAPPNYAMKKTQLLKEK-AMAEDLGDQDKAKQIQD 412
Query: 450 QLKALEKRLSSYEKHHENDQNDIKTLGALTSKNRKLNIDRIRHAETIKREEGVNKVDAKS 509
QL LE+R + ++ +I + + +NR+ N I +E E N + +
Sbjct: 413 QLNELEERAEALDRQR---TKNISAISYINQRNREWN---IVESEKALVAESHNMKNQQM 466
Query: 510 DPFSR 514
DPF+R
Sbjct: 467 DPFTR 471
>SPBC651.09c [K] KOG2402 Paf1/RNA polymerase II complex RTF1 component (involved
in regulation of TATA box-binding protein)
Length = 560
Score = 94.0 bits (232), Expect = 6e-19
Identities = 66/252 (26%), Positives = 123/252 (48%), Gaps = 10/252 (3%)
Query: 250 EVEWAEDDNLDRESSLDDYNKIQIGRSFVAKFCFYPHFNKIVEGCYGRVNIGKDKHTGNS 309
+VE DN ++L D N I++GR VA++ ++P F V GC+ RV IG + G
Sbjct: 204 KVEQVSKDNAS--ANLYDLNAIRLGRKHVAEYMYHPIFESTVTGCFVRVKIG--ERDGQG 259
Query: 310 MYRMVKIERVF-LQKPYNMGKFFTNQYFGVTQGKDRKIFQMSYFSDSPVTQPEYERYLLS 368
+YR+ +++ + +KPY + T G+ +++F ++ S+ P + +++R+
Sbjct: 260 VYRLCQVKGILESRKPYRVDGVLTKVSLECFHGRSKRVFDVNVLSNEPFSDHDFQRWHHQ 319
Query: 369 LEKYDITQPSPYILNNKSKAVNEFVCEPLTPKLMDDIVRNRMVFNKKLSGTNAVMEKQVL 428
+ + ++ PS + K + + L+ K + DI+ + ++ S N EK L
Sbjct: 320 MMEDKLSMPSKNFVQRKLNDLRDMSKYVLSEKEVSDIINRKKELSRVPS--NIAAEKTRL 377
Query: 429 KEKLQFSKQSGDIESESKYANQLKALEKRLSSYEKHHENDQNDIKTLGALTSKNRKLNID 488
+++ Q + +G+ E + +QL LE+ LS + N D L + +NR+ N
Sbjct: 378 RQRRQAAYVAGNAELVKEIDDQLNTLEE-LSMGSNQNSNSAMD--QLAKVNERNRRRNHT 434
Query: 489 RIRHAETIKREE 500
IR AE EE
Sbjct: 435 EIRLAEQRMNEE 446
Score = 58.2 bits (139), Expect = 3e-08
Identities = 28/66 (42%), Positives = 43/66 (64%), Gaps = 2/66 (3%)
Query: 89 PFPLEGKYKDEKDREKLELLPEMERESILFERSQQMQKYQERK--LLRQHAKSIKQQQQL 146
P+ LEGK+KDE DR K+ + E+ERESILFER +++ K ER+ +R H ++ + Q
Sbjct: 73 PYRLEGKFKDEADRAKIMAMTEIERESILFEREEEISKLMERRELAIRLHQQNAQYMAQS 132
Query: 147 QREGSK 152
R ++
Sbjct: 133 TRRSTR 138
>At1g61040 [K] KOG2402 Paf1/RNA polymerase II complex RTF1 component (involved
in regulation of TATA box-binding protein)
Length = 643
Score = 84.7 bits (208), Expect = 3e-16
Identities = 91/436 (20%), Positives = 172/436 (38%), Gaps = 39/436 (8%)
Query: 96 YKDEKDREKLELLPEMERESILFERSQQMQKYQERKLLRQHAKSIKQQQQLQREGSKXXX 155
YK+E+DR+KL + E +RE IL ER+ + + LR +S ++ + ++ ++
Sbjct: 104 YKNEEDRQKLAGMTEFQREMILSERADKKGDKNFTEKLRSKRES--EKTPVSKKETQPLP 161
Query: 156 XXXXXXXXXGHSDXXXXXXXXXXXXRAKKSGNYEFSDNXXXXXXXXXXXXXXXXXXXXXX 215
+D RAK+ +
Sbjct: 162 ASRGVRSSARSADRAAAKDDALNELRAKRMKQQD------PAALRKLRDASKGGSGSRDF 215
Query: 216 XXXXXXXXXXXXXXXXXLGYEKSKTRGRERADIHEVEWAEDDNLDRESSLDDYNKIQIGR 275
S+++ + + ++DD D + +D ++ I R
Sbjct: 216 SSTKRKPLASSNLSSSSQSDSDSRSQSDDEGSNGGMLDSDDDRSD-VPTFEDVKEVTIRR 274
Query: 276 SFVAKFCFYPHFNKIVEGCYGRVNIGKDKHTGNSMYRMVKIERVFLQKP---YNMGKFFT 332
S +AK+ P F +++ GC+ RV IG+ K +YR+ ++ V P Y + T
Sbjct: 275 SKLAKWLMEPFFEELIVGCFVRVGIGRSK--SGPIYRLCWVKNVDATDPDKTYKLENKTT 332
Query: 333 NQYFGVTQGKDRKI--FQMSYFSDSPVTQPEYERYLLSLEKYDITQPSPYILNNKSKAV- 389
++Y V G + +QM+ SD + EY +++ +E+ + P+ ++ K +A+
Sbjct: 333 HKYLNVVWGNETSAARWQMAMISDGHPLEEEYRQWIREVERTNGRMPTKQDISEKKEAIQ 392
Query: 390 --NEFVCEPLTPKLMDDIVRNRMVFNKKLSGT---NAVMEKQVLKEKLQFSKQSGDIESE 444
N FV T K +M+ KK + N EK L+++L+ ++ D
Sbjct: 393 RTNSFVYSAETVK--------QMLQEKKSASVRPMNVAAEKDRLRKELEIAQSKNDEAGV 444
Query: 445 SKYANQLKALEKRLSSYEKHHENDQNDIKTLGALTSKNRKLNIDRIRHAETIKREEGVNK 504
+ +++K L+ N + K L N+K + ++A +K K
Sbjct: 445 ERIKSKIKQLDA--------SRNKKGVDKKALKLAEMNKKNRAENFKNASEVKSITASLK 496
Query: 505 V-DAKSDPFSRLKTRT 519
+A DPFSR TR+
Sbjct: 497 AGEAGYDPFSRRWTRS 512
>7291410 [K] KOG2402 Paf1/RNA polymerase II complex RTF1 component (involved
in regulation of TATA box-binding protein)
Length = 775
Score = 80.9 bits (198), Expect = 5e-15
Identities = 61/256 (23%), Positives = 117/256 (44%), Gaps = 10/256 (3%)
Query: 266 DDYNKIQIGRSFVAKFCFYPHFNKIVEGCYGRVNIGKDKHTGNSMYRMVKIERVF-LQKP 324
+D NK+++ R + +F P F V C+ R++IG + +YR+ +I V K
Sbjct: 432 EDLNKLRLSRYKMERFVNLPIFESTVLNCFVRISIGNNGQ--KPVYRVAEIVGVVETGKI 489
Query: 325 YNMGKFFTNQYFGVTQGKDRKIFQMSYFSDSPVTQPEYERYLLSLEKYDITQPSPYILNN 384
Y++G TN+ + G ++F++ + S+ T+ E+ ++ ++ + P+ ++
Sbjct: 490 YSLGTTRTNRGLRLKHGTQERVFRLEFISNQEFTENEFNKWNEVCQQSHVQMPTIDLIAI 549
Query: 385 KSKAVNEFVCEPLTPKLMDDIVRNRMVFNKKLSGTNAVMEKQVLKEKLQFSKQSGDIESE 444
K + + + + +D IV + F + TN M+K L ++ + GD +
Sbjct: 550 KQNDIKKALNYEFKDEDVDKIVEEKNRFRNR--PTNYAMKKTCLMKERDAAMLRGDYDIA 607
Query: 445 SKYANQLKALEKRLSSYEKHHENDQNDIKTLGALTSKNRKLNIDRIRHAETIKREEGVNK 504
Q+ LE R S +K + N + + +NRK N++ A I E NK
Sbjct: 608 QDLGQQIDELENRASELDKRRSHTLN---LISYINDRNRKKNVEDAEKA--ILEEARANK 662
Query: 505 VDAKSDPFSRLKTRTK 520
SDPF+R T+ +
Sbjct: 663 GLKISDPFTRRITQPR 678
>CE09619 [K] KOG2402 Paf1/RNA polymerase II complex RTF1 component (involved
in regulation of TATA box-binding protein)
Length = 613
Score = 70.1 bits (170), Expect = 9e-12
Identities = 77/426 (18%), Positives = 166/426 (38%), Gaps = 18/426 (4%)
Query: 96 YKDEKDREKLELLPEMERESILFERSQQMQKYQERK-LLRQHAKSIKQQQQ--LQREGSK 152
+ D++D+ + + L E+E+E +FER + + R+ + +Q AK K+ + ++ E +
Sbjct: 82 FADKEDKARWKKLTELEKEQEIFERMEARENAIAREEIAQQLAKKAKKSSEKGVKTEKRR 141
Query: 153 XXXXXXXXXXXXGHSDXXXXXXXXXXXXRAKKSGNYEFSDNXXXXXXXXXXXXXXXXXXX 212
N + +
Sbjct: 142 KMNSGGSDAGSPKRKASSDSDSEMDAAFHRPSDINRKHKEKNAMDALKNKRKEIEKKNAK 201
Query: 213 XXXXXXXXXXXXXXXXXXXXLGYEKSKTRGRERADIHEVEWAEDDNLDRE-SSLDDYNKI 271
E S++ R E +D + ++ L + +
Sbjct: 202 NEALSIDAVFGANSGSSSSSSSSESSRSSSSSRESSPERVSEKDKIVKKDVDGLSELRRA 261
Query: 272 QIGRSFVAKFCFYPHFNKIVEGCYGRVNIGKDKHTGNSMYRMVKIERVF-LQKPYNMGKF 330
++ R ++ P F+ V GCY R+ G+ +G S YR+ KI V K Y +
Sbjct: 262 RLSRHKLSLMIHAPFFDSTVVGCYVRLGQGQMSGSG-SKYRIWKIVGVEESNKVYELEGK 320
Query: 331 FTNQYFGVTQGKDRKIFQMSYFSDSPVTQPEYERYLLSLEKYDITQPSPYILNNKSKAVN 390
TN+ G + F+M + S++ Q E++ +LL+ +++ P+ I++ K + +
Sbjct: 321 KTNKIIKCQNGGSERPFRMQFVSNADFEQIEFDEWLLACKRHG-NLPTVDIMDKKKQDIE 379
Query: 391 EFVCEPLTPKLMDDIVRNRMVFNKKLSGTNAVMEKQVLKEKLQFSKQSGDIESESKYANQ 450
+ + + K +D +++ + + + N M K ++ + ++Q GDI + +
Sbjct: 380 KAINHKYSDKEVDLMIKEKSKY--QTVPRNFAMTKANWSKQKELAQQRGDIREAEQIQTK 437
Query: 451 LKALEKRLSSYEKHHENDQNDIKTLGALTSKNRKLNIDRIRHAETIKREEGVNKVDAKSD 510
+ +E++ EK I + + +NR D++ + +K EE +++ D
Sbjct: 438 IDEIERQADELEKER---SKSISAIAFINHRNRSKIKDQVLSGQ-LKIEE-----NSQDD 488
Query: 511 PFSRLK 516
PF+R K
Sbjct: 489 PFTRKK 494
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.314 0.132 0.367
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,422,598
Number of Sequences: 60738
Number of extensions: 979063
Number of successful extensions: 3825
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 3807
Number of HSP's gapped (non-prelim): 13
length of query: 582
length of database: 30,389,216
effective HSP length: 112
effective length of query: 470
effective length of database: 23,586,560
effective search space: 11085683200
effective search space used: 11085683200
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)