ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI0219 suspect: LH UY KOG2274 Intracellular trafficking, secretion, and vesicular transport Predicted importin 9
r_klactI0219 suspect: LH UY KOG2274 Nuclear structure Predicted importin 9
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI0219 76350 79205 952
(952 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGL241w [UY] KOG2274 Predicted importin 9 365 e-100
SPAC22H10.03c [UY] KOG2274 Predicted importin 9 78 7e-14
>YGL241w [UY] KOG2274 Predicted importin 9
Length = 1004
Score = 365 bits (936), Expect = e-100
Identities = 270/1016 (26%), Positives = 478/1016 (46%), Gaps = 84/1016 (8%)
Query: 1 MSIPYLLEGIQSPQKEEREDAENSLLERCATDPPDTFIELIDTATNNNASASTRQLALLC 60
M I L+ G QS K RE AE LL+ C +D F L + A + AS +RQ ALL
Sbjct: 1 MDINELIIGAQSADKHTREVAETQLLQWCDSDASQVFKALANVALQHEASLESRQFALLS 60
Query: 61 LRKFTTMYWSAXXXXXXXXXXXXEQGKDLVRRGLLSLLANEDTEKKVISTVTYCIVQICA 120
LRK TMYWS KD +R LL L N++ K+ + +YCIVQI A
Sbjct: 61 LRKLITMYWSPGFESYRSTSNVEIDVKDFIREVLLKLCLNDNENTKIKNGASYCIVQISA 120
Query: 121 VDFPDEWPGLLDYLNENILNYHSENAISLLTELVQDIITNEMFFDNHSGAKIVNTVLLAL 180
VDFPD+WP LL + + I + HS NA+SLL E+ D+++ EMFF+ G + V L
Sbjct: 121 VDFPDQWPQLLTVIYDAISHQHSLNAMSLLNEIYDDVVSEEMFFEGGIGLATMEIVFKVL 180
Query: 181 NDDTLRLQAKSKLLQLYHHCISQLRNVSMF---VTSELMSEWLIPHLKAMNDCIDKLLES 237
N +T L AK L+L C+ Q+ + + + +S+ L L+ + L +
Sbjct: 181 NTETSTLIAKIAALKLLKACLLQMSSHNEYDEASRKSFVSQCLATSLQILGQL---LTLN 237
Query: 238 YGNNMESEVIGLKGELFMALSKL-FDLNQSILGSNGDLSYRLRITLDAIKSNANSYARAL 296
+GN + K ++ L + D ++ S +++ + + +++ + A
Sbjct: 238 FGNVDVISQLKFKSIIYENLVFIKNDFSRKHFSSELQKQFKI-MAIQDLENVTHINANVE 296
Query: 297 TNNDELRLEIINSSCINTVQYLAYIPSDLIENPTLPDFTEDFIKLCLLPEDYFKL--SDF 354
T E LE ++ I V++L + + + LC L + ++ SDF
Sbjct: 297 TTESEPLLETVHDCSIYIVEFLTSVCTLQFSVEEMNKIITSLTILCQLSSETREIWTSDF 356
Query: 355 NEFISKETGLSASYNARDEIGQYVSSCSDEIYRHITDSVLQKCLQVTSNEAQYQ------ 408
N F+SKETGL+ASYN RD+ ++E + + + L +V SN+ ++
Sbjct: 357 NTFVSKETGLAASYNVRDQ--------ANEFFTSLPNPQLSLIFKVVSNDIEHSTCNYST 408
Query: 409 -EACLFLFQELCSNETSMNVPRYQDFLSLAVMILDD----DACPTFVKSRTILTIPKFFE 463
E+ L+L Q + N+ + L + + L++ P + +R ILTIP+ +
Sbjct: 409 LESLLYLLQCILLNDDEITGENIDQSLQILIKTLENILVSQEIPELILARAILTIPRVLD 468
Query: 464 NNMETLPEIKQLVQQFLVKTVNCTISAEDDFLLASLVISFTYYTSFAELGSILDYQTSMI 523
++ LP+IK L FL K++N + ++ + + ++ +I+FTYY FAEL S+L +
Sbjct: 469 KFIDALPDIKPLTSAFLAKSLNLALKSDKELIKSATLIAFTYYCYFAELDSVLGPEVCSE 528
Query: 524 LQQSLLKAIKTLYVXXXXXXXXXXXXAMHEIVKTWHFQQDLHTKQEIL----NLLLKLSS 579
Q+ +++ I + + +++ + ++ H+++EIL +L+ +SS
Sbjct: 529 TQEKVIRIINQVSSDAEEDTNGALMEVLSQVIS--YNPKEPHSRKEILQAEFHLVFTISS 586
Query: 580 SEPSNVRIVFESVRSLPYLLRDINCTDYTRLCESCFPSFIEAMSTFLQSQQTYSPLVVLA 639
+P+NV++V +S L LL +IN +Y E C PSFI + + + YSPL+ L
Sbjct: 587 EDPANVQVVVQSQECLEKLLDNINMDNYKNYIELCLPSFINVLDSNNANNYRYSPLLSLV 646
Query: 640 LEFLSVFLKNPPVGNHISDEVAEYVLNPIVQFIKQCPDENIAETALQAFVYLACNSNPKF 699
LEF++VFLK P + DE+ +Y+ P+ + + ++ + A +AF YL N++ +
Sbjct: 647 LEFITVFLKKKPNDGFLPDEINQYLFEPLAKVLAFSTEDETLQLATEAFSYLIFNTDTRA 706
Query: 700 WKDSMFDLCSL---VFDPERSLYKTIDVAPIMLLSLRVTQPHENRFIGQIMEYTISKLVD 756
+ + D+ + + E S ++V P+++ IG+I+E + +L+
Sbjct: 707 MEPRLMDIMKVLERLLSLEVSDSAAMNVGPLVVAIFTRFSKEIQPLIGRILEAVVVRLIK 766
Query: 757 PNKLHPVDSFIIISCEVILKDIQSFLAYIFSIPISAEESIPNKLIRELFDCFEENRSKNM 816
+ + + + C + D + + ++ S I +++ ++R+ + FE R +
Sbjct: 767 TQNISTEQNLLSVLCFLTCNDPKQTVDFLSSFQIDNTDAL-TLVMRKWIEAFEVIRGEKR 825
Query: 817 LKEVALSLSELFFSNDQRL------------------TRSSYHDESDGVVR-PFKEYIVK 857
+KE ++LS LFF ND+RL TRS D V+ P I+K
Sbjct: 826 IKENIVALSNLFFLNDKRLQKVVVNGNLIPYEGDLIITRSMAKKMPDRYVQVPLYTKIIK 885
Query: 858 MFADELTRQ-----------TDIXXXXXXXX--------XXXNDITPKDYEEDYV----- 893
+F EL+ Q +DI +D+ D ++Y+
Sbjct: 886 LFVSELSFQSKQPNPEQLITSDIKQEVVNANKDDDNDDWEDVDDVLDYDKLKEYIDDDVD 945
Query: 894 SQLNDDVVLLTGRV-IDMELKEILVQFFKSTQQSNDNSLGRVLKNLDEQERLKILS 948
+ +DD +TG + + + ++LV+FFK + + + + L + ER K+LS
Sbjct: 946 EEADDDSDDITGLMDVKESVVQLLVRFFKEVASKDVSGFHCIYETLSDSER-KVLS 1000
>SPAC22H10.03c [UY] KOG2274 Predicted importin 9
Length = 986
Score = 77.8 bits (190), Expect = 7e-14
Identities = 199/1028 (19%), Positives = 400/1028 (38%), Gaps = 149/1028 (14%)
Query: 6 LLEGIQSPQKEEREDAENSLLERCATDPPDTFIELIDTATNNNASASTRQLALLCLRKFT 65
LLE +QS R AE L R D +L D A++ AS RQ +L+ L+++
Sbjct: 9 LLEQVQSADPNSRIQAELGL--RDLEKYHDFAAKLTDIASSG-ASVPLRQGSLIYLQRYI 65
Query: 66 TMYWSAXXXXXXXXXXXXEQGKDLVRRGLLSLLANEDTEKKVISTVTYCIVQICAVDFPD 125
+WS E K VR LL LL + D +I V Y + I VD+PD
Sbjct: 66 VHHWSPLFEQFQDGPIPDENVKKHVRETLLHLLVSLDNFT-LIKAVAYAVSLIANVDYPD 124
Query: 126 EWP-------GLLDYLNENILNYHSENAISLLTELVQDIITNEMFF--DNHSGAKIVNTV 176
EWP LL NEN +N ++ +L ELV + + E FF + + +
Sbjct: 125 EWPEVVPAVLHLLQSTNENSIN----ASLDVLDELVDESLVEEQFFIIAPQLASILYQFI 180
Query: 177 LLALNDDTLR-LQAKSKLLQLYHHCISQLRNVSMFVTSELMSEWLIPHLKA-MNDCIDKL 234
A +D++R LQA+ ++L+ C+ + E+ E H++ + +
Sbjct: 181 FSAPPNDSMRMLQARG--IKLFRSCLELI---------EIYKETKAEHVRVFLEQILPPW 229
Query: 235 LESYGNNMESEVIG--------------LKGELFMALSKLFDLNQSILGSNGDLSYRLRI 280
++ + + E ++ + GE+ M L+KL +L S L + + +
Sbjct: 230 MDMFSHKFEVSLVDDRQVILPDSCGYFCIMGEIAMTLTKLRELFPSKL--TPYVVTFVEL 287
Query: 281 TLDAIKSNANSYARALTNNDEL---------------RLEIINSSCINTVQYLAYIPSDL 325
+ I+ + Y R + +D L L + S + + S+
Sbjct: 288 VWNIIEKLLDPYIREVVFSDGLDDSAFGDKYPIRYLVELLLFVSVALQSKFVQNLFVSNT 347
Query: 326 IENPTLPDFTEDFIKLCLLPEDYFKL--SDFNEFISKETGLS-ASYNARDEIGQYVSSCS 382
+ P LP ++ LP+ ++ SD +E+I+ E + AS R +S+
Sbjct: 348 VPVPPLPPCIPLLVQYTQLPKHQIEVYESDVSEYIANEFSMDFASDTVRGAAISVLSAFE 407
Query: 383 DEIYRHITDSVLQ-KCLQVTSNEAQ--YQEACLFLFQELCSNETSMNVPRYQDFL----- 434
+ I S+ + + +NE YQEA L+ C + + + Y D+L
Sbjct: 408 EHTTLPIQQSLREMSATYILNNEINWIYQEALLY----ACCSVDAASDDTYDDYLDPIYE 463
Query: 435 SLAVMILDDDACPTFVKSRTILTIPKFFENNMETLPEIKQLVQQFLVKTVNCTISAEDDF 494
++ V I DA P + SR L I F E+ + + QF +N ++A
Sbjct: 464 AIKVRIDYSDA-PILLLSRFFLFIGYFSESTV--------VASQFFQIIMNNLVNA---- 510
Query: 495 LLASLVISFTYYTSFAELGSILDYQTSMILQQSLLKAIKTLYVXXXXXXXXXXXXAMHEI 554
L + + + S+ + + LQ +L+ + A+
Sbjct: 511 -LQVDTVQYAAMKAIERFCSVGKVKPILSLQPMILEVLSQYASKSSDEALVLLVEAISSA 569
Query: 555 VKTWHFQQDLHTKQEILNLLLKL---SSSEPSNVRIVFESVRSLPYLLRDINCTDYTRLC 611
VK + ++ LL L ++S+P I+ ++ + + +Y +C
Sbjct: 570 VKL-DCAKAAELGNSVIPLLFNLVATNASDPYICGIIEDTFEDIIHA-----ANNYESMC 623
Query: 612 ESCFPSFIEAMSTFLQSQQTYSPLVVLALEFLSVFLKNPPVGNHISDEVAEYVLNPIVQF 671
E P ++ ++ Q +V + LS ++ P + + + YVL P+ +
Sbjct: 624 EITLPELLQVLN------QEDPIMVNIGATLLSCLIRAGP--SPLPNGFVGYVLPPVYKI 675
Query: 672 IK-QCPDENIAETALQAFVYLACNSNPKFWKDSMFDLCS---LVFDPERSLYKTIDVAPI 727
+ D + + + + L P+ + + ++F + L K D +
Sbjct: 676 TQIHSGDTELLQLSQEILKGLLEKDTPQLLETEISGSSGFQYILFILHQLLDKESDDSAC 735
Query: 728 MLLS--LRVTQPHENRFIG--QIMEYTISKLVDPNKLHPVDSFIIISCEVILKDIQSFLA 783
L+ L H ++ + I+ I +L + + S I + ++I+KD +
Sbjct: 736 FLVGPILLELADHASQMVDLQSILLSCIKRLAIAEQPRFIQSIIYVFAKLIVKDSLGMMH 795
Query: 784 YIFSIPISAEESIPNKLIRELF-DCFEENRSKNMLKEVALSLSELF-------------- 828
++ S ++ + +++ ++ D F + + + +++++++
Sbjct: 796 FLTSSLLNEQGLTAFEVLMTVWCDNFVYFSNFKNISIICIAMTKIYSFDSPLLDSVQVKG 855
Query: 829 --FSNDQRL---TRSSYHDESDGVVRPFKEYIVKMFADELTRQTDIXXXXXXXXXXXNDI 883
S+ R+ ++S H E V E I+++ ++E + +D
Sbjct: 856 ELISHSNRIITRSQSKLHPEEYSYV-SVGEKILRLLSEEF-----VSLSKDAIVEEVSDD 909
Query: 884 TPKDYEEDYVS------QLNDDV--VLLTGRVIDMELKEILVQFFKSTQQSNDNSLGRVL 935
D+++ +S ND+ V + + +L+ L++FFK +SN +++ V+
Sbjct: 910 GADDWDDGPISAETFGLSANDEFSGVDNSEDEDNTDLQFYLLEFFKEAMKSNLHNINEVV 969
Query: 936 KNLDEQER 943
L ++E+
Sbjct: 970 FRLPQEEQ 977
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.136 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 52,392,550
Number of Sequences: 60738
Number of extensions: 2157501
Number of successful extensions: 6016
Number of sequences better than 1.0e-05: 2
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 6006
Number of HSP's gapped (non-prelim): 6
length of query: 952
length of database: 30,389,216
effective HSP length: 116
effective length of query: 836
effective length of database: 23,343,608
effective search space: 19515256288
effective search space used: 19515256288
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)