ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI0368 good R KOG3109 General function prediction only Haloacid dehalogenase-like hydrolase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI0368 127760 126903 -286
(286 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGL224c [R] KOG3109 Haloacid dehalogenase-like hydrolase 329 3e-90
YER037w [R] KOG3109 Haloacid dehalogenase-like hydrolase 266 3e-71
SPAC24B11.05 [R] KOG3109 Haloacid dehalogenase-like hydrolase 184 1e-46
At3g62040 [R] KOG3109 Haloacid dehalogenase-like hydrolase 89 7e-18
At5g59490 [R] KOG3109 Haloacid dehalogenase-like hydrolase 83 4e-16
At5g59480 [R] KOG3109 Haloacid dehalogenase-like hydrolase 67 3e-11
At5g02230 [R] KOG3109 Haloacid dehalogenase-like hydrolase 64 3e-10
At2g32150 [R] KOG3109 Haloacid dehalogenase-like hydrolase 60 3e-09
ECU05g1170 [R] KOG3109 Haloacid dehalogenase-like hydrolase 54 3e-07
>YGL224c [R] KOG3109 Haloacid dehalogenase-like hydrolase
Length = 280
Score = 329 bits (843), Expect = 3e-90
Identities = 164/275 (59%), Positives = 202/275 (72%), Gaps = 3/275 (1%)
Query: 12 TYSTLSEYTLQTKDKLRINRQHLESLTYPGSKVTFDPDQPIPKP--DPNLKVFFFDIDNC 69
T S L+ Y + +++ N+ HLESLT+PGSKVTF DQ I +PNLKVFFFDIDNC
Sbjct: 6 TASDLATYQNEVNEQIAKNKAHLESLTHPGSKVTFPIDQDISATPQNPNLKVFFFDIDNC 65
Query: 70 LYKKSLRIHDLMEQSIHQYFQMKFNLDNETARELRENYYRTYGLAIKGLVDHHEIDAVEY 129
LYK S RIHDLM+QSI ++FQ L E A L +YY+ YGLAI+GLV H+++A+EY
Sbjct: 66 LYKSSTRIHDLMQQSILRFFQTHLKLSPEDAHVLNNSYYKEYGLAIRGLVMFHKVNALEY 125
Query: 130 NELVDDSLPLQDILKPDPEQRKILQRIRESGCFDKMWLFTNAYKNHGLRCVRLLGIADLF 189
N LVDDSLPLQDILKPD R +L R+R+SG DK+WLFTNAYKNH +RC+RLLGIADLF
Sbjct: 126 NRLVDDSLPLQDILKPDIPLRNMLLRLRQSGKIDKLWLFTNAYKNHAIRCLRLLGIADLF 185
Query: 190 DGITYCDYSQSD-LVCKPDVRAFEKAKLQSGLGEYSNAYFIDDSGSNIRTSIKLGLRKSI 248
DG+TYCDYS++D LVCKP V+AFEKA +SGL Y NAYFIDDSG NI T IKLG++ I
Sbjct: 186 DGLTYCDYSRTDTLVCKPHVKAFEKAMKESGLARYENAYFIDDSGKNIETGIKLGMKTCI 245
Query: 249 HLVEAGFDDFFSTSPSEATIIKAITDLPKACPEIF 283
HLVE ++ +P A +I I +LP ++F
Sbjct: 246 HLVENEVNEILGQTPEGAIVISDILELPHVVSDLF 280
>YER037w [R] KOG3109 Haloacid dehalogenase-like hydrolase
Length = 321
Score = 266 bits (680), Expect = 3e-71
Identities = 125/241 (51%), Positives = 181/241 (74%), Gaps = 7/241 (2%)
Query: 19 YTLQTKDKLRINRQHLESLTYPGSKVTFDPDQP-IPKPDPNLKVFFFDIDNCLYKKSLRI 77
Y Q K ++R+N++HL+SLT+ GS++ F+ D P +P PDP KVFFFDIDN LY+KS ++
Sbjct: 11 YRNQIKKQIRLNQEHLQSLTHLGSQINFEVDPPKLPDPDPARKVFFFDIDNTLYRKSTKV 70
Query: 78 HDLMEQSIHQYFQMKFNLDNETARELRENYYRTYGLAIKGLVDHHEIDAV-EYNELVDDS 136
LM+QS+ +F+ + D++ A L E+YY+ YGL++KGL+ + +ID V +YN +DDS
Sbjct: 71 QLLMQQSLSNFFKYELGFDDDEAERLIESYYQEYGLSVKGLIKNKQIDDVLQYNTFIDDS 130
Query: 137 LPLQDILKPDPEQRKILQRIRES--GCFDKMWLFTNAYKNHGLRCVRLLGIADLFDGITY 194
LPLQD LKPD + R++L +++ G FDK+WLFTN+YKNH +RCV++LGIADLFDGITY
Sbjct: 131 LPLQDYLKPDWKLRELLINLKKKKLGKFDKLWLFTNSYKNHAIRCVKILGIADLFDGITY 190
Query: 195 CDYS---QSDLVCKPDVRAFEKAKLQSGLGEYSNAYFIDDSGSNIRTSIKLGLRKSIHLV 251
C Y + + +CKPD + FE AKLQSGL ++NA+FIDD+ SN+R+++ +G+ IHL+
Sbjct: 191 CHYDRPIEEEFICKPDPKFFETAKLQSGLSSFANAWFIDDNESNVRSALSMGMGHVIHLI 250
Query: 252 E 252
E
Sbjct: 251 E 251
>SPAC24B11.05 [R] KOG3109 Haloacid dehalogenase-like hydrolase
Length = 226
Score = 184 bits (468), Expect = 1e-46
Identities = 90/195 (46%), Positives = 126/195 (64%), Gaps = 3/195 (1%)
Query: 60 KVFFFDIDNCLYKKSLRIHDLMEQSIHQYFQMKFNLDNETARELRENYYRTYGLAIKGLV 119
K+ FFD+DNCLY KS +IH++M I +F K + E A LRE YYR YG+AI+GLV
Sbjct: 6 KIIFFDLDNCLYPKSYKIHNMMAARITAFFSDKLGIPTEEAERLREVYYRHYGIAIRGLV 65
Query: 120 DHHEIDAVEYNELVDDSLPLQDILKPDPEQRKILQRIRESGCFDKMWLFTNAYKNHGLRC 179
HHEIDAV+Y++ VD SLPL+ ++K D R++L +R+ K W+FTNAY H R
Sbjct: 66 LHHEIDAVDYDQRVDQSLPLEKVIKKDEVLREMLLELRKK---YKCWIFTNAYIVHANRV 122
Query: 180 VRLLGIADLFDGITYCDYSQSDLVCKPDVRAFEKAKLQSGLGEYSNAYFIDDSGSNIRTS 239
++ LGI D FDGITYCDY+ DL+ KP +E+ ++G+ + F+DDS NI +
Sbjct: 123 LKYLGIEDCFDGITYCDYNAKDLIAKPMPEMYERVMREAGVTDKDKCIFVDDSYGNILGA 182
Query: 240 IKLGLRKSIHLVEAG 254
+ G + ++ LVE G
Sbjct: 183 REFGWKYTVQLVEHG 197
>At3g62040 [R] KOG3109 Haloacid dehalogenase-like hydrolase
Length = 249
Score = 89.0 bits (219), Expect = 7e-18
Identities = 61/195 (31%), Positives = 97/195 (49%), Gaps = 13/195 (6%)
Query: 58 NLKVFFFDIDNCLYKKSLRIHDLMEQSIHQYFQMKFNLDNETARELRENYYRTYGLAIKG 117
N + FFD+D+ LY S+ I+ +I ++ + ++ +L + Y+ YG + G
Sbjct: 7 NYECLFFDMDDTLYPLSIGINLACRNNIQEFMLNQLGIEESEVPKLCLDLYKEYGTTMAG 66
Query: 118 L-VDHHEIDAVEYNELVDDSLPLQDILKPDPEQRKILQRIRESGCFDKMWLFTNAYKNHG 176
L V +E D E++E V LP + LKPDP R +L + + +FTNA K H
Sbjct: 67 LKVMGYEFDNDEFHEYVHGRLPYEK-LKPDPLLRNLLLSMPH-----RKIIFTNADKAHA 120
Query: 177 LRCVRLLGIADLFDGITYCDY------SQSDLVCKPDVRAFEKAKLQSGLGEYSNAYFID 230
R + LG+ D F+GI + S + ++CKP V AFE A + + + F D
Sbjct: 121 TRALNRLGLEDCFEGIICFETLNPSSDSNTQILCKPSVEAFEAAIRIADIVDPRKTIFFD 180
Query: 231 DSGSNIRTSIKLGLR 245
DS NI ++ GL+
Sbjct: 181 DSIRNIASAKATGLK 195
>At5g59490 [R] KOG3109 Haloacid dehalogenase-like hydrolase
Length = 266
Score = 83.2 bits (204), Expect = 4e-16
Identities = 70/247 (28%), Positives = 114/247 (45%), Gaps = 32/247 (12%)
Query: 57 PNLKVFFFDIDNCLYKKSLRIHDLMEQSIHQYFQMKFNLDNETARELRENYYRTYGLAIK 116
P + FD+D+ LY S + D +I +Y K +D + EL + Y+ YG ++
Sbjct: 9 PRYECLLFDLDDTLYPLSSGLSDACSNNIIEYMVEKLGIDEDGVVELNQILYKKYGTSMA 68
Query: 117 GL-VDHHEIDAVEYNELVDDSLPLQDILKPDPEQRKILQRIRESGCFDKMWLFTNAYKNH 175
GL +E D EY+ V LP ++ LKPDP R +L G + +F+N + H
Sbjct: 69 GLKAVGYEFDNDEYHRYVHGRLPYEN-LKPDPVLRSLLL-----GLPLRKLVFSNGDEVH 122
Query: 176 GLRCVRLLGIADLFDGITYCDYSQSDL-----------------VCKPDVRAFEKAKLQS 218
++ + LGI D F+ I + D+ +CKP AFEKA +
Sbjct: 123 VMKALTRLGIEDCFERIISFETLNPDINEAELSCVTGHLPENPVICKPTEIAFEKAFDIA 182
Query: 219 GLGEYSNAYFIDDSGSNIRTSIKLGLRKSIHLVEAGFDDFFSTSPSEATIIKAITDLPKA 278
L + +F DDS NI+T +GL H V G + S +++I ++ +A
Sbjct: 183 QLNPHKTLFF-DDSTRNIQTGKAVGL----HTVLVGKSEKIDGSD---YALESIHNMKEA 234
Query: 279 CPEIFAK 285
PE++++
Sbjct: 235 FPELWSE 241
>At5g59480 [R] KOG3109 Haloacid dehalogenase-like hydrolase
Length = 282
Score = 67.0 bits (162), Expect = 3e-11
Identities = 56/216 (25%), Positives = 96/216 (43%), Gaps = 42/216 (19%)
Query: 64 FDIDNCLYKKSLRIHDLMEQSIHQYFQMKFNLDNETARELRENYYRTYGLAIKGL-VDHH 122
FDID+ LY S + ++++I +Y K ++ + +EL + Y+ YG + GL +
Sbjct: 20 FDIDDTLYPLSSGLAMEVKKNIQEYMVQKLGIEEDKVQELCLSLYKIYGTTMAGLKAVGY 79
Query: 123 EIDAVEYNELVDDSLPLQDILKPDPEQRKILQRIRESGCFDKMWLFTNAYKNHGLRCVRL 182
+ D +++ V LP LKPDP R I+ + + +FTNA K H + +
Sbjct: 80 DFDYDDFHRFVHGRLPYST-LKPDPILRNIILSLPI-----RKVVFTNADKAHAAKIIAR 133
Query: 183 LGIA---------------------------DLFDGITYC-------DYSQSDLVCKPDV 208
LG+ ++FD I+Y + ++ +VCKP
Sbjct: 134 LGLEGCFEKIISFETLNPITKTESPVDTKTREIFDIISYMANPDSSIELPKTSVVCKPSE 193
Query: 209 RAFEKAKLQSGLGEYSNAYFIDDSGSNIRTSIKLGL 244
AFE+ + + +F DDS NI+T ++GL
Sbjct: 194 GAFEQVFKMANINPKKTLFF-DDSIRNIQTGKRVGL 228
>At5g02230 [R] KOG3109 Haloacid dehalogenase-like hydrolase
Length = 280
Score = 63.5 bits (153), Expect = 3e-10
Identities = 57/213 (26%), Positives = 92/213 (42%), Gaps = 39/213 (18%)
Query: 64 FDIDNCLYKKSLRIHDLMEQSIHQYFQMKFNLDNETARELRENYYRTYGLAIKGL-VDHH 122
FD+D+ LY S I +I Y K + + EL + Y+ YG + GL +
Sbjct: 20 FDLDDTLYPLSSGIARECGNNIKDYMTEKLGIPKDKIVELSDLLYKNYGTTMAGLRAIGY 79
Query: 123 EIDAVEYNELVDDSLPLQDILKPDPEQRKILQRIRESGCFDKMWLFTNAYKNHGLRCVRL 182
E D EY+ V LP +I KPD R +L + + +FTNA + H + ++
Sbjct: 80 EFDYDEYHSFVHGRLPYDNI-KPDLVLRSLLLSLPL-----RKVIFTNADRVHAAKALKK 133
Query: 183 LGIADLFDGITYCD----------------------YSQSD---------LVCKPDVRAF 211
LG+ D F+GI + +++S+ +VCKP A
Sbjct: 134 LGLEDCFEGIICFETLNLMHTNAASNNSEIFDIVGHFNRSEPVGSLPKTPVVCKPSESAI 193
Query: 212 EKAKLQSGLGEYSNAYFIDDSGSNIRTSIKLGL 244
EKA L+ + + F +DS N++ ++GL
Sbjct: 194 EKA-LEIANIDPNRTLFFEDSVRNVQAGKRVGL 225
>At2g32150 [R] KOG3109 Haloacid dehalogenase-like hydrolase
Length = 256
Score = 60.5 bits (145), Expect = 3e-09
Identities = 55/240 (22%), Positives = 102/240 (41%), Gaps = 36/240 (15%)
Query: 59 LKVFFFDIDNCLYKKSLRIHDLMEQSIHQYFQMKFNLDNETARELRENYYRTYGLAIKGL 118
+ FD+D+ LY I ++++I + KF A LR ++TYG + GL
Sbjct: 6 INCLIFDLDDTLYPLKTGIAPAVKKNIDDFLVEKFGFSESKASSLRVELFKTYGSTLAGL 65
Query: 119 -VDHHEIDAVEYNELVDDSLPLQDILKPDPEQRKILQRIRESGCFDKMWLFTNAYKNHGL 177
H++ EY+ V LP I +P+ + R +L +I++ NH +
Sbjct: 66 RALGHDVHPDEYHSFVHGRLPYGSI-EPNNKLRNLLNKIKQRKI------------NHAV 112
Query: 178 RCVRLLGIADLFDGITYCDYSQSDL------------VCKPDVRAFEKAKLQSGLGEYSN 225
+ ++ LG+ D F+ + + +L V KP + A + ++ +
Sbjct: 113 KVLKKLGLEDCFEEMICFETMNPNLFGSTTRPDEYPVVLKPSLTAMDIC-IRVANVDPRR 171
Query: 226 AYFIDDSGSNIRTSIKLGLRK-SIHLVEAGFDDFFSTSPSEATIIKAITDLPKACPEIFA 284
F+DD+ NI +GLR + E D ++ ++ +T++ A PEI+A
Sbjct: 172 TVFLDDNIHNITAGKSVGLRTILVGRAEKTKDADYA--------VETVTEIATAVPEIWA 223
>ECU05g1170 [R] KOG3109 Haloacid dehalogenase-like hydrolase
Length = 324
Score = 53.5 bits (127), Expect = 3e-07
Identities = 45/191 (23%), Positives = 80/191 (41%), Gaps = 8/191 (4%)
Query: 53 PKPDPNLKVFFFDIDNCLYKKSLRIHDLMEQSIHQYFQMKFNLDNETARELRENYYRTYG 112
P+ N +F +DID+ LY S + ++ + + + F +L + E+ E
Sbjct: 96 PRKIGNDILFLYDIDDTLYHPSNNLQEMERKFLVEKF---LSLKEGSTPEMFEEQLNVAL 152
Query: 113 LAIKGLVDHHEIDAVEYNELVDDSLPLQDILKPDPEQRKILQRIRESGCFDKMWLFTNAY 172
L + + EY E++ + LQ L PD + R L ++ + FTN
Sbjct: 153 LYSALFYKYGNLSLEEYWEMISEFDYLQ-YLSPDMDLRNFLLSMKNV----RKCCFTNGP 207
Query: 173 KNHGLRCVRLLGIADLFDGITYCDYSQSDLVCKPDVRAFEKAKLQSGLGEYSNAYFIDDS 232
++ + +GI D F+ + CKP ++E G+ N YF DDS
Sbjct: 208 RDRAENILTKIGILDCFEVVVCIGKYDKTFCCKPLSESYEFVTKVLGIESPGNVYFFDDS 267
Query: 233 GSNIRTSIKLG 243
+NI + ++G
Sbjct: 268 ENNIIKAREIG 278
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.321 0.140 0.413
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,090,584
Number of Sequences: 60738
Number of extensions: 811156
Number of successful extensions: 1940
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1920
Number of HSP's gapped (non-prelim): 11
length of query: 286
length of database: 30,389,216
effective HSP length: 105
effective length of query: 181
effective length of database: 24,011,726
effective search space: 4346122406
effective search space used: 4346122406
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)