ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI0403 good R KOG4131 General function prediction only Ngg1-interacting factor 3 protein NIF3L1
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI0403 135920 135066 -285
(285 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGL221c [R] KOG4131 Ngg1-interacting factor 3 protein NIF3L1 330 2e-90
SPCC126.12 [R] KOG4131 Ngg1-interacting factor 3 protein NIF3L1 208 8e-54
7298295 [R] KOG4131 Ngg1-interacting factor 3 protein NIF3L1 155 5e-38
Hs14731460 [R] KOG4131 Ngg1-interacting factor 3 protein NIF3L1 102 6e-22
Hs11141899 [R] KOG4131 Ngg1-interacting factor 3 protein NIF3L1 102 6e-22
ECU07g0100 [R] KOG4131 Ngg1-interacting factor 3 protein NIF3L1 82 9e-16
>YGL221c [R] KOG4131 Ngg1-interacting factor 3 protein NIF3L1
Length = 288
Score = 330 bits (845), Expect = 2e-90
Identities = 170/290 (58%), Positives = 212/290 (72%), Gaps = 9/290 (3%)
Query: 1 MSRALSRAQLKSVIKKISQLYPESYADSAWDNTGLLIDCS---VDTEQSHAKTQPKVLLT 57
MSRA++RAQL +++ I++ YP+ YAD +WDNTGLLIDCS V T ++AKT KVLLT
Sbjct: 1 MSRAITRAQLDKLVRSITKFYPQKYADKSWDNTGLLIDCSTAQVTTADANAKT--KVLLT 58
Query: 58 IDLTESVAKEALAKECNLIVAYHPFIFPSWKKLTPHSNPQHASAIKLIQRGISVYSPHTA 117
+DLT+SVA+EA+ CN+IVAYHPFIFPSW +L+PH+NPQH +AIKLIQ GISVY PHTA
Sbjct: 59 VDLTKSVAQEAVDANCNVIVAYHPFIFPSWNRLSPHTNPQHETAIKLIQYGISVYCPHTA 118
Query: 118 IDAAKNGVNDWXXXXXXXXXXXXXXIERVTPVQDEEEHEVGYGRVVDLNEPLSLKDIVNN 177
+DAA+ GVNDW T V E + +GYGR V+ N+ +SL+ IV N
Sbjct: 119 VDAARGGVNDWLVRGLNNGENVAKSYALET-VSGETDDLIGYGRFVEFNKDISLEQIVKN 177
Query: 178 VKDTLDLSFVQVAT---KNELSDHKIKKVALCAGSGSGVFKSLREDVDLYYTGELSHHEV 234
VK L + +VQVA+ + + KIKKVA+CAGSGSGVFK L+EDVDLYYTGE+SHHEV
Sbjct: 178 VKRVLRVPYVQVASLAAPSAWNQLKIKKVAVCAGSGSGVFKQLKEDVDLYYTGEMSHHEV 237
Query: 235 LRYKESGKAVIVCNHSNTERGFLRKVMHPSLTKSGIDCIVSETDIDPLQV 284
L++KE GK VIVCNHSNTERGFL+ VM L G + +VS+ D DPL V
Sbjct: 238 LKWKEMGKTVIVCNHSNTERGFLQDVMKGLLQDEGHEVVVSKMDCDPLTV 287
>SPCC126.12 [R] KOG4131 Ngg1-interacting factor 3 protein NIF3L1
Length = 278
Score = 208 bits (529), Expect = 8e-54
Identities = 130/288 (45%), Positives = 168/288 (58%), Gaps = 23/288 (7%)
Query: 5 LSRAQLKSVIKKISQ-LYPESYADSAWDNTGLLIDCSVDTEQSHAKTQPKVLLTIDLTES 63
+S+A + S +KK+ + +Y ADS WDNTGLL++ + + VLLTIDLTE
Sbjct: 1 MSKANISSKLKKVVESIYNPKLADS-WDNTGLLLEAPFPRTNASS-----VLLTIDLTEK 54
Query: 64 VAKEALA-KECNLIVAYHPFIFPSWKKLTPHSNPQHASAIKLIQRGISVYSPHTAIDAAK 122
VA+EA++ K + IVAYHP IF K +T +PQ S +KL GI VYSPHTA+DAA
Sbjct: 55 VAEEAISNKLVSSIVAYHPIIFRGLKAITME-DPQQRSLLKLAAEGIHVYSPHTAVDAAV 113
Query: 123 NGVNDWXXXXXXXXXXXXXXIERVTPVQDEE--EHEVGYGRVVDLNEPLSLKDIVNNVKD 180
+GVNDW I+ V P Q GYGR+ +L P +L+++V K+
Sbjct: 114 DGVNDWLAQGIAGGRNN---IKSVVPTQQNSVMAEAEGYGRICELKIPTTLRELVQRAKE 170
Query: 181 TLDLSFVQVATKNELSDHKIKKVALCAGSGSGVFKSLREDVDLYYTGELSHHEVLRYKES 240
L +VQV N L H I KV+LCAGSG V + D DLY+TGELSHH+VL
Sbjct: 171 LTGLQYVQVCAPNGLDSH-ISKVSLCAGSGGSVV--MNTDADLYFTGELSHHQVLAAMAK 227
Query: 241 GKAVIVCNHSNTERGFLRKVMHPSLT----KSGIDC--IVSETDIDPL 282
G +VI+C HSNTERG+L+ VM L K G+D IVS D DPL
Sbjct: 228 GISVILCGHSNTERGYLKDVMCQKLASSFHKEGVDANVIVSSMDADPL 275
>7298295 [R] KOG4131 Ngg1-interacting factor 3 protein NIF3L1
Length = 288
Score = 155 bits (393), Expect = 5e-38
Identities = 103/283 (36%), Positives = 146/283 (51%), Gaps = 22/283 (7%)
Query: 6 SRAQLKSVIKKISQLYPESYADSAWDNTGLLIDCSVDTEQSHAKTQPKVLLTIDLTESVA 65
S +L +V+K++ P S+A+ WDN GLLI E K K+LLT DLTE V
Sbjct: 5 SGQKLAAVVKELENFAPTSWAEK-WDNVGLLI------EPHREKQIKKILLTNDLTEPVV 57
Query: 66 KEALAKECNLIVAYHPFIFPSWKKLTPHSNPQHASAIKLIQRGISVYSPHTAIDAAKNGV 125
KEAL KE LI++YHP IF ++T S+ + + I++YSPHTA D GV
Sbjct: 58 KEALEKEAELIISYHPPIFKPLTRIT-QSHWKERVVAACLANDIALYSPHTAWDKKSGGV 116
Query: 126 NDWXXXXXXXXXXXXXXIERVTPVQDEEEHEVGYGRVVDLNEPLSLKDIVNNVKDTLDLS 185
NDW I + P++ E G G + + L +V +++ + S
Sbjct: 117 NDWLSKAVN--------IISIRPLEPELGAPPGTGSGRYIETKMELSQVVESLQKRIRNS 168
Query: 186 FVQVATKNELSDHKIKKVALCAGSGSGVFKSLREDVDLYYTGELSHHEVLRYKESGKAVI 245
I+ V +CAGSG+ + K ++ DL TGE+SHHEVL + + V+
Sbjct: 169 VHVALAVGHTPKTLIQSVGICAGSGASLLKGIQ--ADLIITGEMSHHEVLEFTHNNTTVL 226
Query: 246 VCNHSNTERGFLRKVMHPSLTKS-GIDCI--VSETDIDPLQVI 285
+CNHSN+ERGFL + P L KS +C+ VSE D DPL +
Sbjct: 227 LCNHSNSERGFLHEFC-PILAKSLNEECLVFVSEVDKDPLVTV 268
>Hs14731460 [R] KOG4131 Ngg1-interacting factor 3 protein NIF3L1
Length = 377
Score = 102 bits (254), Expect = 6e-22
Identities = 59/131 (45%), Positives = 84/131 (64%), Gaps = 5/131 (3%)
Query: 158 GYGRVVDLNEPLSLKDIVNNVKDTLDLSFVQVATK-NELSDHKIKKVALCAGSGSGVFKS 216
G GR+ L+E +SL +++ +K L LS +++A + ++K VALCAGSGS V +
Sbjct: 249 GMGRLCTLDESVSLATMIDRIKRHLKLSHIRLALGVGRTLESQVKVVALCAGSGSSVLQG 308
Query: 217 LREDVDLYYTGELSHHEVLRYKESGKAVIVCNHSNTERGFLRKV--MHPSLTKSGIDCIV 274
+ + DLY TGE+SHH+ L G VI+C HSNTERGFL + M S ++ I+ I+
Sbjct: 309 V--EADLYLTGEMSHHDTLDAASQGINVILCEHSNTERGFLSDLRDMLDSHLENKINIIL 366
Query: 275 SETDIDPLQVI 285
SETD DPLQV+
Sbjct: 367 SETDRDPLQVV 377
Score = 85.1 bits (209), Expect = 1e-16
Identities = 58/187 (31%), Positives = 93/187 (49%), Gaps = 14/187 (7%)
Query: 10 LKSVIKKISQLYPESYADSAWDNTGLLIDCSVDTEQSHAKTQPKVLLTIDLTESVAKEAL 69
LK+++ ++ S+A+S WDN GLL+ E S T + LT DLTE V +E L
Sbjct: 30 LKALLSSLNDFASLSFAES-WDNVGLLV------EPSPPHTVNTLFLTNDLTEEVMEEVL 82
Query: 70 AKECNLIVAYHPFIFPSWKKLTPHSNPQHASAIKLIQRGISVYSPHTAIDAAKNGVNDWX 129
K+ +LI++YHP IF K++T ++ + I+ ++ + +YSPHTA DAA GVN+W
Sbjct: 83 QKKADLILSYHPPIFRPMKRITWNTWKERL-VIRALENRVGIYSPHTAYDAAPQGVNNWL 141
Query: 130 XXXXXXXXXXXXXIERVTPVQDEEEHEVGYGRVVDLNEPLSLKDIVNNVK--DTLDLSFV 187
+ E H V + ++N L +++ VK D + ++
Sbjct: 142 AKGLGACTSRPIHPSKAPNYPTEGNHRVEF----NVNYTQDLDKVMSAVKGIDGVSVTSF 197
Query: 188 QVATKNE 194
T NE
Sbjct: 198 SARTGNE 204
>Hs11141899 [R] KOG4131 Ngg1-interacting factor 3 protein NIF3L1
Length = 351
Score = 102 bits (254), Expect = 6e-22
Identities = 59/131 (45%), Positives = 84/131 (64%), Gaps = 5/131 (3%)
Query: 158 GYGRVVDLNEPLSLKDIVNNVKDTLDLSFVQVATK-NELSDHKIKKVALCAGSGSGVFKS 216
G GR+ L+E +SL +++ +K L LS +++A + ++K VALCAGSGS V +
Sbjct: 223 GMGRLCTLDESVSLATMIDRIKRHLKLSHIRLALGVGRTLESQVKVVALCAGSGSSVLQG 282
Query: 217 LREDVDLYYTGELSHHEVLRYKESGKAVIVCNHSNTERGFLRKV--MHPSLTKSGIDCIV 274
+ + DLY TGE+SHH+ L G VI+C HSNTERGFL + M S ++ I+ I+
Sbjct: 283 V--EADLYLTGEMSHHDTLDAASQGINVILCEHSNTERGFLSDLRDMLDSHLENKINIIL 340
Query: 275 SETDIDPLQVI 285
SETD DPLQV+
Sbjct: 341 SETDRDPLQVV 351
Score = 86.3 bits (212), Expect = 5e-17
Identities = 57/187 (30%), Positives = 90/187 (47%), Gaps = 13/187 (6%)
Query: 10 LKSVIKKISQLYPESYADSAWDNTGLLIDCSVDTEQSHAKTQPKVLLTIDLTESVAKEAL 69
LK+++ ++ S+A+S WDN GLL+ E S T + LT DLTE V +E L
Sbjct: 3 LKALLSSLNDFASLSFAES-WDNVGLLV------EPSPPHTVNTLFLTNDLTEEVMEEVL 55
Query: 70 AKECNLIVAYHPFIFPSWKKLTPHSNPQHASAIKLIQRGISVYSPHTAIDAAKNGVNDWX 129
K+ +LI++YHP IF K++ + I+ ++ + +YSPHTA DAA GVN+W
Sbjct: 56 QKKADLILSYHPPIFRPMKRINLEHMGRERLVIRALENRVGIYSPHTAYDAAPQGVNNWL 115
Query: 130 XXXXXXXXXXXXXIERVTPVQDEEEHEVGYGRVVDLNEPLSLKDIVNNVK--DTLDLSFV 187
+ E H V + ++N L +++ VK D + ++
Sbjct: 116 AKGLGACTSRPIHPSKAPNYPTEGNHRVEF----NVNYTQDLDKVMSAVKGIDGVSVTSF 171
Query: 188 QVATKNE 194
T NE
Sbjct: 172 SARTGNE 178
>ECU07g0100 [R] KOG4131 Ngg1-interacting factor 3 protein NIF3L1
Length = 242
Score = 82.0 bits (201), Expect = 9e-16
Identities = 72/280 (25%), Positives = 124/280 (43%), Gaps = 44/280 (15%)
Query: 10 LKSVIKKISQLYPESYADSAWDNTGLLIDCSVDTEQSHAKTQPKVLLTIDLTESVAKEAL 69
+ V K I + A + WDN G+++D + K+LLTIDLTE V +E +
Sbjct: 3 IHEVSKAIDRFASLRRAKTDWDNVGVIVDSGTQDK--------KILLTIDLTEPVLEECI 54
Query: 70 AKECNLIVAYHPFIFPSWKKLTPHSNPQHASAIKLIQRGISVYSPHTAIDAAKNGVNDWX 129
+VAYHP IF + KKL + + I I+ GI+V++PH+A+D N
Sbjct: 55 HLGVKNVVAYHPIIFKAVKKL----GSKESIVIGCIKNGINVFTPHSALDPLMN------ 104
Query: 130 XXXXXXXXXXXXXIERVTPVQDEEEHEVGYGRVVDLNEPLSLKDIVNNVKDTLDLSFVQV 189
T V + + + ++ + + +K+ L ++
Sbjct: 105 -----------------TYVYNMINSGPFFYKKNCGPNTSTIGNAIRILKERSGLESFRI 147
Query: 190 ATKNELSDHKIKKVALCAGSGSG-VFKSLREDVDLYYTGELSHHEVLRYKESGKAVIVCN 248
E H ++ V G G F+++ + TGE+SHH++L +G +VI+
Sbjct: 148 CLARE---HTMESVPEFMHVGVGATFRNVLLKNSIVITGEMSHHDLLSCIANGASVILME 204
Query: 249 HSNTER---GFLRKVMHPSLTKSGIDCIVSETDIDPLQVI 285
HSN+ER G + + L + + VS+ D DP+ ++
Sbjct: 205 HSNSERICLGHISAKLKEELPE--YEIFVSKNDKDPVTIL 242
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.132 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,621,603
Number of Sequences: 60738
Number of extensions: 602618
Number of successful extensions: 1602
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1572
Number of HSP's gapped (non-prelim): 9
length of query: 285
length of database: 30,389,216
effective HSP length: 105
effective length of query: 180
effective length of database: 24,011,726
effective search space: 4322110680
effective search space used: 4322110680
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)