ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI0434 good R KOG4155 General function prediction only FOG: WD40 repeat
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI0434 147235 146033 -401
(401 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGL213c [R] KOG4155 FOG: WD40 repeat 388 e-108
SPBC32F12.02 [R] KOG4155 FOG: WD40 repeat 82 1e-15
7299285 [R] KOG4155 FOG: WD40 repeat 59 2e-08
Hs19913371 [B] KOG0273 Beta-transducin family (WD-40 repeat) pro... 53 7e-07
Hs5032159 [B] KOG0273 Beta-transducin family (WD-40 repeat) protein 52 2e-06
CE03924 [Z] KOG0318 WD40 repeat stress protein/actin interacting... 50 8e-06
>YGL213c [R] KOG4155 FOG: WD40 repeat
Length = 397
Score = 388 bits (997), Expect = e-108
Identities = 195/406 (48%), Positives = 267/406 (65%), Gaps = 16/406 (3%)
Query: 1 MSKLFIPTTNCGKAHETDIYGLVITNPYTVSCSGDGYLKLWNNKLTEDQLAKDHVIKEFV 60
MSK+FI T N GKAH+ DI+ + N +TVSCSGDGYLK+W+NKL +++ KD FV
Sbjct: 1 MSKVFIATANAGKAHDADIFSVSACNSFTVSCSGDGYLKVWDNKLLDNENPKDKSYSHFV 60
Query: 61 TPMGLHHVSAFESVESNGETVIILSAISFDGKIYFYQI----DLKEGKLVKLSLLNADDS 116
GLHHV +++E + + +++ SF G + FY+I + K+ KL LL++D
Sbjct: 61 HKSGLHHVDVLQAIERDAFELCLVATTSFSGDLLFYRITREDETKKVIFEKLDLLDSDMK 120
Query: 117 KHSYWACQWYISTNPKNPHKFIATTVQGTTKVWNFIIFQEPELDTETGEPLSNSLVYEPH 176
KHS+WA +W S + H+ +AT V+GTT +W F F + E S +L + P
Sbjct: 121 KHSFWALKWGASNDRLLSHRLVATDVKGTTYIWKFHPFAD--------ESNSLTLNWSPT 172
Query: 177 FNFQGKINS-QTP-RFATAVDVSSTQLIATGFSDGSVVVSQLSTLRPVYNFEGFGLKGVE 234
QG + S TP +FAT+VD+S LIATGF++G+V +S+LSTLRP+YNFE +
Sbjct: 173 LELQGTVESPMTPSQFATSVDISERGLIATGFNNGTVQISELSTLRPLYNFES--QHSMI 230
Query: 235 ESSSTIRDIKFSPAGSLLAVAHDSGSYGCVSLYETEFGERIGNFTVPTHGNQTTIASYAH 294
+S++IR +KFSP GSLLA+AHDS S+GC++LYETEFGERIG+ +VPTH +Q ++ +AH
Sbjct: 231 NNSNSIRSVKFSPQGSLLAIAHDSNSFGCITLYETEFGERIGSLSVPTHSSQASLGEFAH 290
Query: 295 NGWVFSVSFNSTGEYLASAGYDGKIRIWDTKSRERVSTLNVNTTXXXXXXXXXXXXXHGD 354
+ WV S+SFN +GE L SAG+DGK+R WD K++ER++TLN++ HGD
Sbjct: 291 SSWVMSLSFNDSGETLCSAGWDGKLRFWDVKTKERITTLNMHCDDIEIEEDILAVDEHGD 350
Query: 355 SLEVPPVLAVSFFDKCVRSGMGGSTNEGICCVCSDRSIRWYREAGG 400
SL P V V F K RSGMG NE +CCVC DRSIRW+REAGG
Sbjct: 351 SLAEPGVFDVKFLKKGWRSGMGADLNESLCCVCLDRSIRWFREAGG 396
>SPBC32F12.02 [R] KOG4155 FOG: WD40 repeat
Length = 302
Score = 82.0 bits (201), Expect = 1e-15
Identities = 92/389 (23%), Positives = 149/389 (37%), Gaps = 107/389 (27%)
Query: 14 AHETDIYGLVITNPYTVSCSGDGYLKLWNNKLTEDQLAKDHVIKEFVTP--MGLHHVSAF 71
AH+ DIY L + S SGD +K W+ E L ++E TP +G+HH++
Sbjct: 14 AHQADIYSLNVVAGNLWSASGDSKIKKWSIGDAEHSL-----VEEIDTPHKLGVHHLAT- 67
Query: 72 ESVESNGETVIILSAISFDGKIYFYQIDLKEGKLVKLSLLNADDSKHSYWACQWYISTNP 131
S E V++ + F +Y + + E + D ++ + + S
Sbjct: 68 ----SLDENVVV--SCGFGQDVYVWNPETNEFR---------DLGNNAQHPSECWSSCIS 112
Query: 132 KNPHKFIATTVQGTTKVW-NFIIFQEPELDTETGEPLSNSLVYEPHFNFQGKINSQTPRF 190
+ T+V G VW N + ELDT+ +F
Sbjct: 113 PDGQTIAFTSVDGRIAVWDNPSDCKISELDTKG-------------------------KF 147
Query: 191 ATAVDVSST-QLIATGFSDGSVVVSQLSTLRPVYNFEGFGLKGVEESSSTIRDIKFSPAG 249
+D S + I +G G + + T R + G +S +R + FSP
Sbjct: 148 GLCIDYSPNGRFIVSGHQTGQLFLISTETGRLFHVLSGH--------TSPVRSVAFSPGS 199
Query: 250 SLLAVAHDSGSYGCVSLYETEFGERIGNFTVPTHGNQTTIASYAHNGWVFSVSFNSTGEY 309
+LLA A DS +++Y+ G+++G H W+F+V+FN G+
Sbjct: 200 TLLAAAGDSKM---ITIYDVLSGDQVGQLR-------------GHAAWIFAVAFNPVGDL 243
Query: 310 LASAGYDGKIRIWDTKSRERVSTLNVNTTXXXXXXXXXXXXXHGDSLEVPPVLAVSFFDK 369
L SA +GKI+IWD + E +ST S + AV+++
Sbjct: 244 LLSADVEGKIKIWDIDTMECIST---------------------QSETDGAIWAVAWY-- 280
Query: 370 CVRSGMGGSTNEGICCVCSDRSIRWYREA 398
G +D+SIRWYR A
Sbjct: 281 ----------KNGFIVAGADKSIRWYRAA 299
>7299285 [R] KOG4155 FOG: WD40 repeat
Length = 331
Score = 58.5 bits (140), Expect = 2e-08
Identities = 38/156 (24%), Positives = 70/156 (44%), Gaps = 25/156 (16%)
Query: 183 INSQTPRFATAVDVSST-QLIATGFSDGSVVVSQLSTLRPVYNFEGFGLKGVEESSSTIR 241
+++Q ++ ++ S + IA+G DG + + ++ + V EG + +R
Sbjct: 165 LDAQNGKYTLSIAYSPDGKYIASGAIDGIITIFDVAAGKVVQTLEGHAMP--------VR 216
Query: 242 DIKFSPAGSLLAVAHDSGSYGCVSLYETEFGERIGNFTVPTHGNQTTIASYAHNGWVFSV 301
+ FSP LL A D G + LY+ + +G + H WV V
Sbjct: 217 SLCFSPNSQLLLTASDDGH---MKLYDVTHSDVVGTLS-------------GHASWVLCV 260
Query: 302 SFNSTGEYLASAGYDGKIRIWDTKSRERVSTLNVNT 337
+F+ G++ AS+ D ++IWDT R+ + T +T
Sbjct: 261 AFSEDGKHFASSSSDNSVKIWDTSERKCLHTFAEHT 296
>Hs19913371 [B] KOG0273 Beta-transducin family (WD-40 repeat) protein
Length = 514
Score = 53.1 bits (126), Expect = 7e-07
Identities = 83/342 (24%), Positives = 134/342 (38%), Gaps = 90/342 (26%)
Query: 13 KAHETDIYGLVITNPYT---VSCSGDGYLKLWNNKLTED------QLAKDHVIKEFVTPM 63
+ HE++++ + NP + S SGD ++WN L+E+ QL H I+E
Sbjct: 166 RGHESEVF-ICAWNPVSDLLASGSGDSTARIWN--LSENSTSGSTQLVLRHCIRE----- 217
Query: 64 GLHHVSAFESVES---NGETVIILSAISFDGKIYFYQIDLKEGKLVKLSLLNADDSKHSY 120
G V + + V S N E ++ + S+DG F +I K+G L K
Sbjct: 218 GGQDVPSNKDVTSLDWNSEGTLLATG-SYDG---FARIWTKDGNLAS----TLGQHKGPI 269
Query: 121 WACQWYISTNPKNPHKFIATTVQGTTKVWNFIIFQEPELDTETGEPLSNSLVYEPHFNFQ 180
+A +W K + ++ V TT +W D TGE + F F
Sbjct: 270 FALKWN-----KKGNFILSAGVDKTTIIW----------DAHTGEA-------KQQFPFH 307
Query: 181 GKINSQTPRFATAVDVSSTQLIATGFSDGSVVVSQLSTLRPVYNFEGFGLKGVEESSSTI 240
A VD S A+ +D + V +L RP+ F+G ++ +
Sbjct: 308 SAP-------ALDVDWQSNNTFASCSTDMCIHVCKLGQDRPIKTFQGH--------TNEV 352
Query: 241 RDIKFSPAGSLLAVAHDSGSYGCVSLYETEFGERIGNFTVPTHGNQTTIASYAHNGWVFS 300
IK+ P G+LLA D + S+ + H Q AHN +++
Sbjct: 353 NAIKWDPTGNLLASCSDDMTLKIWSMKQDNC----------VHDLQ------AHNKEIYT 396
Query: 301 VSFNSTGE---------YLASAGYDGKIRIWDTKSRERVSTL 333
+ ++ TG LASA +D +R+WD + TL
Sbjct: 397 IKWSPTGPGTNNPNANLMLASASFDSTVRLWDVDRGICIHTL 438
>Hs5032159 [B] KOG0273 Beta-transducin family (WD-40 repeat) protein
Length = 577
Score = 51.6 bits (122), Expect = 2e-06
Identities = 82/332 (24%), Positives = 131/332 (38%), Gaps = 90/332 (27%)
Query: 13 KAHETDIYGLVITNPYT---VSCSGDGYLKLWNNKLTED----QLAKDHVIKEFVTPMGL 65
+ HE++++ + NP + S SGD ++WN + QL H I+E G
Sbjct: 229 RGHESEVF-ICAWNPVSDLLASGSGDSTARIWNLNENSNGGSTQLVLRHCIRE-----GG 282
Query: 66 HHVSAFESVES-----NGETVIILSAISFDGKIYFYQIDLKEGKLVKLSLLNADDSKHSY 120
H V + + V S NG +L+ S+DG F +I ++G L K
Sbjct: 283 HDVPSNKDVTSLDWNTNGT---LLATGSYDG---FARIWTEDGNLAS----TLGQHKGPI 332
Query: 121 WACQWYISTNPKNPHKFIATTVQGTTKVWNFIIFQEPELDTETGEPLSNSLVYEPHFNFQ 180
+A +W N K + ++ V TT +W D TGE + F F
Sbjct: 333 FALKW----NRKGNY-ILSAGVDKTTIIW----------DAHTGEA-------KQQFPFH 370
Query: 181 GKINSQTPRFATAVDVSSTQLIATGFSDGSVVVSQLSTLRPVYNFEGFGLKGVEESSSTI 240
A VD + A+ +D + V +L RPV F+G ++ +
Sbjct: 371 SAP-------ALDVDWQNNTTFASCSTDMCIHVCRLGCDRPVKTFQGH--------TNEV 415
Query: 241 RDIKFSPAGSLLAVAHDSGSYGCVSLYETEFGERIGNFTVPTHGNQTTIASYAHNGWVFS 300
IK+ P+G LLA D + S+ + V H Q AHN +++
Sbjct: 416 NAIKWDPSGMLLASCSDDMTLKIWSMKQE----------VCIHDLQ------AHNKEIYT 459
Query: 301 VSFNSTGE---------YLASAGYDGKIRIWD 323
+ ++ TG LASA +D +R+WD
Sbjct: 460 IKWSPTGPATSNPNSNIMLASASFDSTVRLWD 491
>CE03924 [Z] KOG0318 WD40 repeat stress protein/actin interacting protein
Length = 611
Score = 49.7 bits (117), Expect = 8e-06
Identities = 37/137 (27%), Positives = 66/137 (48%), Gaps = 19/137 (13%)
Query: 202 IATGFSDGSVVVSQLSTLRPVYNFEG-FGLKGVEESSSTIRDIKFSPAGSLLAVAHDSGS 260
I +G D +V + + P + F+ FG E + + ++++P GSL A +G
Sbjct: 163 IISGSDDNTVAIFE----GPPFKFKSTFG-----EHTKFVHSVRYNPDGSLFA---STGG 210
Query: 261 YGCVSLYETEFGERIGNFTVPTHGNQTTIASYAHNGWVFSVSFNSTGEYLASAGYDGKIR 320
G + LY G + G F ++ + AH+G VF ++++ G +ASA D I+
Sbjct: 211 DGTIVLYNGVDGTKTGVF------EDDSLKNVAHSGSVFGLTWSPDGTKIASASADKTIK 264
Query: 321 IWDTKSRERVSTLNVNT 337
IW+ + + T+ V T
Sbjct: 265 IWNVATLKVEKTIPVGT 281
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.134 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,182,348
Number of Sequences: 60738
Number of extensions: 1008622
Number of successful extensions: 4820
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 4797
Number of HSP's gapped (non-prelim): 21
length of query: 401
length of database: 30,389,216
effective HSP length: 109
effective length of query: 292
effective length of database: 23,768,774
effective search space: 6940482008
effective search space used: 6940482008
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)