ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI0453 good IR KOG1269 General function prediction only SAM-dependent methyltransferases
r_klactI0453 good IR KOG1269 Lipid transport and metabolism SAM-dependent methyltransferases
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI0453 153498 152386 -371
(371 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YML008c [IR] KOG1269 SAM-dependent methyltransferases 586 e-167
SPBC16E9.05 [IR] KOG1269 SAM-dependent methyltransferases 392 e-109
At5g13710 [IR] KOG1269 SAM-dependent methyltransferases 301 1e-81
At1g76090 [IR] KOG1269 SAM-dependent methyltransferases 219 7e-57
At1g20330 [IR] KOG1269 SAM-dependent methyltransferases 217 3e-56
CE20965 [IR] KOG1269 SAM-dependent methyltransferases 106 6e-23
At1g64970 [IR] KOG1269 SAM-dependent methyltransferases 74 3e-13
CE11068 [IR] KOG1269 SAM-dependent methyltransferases 63 8e-10
At1g73600 [IR] KOG1269 SAM-dependent methyltransferases 57 6e-08
At3g18000 [IR] KOG1269 SAM-dependent methyltransferases 51 2e-06
>YML008c [IR] KOG1269 SAM-dependent methyltransferases
Length = 383
Score = 586 bits (1510), Expect = e-167
Identities = 283/370 (76%), Positives = 316/370 (84%), Gaps = 2/370 (0%)
Query: 1 MSESNELRKRQAEFTKELHGNNV-TKSGFSALVSKNKSAQTEAVAKYLKHWDGKTDADAE 59
MSE+ ELRKRQA+FT+ELHG+++ K+G SAL+SKN SAQ EAV KYL++WDG+TD DAE
Sbjct: 1 MSET-ELRKRQAQFTRELHGDDIGKKTGLSALMSKNNSAQKEAVQKYLRNWDGRTDKDAE 59
Query: 60 RRRLEDYNESTHSYYNVVTDFYEYGWGSSFHFSRFFKGESFSASVARHEHYLAYKAGIKE 119
RRLEDYNE+THSYYNVVTDFYEYGWGSSFHFSRF+KGESF+AS+ARHEHYLAYKAGI+
Sbjct: 60 ERRLEDYNEATHSYYNVVTDFYEYGWGSSFHFSRFYKGESFAASIARHEHYLAYKAGIQR 119
Query: 120 NDLILDVGCGVGGPARTISRFTGCNIIGLNNNDYQIQKANYYAKRDHLDSKLSFVKGDFM 179
DL+LDVGCGVGGPAR I+RFTGCN+IGLNNNDYQI KA YYAK+ +L ++ FVKGDFM
Sbjct: 120 GDLVLDVGCGVGGPAREIARFTGCNVIGLNNNDYQIAKAKYYAKKYNLSDQMDFVKGDFM 179
Query: 180 KMEFDENTFDSVYAIEATCHAPTFEGVYGEIYKVLKPGGTFAVYEWVMTDKYDETNPEHR 239
KM+F+ENTFD VYAIEATCHAP EGVY EIYKVLKPGGTFAVYEWVMTDKYDE NPEHR
Sbjct: 180 KMDFEENTFDKVYAIEATCHAPKLEGVYSEIYKVLKPGGTFAVYEWVMTDKYDENNPEHR 239
Query: 240 KIAYEIELGDGIPKMYSVDAARDALSKVGFXXXXXXXXXXXXXXXPWYAPLTGEWKYVNS 299
KIAYEIELGDGIPKM+ VD AR AL GF PWY PLTGEWKYV +
Sbjct: 240 KIAYEIELGDGIPKMFHVDVARKALKNCGFEVLVSEDLADNDDEIPWYYPLTGEWKYVQN 299
Query: 300 VADLATFFRTSRLGRAFTTSMVTVFEKLGLAPKGSVSVTNALEEAAVGLVAGGEKKLFTP 359
+A+LATFFRTS LGR FTT+MVTV EKLGLAP+GS VT ALE AAVGLVAGG+ KLFTP
Sbjct: 300 LANLATFFRTSYLGRQFTTAMVTVMEKLGLAPEGSKEVTAALENAAVGLVAGGKSKLFTP 359
Query: 360 MMLFVAKKPE 369
MMLFVA+KPE
Sbjct: 360 MMLFVARKPE 369
>SPBC16E9.05 [IR] KOG1269 SAM-dependent methyltransferases
Length = 378
Score = 392 bits (1008), Expect = e-109
Identities = 192/360 (53%), Positives = 246/360 (68%), Gaps = 5/360 (1%)
Query: 15 TKELHGNNVTK-SGFSALVSKNKSAQTEAVAKYLKHWDGKTDADAER---RRLEDYNEST 70
++ LHG K +G +A+ SK+ Q+ + +Y + WD + ++E RR++ Y
Sbjct: 16 SRRLHGKAAEKKTGLAAIASKDVDEQSRKLQEYFEFWDRNHENESEEDRARRIDGYKSVV 75
Query: 71 HSYYNVVTDFYEYGWGSSFHFSRFFKGESFSASVARHEHYLAYKAGIKENDLILDVGCGV 130
+SYY++ TD YEYGW SFHFSRF+KGE+F+ S+ARHEHYLAY+ GIK +LDVGCGV
Sbjct: 76 NSYYDLATDLYEYGWSQSFHFSRFYKGEAFAQSIARHEHYLAYRMGIKPGSRVLDVGCGV 135
Query: 131 GGPARTISRFTGCNIIGLNNNDYQIQKANYYAKRDHLDSKLSFVKGDFMKMEFDENTFDS 190
GGPAR I+ FTGCN++GLNNNDYQI + N YA + +LD K FVKGDFM M F++NTFD
Sbjct: 136 GGPAREITEFTGCNLVGLNNNDYQISRCNNYAVKRNLDKKQVFVKGDFMHMPFEDNTFDY 195
Query: 191 VYAIEATCHAPTFEGVYGEIYKVLKPGGTFAVYEWVMTDKYDETNPEHRKIAYEIELGDG 250
VYAIEAT HAP+ EGVYGEI++VLKPGG F VYEWVM+D YD + P+HR+IAY IE+GDG
Sbjct: 196 VYAIEATVHAPSLEGVYGEIFRVLKPGGVFGVYEWVMSDDYDSSIPKHREIAYNIEVGDG 255
Query: 251 IPKMYSVDAARDALSKVGFXXXXXXXXXXXXX-XXPWYAPLTGEWKYVNSVADLATFFRT 309
IP+M A +A+ KVGF PWY PLTG+ ++ D+ T FRT
Sbjct: 256 IPQMVRKCDAVEAIKKVGFNLLEEDDLTDHDNPDLPWYYPLTGDITKCQNIWDVFTVFRT 315
Query: 310 SRLGRAFTTSMVTVFEKLGLAPKGSVSVTNALEEAAVGLVAGGEKKLFTPMMLFVAKKPE 369
SRLG+ T V EK+G+A KG+ V + L A GL+ GGE LFTPM L +AKKPE
Sbjct: 316 SRLGKLVTRYSVQFLEKIGVAAKGTSKVGDTLAIAQKGLIEGGETHLFTPMFLMIAKKPE 375
>At5g13710 [IR] KOG1269 SAM-dependent methyltransferases
Length = 336
Score = 301 bits (771), Expect = 1e-81
Identities = 156/335 (46%), Positives = 208/335 (61%), Gaps = 10/335 (2%)
Query: 35 NKSAQTEAVAKYLKHWDGKTDADAERRRLEDYNESTHSYYNVVTDFYEYGWGSSFHFSRF 94
+KS AV KY ++ + ER+ +Y + + YY++ T FYEYGWG SFHF++
Sbjct: 12 DKSDVLTAVEKYEQYHVFHGGNEEERKA--NYTDMVNKYYDLATSFYEYGWGESFHFAQR 69
Query: 95 FKGESFSASVARHEHYLAYKAGIKENDLILDVGCGVGGPARTISRFTGCNIIGLNNNDYQ 154
+KGES S+ RHEH+LA + GI+ +LDVGCG+GGP R I+RF+ + GLNNN+YQ
Sbjct: 70 WKGESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPLREIARFSNSVVTGLNNNEYQ 129
Query: 155 IQKANYYAKRDHLDSKLSFVKGDFMKMEFDENTFDSVYAIEATCHAPTFEGVYGEIYKVL 214
I + + +D +FVK DFMKM F EN+FD+VYAIEATCHAP G Y EIY+VL
Sbjct: 130 ITRGKELNRLAGVDKTCNFVKADFMKMPFPENSFDAVYAIEATCHAPDAYGCYKEIYRVL 189
Query: 215 KPGGTFAVYEWVMTDKYDETNPEHRKIAYEIELGDGIPKMYSVDAARDALSKVGFXXXXX 274
KPG FA YEW MTD +D N EH+KI EIE+GDG+P + +AL + GF
Sbjct: 190 KPGQCFAAYEWCMTDAFDPDNAEHQKIKGEIEIGDGLPDIRLTTKCLEALKQAGF-EVIW 248
Query: 275 XXXXXXXXXXPWYAPLTGEWKYVNSVADLATFFRTSRLGRAFTTSMVTVFEKLGLAPKGS 334
PWY PL ++S FR + +GR T +MV + E + LAP+GS
Sbjct: 249 EKDLAKDSPVPWYLPLDKNHFSLSS-------FRLTAVGRFITKNMVKILEYIRLAPQGS 301
Query: 335 VSVTNALEEAAVGLVAGGEKKLFTPMMLFVAKKPE 369
V+N LE+AA GLV GG +++FTPM F+A+KPE
Sbjct: 302 QRVSNFLEQAAEGLVDGGRREIFTPMYFFLARKPE 336
>At1g76090 [IR] KOG1269 SAM-dependent methyltransferases
Length = 359
Score = 219 bits (557), Expect = 7e-57
Identities = 116/299 (38%), Positives = 161/299 (53%), Gaps = 18/299 (6%)
Query: 72 SYYNVVTDFYEYGWGSSFHFSRFFKGESFSASVARHEHYLAYKAGIKENDLILDVGCGVG 131
++YN+VTD YE+GWG SFHFS G+S + HE +K ILD GCGVG
Sbjct: 76 TFYNLVTDIYEWGWGQSFHFSPHVPGKSDKDATRIHEEMAVDLIKVKPGQKILDAGCGVG 135
Query: 132 GPARTISRFTGCNIIGLNNNDYQIQKANYYAKRDHLDSKLSFVKGDFMKMEFDENTFDSV 191
GP R I+ + + G+ N+YQ+Q+A + K+ LDS + V G+F+KM FDENTFD
Sbjct: 136 GPMRAIAAHSKAQVTGITINEYQVQRAKLHNKKAGLDSLCNVVCGNFLKMPFDENTFDGA 195
Query: 192 YAIEATCHAPTFEGVYGEIYKVLKPGGTFAVYEWVMTDKYDETNPEHRKIAYEIELGDGI 251
Y+IEATCHAP E VY EI++V+KPG F YEWV T+KY + + EH+ + IE GD +
Sbjct: 196 YSIEATCHAPKLEEVYSEIFRVMKPGSLFVSYEWVTTEKYRDDDEEHKDVIQGIERGDAL 255
Query: 252 PKMYSVDAARDALSKVGFXXXXXXXXXXXXXXXPWYAPLTGEWKYVNSVADLATFFRTSR 311
P + S KVGF PW+ L + R
Sbjct: 256 PGLRSYADIAVTAKKVGF-EVVKEKDLAKPPSKPWWNRL-----------------KMGR 297
Query: 312 LGRAFTTSMVTVFEKLGLAPKGSVSVTNALEEAAVGLVAGGEKKLFTPMMLFVAKKPEQ 370
+ +V + +G+APKG+V V L + A L GGE +F+PM + + +KPE+
Sbjct: 298 IAYWRNHVVVVILSAIGVAPKGTVDVHKMLFKTADYLTRGGETGIFSPMHMILCRKPEK 356
>At1g20330 [IR] KOG1269 SAM-dependent methyltransferases
Length = 361
Score = 217 bits (552), Expect = 3e-56
Identities = 121/345 (35%), Positives = 173/345 (50%), Gaps = 18/345 (5%)
Query: 25 KSGFSALVSKNKSAQTEAVAKYLKHWDGKTDADAERRRLEDYNESTHSYYNVVTDFYEYG 84
+ G A+ S E V K + E E + ++YN+VTD YE+G
Sbjct: 29 RKGKRAVDLSGGSISAEKVQDNYKQYWSFFRRPKEIETAEKVPDFVDTFYNLVTDIYEWG 88
Query: 85 WGSSFHFSRFFKGESFSASVARHEHYLAYKAGIKENDLILDVGCGVGGPARTISRFTGCN 144
WG SFHFS G+S + HE +K ILDVGCGVGGP R I+ + N
Sbjct: 89 WGQSFHFSPSIPGKSHKDATRLHEEMAVDLIQVKPGQKILDVGCGVGGPMRAIASHSRAN 148
Query: 145 IIGLNNNDYQIQKANYYAKRDHLDSKLSFVKGDFMKMEFDENTFDSVYAIEATCHAPTFE 204
++G+ N+YQ+ +A + K+ LD+ V G+F++M FD+N+FD Y+IEATCHAP E
Sbjct: 149 VVGITINEYQVNRARLHNKKAGLDALCEVVCGNFLQMPFDDNSFDGAYSIEATCHAPKLE 208
Query: 205 GVYGEIYKVLKPGGTFAVYEWVMTDKYDETNPEHRKIAYEIELGDGIPKMYSVDAARDAL 264
VY EIY+VLKPG + YEWV T+K+ + EH ++ IE GD +P + + +
Sbjct: 209 EVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDDEHVEVIQGIERGDALPGLRAYVDIAETA 268
Query: 265 SKVGFXXXXXXXXXXXXXXXPWYAPLTGEWKYVNSVADLATFFRTSRLGRAFTTSMVTVF 324
KVGF PW+ T + RL +V +
Sbjct: 269 KKVGF-EIVKEKDLASPPAEPWW-----------------TRLKMGRLAYWRNHIVVQIL 310
Query: 325 EKLGLAPKGSVSVTNALEEAAVGLVAGGEKKLFTPMMLFVAKKPE 369
+G+APKG+V V L + A L GGE +F+PM + + +KPE
Sbjct: 311 SAVGVAPKGTVDVHEMLFKTADYLTRGGETGIFSPMHMILCRKPE 355
>CE20965 [IR] KOG1269 SAM-dependent methyltransferases
Length = 366
Score = 106 bits (264), Expect = 6e-23
Identities = 60/178 (33%), Positives = 94/178 (52%), Gaps = 2/178 (1%)
Query: 65 DYNESTHSYYNVVTDFYEYGWGSSFHF-SRFFKGESFSASVARHEHYLAYKAGIKENDLI 123
D+ E T YY+V++ + +G +FHF F+G+ ++ ++A K + EN
Sbjct: 40 DHLEVTSHYYSVMSTVIDEYFGGNFHFVPPKFEGQKLEEALKSLHCHIAEKLELSENVHC 99
Query: 124 LDVGCGVGGPARTISRFTGCNIIGLNNNDYQIQKANYYAKRDHLDSKLSFVKGDFMKMEF 183
LD+GCG+GG I+ F G + G+ + + N + + V D KM F
Sbjct: 100 LDIGCGIGGVMLDIADF-GAKLTGVTIAPNEAEIGNEKFANMGISDRCKIVAADCQKMPF 158
Query: 184 DENTFDSVYAIEATCHAPTFEGVYGEIYKVLKPGGTFAVYEWVMTDKYDETNPEHRKI 241
+++TFD YAI + + P + V EI +VLKPGG F VY+ + T+ YD+ N EH KI
Sbjct: 159 EDSTFDVAYAIYSLKYIPNLDKVMKEIQRVLKPGGKFIVYDLIKTNDYDKDNKEHYKI 216
>At1g64970 [IR] KOG1269 SAM-dependent methyltransferases
Length = 348
Score = 73.9 bits (180), Expect = 3e-13
Identities = 49/188 (26%), Positives = 79/188 (41%), Gaps = 15/188 (7%)
Query: 52 GKTDADAERRRLEDYNESTHSYYNVVTDFYEYGWGSSFHFSRFFKGESFSASVARH---- 107
G A E + +YN + +E WG H + S S + H
Sbjct: 46 GNVAVAAAATSTEALRKGIAEFYNETSGLWEEIWGDHMHHGFYDPDSSVQLSDSGHKEAQ 105
Query: 108 ----EHYLAYKAGIKEND------LILDVGCGVGGPARTISRFTGCNIIGLNNNDYQIQK 157
E L + AG+ + + ++DVGCG+GG +R ++ G IG+ + Q ++
Sbjct: 106 IRMIEESLRF-AGVTDEEEEKKIKKVVDVGCGIGGSSRYLASKFGAECIGITLSPVQAKR 164
Query: 158 ANYYAKRDHLDSKLSFVKGDFMKMEFDENTFDSVYAIEATCHAPTFEGVYGEIYKVLKPG 217
AN A L K SF D + F++ FD V+++E+ H P E+ +V PG
Sbjct: 165 ANDLAAAQSLAHKASFQVADALDQPFEDGKFDLVWSMESGEHMPDKAKFVKELVRVAAPG 224
Query: 218 GTFAVYEW 225
G + W
Sbjct: 225 GRIIIVTW 232
>CE11068 [IR] KOG1269 SAM-dependent methyltransferases
Length = 437
Score = 62.8 bits (151), Expect = 8e-10
Identities = 41/154 (26%), Positives = 77/154 (49%), Gaps = 11/154 (7%)
Query: 65 DYNESTHSYYNVVTDFYEYGWGSSFHFSRFFKGESFSASVARHEHYLAYKAGIKENDLIL 124
D+ + T Y N D YE+ +G +F + ++ + + +K G +L
Sbjct: 180 DFLDKTQ-YTNTGIDAYEWMFGVNF-----ISPGGYDENLKIIKRFGDFKPG----QTML 229
Query: 125 DVGCGVGGPARTISRFTGCNIIGLNNNDYQIQKANYYAKRDHLDSKLSFVKGDFMKMEFD 184
D+G G+GG AR ++ G ++ G++ + + A + DS++ + D + +F+
Sbjct: 230 DIGVGIGGGARQVADEFGVHVHGIDLSSNMLAIA-LERLHEEKDSRVKYSITDALVYQFE 288
Query: 185 ENTFDSVYAIEATCHAPTFEGVYGEIYKVLKPGG 218
+N+FD V++ + H P E ++ IYK LKPGG
Sbjct: 289 DNSFDYVFSRDCIQHIPDTEKLFSRIYKALKPGG 322
>At1g73600 [IR] KOG1269 SAM-dependent methyltransferases
Length = 555
Score = 56.6 bits (135), Expect = 6e-08
Identities = 32/114 (28%), Positives = 56/114 (49%), Gaps = 2/114 (1%)
Query: 117 IKENDLILDVGCGVGGPARTISRFTGCNIIGLNNNDYQIQKANYYAKRDHLDSKLSFVKG 176
+K +LDVGCG+GG ++ +++G++ + I A +A L + F
Sbjct: 344 LKPGQKVLDVGCGIGGGDFYMAENFDVDVVGIDLSVNMISFALEHAIG--LKCSVEFEVA 401
Query: 177 DFMKMEFDENTFDSVYAIEATCHAPTFEGVYGEIYKVLKPGGTFAVYEWVMTDK 230
D K E+ +NTFD +Y+ + H ++ YK LKPGG + ++ + K
Sbjct: 402 DCTKKEYPDNTFDVIYSRDTILHIQDKPALFRRFYKWLKPGGKVLITDYCRSPK 455
>At3g18000 [IR] KOG1269 SAM-dependent methyltransferases
Length = 289
Score = 51.2 bits (121), Expect = 2e-06
Identities = 28/118 (23%), Positives = 55/118 (45%), Gaps = 4/118 (3%)
Query: 114 KAGIKENDLILDVGCGVGGPARTISRFTGCNIIGLNNNDYQIQKANYYAKRD-HLDSKLS 172
K +K +LDVGCG+GG ++ +++G+ D + ++ +R L +
Sbjct: 75 KMNLKPGQKVLDVGCGIGGGDFYMAEKFDVHVVGI---DLSVNMISFALERAIGLSCSVE 131
Query: 173 FVKGDFMKMEFDENTFDSVYAIEATCHAPTFEGVYGEIYKVLKPGGTFAVYEWVMTDK 230
F D + +N+FD +Y+ + H ++ +K LKPGG + ++ + K
Sbjct: 132 FEVADCTTKHYPDNSFDVIYSRDTILHIQDKPALFRTFFKWLKPGGKVLISDYCRSPK 189
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.133 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,501,530
Number of Sequences: 60738
Number of extensions: 907385
Number of successful extensions: 2158
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 2139
Number of HSP's gapped (non-prelim): 10
length of query: 371
length of database: 30,389,216
effective HSP length: 108
effective length of query: 263
effective length of database: 23,829,512
effective search space: 6267161656
effective search space used: 6267161656
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)