ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI0539 good C KOG1647 Energy production and conversion Vacuolar H+-ATPase V1 sector, subunit D
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI0539 181274 182101 276
(276 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YEL051w [C] KOG1647 Vacuolar H+-ATPase V1 sector subunit D 412 e-115
SPCC965.03 [C] KOG1647 Vacuolar H+-ATPase V1 sector subunit D 281 5e-76
Hs7706757 [C] KOG1647 Vacuolar H+-ATPase V1 sector subunit D 235 6e-62
CE00209 [C] KOG1647 Vacuolar H+-ATPase V1 sector subunit D 230 1e-60
7303058 [C] KOG1647 Vacuolar H+-ATPase V1 sector subunit D 226 4e-59
At3g58730 [C] KOG1647 Vacuolar H+-ATPase V1 sector subunit D 218 6e-57
ECU10g0140 [C] KOG1647 Vacuolar H+-ATPase V1 sector subunit D 143 2e-34
7303490 [C] KOG1647 Vacuolar H+-ATPase V1 sector subunit D 130 3e-30
>YEL051w [C] KOG1647 Vacuolar H+-ATPase V1 sector subunit D
Length = 256
Score = 412 bits (1058), Expect = e-115
Identities = 222/276 (80%), Positives = 238/276 (85%), Gaps = 20/276 (7%)
Query: 1 MSSNREQVFPTRMTLGLMKTKLKGANQGYSLLKRKSEALTKRFRDITKRIDDSKQKMGRV 60
MS NREQVFPTRMTLGLMKTKLKGANQGYSLLKRKSEALTKRFRDITKRIDD+KQKMGRV
Sbjct: 1 MSGNREQVFPTRMTLGLMKTKLKGANQGYSLLKRKSEALTKRFRDITKRIDDAKQKMGRV 60
Query: 61 MQTAAFSLAEVTYATGENIGYQVQENVANARFKVRATQENVSGVYLPQFESFIDSNINDF 120
MQTAAFSLAEV+YATGENIGYQVQE+V+ ARFKVRA QENVSGVYL QFES+ID INDF
Sbjct: 61 MQTAAFSLAEVSYATGENIGYQVQESVSTARFKVRARQENVSGVYLSQFESYIDPEINDF 120
Query: 121 KMTGLGRGGQQVQRAKEIYSRAVETLVELASLQTAFIILDEVIKVTNRRVNAIEHVIIPR 180
++TGLGRGGQQVQRAKEIYSRAVETLVELASLQTAFIILDEVIKVTNRRVNAIEHVIIPR
Sbjct: 121 RLTGLGRGGQQVQRAKEIYSRAVETLVELASLQTAFIILDEVIKVTNRRVNAIEHVIIPR 180
Query: 181 TENTIAYINSELDELDREEFYRLKKVQEKKQIQTAQLDAEMKQKKLDEANAESEGKPDSQ 240
TENTIAYINSELDELDREEFYRLKKVQEKKQ +TA+LDAEMK K+ + D+
Sbjct: 181 TENTIAYINSELDELDREEFYRLKKVQEKKQNETAKLDAEMKLKR-------DRAEQDAS 233
Query: 241 EATKTGVEAADTQEQEEQSGPQVEALITEQEEDVIF 276
E AAD + PQ E L+ +QE+DVIF
Sbjct: 234 EV------AADEE-------PQGETLVADQEDDVIF 256
>SPCC965.03 [C] KOG1647 Vacuolar H+-ATPase V1 sector subunit D
Length = 285
Score = 281 bits (720), Expect = 5e-76
Identities = 144/275 (52%), Positives = 200/275 (72%), Gaps = 4/275 (1%)
Query: 2 SSNREQVFPTRMTLGLMKTKLKGANQGYSLLKRKSEALTKRFRDITKRIDDSKQKMGRVM 61
S RE VFPTRMTL MKT+LKGA G+SLLKRKSEAL KRFR+I I+ +KQKMGRVM
Sbjct: 3 SKQRENVFPTRMTLTTMKTRLKGAQTGHSLLKRKSEALKKRFREIVVNIEQAKQKMGRVM 62
Query: 62 QTAAFSLAEVTYATGENIGYQVQENVANARFKVRATQENVSGVYLPQFESFIDSNINDFK 121
Q AAFS+AEV +A G NI +++Q++V R +VR+ QEN+SGV+LP FE +D +I+DF+
Sbjct: 63 QIAAFSMAEVGFAMGNNINFEIQQSVKQPRLRVRSKQENISGVFLPTFEMNLDESIDDFQ 122
Query: 122 MTGLGRGGQQVQRAKEIYSRAVETLVELASLQTAFIILDEVIKVTNRRVNAIEHVIIPRT 181
+TGLG+GGQQ+Q+A+++Y +AVETLV+LAS Q+AF++L +V+++TNRRVN+IEH+IIPR
Sbjct: 123 LTGLGKGGQQIQKARQVYEKAVETLVQLASYQSAFVLLGDVLQMTNRRVNSIEHIIIPRL 182
Query: 182 ENTIAYINSELDELDREEFYRLKKVQEKKQIQTAQLDAEMKQKKLDEANAESEGKPDS-- 239
ENTI YI SEL+EL+RE+F RLKKVQ+ K+ + D+ K++ +N + K
Sbjct: 183 ENTIKYIESELEELEREDFTRLKKVQKTKE-NAEKADSVTKEEHQGGSNTLQQTKDVGGA 241
Query: 240 -QEATKTGVEAADTQEQEEQSGPQVEALITEQEED 273
A + G E + E + + + T+ EE+
Sbjct: 242 IAPAAEVGKEVINEVENSKDDTYSLPSTSTDDEEE 276
>Hs7706757 [C] KOG1647 Vacuolar H+-ATPase V1 sector subunit D
Length = 247
Score = 235 bits (599), Expect = 6e-62
Identities = 125/240 (52%), Positives = 175/240 (72%), Gaps = 7/240 (2%)
Query: 4 NREQVFPTRMTLGLMKTKLKGANQGYSLLKRKSEALTKRFRDITKRIDDSKQKMGRVMQT 63
+R ++FP+RM +MK +LKGA G +LLK+KS+ALT RFR I K+I ++K MG VM+
Sbjct: 5 DRIEIFPSRMAQTIMKARLKGAQTGRNLLKKKSDALTLRFRQILKKIIETKMLMGEVMRE 64
Query: 64 AAFSLAEVTYATGENIGYQVQENVANARFKVRATQENVSGVYLPQFESFIDSNINDFKMT 123
AAFSLAE + G+ V +NV A+ K+RA ++NV+GV LP FE + + + +++T
Sbjct: 65 AAFSLAEAKFTAGD-FSTTVIQNVNKAQVKIRAKKDNVAGVTLPVFEHYHEGT-DSYELT 122
Query: 124 GLGRGGQQVQRAKEIYSRAVETLVELASLQTAFIILDEVIKVTNRRVNAIEHVIIPRTEN 183
GL RGG+Q+ + K Y++AVE LVELASLQT+F+ LDE IK+TNRRVNAIEHVIIPR E
Sbjct: 123 GLARGGEQLAKLKRNYAKAVELLVELASLQTSFVTLDEAIKITNRRVNAIEHVIIPRIER 182
Query: 184 TIAYINSELDELDREEFYRLKKVQEKKQIQTAQLDAEMKQKK-----LDEANAESEGKPD 238
T+AYI +ELDE +REEFYRLKK+QEKK+I + + +++Q++ L+ AN +E K +
Sbjct: 183 TLAYIITELDEREREEFYRLKKIQEKKKILKEKSEKDLEQRRAAGEVLEPANLLAEEKDE 242
>CE00209 [C] KOG1647 Vacuolar H+-ATPase V1 sector subunit D
Length = 257
Score = 230 bits (587), Expect = 1e-60
Identities = 126/239 (52%), Positives = 171/239 (70%), Gaps = 10/239 (4%)
Query: 4 NREQVFPTRMTLGLMKTKLKGANQGYSLLKRKSEALTKRFRDITKRIDDSKQKMGRVMQT 63
+R VFP+RM LMKT+LKGA +G+SLLK+K++AL RFRDI ++I ++K MG VM+
Sbjct: 7 DRIAVFPSRMAQTLMKTRLKGAQKGHSLLKKKADALNLRFRDILRKIVENKVLMGEVMKE 66
Query: 64 AAFSLAEVTYATGENIGYQVQENVANARFKVRATQENVSGVYLPQFESFIDSNINDFKMT 123
AAFSLAE + G+ + V +NV+ A+++VR +ENV GV+LP F+++ D + + +T
Sbjct: 67 AAFSLAEAKFTAGD-FSHTVIQNVSQAQYRVRMKKENVVGVFLPVFDAYQDGP-DAYDLT 124
Query: 124 GLGRGGQQVQRAKEIYSRAVETLVELASLQTAFIILDEVIKVTNRRVNAIEHVIIPRTEN 183
GLG+GG + R K+ Y++A+E LVELA+LQT FI LDE IKVTNRRVNAIEHVIIPR EN
Sbjct: 125 GLGKGGANIARLKKNYNKAIELLVELATLQTCFITLDEAIKVTNRRVNAIEHVIIPRIEN 184
Query: 184 TIAYINSELDELDREEFYRLKKVQEKKQIQTAQLDAEMKQKKLD-----EANAESEGKP 237
T+ YI +ELDE++REEF+R+KK+Q K+ Q E QK L+ E A SE P
Sbjct: 185 TLTYIVTELDEMEREEFFRMKKIQANKKKLKEQ---EAAQKALEGPGPGEDAAHSENNP 240
>7303058 [C] KOG1647 Vacuolar H+-ATPase V1 sector subunit D
Length = 246
Score = 226 bits (575), Expect = 4e-59
Identities = 126/241 (52%), Positives = 172/241 (71%), Gaps = 8/241 (3%)
Query: 4 NREQVFPTRMTLGLMKTKLKGANQGYSLLKRKSEALTKRFRDITKRIDDSKQKMGRVMQT 63
+R +FP+R LMK +L GA +G+ LLK+K++AL RFR I +I ++K MG VM+
Sbjct: 5 DRLPIFPSRGAQMLMKARLAGAQKGHGLLKKKADALQMRFRLILGKIIETKTLMGDVMKE 64
Query: 64 AAFSLAEVTYATGENIGYQVQENVANARFKVRATQENVSGVYLPQFESFIDSNINDFKMT 123
AAFSLAE + +G+ I V +NV A+ K+R ++NV+GV LP FES+ D + + +++
Sbjct: 65 AAFSLAEAKFTSGD-INQVVLQNVTKAQIKIRTKKDNVAGVTLPVFESYQDGS-DTYELA 122
Query: 124 GLGRGGQQVQRAKEIYSRAVETLVELASLQTAFIILDEVIKVTNRRVNAIEHVIIPRTEN 183
GL RGGQQ+ + K+ Y AV+ LVELASLQT+F+ LDEVIK+TNRRVNAIEHVIIPR +
Sbjct: 123 GLARGGQQLAKLKKNYQSAVKLLVELASLQTSFVTLDEVIKITNRRVNAIEHVIIPRIDR 182
Query: 184 TIAYINSELDELDREEFYRLKKVQEKKQIQTAQLD---AEMKQKKLD---EANAESEGKP 237
T+AYI SELDEL+REEFYRLKK+Q+KK+ + D AE+ Q+ +D +AN EG
Sbjct: 183 TLAYIISELDELEREEFYRLKKIQDKKREARIKADAKKAELLQQGIDVRQQANILDEGDD 242
Query: 238 D 238
D
Sbjct: 243 D 243
>At3g58730 [C] KOG1647 Vacuolar H+-ATPase V1 sector subunit D
Length = 261
Score = 218 bits (556), Expect = 6e-57
Identities = 111/219 (50%), Positives = 158/219 (71%), Gaps = 1/219 (0%)
Query: 5 REQVFPTRMTLGLMKTKLKGANQGYSLLKRKSEALTKRFRDITKRIDDSKQKMGRVMQTA 64
R V PT LG+MK +L GA +G++LLK+KS+ALT +FR + K+I +K+ MG +M+T+
Sbjct: 7 RLNVVPTVTMLGVMKARLVGATRGHALLKKKSDALTVQFRALLKKIVTAKESMGDMMKTS 66
Query: 65 AFSLAEVTYATGENIGYQVQENVANARFKVRATQENVSGVYLPQFESFIDSNINDFKMTG 124
+F+L EV Y G+N+ + V ENV A KVR+ EN++GV LP+F+ F + + +TG
Sbjct: 67 SFALTEVKYVAGDNVKHVVLENVKEATLKVRSRTENIAGVKLPKFDHFSEGETKN-DLTG 125
Query: 125 LGRGGQQVQRAKEIYSRAVETLVELASLQTAFIILDEVIKVTNRRVNAIEHVIIPRTENT 184
L RGGQQV+ + Y +A+E LVELASLQT+F+ LDE IK TNRRVNA+E+V+ P+ ENT
Sbjct: 126 LARGGQQVRACRVAYVKAIEVLVELASLQTSFLTLDEAIKTTNRRVNALENVVKPKLENT 185
Query: 185 IAYINSELDELDREEFYRLKKVQEKKQIQTAQLDAEMKQ 223
I+YI ELDEL+RE+F+RLKK+Q K+ + + A K+
Sbjct: 186 ISYIKGELDELEREDFFRLKKIQGYKRREVERQAANAKE 224
>ECU10g0140 [C] KOG1647 Vacuolar H+-ATPase V1 sector subunit D
Length = 212
Score = 143 bits (361), Expect = 2e-34
Identities = 90/210 (42%), Positives = 123/210 (57%), Gaps = 5/210 (2%)
Query: 1 MSSNREQVFPTRMTLGLMKTKLKGANQGYSLLKRKSEALTKRFRDITKRIDDSKQKMGRV 60
M+ R VFPTRM L M+TK K A +G+SLLKRKS+AL R+R + + + +
Sbjct: 1 MTGERIPVFPTRMNLRTMETKQKSAQKGHSLLKRKSDALKVRYRAVEDEYKRKELGINQK 60
Query: 61 MQTAAFSLAEVTYATGENIGYQVQENVANARFKVRATQENVSGVYLPQFESFIDSNINDF 120
++ A F L E + G N+ + E VR+ E VSGV LP F S NI
Sbjct: 61 IRDAFFRLTEAEFL-GANLKMFLYE-CQKQNVYVRSRVEQVSGVSLPFF-SLQKENIQPI 117
Query: 121 KMTGLGRGGQQVQRAKEIYSRAVETLVELASLQTAFIILDEVIKVTNRRVNAIEHVIIPR 180
L R GQ + +E + +E LV+L +L+ +F +L+ ++ TNRRVNA+E IIPR
Sbjct: 118 LF--LDRSGQSLNECREKFLEVLEMLVDLCALKNSFRVLNSILMSTNRRVNALEFNIIPR 175
Query: 181 TENTIAYINSELDELDREEFYRLKKVQEKK 210
ENT++YI SELDE DR +F+RLKKVQ K
Sbjct: 176 LENTVSYIVSELDEQDRGDFFRLKKVQNLK 205
>7303490 [C] KOG1647 Vacuolar H+-ATPase V1 sector subunit D
Length = 373
Score = 130 bits (326), Expect = 3e-30
Identities = 84/251 (33%), Positives = 130/251 (51%), Gaps = 5/251 (1%)
Query: 8 VFPTRMTLGLMKTKLKGANQGYSLLKRKSEALTKRFRDITKRIDDSKQKMGRVMQTAAFS 67
+FP+R +MK ++ A +G LLKRK +A+ + R++ + D M+ A FS
Sbjct: 9 IFPSRANSVIMKQRVLAARRGVGLLKRKRDAIDMKLRELRRIRFDQDMHGDEAMRNAIFS 68
Query: 68 LAEVTYATGENIGYQVQENVANARFKVRATQENVSGVYLPQFESFIDSNINDFKMTGLGR 127
+A+ G + Q+ A +R T+ + GV L E + F + GL
Sbjct: 69 MAKANLL-GADFKPQMVSRSHVATVSLRRTEIKIVGVKLNTLE-LETKGVGAFPLAGLSC 126
Query: 128 GGQQVQRAKEIYSRAVETLVELASLQTAFIILDEVIKVTNRRVNAIEHVIIPRTENTIAY 187
GG QV R ++ Y++A++ LVE ASL+ +L+ TN RVNA+EHV+IP +NT Y
Sbjct: 127 GGMQVSRIRDSYTKALKALVEFASLEYQVRMLEAASLQTNMRVNALEHVVIPILQNTYNY 186
Query: 188 INSELDELDREEFYRLKKVQEKKQIQTAQLDAEMKQKKLDEANAESEGKPDSQEATKTGV 247
I EL+E +RE+FYRLK+ Q K+ +K K + + E+ K + +T
Sbjct: 187 ICGELEEFEREDFYRLKRSQAKQLEAKMAFTELIKTKNMTDEELETYIK---RNINQTRP 243
Query: 248 EAADTQEQEEQ 258
AADT +EQ
Sbjct: 244 PAADTPFDQEQ 254
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.311 0.128 0.330
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,630,062
Number of Sequences: 60738
Number of extensions: 532263
Number of successful extensions: 3930
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3911
Number of HSP's gapped (non-prelim): 8
length of query: 276
length of database: 30,389,216
effective HSP length: 105
effective length of query: 171
effective length of database: 24,011,726
effective search space: 4106005146
effective search space used: 4106005146
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)