ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI0900 good R KOG3043 General function prediction only Predicted hydrolase related to dienelactone hydrolase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI0900 304863 304120 -248
(248 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YAL049c [R] KOG3043 Predicted hydrolase related to dienelactone ... 270 1e-72
At3g23600 [R] KOG3043 Predicted hydrolase related to dienelacton... 101 1e-21
At3g23570 [R] KOG3043 Predicted hydrolase related to dienelacton... 93 3e-19
Hs20270371 [R] KOG3043 Predicted hydrolase related to dienelacto... 76 5e-14
SPAC977.15 [R] KOG3043 Predicted hydrolase related to dienelacto... 59 5e-09
SPBC30D10.14 [R] KOG3043 Predicted hydrolase related to dienelac... 59 8e-09
At1g35420 [R] KOG3043 Predicted hydrolase related to dienelacton... 55 9e-08
>YAL049c [R] KOG3043 Predicted hydrolase related to dienelactone hydrolase
Length = 246
Score = 270 bits (690), Expect = 1e-72
Identities = 136/247 (55%), Positives = 179/247 (72%), Gaps = 3/247 (1%)
Query: 1 MASNPPGKCCAQGFYHEGTPAGTTTSLYGFETYVAGPESLKNEHVLVIVTDIYGIKVNNV 60
MASN PGKCC +G H+GTP G ++G +TY AG S K E V+VI+TD+YG K NNV
Sbjct: 1 MASNQPGKCCFEGVCHDGTPKGRREEIFGLDTYAAGSTSPK-EKVIVILTDVYGNKFNNV 59
Query: 61 LLIADQLAAAGFRVYVPDFLFNDEVVALDGSFDFKSWLERHSAEKTHGLVQDFLTKLKED 120
LL AD+ A+AG+ V+VPD LF D ++ D D +W +RHS E T +V F+ LK +
Sbjct: 60 LLTADKFASAGYMVFVPDILFGD-AISSDKPIDRDAWFQRHSPEVTKKIVDGFMKLLKLE 118
Query: 121 TNPKKIGVIGHCFGAKYALLQINEKVGLADAAALAHPSFVEIEEVEAV-GKKPVLISAAE 179
+PK IGV+G+CFGAK+A+ I+ GLA+AAA+AHPSFV IEE+EA+ KKP+LISAAE
Sbjct: 119 YDPKFIGVVGYCFGAKFAVQHISGDGGLANAAAIAHPSFVSIEEIEAIDSKKPILISAAE 178
Query: 180 TDNIFPEELRYKTEEALKKQGSYYQINVFGGVSHGFAVRGDLSIPAVRYAQDKTIEDQIS 239
D+IFP LR+ TEE LK + YQ+++F GV+HGFA RGD+SIPAV+YA++K + DQI
Sbjct: 179 EDHIFPANLRHLTEEKLKDNHATYQLDLFSGVAHGFAARGDISIPAVKYAKEKVLLDQIY 238
Query: 240 FFKFFFN 246
+F F N
Sbjct: 239 WFNHFSN 245
>At3g23600 [R] KOG3043 Predicted hydrolase related to dienelactone hydrolase
Length = 239
Score = 101 bits (251), Expect = 1e-21
Identities = 67/224 (29%), Positives = 116/224 (50%), Gaps = 11/224 (4%)
Query: 21 AGTTTSLYGFETYVAGPESLKNEHVLVIVTDIYGIKVNNVLLIADQLAAAGFRVYVPDFL 80
+G L G + YV+G S +++ +++++DI+G + N+ +AD++AA+GF V VPD+
Sbjct: 19 SGHVEKLGGLDAYVSG--SAESKLCVLLISDIFGFEAPNLRALADKVAASGFYVVVPDYF 76
Query: 81 FNDEVVALDGSFDFKSWLERHSAEKTHGLVQDFLTKLKEDTNPKKIGVIGHCFGAKYALL 140
D + W++ H +K + L +K + IG G C+GAK +
Sbjct: 77 GGDPYNPSNQDRPIPVWIKDHGCDKGFENTKPVLETIK-NKGITAIGAAGMCWGAKVVVE 135
Query: 141 QINEKVGLADAAALAHPSFVEIEEVEAVGKKPVLISAAETDNIFPEELRYKTEEALKKQ- 199
E+ L AA L HPSFV +++++ GK P+ I AE D + P L + EE L +
Sbjct: 136 LSKEE--LIQAAVLLHPSFVNVDDIKG-GKAPIAILGAEIDQMSPPALLKQFEEILSSKP 192
Query: 200 --GSYYQINVFGGVSHGFAVRGDLSIPAVRYAQDKTIEDQISFF 241
SY + + VSHG+ VR ++ P A ++ ++ + +F
Sbjct: 193 EVNSY--VKIHPKVSHGWTVRYNIDEPEAVKAAEEAHKEMLDWF 234
>At3g23570 [R] KOG3043 Predicted hydrolase related to dienelactone hydrolase
Length = 240
Score = 93.2 bits (230), Expect = 3e-19
Identities = 63/221 (28%), Positives = 108/221 (48%), Gaps = 6/221 (2%)
Query: 21 AGTTTSLYGFETYVAGPESLKNEHVLVIVTDIYGIKVNNVLLIADQLAAAGFRVYVPDFL 80
+G L +TYV G S ++ +++V ++G + N+ +AD++A AGF VPDF
Sbjct: 19 SGHVEKLGNLDTYVCG--STHSKLAVLLVPHVFGYETPNLRKLADKVAEAGFYAVVPDFF 76
Query: 81 FNDEVVALDGSFDFKSWLERHSAEKTHGLVQDFLTKLKEDTNPKKIGVIGHCFGAKYALL 140
D + F W++ H EK + + LK + IG G C+GAK A+
Sbjct: 77 HGDPYNPENQDRPFPIWMKDHELEKGFEESKPIVEALK-NKGITSIGAAGFCWGAKVAVE 135
Query: 141 QINEKVGLADAAALAHPSFVEIEEVEAVGKKPVLISAAETDNIFPEELRYKTEEALKKQG 200
EK L DA L HP+ V +++++A P+ + AE D + P EL + E+ L +
Sbjct: 136 LAKEK--LVDATVLLHPARVTVDDIKAEVNLPIAVLGAEIDQVSPPELVRQFEDILASKP 193
Query: 201 SYYQ-INVFGGVSHGFAVRGDLSIPAVRYAQDKTIEDQISF 240
+ +F HG+ VR + + P+ A + +D +++
Sbjct: 194 QVKSFVKIFPRCKHGWTVRYNENDPSEVEAAMEAHKDMLAW 234
>Hs20270371 [R] KOG3043 Predicted hydrolase related to dienelactone hydrolase
Length = 245
Score = 75.9 bits (185), Expect = 5e-14
Identities = 49/175 (28%), Positives = 85/175 (48%), Gaps = 4/175 (2%)
Query: 46 LVIVTDIYGIKVNNVLLIADQLAAAGFRVYVPDFLFNDEVVALDGSFD-FKSWLERHSAE 104
++++ DI+G ++ N IAD ++ G+ VPDF E G + F WL+ +A+
Sbjct: 45 VIVIQDIFGWQLPNTRYIADMISGNGYTTIVPDFFVGQEPWDPSGDWSIFPEWLKTRNAQ 104
Query: 105 KTHGLVQDFLTKLKEDTNPKKIGVIGHCFGAKYALLQINEKVGLADAAALAHPSFVEIEE 164
K + L LK+ + +KIG++G C+G A+ + K A + + E+
Sbjct: 105 KIDREISAILKYLKQQCHAQKIGIVGFCWGGT-AVHHLMMKYSEFRAGVSVYGIVKDSED 163
Query: 165 VEAVGKKPVLISAAETDNIFP-EELRYKTEEALKKQGSYYQINVFGGVSHGFAVR 218
+ + K P L AE D + P +++ T++ + YQI F G +HGF R
Sbjct: 164 IYNL-KNPTLFIFAENDVVIPLKDVSLLTQKLKEHCKVEYQIKTFSGQTHGFVHR 217
>SPAC977.15 [R] KOG3043 Predicted hydrolase related to dienelactone hydrolase
Length = 247
Score = 59.3 bits (142), Expect = 5e-09
Identities = 68/231 (29%), Positives = 106/231 (45%), Gaps = 26/231 (11%)
Query: 29 GFETYVAGPESLKNEHVLVIVTDIYGIKVNNVLLIADQLAAAGFRVYVPDFLFNDEVVAL 88
G +TY G S K VL+ D++G+ + ADQLA +Y+PDFL N E ++
Sbjct: 29 GMQTYFTGKRSSKV--VLIGFMDVFGLS-KQIKEGADQLANHELAIYLPDFL-NGETASI 84
Query: 89 DGSFDFKSWLERHSAEK-----THGLVQDFLTKLKEDT-----NPKKIGVIGHCFGAKYA 138
+ D K+ ++ + K + L LTK+ ED KIG G C+GAK
Sbjct: 85 E-MIDPKTIEQKEARSKFMEKISSPLHWPKLTKVIEDIERIHGQDVKIGAYGFCWGAKVL 143
Query: 139 LLQINEKVGLADAAALAHPSFVEIEEVEAVGKKPVLISAAETDNIFPEEL---RYKTEEA 195
+ N++ L AHPS ++ + + V + + + D PEE+ + E +
Sbjct: 144 ITYPNKERFL--RIGCAHPSLLDPVDAKHVHCPVCFLCSKDED---PEEVDAWKKSFENS 198
Query: 196 LKKQGSYYQINVFGGVSHGF-AVRGDLSIPAVRYAQDKTIEDQISFFKFFF 245
SY++ FG + HG+ A R +LS P R D + + FFK F
Sbjct: 199 PYFSESYFE--TFGKMHHGWMAARANLSDPENRKYFDLGYQIFLKFFKELF 247
>SPBC30D10.14 [R] KOG3043 Predicted hydrolase related to dienelactone hydrolase
Length = 249
Score = 58.5 bits (140), Expect = 8e-09
Identities = 71/228 (31%), Positives = 101/228 (44%), Gaps = 25/228 (10%)
Query: 29 GFETYVAGPESLKNEHVLVIVTDIYGIKVNNVLLIADQLAAAGFRVYVPDFLFND--EVV 86
G TYV G S N VL+ DI+G+ + + AD+LA GF VY+PDFL V
Sbjct: 30 GLTTYVVG--STSNTRVLIGFMDIFGLS-DQIKEGADKLADDGFTVYLPDFLEGKPLPVT 86
Query: 87 ALDGSF--DFKSWLERHSAEKTHGLVQDFLTKLKEDTNPK-----KIGVIGHCFGAKYAL 139
AL D K + S + L L K+ E IG G C+GAK +
Sbjct: 87 ALPPKTPEDQKLCNDFFSTRISPNLHWPKLAKVVEAVRANHGPNVTIGTYGFCWGAKVLV 146
Query: 140 L--QINEKVGLADAAALAHPSFVEIEEVEAVGKKPVLISAAETDNIFPEELRYKTEEALK 197
+ VG+A HPSF + + V PVL ++ ++ ++ + EEA K
Sbjct: 147 TYPATIDFVGIAS----CHPSFPDSADAANV-HCPVLFLCSKDED---AKIIKEWEEAFK 198
Query: 198 KQGSYYQ--INVFGGVSHGF-AVRGDLSIPAVRYAQDKTIEDQISFFK 242
+Y + F + HG+ A R DLS P R D+ + SFF+
Sbjct: 199 TNPAYAKSSFETFSDMFHGWMAARADLSNPEQRKRFDEGYQKVSSFFQ 246
>At1g35420 [R] KOG3043 Predicted hydrolase related to dienelactone hydrolase
Length = 310
Score = 55.1 bits (131), Expect = 9e-08
Identities = 49/202 (24%), Positives = 85/202 (41%), Gaps = 9/202 (4%)
Query: 22 GTTTSLYGFETYVAGPESLKNEHVLVIVTDIYGIKVNNVLLIADQLAAAGFRVYVPDFLF 81
GT S+ G E Y+ N L++++D++G + + A ++A G+ V VPD
Sbjct: 87 GTEVSVDGVEGYLLTAVKNNNGTGLLLLSDVFGFQDSATRDFAYRVACNGYNVLVPDLFR 146
Query: 82 NDEVVALDGSFDFKSWLERHSAEKTHGLVQDFLTKLKEDTN----PKKIGVIGHCF-GAK 136
D +++ W H + F + E+ KK+GV+G CF G +
Sbjct: 147 GDPWSKNRPKSEYEEWRRGHDPNRIRQDTTTFTKWMVEEFAAAGISKKLGVMGFCFGGGR 206
Query: 137 YALLQINEKVGLADAAALAHPSFVEIEEVEAVGKKPVLISAAETDNIFPEELRYKTEEAL 196
+ ++ G + + ++ V K PVL A + D + + Y+ EE
Sbjct: 207 VVDVLATDESGYFSTGVSFYGTRID-SAVAGDVKVPVLFIAGDRDPLCEVKGLYEIEE-- 263
Query: 197 KKQGSYYQINVFGGVSHGFAVR 218
K G ++ V+ G HGF R
Sbjct: 264 -KIGEGSKVVVYEGRGHGFVHR 284
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.138 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,321,310
Number of Sequences: 60738
Number of extensions: 637408
Number of successful extensions: 1502
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1490
Number of HSP's gapped (non-prelim): 7
length of query: 248
length of database: 30,389,216
effective HSP length: 104
effective length of query: 144
effective length of database: 24,072,464
effective search space: 3466434816
effective search space used: 3466434816
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)