ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI0957 good E KOG3373 Amino acid transport and metabolism Glycine cleavage system H protein (lipoate-binding)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI0957 325018 324494 -175
(175 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YAL044c [E] KOG3373 Glycine cleavage system H protein (lipoate-b... 191 3e-49
SPBP19A11.01 [E] KOG3373 Glycine cleavage system H protein (lipo... 124 5e-29
At2g35120 [E] KOG3373 Glycine cleavage system H protein (lipoate... 124 9e-29
At2g35370 [E] KOG3373 Glycine cleavage system H protein (lipoate... 119 2e-27
At1g32470 [E] KOG3373 Glycine cleavage system H protein (lipoate... 111 4e-25
CE05912 [E] KOG3373 Glycine cleavage system H protein (lipoate-b... 110 8e-25
CE17610 [E] KOG3373 Glycine cleavage system H protein (lipoate-b... 110 1e-24
Hs13652471_1 [E] KOG3373 Glycine cleavage system H protein (lipo... 107 6e-24
Hs4758424_1 [E] KOG3373 Glycine cleavage system H protein (lipoa... 107 1e-23
7296410 [E] KOG3373 Glycine cleavage system H protein (lipoate-b... 96 3e-20
Hs20541772 [E] KOG3373 Glycine cleavage system H protein (lipoat... 63 2e-10
Hs22050167_1 [E] KOG3373 Glycine cleavage system H protein (lipo... 54 1e-07
>YAL044c [E] KOG3373 Glycine cleavage system H protein (lipoate-binding)
Length = 177
Score = 191 bits (486), Expect = 3e-49
Identities = 89/155 (57%), Positives = 120/155 (77%), Gaps = 1/155 (0%)
Query: 21 VSRLFLRAQSTNALNQSQLPFTFSQTGPKTVKYTTQHEWVAAHPDGSAFIGITKYAADAL 80
VS+LFLR S NALN+++LPF +S GP+ V+YT+QHEW+A H D +AF+GITKYA D+L
Sbjct: 22 VSKLFLRNSSGNALNKNKLPFLYSSQGPQAVRYTSQHEWIAVHQDKTAFVGITKYATDSL 81
Query: 81 GDATYVELPDVDTELEAGDSFGSVESVKSASEIYSPVAGTVVEVNEALSDSPQLINSDPA 140
GDATYVELP+V TE+ G+S GS+ESVKSASEIY P GTV E+N L ++P ++N DP
Sbjct: 82 GDATYVELPEVGTEISQGESLGSIESVKSASEIYQPADGTVEEINTNLEENPGVVNEDPM 141
Query: 141 GEGWIIKIKINDEADLQSDE-LLSLEQYEAHLKED 174
G+GW++K+K+ + +++ E L+SLEQYE L D
Sbjct: 142 GDGWLVKMKLGEGVNVEQVEGLMSLEQYEKTLVHD 176
>SPBP19A11.01 [E] KOG3373 Glycine cleavage system H protein (lipoate-binding)
Length = 169
Score = 124 bits (312), Expect = 5e-29
Identities = 70/174 (40%), Positives = 101/174 (57%), Gaps = 9/174 (5%)
Query: 1 MFSSSISRTAFVSSRIAKSTVSRLFLRAQSTNALNQSQLPFTFSQTGPKTVKYTTQHEWV 60
M + S R+ F+ S + A T ++ S + + T +T +HEWV
Sbjct: 1 MLARSALRSGFLPSLTSSVKTGFCLKAAAPTLSMKWSAVKYY------STKHFTKEHEWV 54
Query: 61 AAHPDGSAFIGITKYAADALGDATYVELPDVDTELEAGDSFGSVESVKSASEIYSPVAGT 120
D +GIT YAA+ALG+ +VELP+ +T + GD G+VESVKSAS++YSPV+GT
Sbjct: 55 KVDGDVGT-VGITSYAANALGEVVFVELPEPETTVSVGDGIGAVESVKSASDVYSPVSGT 113
Query: 121 VVEVNEALSDSPQLINSDPAGEGWIIKIKINDEADLQSDELLSLEQYEAHLKED 174
V +NE+L DSP ++S P EGWI KIK++ +L+S LL+ E Y KE+
Sbjct: 114 VTSINESLGDSPDKVSSSPEEEGWICKIKLSSPDELKS--LLNDESYAQFCKEE 165
>At2g35120 [E] KOG3373 Glycine cleavage system H protein (lipoate-binding)
Length = 156
Score = 124 bits (310), Expect = 9e-29
Identities = 67/152 (44%), Positives = 96/152 (63%), Gaps = 3/152 (1%)
Query: 23 RLFLRAQSTNALNQSQLPFTFSQTGPKTVKYTTQHEWVAAHPDGSAFIGITKYAADALGD 82
RLF ++ + L S FS K +KY HEWV + + F GIT +A D LGD
Sbjct: 4 RLFWASRVASHLRISVAQRGFSSVVLKDLKYADSHEWVKIDGNKATF-GITDHAQDHLGD 62
Query: 83 ATYVELPDVDTELEAGDSFGSVESVKSASEIYSPVAGTVVEVNEALSDSPQLINSDPAGE 142
YVELPDV + G SFG+VESVK+ S+I SPV+G VVEVNE L++SP L+NS P +
Sbjct: 63 VVYVELPDVGHSVSQGKSFGAVESVKATSDINSPVSGKVVEVNEELTESPGLVNSSPYEQ 122
Query: 143 GWIIKIKINDEADLQSDELLSLEQYEAHLKED 174
GWIIK++++D ++++L+ ++Y +E+
Sbjct: 123 GWIIKVELSDAG--EAEKLMDSDKYSKFCEEE 152
>At2g35370 [E] KOG3373 Glycine cleavage system H protein (lipoate-binding)
Length = 165
Score = 119 bits (298), Expect = 2e-27
Identities = 70/177 (39%), Positives = 107/177 (59%), Gaps = 23/177 (12%)
Query: 1 MFSSSISRTAFVSSRIAKSTVSRLFLRAQSTNALNQSQLPFTFSQ---TGPKTVKYTTQH 57
M++SS + +SS ++KS +S PF+FS+ T + +KY H
Sbjct: 5 MWASSTANALKLSSSVSKSHLS-----------------PFSFSRCFSTVLEGLKYANSH 47
Query: 58 EWVAAHPDGSAFIGITKYAADALGDATYVELPDVDTELEAGDSFGSVESVKSASEIYSPV 117
EWV H A IGIT +A D LG+ +VELP+ +T + SFG+VESVK+ SEI SP+
Sbjct: 48 EWVK-HEGSVATIGITAHAQDHLGEVVFVELPEDNTSVSKEKSFGAVESVKATSEILSPI 106
Query: 118 AGTVVEVNEALSDSPQLINSDPAGEGWIIKIKINDEADLQSDELLSLEQYEAHLKED 174
+G ++EVN+ L++SP LINS P +GW+IK+K + A+L+S L+ ++Y +E+
Sbjct: 107 SGEIIEVNKKLTESPGLINSSPYEDGWMIKVKPSSPAELES--LMGPKEYTKFCEEE 161
>At1g32470 [E] KOG3373 Glycine cleavage system H protein (lipoate-binding)
Length = 166
Score = 111 bits (278), Expect = 4e-25
Identities = 67/162 (41%), Positives = 99/162 (60%), Gaps = 10/162 (6%)
Query: 17 AKSTVSRLFLRAQSTNALNQSQLP-FTFSQTGPKTV---KYTTQHEWVAAHPDGSAFIGI 72
A ST + L L S++A LP F+ S+ + KY HEWV H A IGI
Sbjct: 7 ASSTANALKL---SSSASKSHLLPAFSISRCFSSVLEGLKYANSHEWVK-HEGSVATIGI 62
Query: 73 TKYAADALGDATYVELPDVDTELEAGDSFGSVESVKSASEIYSPVAGTVVEVNEALSDSP 132
T +A D LG+ +VELP+ ++ + SFG+VESVK+ SEI SP++G V+EVN L++SP
Sbjct: 63 TDHAQDHLGEVVFVELPEANSSVSKEKSFGAVESVKATSEILSPISGEVIEVNTKLTESP 122
Query: 133 QLINSDPAGEGWIIKIKINDEADLQSDELLSLEQYEAHLKED 174
LINS P +GW+IK+K + A+L++ L+ ++Y +E+
Sbjct: 123 GLINSSPYEDGWMIKVKPSSPAELEA--LMGPKEYTKFCEEE 162
>CE05912 [E] KOG3373 Glycine cleavage system H protein (lipoate-binding)
Length = 146
Score = 110 bits (276), Expect = 8e-25
Identities = 54/122 (44%), Positives = 80/122 (65%), Gaps = 3/122 (2%)
Query: 53 YTTQHEWVAAHPDGSAFIGITKYAADALGDATYVELPDVDTELEAGDSFGSVESVKSASE 112
YT +HEWV +G +GIT +A + LGD ++ELPD TE+ GDS G+VESVK+AS+
Sbjct: 24 YTKKHEWVLVE-NGVGTVGITDFATEQLGDVVFIELPDEGTEISKGDSTGAVESVKAASD 82
Query: 113 IYSPVAGTVVEVNEALSDSPQLINSDPAGEGWIIKIKINDEADLQSDELLSLEQYEAHLK 172
IY+P++GTV + N L + LIN P +GW+ K+K+ +L ++LL+ EQY K
Sbjct: 83 IYAPISGTVTQKNVKLEEEAGLINKSPFEKGWLYKLKVKSADEL--EKLLNEEQYAKFKK 140
Query: 173 ED 174
++
Sbjct: 141 DE 142
>CE17610 [E] KOG3373 Glycine cleavage system H protein (lipoate-binding)
Length = 148
Score = 110 bits (274), Expect = 1e-24
Identities = 52/122 (42%), Positives = 78/122 (63%), Gaps = 3/122 (2%)
Query: 53 YTTQHEWVAAHPDGSAFIGITKYAADALGDATYVELPDVDTELEAGDSFGSVESVKSASE 112
YT +HEW+ + +GIT +A + LGD ++ELP+ E+E GDS G+VESVK+AS+
Sbjct: 26 YTKKHEWIVVNQSVGT-VGITDFATEQLGDVVFIELPEAGVEIEKGDSTGAVESVKAASD 84
Query: 113 IYSPVAGTVVEVNEALSDSPQLINSDPAGEGWIIKIKINDEADLQSDELLSLEQYEAHLK 172
IY+PV+G ++E N L D P +IN P +GW+ +++I L +ELL+ EQY
Sbjct: 85 IYAPVSGKILEKNTKLEDEPGIINKSPLEKGWLYRLEITSNEQL--NELLTEEQYNKFKS 142
Query: 173 ED 174
E+
Sbjct: 143 EE 144
>Hs13652471_1 [E] KOG3373 Glycine cleavage system H protein (lipoate-binding)
Length = 153
Score = 107 bits (268), Expect = 6e-24
Identities = 50/111 (45%), Positives = 78/111 (70%), Gaps = 4/111 (3%)
Query: 45 QTGPKTV---KYTTQHEWVAAHPDGSAFIGITKYAADALGDATYVELPDVDTELEAGDSF 101
+TGP + K+T +HEWV +G +GI+ +A +ALGD Y LP+V T+L D F
Sbjct: 42 RTGPALLSVRKFTEKHEWVTTE-NGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEF 100
Query: 102 GSVESVKSASEIYSPVAGTVVEVNEALSDSPQLINSDPAGEGWIIKIKIND 152
G++ESVK+ASE+YSP++G V E+NEAL+++P L+N +GW+IK+ +++
Sbjct: 101 GALESVKAASELYSPLSGEVTEINEALAENPGLVNKSCCEDGWLIKMTLSN 151
>Hs4758424_1 [E] KOG3373 Glycine cleavage system H protein (lipoate-binding)
Length = 153
Score = 107 bits (266), Expect = 1e-23
Identities = 50/111 (45%), Positives = 78/111 (70%), Gaps = 4/111 (3%)
Query: 45 QTGPKTV---KYTTQHEWVAAHPDGSAFIGITKYAADALGDATYVELPDVDTELEAGDSF 101
+TGP + K+T +HEWV +G +GI+ +A +ALGD Y LP+V T+L D F
Sbjct: 42 RTGPALLSVRKFTEKHEWVTTE-NGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEF 100
Query: 102 GSVESVKSASEIYSPVAGTVVEVNEALSDSPQLINSDPAGEGWIIKIKIND 152
G++ESVK+ASE+YSP++G V E+NEAL+++P L+N +GW+IK+ +++
Sbjct: 101 GALESVKAASELYSPLSGEVTEINEALAENPGLVNKSCYEDGWLIKMTLSN 151
>7296410 [E] KOG3373 Glycine cleavage system H protein (lipoate-binding)
Length = 140
Score = 95.9 bits (237), Expect = 3e-20
Identities = 43/89 (48%), Positives = 62/89 (69%)
Query: 49 KTVKYTTQHEWVAAHPDGSAFIGITKYAADALGDATYVELPDVDTELEAGDSFGSVESVK 108
K +YT +HEWV +A +GI+ YA +ALGD + +LP+ TEL+ D G++ESVK
Sbjct: 39 KERRYTNKHEWVEVVSGSNAIVGISSYAQEALGDVVFAQLPEPGTELKQDDECGALESVK 98
Query: 109 SASEIYSPVAGTVVEVNEALSDSPQLINS 137
+ASE+YSPV+G V+E N + D+P L+NS
Sbjct: 99 AASEVYSPVSGKVIEKNAEVEDTPALVNS 127
>Hs20541772 [E] KOG3373 Glycine cleavage system H protein (lipoate-binding)
Length = 115
Score = 63.2 bits (152), Expect = 2e-10
Identities = 33/73 (45%), Positives = 46/73 (62%), Gaps = 4/73 (5%)
Query: 46 TGPKTV---KYTTQHEWVAAHPDGSAFIGITKYAADALGDATYVELPDVDTELEAGDSFG 102
TGP + K+T +HEW+ +G +GI +A +ALGD Y L +V T+L D F
Sbjct: 43 TGPVLLWVCKFTEKHEWITTE-NGIGTVGINNFANEALGDVVYCSLSEVGTKLNKQDEFC 101
Query: 103 SVESVKSASEIYS 115
++ESVK+ASEIYS
Sbjct: 102 ALESVKAASEIYS 114
>Hs22050167_1 [E] KOG3373 Glycine cleavage system H protein (lipoate-binding)
Length = 190
Score = 53.9 bits (128), Expect = 1e-07
Identities = 23/58 (39%), Positives = 42/58 (71%)
Query: 99 DSFGSVESVKSASEIYSPVAGTVVEVNEALSDSPQLINSDPAGEGWIIKIKINDEADL 156
D FG++ESVK+ E++SP++G V E+NEAL+++ +LI+ W IK+ +++ ++L
Sbjct: 62 DEFGALESVKAVHELHSPLSGEVTEINEALAENTELIDKSCYKNVWPIKMTLSNSSEL 119
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.310 0.126 0.345
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,458,796
Number of Sequences: 60738
Number of extensions: 358821
Number of successful extensions: 794
Number of sequences better than 1.0e-05: 12
Number of HSP's better than 0.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 779
Number of HSP's gapped (non-prelim): 12
length of query: 175
length of database: 30,389,216
effective HSP length: 99
effective length of query: 76
effective length of database: 24,376,154
effective search space: 1852587704
effective search space used: 1852587704
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)