ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactI0974    suspect: LH   JT    KOG3791  Signal transduction mechanisms                                 Predicted RNA-binding protein involved in translational regulation
r_klactI0974    suspect: LH   JT    KOG3791  Translation, ribosomal structure and biogenesis                Predicted RNA-binding protein involved in translational regulation

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactI0974  330186 328810 -459 
         (459 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YOR359w [JT] KOG3791 Predicted RNA-binding protein involved in t...   251   2e-66
SPBC13E7.03c [JT] KOG3791 Predicted RNA-binding protein involved...    65   2e-10

>YOR359w [JT] KOG3791 Predicted RNA-binding protein involved in
           translational regulation
          Length = 523

 Score =  251 bits (641), Expect = 2e-66
 Identities = 188/537 (35%), Positives = 238/537 (44%), Gaps = 110/537 (20%)

Query: 11  PGRTQSPVNLPRGAHPGAVLLSPKGSSANLQALGTNPTSPSXXXXXXXXXXXXXXXXXXX 70
           P  ++SP N+ RGAHPGAVLLSP+ S+ N      NP S S                   
Sbjct: 9   PTGSKSPYNMSRGAHPGAVLLSPQSSAINKN----NPGSNSGNNQGNSSVTANVLSPQSH 64

Query: 71  XXHLRDDLGRPSNM-------------------------------------------VSP 87
              L D L + S M                                           VSP
Sbjct: 65  SMSLNDMLDQQSFMLDTAGTRAQPLQQQQQQQQQQQQASLPSLNIQTVSSTAAGSAIVSP 124

Query: 88  LSNS--AMNPTASNGSTFLDTFSR-PTSSMAINHQ----PLPL--------QHSNSNANV 132
           +  S  A+  T S+ S +LD+F R P + + I  Q    PLP         Q   ++ N+
Sbjct: 125 MMQSPKALQSTLSSTSMYLDSFQRSPNNILGIPSQSGSIPLPQSRQSQQQSQSQKNDPNM 184

Query: 133 MVNFTNDVNQLCNWISMLTPSQQNTVMDNLLSSLNEEVLHSTKLKLDSLVHSGYISPQLG 192
             NF+ D+NQLC+WISML  SQQNTVMDN+LS LN++VL  TKLK+++L ++ +ISP L 
Sbjct: 185 GTNFSQDINQLCSWISMLNSSQQNTVMDNILSILNDDVLKYTKLKIETLTNTPFISPPLP 244

Query: 193 PIASPIPNKVQSQVHQAENPQPLNLDSVL----------NGDN-IYRQWSPLPQNTASPM 241
            IASPIPN+  +Q+        LN+DSV           N DN +Y+ WSP P +   P+
Sbjct: 245 AIASPIPNRDDTQI--------LNIDSVFSSSPITNDPENTDNLLYQNWSPQPHSI--PI 294

Query: 242 QQPMYDYLADI-PRPRSADPYNAFKKXXXXXXXXXXXXXXXXXXXXXXXXXPFGXXXXXX 300
            QP+YD + D   R +SA+P+                              P        
Sbjct: 295 SQPIYDNITDASQRSKSAEPH-----VNSSPNLIPVQKQFNNGNSTKYKKLPSENPNYLS 349

Query: 301 XXXXXXXXXXXXXGRKNMNQ--NQHQNQYLNRPQSPT----------------NIKANVF 342
                         R NM    N H +  L+ P   T                    NVF
Sbjct: 350 HSLSSSHSFFQPKKRSNMGNEYNSHHHHSLHHPLHNTTSYFSNTSRPSGTDLNKSNQNVF 409

Query: 343 NKTSLATSSSNSGXXXXXXXXXXXXXXXXXXXXXXXXXKGLCDPKLLKNVPAWLKSLRLH 402
           N T    +  N+G                         K L DPKLLKN+P WLKSLRLH
Sbjct: 410 NNT---ITHPNAGPTSATSTSTSSNGNTPLSSNSSMNPKSLTDPKLLKNIPMWLKSLRLH 466

Query: 403 KYSEALGSKPWFELIYLDDEALENMGVSALGARRKLLKAFSIVREYKANDMIDSSAF 459
           KYS+AL   PW ELIYLDDE LE  GV ALGARRKLLKAF IV +YK  D+ID SA+
Sbjct: 467 KYSDALSGTPWIELIYLDDETLEKKGVLALGARRKLLKAFGIVIDYKERDLIDRSAY 523


>SPBC13E7.03c [JT] KOG3791 Predicted RNA-binding protein involved in
           translational regulation
          Length = 713

 Score = 65.5 bits (158), Expect = 2e-10
 Identities = 31/59 (52%), Positives = 42/59 (70%)

Query: 387 KLLKNVPAWLKSLRLHKYSEALGSKPWFELIYLDDEALENMGVSALGARRKLLKAFSIV 445
           +L +++P+WL+SLRLHKY+  L    W  L+ L D  L+N G+ ALGARRKLLK+F  V
Sbjct: 595 ELPQDIPSWLRSLRLHKYTNNLKDTDWDALVSLSDLDLQNRGIMALGARRKLLKSFQEV 653


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.312    0.128    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,081,155
Number of Sequences: 60738
Number of extensions: 911040
Number of successful extensions: 2214
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2207
Number of HSP's gapped (non-prelim): 4
length of query: 459
length of database: 30,389,216
effective HSP length: 110
effective length of query: 349
effective length of database: 23,708,036
effective search space: 8274104564
effective search space used: 8274104564
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)