ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI1113 good S KOG2936 Function unknown Uncharacterized conserved protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI1113 375985 374900 -362
(362 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR214w [S] KOG2936 Uncharacterized conserved protein 391 e-109
SPBC1711.08 [S] KOG2936 Uncharacterized conserved protein 248 1e-65
7302133 [S] KOG2936 Uncharacterized conserved protein 138 1e-32
Hs6912280 [S] KOG2936 Uncharacterized conserved protein 125 7e-29
CE07817 [S] KOG2936 Uncharacterized conserved protein 107 4e-23
YNL281w [S] KOG2936 Uncharacterized conserved protein 100 4e-21
At3g12050 [S] KOG2936 Uncharacterized conserved protein 98 2e-20
Hs17441336 [S] KOG2936 Uncharacterized conserved protein 51 3e-06
Hs22045341 [S] KOG2936 Uncharacterized conserved protein 50 7e-06
>YDR214w [S] KOG2936 Uncharacterized conserved protein
Length = 350
Score = 391 bits (1005), Expect = e-109
Identities = 202/369 (54%), Positives = 249/369 (66%), Gaps = 26/369 (7%)
Query: 1 MVVHNPNNWHWVDKNCIDWAKKYFQEKLVGLTTGESTDELYAEITKLSSLEGDCEVNQRK 60
MVV+NPNNWHWVDKNCI WAK+YF++KLVG+ G D+ YA+I +SS+EGDCEVNQRK
Sbjct: 1 MVVNNPNNWHWVDKNCIGWAKEYFKQKLVGVEAGSVKDKKYAKIKSVSSIEGDCEVNQRK 60
Query: 61 GKVISLFDLKLNVGIAGHVGENK-----LEGSITVPEVAFDSDPEDYQFDISIFKETSKL 115
GKVISLFDLK+ V I GHV EGSI VPEVAFDS+ YQFDISIFKETS+L
Sbjct: 61 GKVISLFDLKITVLIEGHVDSKDGSALPFEGSINVPEVAFDSEASSYQFDISIFKETSEL 120
Query: 116 FEIKPVIREKLIPQLRDIFSNFGKQLLLTHGNDIQVSSDKVTSKFTKANQQSSFSTSGST 175
E KP+IR +L+P+LR IF FGK LL THGNDIQV +V S +T+ NQ+SSF+
Sbjct: 121 SEAKPLIRSELLPKLRQIFQQFGKDLLATHGNDIQVPESQVKSNYTRGNQKSSFT---EI 177
Query: 176 KEDANKDXXXXXXXXXXXXXXXXXXXXXXETQQKNSGNAAKYNTTKVHLESSFQVPAIEL 235
K+ A+K + Q SGN+ T ++LE +F VP+ EL
Sbjct: 178 KDSASKPKKNALPSSTSTSAPVSSTN---KVPQNGSGNS-----TSIYLEPTFNVPSSEL 229
Query: 236 YNTFIDKRRIEAWSRSPIQPEANTGDF--SGSTLQLNDAFKLFGGNITMKFLSGEPGKKI 293
Y TF+DK+RI AW+RS F SG L+ + F+LFGGN+ + +S E KK+
Sbjct: 230 YETFLDKQRILAWTRS--------AQFFNSGPKLETKEKFELFGGNVISELVSCEKDKKL 281
Query: 294 VFQWRLNDWKQGHYSELSLEFHESQEYHETKLVVNWSGIPVGEEDRVRGNFEEYYIRSIK 353
VF W+L DW S + + FHESQE+HETKL V W+GIPVGEEDRVR NFEEYY+RSIK
Sbjct: 282 VFHWKLKDWSAPFNSTIEMTFHESQEFHETKLQVKWTGIPVGEEDRVRANFEEYYVRSIK 341
Query: 354 VTFGFGAIL 362
+TFGFGA+L
Sbjct: 342 LTFGFGAVL 350
>SPBC1711.08 [S] KOG2936 Uncharacterized conserved protein
Length = 336
Score = 248 bits (632), Expect = 1e-65
Identities = 134/360 (37%), Positives = 202/360 (55%), Gaps = 32/360 (8%)
Query: 5 NPNNWHWVDKNCIDWAKKYFQEKLVGLTTGESTDELYAEITKLSSLEGDCEVNQRKGKVI 64
NPNNWHW K+C W+ +YF ++L + E A IT+++S EGD +V+ RK KVI
Sbjct: 7 NPNNWHWTSKDCRVWSHEYFNKELPKIQASEGPTS--ARITQVNSCEGDVDVSMRKRKVI 64
Query: 65 SLFDLKLNVGIAGHVGEN-KLEGSITVPEVAFDSDPEDYQFDISIFKETSKLFEIKPVIR 123
++FDLK+ + G + + GSIT PE+++D DY FDI I+ + + IK ++R
Sbjct: 65 TIFDLKIQMEFKGETKDGVEATGSITCPELSYDLGYSDYVFDIDIYSASKEKEPIKELVR 124
Query: 124 EKLIPQLRDIFSNFGKQLLLTHGNDIQVSSDKVTSKFTKA-NQQSSFSTSGSTKEDANKD 182
EK+IPQ+R +FS F + LL THG+D+ +S+++ + SSF + S++ +NK
Sbjct: 125 EKIIPQIRQLFSGFSQVLLQTHGDDVYLSTEEHNGNAARGLPVHSSFKQNNSSQTSSNKG 184
Query: 183 XXXXXXXXXXXXXXXXXXXXXXETQQKNSGNAAKYNTTKVHLESSFQVPAIELYNTFIDK 242
+ S +A NT + +F PA ELY TF+D
Sbjct: 185 TTTVAAG----------------SGSDGSRVSAVVNTADISENYTFDAPANELYATFLDP 228
Query: 243 RRIEAWSRSPIQPEANTGDFSGSTLQLNDAFKLFGGNITMKFLSGEPGKKIVFQWRLNDW 302
R+ AWSR+P Q + ++ AF LF GN+ KFL E KKIV WRL+ W
Sbjct: 229 ARVAAWSRAPPQLD----------VRPQGAFSLFHGNVVGKFLVLEENKKIVQTWRLSSW 278
Query: 303 KQGHYSELSLEFHESQEYHETKLVVNWSGIPVGEEDRVRGNFEEYYIRSIKVTFGFGAIL 362
GHY+E++ F ++ Y ++++ G+P+GEE+ V+GN ++YYIR IK FGFGA+L
Sbjct: 279 PTGHYAEITFTFDQADSYTTLRMIM--KGVPIGEEEVVQGNIQDYYIRPIKTVFGFGAVL 336
>7302133 [S] KOG2936 Uncharacterized conserved protein
Length = 361
Score = 138 bits (348), Expect = 1e-32
Identities = 96/371 (25%), Positives = 162/371 (42%), Gaps = 53/371 (14%)
Query: 5 NPNNWHWVDKNCIDWAKKYFQEKLVGLTTGE-----STDELYAEITKLSSLEGDCEVNQR 59
N NNWHW +KN W+K + GE ++ + + G+ VN R
Sbjct: 21 NVNNWHWTEKNATPWSKDRLHQLFQDFKIGEYIRICRQSDIECAVDSVDKCSGEATVNNR 80
Query: 60 KGKVISLFDLKLNVGIAGHVGENKL---EGSITVPEVAFDSDPEDYQFDISIFK-----E 111
KGK+I ++ +L + +G + +N +G +T+P ++ +++ D + ++I + E
Sbjct: 81 KGKLIFFYEWELVLKWSGKLLKNSKLIHKGKLTIPNLSEENELADVEITVTIDESNDESE 140
Query: 112 TSKLFEI---KPVIREKLIPQLRDIFSNFGKQLLLTHGNDIQVSSDKVTSKFTKANQQSS 168
T K F + +R++L +R++ + K L+L D + N ++
Sbjct: 141 TLKQFMYNVGRDRVRQQLASYIRELKEEYSKNLILPKKGD----------EAGAGNTVAN 190
Query: 169 FSTSGSTKEDANKDXXXXXXXXXXXXXXXXXXXXXXETQQKNSGNAAKYNTTKVHLESSF 228
F + +T+ A + KNSG K + + + F
Sbjct: 191 FKDANNTRNAAQN---------------IALNSSVAAPRLKNSGIGCKLDVRTLSMTEEF 235
Query: 229 QVPAIELYNTFIDKRRIEAWSRSPIQPEANTGDFSGSTLQLNDAFKLFGGNITMKFLSGE 288
A +LYN + A++R+P + +A G F L+GGN+ KF
Sbjct: 236 HCSANDLYNALTKPEMVTAFTRAPAKVDAVRGG----------EFILYGGNVLGKFEELV 285
Query: 289 PGKKIVFQWRLNDWKQGHYSELSLEFHESQEYHETKLVVNWSGIPVGEEDRVRGNFEEYY 348
P KKI WRL +W GHYS + +E E+ T + + +GIP E D ++ N+ YY
Sbjct: 286 PEKKIQQSWRLKNWTSGHYSNVVIELEETSS--STMMSLKQTGIPASEFDAMKTNWYRYY 343
Query: 349 IRSIKVTFGFG 359
SIK TFGFG
Sbjct: 344 WHSIKQTFGFG 354
>Hs6912280 [S] KOG2936 Uncharacterized conserved protein
Length = 338
Score = 125 bits (315), Expect = 7e-29
Identities = 88/359 (24%), Positives = 162/359 (44%), Gaps = 43/359 (11%)
Query: 5 NPNNWHWVDKNCIDWAKKYFQEKLVGLTTGESTDELYAEITKLSSLEGDCEVNQRKGKVI 64
N NNWHW +++ +W+ K + L +E E+T++S L+G+ +N RKGK+I
Sbjct: 21 NVNNWHWTERDASNWSTDKL--KTLFLAVQVQNEEGKCEVTEVSKLDGEASINNRKGKLI 78
Query: 65 SLFDLKLNVGIAGHVGEN-KLEGSITVPEVAFDSDPEDYQFDISIFKETSKLFEIKPVIR 123
++ + + G + +G + +P ++ ++ ++ + +S+ K+ + +++
Sbjct: 79 FFYEWSVKLNWTGTSKSGVQYKGHVEIPNLSDENSVDEVEISVSLAKDEPDT-NLVALMK 137
Query: 124 EKLIPQLRDIFSNFGKQLLLTHGNDIQVSSDKVTSKFTKANQQSSFSTSGSTKEDANKDX 183
E+ + LR+ I +S+ K ++FT+ G N +
Sbjct: 138 EEGVKLLREAMG-------------IYISTLK--TEFTQ----------GMILPTMNGES 172
Query: 184 XXXXXXXXXXXXXXXXXXXXXETQQKNSGNAAKYNTTKVHLESSFQVPAIELYNTFIDKR 243
+TQ + G K T K+ L+ +F ELY F +
Sbjct: 173 VDPVGQPALKTEERKAKPAPSKTQARPVG--VKIPTCKITLKETFLTSPEELYRVFTTQE 230
Query: 244 RIEAWSRSPIQPEANTGDFSGSTLQLNDAFKLFGGNITMKFLSGEPGKKIVFQWRLNDWK 303
++A++ +P EA+ G F + GN++ +F P K IV +WR W
Sbjct: 231 LVQAFTHAPATLEADRGG----------KFHMVDGNVSGEFTDLVPEKHIVMKWRFKSWP 280
Query: 304 QGHYSELSLEFHESQEYHETKLVVNWSGIPVGEEDRVRGNFEEYYIRSIKVTFGFGAIL 362
+GH++ ++L F + ET+L + GIP EE+R R ++ YY IK TFG+GA L
Sbjct: 281 EGHFATITLTFIDKNG--ETELCMEGRGIPAPEEERTRQGWQRYYFEGIKQTFGYGARL 337
>CE07817 [S] KOG2936 Uncharacterized conserved protein
Length = 342
Score = 107 bits (266), Expect = 4e-23
Identities = 73/356 (20%), Positives = 153/356 (42%), Gaps = 40/356 (11%)
Query: 5 NPNNWHWVDKNCIDWAKKYFQEKLVGLTTGESTDELYAEITKLSSLEGDCEVNQRKGKVI 64
N NNWHW +KN W+ +E L G ++ + + I ++ ++G+ N RK K+I
Sbjct: 21 NVNNWHWSEKNATPWSLNRLRELLTGFSSEDG--PIVVTIDEIKKIDGEATANNRKAKLI 78
Query: 65 SLFDLKLNVGIAGHV--GENKLEGSITVPEVAFDSDPEDYQFDISIFKETSKLFEIKPVI 122
LF+ + V E++ +G+ +P ++ ++D + + S+ +I+ V+
Sbjct: 79 FLFEWVIEGTFIARVSGSEDEYKGTFDIPNLSDENDASEVDVNSSLSGNGPMAHQIRQVL 138
Query: 123 REKLIPQLRDIFSNFGKQLLLTHGNDIQVSSDKVTSKFTKANQQSSFSTSGSTKEDANKD 182
+ I +++D+ + ++L + + +DKV + T+G T +
Sbjct: 139 NKSFIAKIQDVMGIYIRELKEEFSKGLILPTDKVKPQVV---------TTGKTSVVDKRQ 189
Query: 183 XXXXXXXXXXXXXXXXXXXXXXETQQKNSGNAAKYNTTKVHLESSFQVPAIELYNTFIDK 242
E SGN ++T +V +++ ++ +
Sbjct: 190 ---------------FQNTVVAEKDASTSGNEV-FSTKEVSTSDTYKATPDRVFEALTET 233
Query: 243 RRIEAWSRSPIQPEANTGDFSGSTLQLNDAFKLFGGNITMKFLSGEPGKKIVFQWRLNDW 302
+ + W+ + I G+++ + +F LFG N+T F EP K+IV +WRL +
Sbjct: 234 QFVRGWTNNSI------GEWN---FKEGGSFALFGENVTGTFEKIEPNKEIVKKWRLKKY 284
Query: 303 KQGHYSELSLEFHESQEYHETKLVVNWSGIPVGEEDRVRGNFEEYYIRSIKVTFGF 358
H++ + + ++ + K++ +P + + + YY+ SI TFGF
Sbjct: 285 PNNHHATIHFQLKDTGSGTDIKIIA--KDVPTHLAEETQQGLDRYYLSSIGRTFGF 338
>YNL281w [S] KOG2936 Uncharacterized conserved protein
Length = 153
Score = 100 bits (248), Expect = 4e-21
Identities = 57/161 (35%), Positives = 87/161 (53%), Gaps = 18/161 (11%)
Query: 1 MVVHNPNNWHWVDKNCIDWAKKYFQEKLVGLTTGESTDELYAEITKLSSLEGDCEVNQRK 60
MVV NPNNWHWVDKN + W+K Y KL L+T S + E+T++SS+ GD V+QRK
Sbjct: 1 MVVLNPNNWHWVDKNTLPWSKDYLNGKLTSLSTVSSDGKSKIELTQVSSITGDSNVSQRK 60
Query: 61 GKVISLFDLKLNVGI-AGHVGENK---------LEGSITVPEVAFDSDPEDYQFDISIFK 110
GK I FDL+L++ + ++ NK +G + +PE D + DI I
Sbjct: 61 GKPICYFDLQLSMNVKVTNLDTNKDDEDDDGILADGKLEIPEFMHD------ESDIPILS 114
Query: 111 ETSKLFEIKPVIREKLIPQLRDIFSNFGKQLLLTHGNDIQV 151
+ F+ ++R + +P++ + + L+ H DIQV
Sbjct: 115 QGFDAFD--GLVRSEFVPKVVETLLKYQDDLIKEHSKDIQV 153
>At3g12050 [S] KOG2936 Uncharacterized conserved protein
Length = 360
Score = 97.8 bits (242), Expect = 2e-20
Identities = 81/374 (21%), Positives = 150/374 (39%), Gaps = 54/374 (14%)
Query: 5 NPNNWHWVDKNCIDWAKKYFQEKLVGLTTGESTDELYAEITKLSSLEGDCEVNQRKGKVI 64
N +NWHW + NC++W++ +F ++ G+ L+ +++K+ +EG+ VN RKGK+I
Sbjct: 21 NVHNWHWSETNCLEWSRNFFTKQFSGVDILSGEGNLFIKVSKIEKVEGEAYVNVRKGKII 80
Query: 65 SLFDLKLNVGIAGHVGEN------KLEGSITVPEVAFDSDPEDYQFDISIFKETSKLFEI 118
++L +++ G ++ K +G + +P ++ ++ ED + S+ E
Sbjct: 81 PGYELNVSLSWEGEAKDSDGKTLLKADGLVDMPYISDENADEDPEIRFSVKDE------- 133
Query: 119 KPVIREKLIPQLRDIFSNFGKQLLLTHGNDIQVSSDKVTSKFTKANQQSSFSTSGSTKED 178
P+ R L++ GK+++L +KV + +K+
Sbjct: 134 GPIGR-----TLKEAMVKKGKEIIL----------EKVRVYVEAMARGGPCRDELESKKV 178
Query: 179 ANKDXXXXXXXXXXXXXXXXXXXXXXETQQKNSGNAAKYNTTK----VHLESSFQVPAIE 234
A K + K K T + + + F A +
Sbjct: 179 APKSVAAGSATVAVEKSGAAPVVSAAAVESKVVKEKKKAKTKEGFKTITMTEKFNCRARD 238
Query: 235 LYNTFIDKRRIEAWSRSPIQPEANTGDFSGSTLQLNDAFKLFGGNITMKFLSGEPGKKIV 294
LY +D+ R + +++S + + +N +F G++T L E GK IV
Sbjct: 239 LYEILMDENRWKGFTQSNAK----------ISKDVNGPISVFDGSVTGMNLELEEGKLIV 288
Query: 295 FQWRLNDWKQGHYSELSLEFHESQEYHETKLVVNWSGIPVGEEDR---------VRGNFE 345
+WR W G S + + F E Q +VN + V EEDR +
Sbjct: 289 QKWRFGSWPDGLDSTVKIVFEEPQ---PGVTIVNLTHTDVPEEDRYGNATVVENTERGWR 345
Query: 346 EYYIRSIKVTFGFG 359
+ I+ FGFG
Sbjct: 346 DLIFHRIRAVFGFG 359
>Hs17441336 [S] KOG2936 Uncharacterized conserved protein
Length = 257
Score = 50.8 bits (120), Expect = 3e-06
Identities = 31/130 (23%), Positives = 62/130 (46%), Gaps = 26/130 (20%)
Query: 224 LESSFQVPAIELYNTFIDKRRIEAWSRSPIQPEANTGDFSGSTLQLNDAFKLFGGNITMK 283
+ + F V +LY+ F K ++ +S+S EA G F++F GNIT +
Sbjct: 150 MRAQFDVTVEQLYSIFTMKDLVQKFSKSSAVLEAEKGG----------KFQMFVGNITDE 199
Query: 284 FLSGEPGKKIVFQWRLNDWKQGHYSELSLEFHESQEYHETKLVVNWSGIPVGEEDRVRGN 343
++ KKI+ +WR +W +G ++ L+F + G+P+ +E+ ++
Sbjct: 200 YIESLTNKKIIMKWRCRNWPEG---QMELQF-------------DCKGVPICKEENMKFC 243
Query: 344 FEEYYIRSIK 353
+++ + IK
Sbjct: 244 WQKQHFEEIK 253
>Hs22045341 [S] KOG2936 Uncharacterized conserved protein
Length = 127
Score = 49.7 bits (117), Expect = 7e-06
Identities = 26/87 (29%), Positives = 43/87 (48%), Gaps = 10/87 (11%)
Query: 228 FQVPAIELYNTFIDKRRIEAWSRSPIQPEANTGDFSGSTLQLNDAFKLFGGNITMKFLSG 287
F +LY+ F K ++ +S+S E G F++F GNIT ++L
Sbjct: 5 FDTTVEQLYSIFTVKELVQKFSKSTAVLETEKGG----------KFQMFDGNITGEYLGL 54
Query: 288 EPGKKIVFQWRLNDWKQGHYSELSLEF 314
KKI+ +WR +W + HY+ ++L F
Sbjct: 55 LTNKKIIMKWRCGNWPEEHYAMVALNF 81
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.314 0.133 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,067,680
Number of Sequences: 60738
Number of extensions: 872369
Number of successful extensions: 1657
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1633
Number of HSP's gapped (non-prelim): 12
length of query: 362
length of database: 30,389,216
effective HSP length: 108
effective length of query: 254
effective length of database: 23,829,512
effective search space: 6052696048
effective search space used: 6052696048
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)