ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI1319 good S KOG3292 Function unknown Predicted membrane protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI1319 450111 449593 -173
(173 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores:
Score E
Sequences producing significant alignments: (bits) Value
YGL010w [S] KOG3292 Predicted membrane protein 180 1e-45
SPAC16E8.02 [S] KOG3292 Predicted membrane protein 110 7e-25
At1g18720 [S] KOG3292 Predicted membrane protein 100 2e-21
At1g74440 [S] KOG3292 Predicted membrane protein 97 1e-20
>YGL010w [S] KOG3292 Predicted membrane protein
Length = 174
Score = 180 bits (456), Expect = 1e-45
Identities = 93/173 (53%), Positives = 121/173 (69%)
Query: 1 MSPSLLDLETQLAFYKAYHNNTGNVLIHSIFVPTILFTSMRILNDVKIWNGYTLTHLLAL 60
M LLDL +QL FYK YH+N NVLIHSIFVPTILF+ +L+ VKI+ +LT +L++
Sbjct: 1 MGEGLLDLRSQLGFYKFYHHNPKNVLIHSIFVPTILFSGSCMLHRVKIYQSISLTAVLSV 60
Query: 61 SFGLFYILLRVGTGILASLILALTCKFLNDGTIKMTQTTAWILFVISWIFQFIGHGVFEK 120
F +FY LL + TG+LA ++L L L D + +T LF I WIFQF+GHGVFEK
Sbjct: 61 LFSIFYCLLYLPTGLLAGVLLLLLNLALIDHRVDLTFKQELGLFTIGWIFQFVGHGVFEK 120
Query: 121 RKPALLDNLVQSLVLAPYFILYELLFILGFMPELKKNLQARVDKELDRMRASK 173
R+PAL+DNLVQSLVLAPYFI++E LF LGFMP LK L+ ++ + +R +
Sbjct: 121 RRPALIDNLVQSLVLAPYFIMFEFLFKLGFMPRLKATLEHDLEIKQRNLRMQR 173
>SPAC16E8.02 [S] KOG3292 Predicted membrane protein
Length = 222
Score = 110 bits (276), Expect = 7e-25
Identities = 61/165 (36%), Positives = 95/165 (56%), Gaps = 10/165 (6%)
Query: 8 LETQLAFYKAYHNNTGNVLIHSIFVPTILFTSMRILND--VKIWNG---YTLTHLLALSF 62
L +FY AYH+N N+ IH + +P +L T++ +L++ + + N + HL+ L++
Sbjct: 4 LSRSYSFYAAYHSNPVNIKIHQVCIPLLLLTALVLLHNFVITLINSKLQINVAHLVGLAY 63
Query: 63 GLFYILLRVGTGILASLIL-----ALTCKFLNDGTIKMTQTTAWILFVISWIFQFIGHGV 117
+FY+ L G+L S +L L K + + +A ++ VI WI QFIGHGV
Sbjct: 64 QIFYVTLDPLDGLLYSPVLYLFSYILPSKLFTIFSRSLVNRSAAVVHVICWILQFIGHGV 123
Query: 118 FEKRKPALLDNLVQSLVLAPYFILYELLFILGFMPELKKNLQARV 162
FEKRKPALLDNL+QSL +AP F E +G+ P + ++A +
Sbjct: 124 FEKRKPALLDNLIQSLFIAPLFAFLETGPFVGYYPSVVSKIRANI 168
>At1g18720 [S] KOG3292 Predicted membrane protein
Length = 206
Score = 99.8 bits (247), Expect = 2e-21
Identities = 68/195 (34%), Positives = 107/195 (54%), Gaps = 28/195 (14%)
Query: 5 LLDLETQLAFYKAYHNNTGNVLIHSIFVPTILFTSMRILNDV-------KIWNGYTLT-- 55
L DLE AFY AYH+N N+LIH IFV I F+ + +L+ ++ +LT
Sbjct: 7 LFDLEKHFAFYGAYHSNPINILIHIIFVWPIFFSVLLLLHSSTPIFDPSQLGFSQSLTLD 66
Query: 56 --------HLLALSFGLFYILLRVGTGILASLILALTCKFLNDGTIKMTQTTAWILFV-- 105
+ AL + LFYI L +G +A+L + +C ++ + + ++ L V
Sbjct: 67 GVLRFNVGFIFALIYALFYIGLDKKSGFVAAL-MCFSC-WVGSSFLAVRLGSSLALKVGL 124
Query: 106 ----ISWIFQFIGHGVFEKRKPALLDNLVQSLVLAPYFILYELL-FILGF--MPELKKNL 158
+ W QF+GHGVFEKR PALLDNL+Q+ ++AP+F+L E+L + G+ P + +
Sbjct: 125 ASQLLCWTGQFVGHGVFEKRAPALLDNLLQAFLMAPFFVLLEVLQSVFGYEPYPGFQARV 184
Query: 159 QARVDKELDRMRASK 173
A+V+ ++ RA K
Sbjct: 185 NAKVESDIKEYRAKK 199
>At1g74440 [S] KOG3292 Predicted membrane protein
Length = 208
Score = 96.7 bits (239), Expect = 1e-20
Identities = 64/189 (33%), Positives = 103/189 (53%), Gaps = 32/189 (16%)
Query: 5 LLDLETQLAFYKAYHNNTGNVLIHSIFV-PTILFTSMRILNDVKIWNGYTLTHLLALSF- 62
LLDLE AFY AYH+N N++IH++FV P + T + + + I + L L +L+F
Sbjct: 7 LLDLEKHFAFYGAYHSNPINIIIHTLFVWPNVFATLLFLYSTPPILDHSQLGFLKSLTFD 66
Query: 63 ---------------GLFYILLRVGTGILASLILA--------LTCKFLNDGTIKMTQTT 99
+FYI L +G+LA+L+ L + + T+K+ +
Sbjct: 67 GVLRLDIGFTLTVTYAVFYICLDKKSGVLAALLCFSCWIGSSFLAARLGHSLTLKVGVAS 126
Query: 100 AWILFVISWIFQFIGHGVFEKRKPALLDNLVQSLVLAPYFILYELL-FILGFMPELKKNL 158
++ W QF+GHG+FEKR PALLDNL+Q+ ++ P+F+L E+L + G+ P
Sbjct: 127 Q----LLCWTGQFLGHGLFEKRAPALLDNLLQAFLMGPFFVLLEVLQSVFGYEP--YPGF 180
Query: 159 QARVDKELD 167
+ARVD +++
Sbjct: 181 KARVDSKIE 189
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.330 0.144 0.432
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,860,492
Number of Sequences: 60738
Number of extensions: 373148
Number of successful extensions: 1085
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1076
Number of HSP's gapped (non-prelim): 4
length of query: 173
length of database: 30,389,216
effective HSP length: 99
effective length of query: 74
effective length of database: 24,376,154
effective search space: 1803835396
effective search space used: 1803835396
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)