ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI1507 good I KOG2822 Lipid transport and metabolism Sphingoid base-phosphate phosphatase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI1507 515641 514937 -235
(235 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKR053c [I] KOG2822 Sphingoid base-phosphate phosphatase 167 2e-41
YJL134w [I] KOG2822 Sphingoid base-phosphate phosphatase 157 9e-39
SPAC823.11 [I] KOG2822 Sphingoid base-phosphate phosphatase 99 4e-21
At3g58490 [I] KOG2822 Sphingoid base-phosphate phosphatase 60 3e-09
>YKR053c [I] KOG2822 Sphingoid base-phosphate phosphatase
Length = 404
Score = 167 bits (422), Expect = 2e-41
Identities = 91/224 (40%), Positives = 133/224 (58%), Gaps = 15/224 (6%)
Query: 12 VFENYLYIMTALYFFILTL--GRIYCGMHGILDITSGITIGIFCCAIRVFTNSYLDFDKF 69
V+ L +++ + F+ LTL GR+YCGMHG+LD+ SG +G C IR++ L F
Sbjct: 183 VWPTKLLLLSLVIFYYLTLVFGRVYCGMHGMLDLFSGAAVGAICFFIRIWVVHALR--NF 240
Query: 70 TIQSGVWYPILSIITGAALLKFHARPIDECPCIEDSVAFIGVIMGIECSDWLSPRVYDSE 129
I +W+P+LS+ G +L H RPIDECPC EDSVAFIGV+ G++CSDWL+ R
Sbjct: 241 QIGEHLWFPLLSVAWGLFILFNHVRPIDECPCFEDSVAFIGVVSGLDCSDWLTER----- 295
Query: 130 LYVMPFSYSRIGITGT--VLRIIVGILAVLTWK-TLSKKVIYWFLSKTI--GDDRPFTKE 184
Y SR G+ LR +VG+ +V+ WK +SK +Y L K + DDR
Sbjct: 296 -YGWNLVCSRYASCGSKVFLRPLVGVASVIVWKDVISKTAVYTLLIKLLRFHDDRSEKVH 354
Query: 185 ELKQHQESAEVRLFTPLANIDVIGRSIIYAGIPATVILICPLLY 228
+ E E L++ ++ ++++GR +IYAGIP TV L+CP+ +
Sbjct: 355 FHNETSEEEECLLYSGVSKVEIVGRFLIYAGIPTTVFLLCPVFF 398
>YJL134w [I] KOG2822 Sphingoid base-phosphate phosphatase
Length = 409
Score = 157 bits (398), Expect = 9e-39
Identities = 91/225 (40%), Positives = 129/225 (56%), Gaps = 13/225 (5%)
Query: 17 LYIMTALYFFILTLGRIYCGMHGILDITSGITIGIFCCAIRVFTNSYLDFDKFTIQSGVW 76
L + Y+ L GRIYCGMHGILD+ SG IGI C +R++ F I+ W
Sbjct: 189 LSCVVLFYYMTLVFGRIYCGMHGILDLVSGGLIGIVCFIVRMYFK--YRFPGLRIEEHWW 246
Query: 77 YPILSIITGAALLKFHARPIDECPCIEDSVAFIGVIMGIECSDWLSPRVYDSELYVMPFS 136
+P+ S+ G LL H +P+DECPC +DSVAF+GV+ GIEC DWL + +Y +
Sbjct: 247 FPLFSVGWGLLLLFKHVKPVDECPCFQDSVAFMGVVSGIECCDWLGKVFGVTLVYNLE-- 304
Query: 137 YSRIGITGTVLRIIVGILAVLTWK-TLSKKVIYWFLSKT--IGDDRPFTKEELKQ--HQE 191
G T+ R++VG+ V+ WK +SK +IY L K + DDR + + H+E
Sbjct: 305 -PNCGWRLTLARLLVGLPCVVIWKYVISKPMIYTLLIKVFHLKDDRNVAARKRLEATHKE 363
Query: 192 SA---EVRLFTPLANIDVIGRSIIYAGIPATVILICPLLYKLLDI 233
A E L+ ID++GR IIYAG+P TV++ P+L+ LL+I
Sbjct: 364 GASKYECPLYIGEPKIDILGRFIIYAGVPFTVVMCSPVLFSLLNI 408
>SPAC823.11 [I] KOG2822 Sphingoid base-phosphate phosphatase
Length = 411
Score = 99.4 bits (246), Expect = 4e-21
Identities = 51/153 (33%), Positives = 84/153 (54%), Gaps = 7/153 (4%)
Query: 16 YLYIMTALYFFILTLGRIYCGMHGILDITSGITIGIFCCAIRVFTNSYLDF-DKFTIQSG 74
+L + LY ++LGRIYCGMHG +D+++G +G+ + +F Y DF S
Sbjct: 176 FLLSLVLLYIASISLGRIYCGMHGFMDVSTGTILGV---TLAIFQWKYADFFHNVWSSSS 232
Query: 75 VWYPILSIITGAALLKFHARPIDECPCIEDSVAFIGVIMGIECSDWLSPRVYDSELYVMP 134
PILS++ + FH +P + C C+EDS++FI VIMGI+ W + S L+
Sbjct: 233 TSVPILSVVLALFFIWFHPQPAERCICLEDSISFISVIMGIDLGTWFASPESLSHLHDNL 292
Query: 135 FSYSRIGITGTVLRIIVGILAVLTWKTLSKKVI 167
SY + +R++ G+ +L WK+ +K+ +
Sbjct: 293 NSYFLLKF---FVRVLFGVCMILIWKSFAKQAL 322
>At3g58490 [I] KOG2822 Sphingoid base-phosphate phosphatase
Length = 416
Score = 60.1 bits (144), Expect = 3e-09
Identities = 48/158 (30%), Positives = 71/158 (44%), Gaps = 8/158 (5%)
Query: 16 YLYIMTALYFFILTLGRIYCGMHGILDITSGITIGIFCCAIRVFTNSYLDFDKFTIQSGV 75
Y + + L ++ GR+Y GMH ++DI SG+ IG+ + + N LD D T + V
Sbjct: 183 YGFALACLLVALIAFGRVYLGMHSVVDIVSGLAIGVLILGLWLTVNEKLD-DFITSKQNV 241
Query: 76 WYPILSIITGAALLKFHARPIDE--CPCIEDSVAFIGVIMGIECSDWLSPRVYDSELYVM 133
S T + L A P E P E AF GV +GI + + E
Sbjct: 242 ----SSFWTALSFLLLFAYPTPEHPTPSYEYHTAFNGVTLGIVTGVQQTYSQFHHEAAPR 297
Query: 134 PFSYSRIGITGTVLRIIVGILAVLTWKTLSKKVIYWFL 171
FS + I+ + R++VGI +L K SK + W L
Sbjct: 298 IFS-PELPISSYLGRVMVGIPTILLVKFCSKSLAKWTL 334
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.330 0.147 0.461
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,421,758
Number of Sequences: 60738
Number of extensions: 536277
Number of successful extensions: 1512
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1500
Number of HSP's gapped (non-prelim): 5
length of query: 235
length of database: 30,389,216
effective HSP length: 103
effective length of query: 132
effective length of database: 24,133,202
effective search space: 3185582664
effective search space used: 3185582664
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)