ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI1509 good I KOG2822 Lipid transport and metabolism Sphingoid base-phosphate phosphatase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI1509 516219 515584 -212
(212 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJL134w [I] KOG2822 Sphingoid base-phosphate phosphatase 201 5e-52
YKR053c [I] KOG2822 Sphingoid base-phosphate phosphatase 191 6e-49
SPAC823.11 [I] KOG2822 Sphingoid base-phosphate phosphatase 110 2e-24
At3g58490 [I] KOG2822 Sphingoid base-phosphate phosphatase 67 2e-11
Hs20537723 [I] KOG2822 Sphingoid base-phosphate phosphatase 58 8e-09
Hs13540569 [I] KOG2822 Sphingoid base-phosphate phosphatase 55 9e-08
>YJL134w [I] KOG2822 Sphingoid base-phosphate phosphatase
Length = 409
Score = 201 bits (512), Expect = 5e-52
Identities = 102/186 (54%), Positives = 129/186 (68%), Gaps = 5/186 (2%)
Query: 30 RQRVFSFSHLNVFDGKHPYSDPGNHDPDHFKPHMSKFRFECRQSLLKVIELTHTEAIILW 89
R+R + S+ N F + DPGNH DHF+ MSKFRF R+ LL V + + W
Sbjct: 11 RKRARTLSNPNDFQEPNYLLDPGNHPSDHFRTRMSKFRFNIREKLL-VFTNNQSFTLSRW 69
Query: 90 IQAHLRSKPMDVFFAFTAIMGSHTFYVVMLPIPRWTGFSVVTRDLVYILGYSIYLSGHFK 149
Q RS D++F +T++MGSHTFYV+ LP+P W G+ T+D+VYILGYSIYLSG FK
Sbjct: 70 -QKKYRSAFNDLYFTYTSLMGSHTFYVLCLPMPVWFGYFETTKDMVYILGYSIYLSGFFK 128
Query: 150 DYWCLPRPKSPPVHRITLSDYTTKEYGAPSSHTANATGVSLL---TVWYISQLSVSHEYL 206
DYWCLPRP++PP+HRITLS+YTTKEYGAPSSHTANATGVSLL +W + + SV + L
Sbjct: 129 DYWCLPRPRAPPLHRITLSEYTTKEYGAPSSHTANATGVSLLFLYNIWRMQESSVMVQLL 188
Query: 207 RIIFIL 212
+L
Sbjct: 189 LSCVVL 194
>YKR053c [I] KOG2822 Sphingoid base-phosphate phosphatase
Length = 404
Score = 191 bits (485), Expect = 6e-49
Identities = 89/153 (58%), Positives = 114/153 (74%), Gaps = 2/153 (1%)
Query: 44 GKHPYSDPGNHDPDHFKPHMSKFRFECRQSLLKVIELTHTEAIILWIQAHLRSKPMDVFF 103
GKH +DPGNH +HF+ MS RF+ RQ L + + + + +Q R+ DV+F
Sbjct: 26 GKHLLADPGNHPAEHFESQMSWLRFQTRQYLTRFTD--NQSDFVHSLQKKHRTPFRDVYF 83
Query: 104 AFTAIMGSHTFYVVMLPIPRWTGFSVVTRDLVYILGYSIYLSGHFKDYWCLPRPKSPPVH 163
+T++MGSH FYV++LP+P W G+ +TRD++Y+LGYSIYLSG+ KDYWCLPRPKSPPV
Sbjct: 84 KYTSLMGSHMFYVIVLPMPVWLGYRDLTRDMIYVLGYSIYLSGYLKDYWCLPRPKSPPVD 143
Query: 164 RITLSDYTTKEYGAPSSHTANATGVSLLTVWYI 196
RITLS+YTTKEYGAPSSH+ANAT VSLL W I
Sbjct: 144 RITLSEYTTKEYGAPSSHSANATAVSLLFFWRI 176
>SPAC823.11 [I] KOG2822 Sphingoid base-phosphate phosphatase
Length = 411
Score = 110 bits (274), Expect = 2e-24
Identities = 55/147 (37%), Positives = 88/147 (59%), Gaps = 4/147 (2%)
Query: 67 RFECRQSLLKVIELTHTEAIILW-IQAHLRSKPMDVFFAFTAIMGSHTFYVVMLPIPRWT 125
RF+ R+ +L ++ E +L+ IQ+ R+ +DV+F +TA +G+H F+++ LPI W+
Sbjct: 35 RFQLRELILPIVR---KETRLLYKIQSFFRNPWLDVYFMYTATLGTHVFFMLALPIFFWS 91
Query: 126 GFSVVTRDLVYILGYSIYLSGHFKDYWCLPRPKSPPVHRITLSDYTTKEYGAPSSHTANA 185
G T D+ + +Y SG KDY+CLPRP+SPP+ R+TLS EYG PS+HT NA
Sbjct: 92 GCIYYTLDITQLFAAGVYFSGCIKDYFCLPRPRSPPMVRLTLSSDAEYEYGFPSTHTTNA 151
Query: 186 TGVSLLTVWYISQLSVSHEYLRIIFIL 212
+++ + +S S + F+L
Sbjct: 152 MATGFYSLFLLLSMSDSMSSISYYFLL 178
>At3g58490 [I] KOG2822 Sphingoid base-phosphate phosphatase
Length = 416
Score = 66.6 bits (161), Expect = 2e-11
Identities = 35/101 (34%), Positives = 51/101 (49%), Gaps = 3/101 (2%)
Query: 99 MDVFFAFTAIMGSHTFYVVMLPIPRWTGFSVVTRDLVYILGYSIYLSGHFKDYWCLPRPK 158
+D FF+ + + S FY LP+ W+G + R + ++ + YL KD PRP
Sbjct: 69 LDAFFSGLSCVVSVPFYTAFLPLLFWSGHGRLARQMTLLIAFCDYLGNCIKDVVSAPRPS 128
Query: 159 SPPVHRITLS---DYTTKEYGAPSSHTANATGVSLLTVWYI 196
PPV RIT + + EYG PSSHT N +S + Y+
Sbjct: 129 CPPVRRITATKDEEDNAMEYGLPSSHTLNTVCLSGYLLHYV 169
>Hs20537723 [I] KOG2822 Sphingoid base-phosphate phosphatase
Length = 399
Score = 58.2 bits (139), Expect = 8e-09
Identities = 31/87 (35%), Positives = 43/87 (48%), Gaps = 2/87 (2%)
Query: 103 FAFTAIMGSHTFYVVMLPIPRWTGFSVVTRDLVYILGYSIYLSGHFKDYWCLPRPKSPPV 162
F F+A +G FY+ LP W ++R L+ I +Y+ KD PRP SPPV
Sbjct: 90 FQFSAALGQEVFYITFLPFTHWNIDPYLSRRLIIIWVLVMYIGQVAKDVLKWPRPSSPPV 149
Query: 163 HRITLSDYTTKEYGAPSSHTANATGVS 189
+ L EYG PS+H AT ++
Sbjct: 150 --VKLEKRLIAEYGMPSTHAMAATAIA 174
>Hs13540569 [I] KOG2822 Sphingoid base-phosphate phosphatase
Length = 441
Score = 54.7 bits (130), Expect = 9e-08
Identities = 33/102 (32%), Positives = 48/102 (46%), Gaps = 2/102 (1%)
Query: 92 AHLRSKPMDVFFAFTAIMGSHTFYVVMLPIPRWTGFSVVTRDLVYILGYSIYLSGHFKDY 151
A + + P+ F F +G+ FY++ P W +V R LV I +YL KD
Sbjct: 121 ARVSNWPLYCLFCFGTELGNELFYILFFPFWIWNLDPLVGRRLVVIWVLVMYLGQCTKDI 180
Query: 152 WCLPRPKSPPVHRITLSDYTTKEYGAPSSHTANATGVSLLTV 193
PRP SPPV + L + EY PS+H + T + + V
Sbjct: 181 IRWPRPASPPV--VKLEVFYNSEYSMPSTHAMSGTAIPISMV 220
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.324 0.137 0.430
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,357,923
Number of Sequences: 60738
Number of extensions: 559395
Number of successful extensions: 1517
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 1508
Number of HSP's gapped (non-prelim): 6
length of query: 212
length of database: 30,389,216
effective HSP length: 102
effective length of query: 110
effective length of database: 24,193,940
effective search space: 2661333400
effective search space used: 2661333400
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)