ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI1537 good J KOG2187 Translation, ribosomal structure and biogenesis tRNA uracil-5-methyltransferase and related tRNA-modifying enzymes
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI1537 529964 531697 578
(578 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKR056w [J] KOG2187 tRNA uracil-5-methyltransferase and related ... 597 e-170
SPAC4G8.07c [J] KOG2187 tRNA uracil-5-methyltransferase and rela... 367 e-101
At3g21300 [J] KOG2187 tRNA uracil-5-methyltransferase and relate... 125 1e-28
7293834 [J] KOG2187 tRNA uracil-5-methyltransferase and related ... 90 1e-17
HsM12232381 [J] KOG2187 tRNA uracil-5-methyltransferase and rela... 75 4e-13
At2g28450_2 [J] KOG2187 tRNA uracil-5-methyltransferase and rela... 70 7e-12
Hs21361612 [J] KOG2187 tRNA uracil-5-methyltransferase and relat... 66 1e-10
Hs20149688 [J] KOG2187 tRNA uracil-5-methyltransferase and relat... 62 3e-09
CE23823 [J] KOG2187 tRNA uracil-5-methyltransferase and related ... 57 1e-07
>YKR056w [J] KOG2187 tRNA uracil-5-methyltransferase and related
tRNA-modifying enzymes
Length = 617
Score = 597 bits (1540), Expect = e-170
Identities = 292/471 (61%), Positives = 375/471 (78%), Gaps = 7/471 (1%)
Query: 62 LDPTSALGVLQFEIDEILKEFSLARDAILNDVGCILNEER--DGPMHRLYHREVNSVEVL 119
++ TS +GVL+FE++++LK +L+R+ +LNDV ILN++ DGP+ YHREV +V+VL
Sbjct: 116 VETTSPMGVLEFEVNDLLKSQNLSREQVLNDVTSILNDKSSTDGPIVLQYHREVKNVKVL 175
Query: 120 KMGANGEGLALIPNPVDLDKKQIALIPFALPGDVVRIKTFKTHPHYVECDLLEVIKPSSK 179
++ +NG GLALI NPV+ +KKQ+ +IPF LPGDVV IK FKTHP+YVE DLL+V++ S
Sbjct: 176 EITSNGNGLALIDNPVETEKKQVVIIPFGLPGDVVNIKVFKTHPYYVESDLLDVVEKSPM 235
Query: 180 RNNDLIQCRYFGKCSGCQLQYLTYEDQLAFKKRTVMNAYKFFAPRLMEDKLVPEIGETMR 239
R +DLI+ +YFGK SG QL++LTY+DQL K++T+MNAYKFFAPRL+ +KL+P T+
Sbjct: 236 RRDDLIRDKYFGKSSGSQLEFLTYDDQLELKRKTIMNAYKFFAPRLVAEKLLPPFDTTVA 295
Query: 240 SPLEIGYRTKLTPHFDLPKR-MKGNGERPALGFGHKGRPQWRGNA--DGGSGPMLDIEEC 296
SPL+ GYRTK+TPHFD+PKR K RP LGFG KGRPQWR + GG G +LDI+EC
Sbjct: 296 SPLQFGYRTKITPHFDMPKRKQKELSVRPPLGFGQKGRPQWRKDTLDIGGHGSILDIDEC 355
Query: 297 IIGTKIINQGMTNERARFEREFTKYNKGATILLREHTKVIDPEIPVAEQL-DDGSEDIEG 355
++ T+++N+G+TNER +FE+EF Y KGATILLRE+T ++DP P EQL ++ S D G
Sbjct: 356 VLATEVLNKGLTNERRKFEQEFKNYKKGATILLRENTTILDPSKPTLEQLTEEASRDENG 415
Query: 356 KISYLQINDEENDKSLVKTCVTNTRQIVTEYINGYTFQFSAGEFFQNNNSILPSVTAYVR 415
ISY+++ D++N+ L KTCVTN RQIVTEY++GYTF FSAGEFFQNNNSILP VT YVR
Sbjct: 416 DISYVEVEDKKNNVRLAKTCVTNPRQIVTEYVDGYTFNFSAGEFFQNNNSILPIVTKYVR 475
Query: 416 KNLQIEGSKPED-PHYLVDAYCGSGLFSITSSKGVDRVIGVEVSADSVAFAERNAKANGV 474
NLQ ++ +LVDAYCGSGLFSI SSKGVD+VIGVE+SADSV+FAE+NAKANGV
Sbjct: 476 DNLQAPAKGDDNKTKFLVDAYCGSGLFSICSSKGVDKVIGVEISADSVSFAEKNAKANGV 535
Query: 475 ENCKFIVGKAEKIFGSIDTPCDRTSVILDPPRKGCDDIFMKQLAEYNPARI 525
ENC+FIVGKAEK+F SIDTP + TSVILDPPRKGCD++F+KQLA Y R+
Sbjct: 536 ENCRFIVGKAEKLFESIDTPSENTSVILDPPRKGCDELFLKQLAAYIQPRL 586
>SPAC4G8.07c [J] KOG2187 tRNA uracil-5-methyltransferase and related
tRNA-modifying enzymes
Length = 527
Score = 367 bits (943), Expect = e-101
Identities = 208/466 (44%), Positives = 278/466 (59%), Gaps = 61/466 (13%)
Query: 116 VEVLKMGANGEGLALIPNPVDLDKKQIALIPFALPGDVVRIKTFKTHPHYVECDLLEVIK 175
V++ + ++G+G+ L+ N + ++PF LPGD+V+ K Y D LEVI
Sbjct: 116 VKISYLSSSGDGIGLVCND-----QYAVVVPFTLPGDIVKAKLHFLADTYALADFLEVIS 170
Query: 176 PSSKRNNDLIQCRYFGKCSGCQLQYLTYEDQLAFKKRTVMNAYKFFAPRLMEDKLVPEIG 235
PS R++ LI+C YF KC GCQ Q L+Y+ QL KKR V A+++++ ++ L+P I
Sbjct: 171 PSEDRDDTLIKCPYFAKCGGCQYQMLSYDKQLLQKKRVVEKAFQYYSK--LDSSLLPAIK 228
Query: 236 ETMRSPLEIGYRTKLTPHFDLPKRMKGNGERPAL-GFGHKGRPQWRGNADGGSGPMLDIE 294
+T+ SPL+ YRTK+TPHFD+PK G + P + GF KGR + ++DIE
Sbjct: 229 DTVGSPLQYNYRTKITPHFDVPK---GGTKGPLIIGFQEKGRRR-----------VMDIE 274
Query: 295 ECIIGTKIINQGMTNERARFEREFTKYNKGATILLREHTKVIDPEIPVAEQLDDGSEDIE 354
EC I TK IN+ + + +GATIL+R D +ED
Sbjct: 275 ECPIATKTINEEYPKIIEDVQSRANTFKRGATILMR----------------DSATED-- 316
Query: 355 GKISYLQINDEENDKSLVKTCV-TNTRQIVTEYINGYTFQFSAGEFFQNNNSILPSVTAY 413
K CV T+ + IV E +F F AG FFQNNNSIL T+Y
Sbjct: 317 -----------------GKHCVITDHKMIVREQFGDLSFTFPAGAFFQNNNSILEKFTSY 359
Query: 414 VRKNLQIEGSKPED--PHYLVDAYCGSGLFSITSSKGVDRVIGVEVSADSVAFAERNAKA 471
VR+ L + E P Y VDAYCGSGLFS+ SKG VIGVE+SADSV +AE NAK
Sbjct: 360 VREQLLNPFGRKEHQRPKYFVDAYCGSGLFSVACSKGFLSVIGVEISADSVRYAEENAKR 419
Query: 472 NGVENCKFIVGKAEKIFGSIDTPCDRTSVILDPPRKGCDDIFMKQLAEYNPARIVYISCN 531
N V N FIVG+AEKIF SI+TP + T++++DPPRKGCD F+ QL EY+P RIVYISCN
Sbjct: 420 NNVSNATFIVGQAEKIFSSIETPPNETAMVIDPPRKGCDQSFLNQLLEYSPYRIVYISCN 479
Query: 532 VHSQARDVEYFLKETENGKHYKIESLRGFDFFPQTHHVESVCVLSR 577
VH+QARDV + L + E G+ YKI+ +RGFD FPQ+HHVES+ L++
Sbjct: 480 VHTQARDVGFLLSQ-EKGRSYKIDEIRGFDLFPQSHHVESILTLTK 524
>At3g21300 [J] KOG2187 tRNA uracil-5-methyltransferase and related
tRNA-modifying enzymes
Length = 598
Score = 125 bits (315), Expect = 1e-28
Identities = 126/511 (24%), Positives = 219/511 (42%), Gaps = 69/511 (13%)
Query: 109 YHREVNSVEVL--KMGANGEGLALIPNPVDLDKKQIALIPFALPGDVVRIKTFKTHPHYV 166
Y + +VE++ +G G+G+ + + + ALPG+ + + Y
Sbjct: 110 YPKRGETVELVCESLGFKGKGICKVDGT-----GYVVMCDRALPGERFLGRVTRRKGSYA 164
Query: 167 ECDLLEVIKPSSKRNNDLIQ--CRYFGKCSGCQLQYLTYEDQLAFKKRTVMNAYKFFAPR 224
E ++ I P + DL++ C Y C GC+ Q L+YE QL K+ V + R
Sbjct: 165 EVTKMKTISP----HKDLVEAPCEYASYCGGCKTQNLSYEAQLRAKEEQVHELIRHVG-R 219
Query: 225 LMEDKLVPEI--GETMRSPLEIGYRTKLTPHFD----LPKRM----KGNGERPALGFGHK 274
++ EI + ++ YR K+ F LP M + + ALG
Sbjct: 220 FSDNNPGLEIVLKPIVACDIQFHYRNKMEFSFGPQRWLPIEMLNERQDGPKNFALGL--- 276
Query: 275 GRPQWRGNADGGSGPMLDIEECIIGTKIINQGMTNERARF---EREFTKYNKGATILLRE 331
+A G +L++++C++ ++ N + + + E + Y+ + + +
Sbjct: 277 -------HAPGFFDKVLNVDKCLLQSEPGNLVLAAVQDCWRDPELSLSPYDCRSHVGFLK 329
Query: 332 H---------------------TKVIDPEIPVAEQLDDGSEDIEGKISYLQ-INDEENDK 369
H T PE+ + L D I +S + +N +
Sbjct: 330 HLMLRTGRNVETGSLELMVNFVTSSYKPEL--LKPLVDRISSIPQVVSIMNNVNSSVGNT 387
Query: 370 SL-VKTCVTNTRQIVTEYINGYTFQFSAGEFFQNNNSILPSVTAYVRKNLQIEGSKPEDP 428
S+ K + +TE + G TFQ SA FFQ N + + ++ ++G E
Sbjct: 388 SVGEKEYTLYGKDTITEVLRGLTFQISANSFFQTNTHQAEVLYKLIEESAGLKGDGSE-- 445
Query: 429 HYLVDAYCGSGLFSITSSKGVDRVIGVEVSADSVAFAERNAKANGVENCKFIVGKAEKIF 488
++D +CG+G +T ++ V G EV ++ A +NA+ NG+EN FI G KI
Sbjct: 446 -VVLDLFCGTGTIGLTLARRAKHVYGYEVVPQAITDAHKNAQINGIENATFIQGDLNKIG 504
Query: 489 GSIDTPCDRTSVIL-DPPRKGCDDIFMKQLAEYNPARIVYISCNVHSQARDVEYFLK--E 545
+ +++ DP R G +K L + RI+Y+SCN + ARD++Y E
Sbjct: 505 EDFGNNFPKPDIVISDPNRPGMHMKLIKFLLKLKSPRIIYVSCNPATCARDLDYLCHGVE 564
Query: 546 TENGKH-YKIESLRGFDFFPQTHHVESVCVL 575
+N K YK+ S++ D FP T H+E VC+L
Sbjct: 565 EKNLKGCYKLMSVQPVDMFPHTPHIECVCLL 595
>7293834 [J] KOG2187 tRNA uracil-5-methyltransferase and related
tRNA-modifying enzymes
Length = 624
Score = 89.7 bits (221), Expect = 1e-17
Identities = 67/212 (31%), Positives = 102/212 (47%), Gaps = 26/212 (12%)
Query: 383 VTEYINGYTFQFSAGEFFQNNNSILPSVTAYVRKNLQIEGSKPEDPHYLVDAYCGSGLFS 442
+T+ I G F+ S FFQ N A V I+ P ++D CG+G +
Sbjct: 376 ITDTIQGLQFRISPLAFFQINTE-----GANVLYQKAIDLVAPTKDTTMLDICCGTGTIT 430
Query: 443 ITSSKGVDRVIGVEVSADSVAFAERNAKANGVENCKFIVGKAEKIFGSI----------D 492
+ +K +V+GVE+ D++ AE NA+ANG++N KF G A+ S+
Sbjct: 431 LAFAKHCKKVMGVEIVPDAIKDAEFNAEANGIKNAKFFTGNADDFIKSMVREALYDQEPG 490
Query: 493 TPCDRTSVILDPPRKGCDDIFMKQLAEYNPA----RIVYISCNVHSQARD-VEYFLKETE 547
P D +V+ DPPR G + + +A A R+VY+SCN HS R+ +E E++
Sbjct: 491 KPLDLIAVV-DPPRAG---LHHRSIAAIRSADAINRLVYVSCNPHSAKRNFIELARPESK 546
Query: 548 --NGKHYKIESLRGFDFFPQTHHVESVCVLSR 577
G+ + +S D FP T H E V + R
Sbjct: 547 QYKGEPFYPKSAVAVDMFPHTMHTELVILFER 578
>HsM12232381 [J] KOG2187 tRNA uracil-5-methyltransferase and related
tRNA-modifying enzymes
Length = 475
Score = 74.7 bits (182), Expect = 4e-13
Identities = 54/201 (26%), Positives = 91/201 (44%), Gaps = 11/201 (5%)
Query: 383 VTEYINGYTFQFSAGEFFQNNNSILPSVTAYVRKNLQIEGSKPEDPHYLVDAYCGSGLFS 442
+ E + G TF+ S FFQ N + ++ Q++ ++D CG+G
Sbjct: 243 IHEDLLGLTFRISPHAFFQVNTPAAEVLYTVIQDWAQLDAGS-----MVLDVCCGTGTIG 297
Query: 443 ITSSKGVDRVIGVEVSADSVAFAERNAKANGVENCKFIVGKAEKIFGSIDT--PCDRTSV 500
+ ++ V RVIGVE+ ++V A NA+ N + N +F G+AE + ++ +
Sbjct: 298 LALARKVKRVIGVELCPEAVEDARVNAQDNELSNVEFHCGRAEDLVPTLVSRLASQHLVA 357
Query: 501 ILDPPRKGC-DDIFMKQLAEYNPARIVYISCNVHSQARDVEYFLKETEN---GKHYKIES 556
ILDPPR G + + N R++Y+SCN + + + N G ++
Sbjct: 358 ILDPPRAGLHSKVILAIRRAKNLRRLLYVSCNPRAAMGNFVDLCRAPSNRVKGIPFRPVK 417
Query: 557 LRGFDFFPQTHHVESVCVLSR 577
D FPQT H E + + R
Sbjct: 418 AVAVDLFPQTPHCEMLILFER 438
>At2g28450_2 [J] KOG2187 tRNA uracil-5-methyltransferase and related
tRNA-modifying enzymes
Length = 586
Score = 70.5 bits (171), Expect = 7e-12
Identities = 55/203 (27%), Positives = 89/203 (43%), Gaps = 31/203 (15%)
Query: 290 MLDIEECIIGTKIINQGMTNERARFEREFTKYNKGATILLREHTKVIDPEIPVAEQLDDG 349
ML ++ C++ K+ + FE + +GA + P +P+ +
Sbjct: 183 MLMVQVCVLNGKVATE--------FEELAKAFAEGA--------RASSPTLPLTVLVVQN 226
Query: 350 SEDIEG--------KISYLQINDEENDKSLVKTCVTNTRQIVTEYINGYTFQFSAGEFFQ 401
I ++ + I+D D+ +T R + ++IN F S FFQ
Sbjct: 227 HSGISNVAPPDAPLQVLAIPISDNGTDQEQTTNVLTEAR--IHDHINNLRFSISPTAFFQ 284
Query: 402 NNNSILPSVTAYVRKNLQIEGSKPEDPHYLVDAYCGSGLFSITSSKGVDRVIGVEVSADS 461
N +VTA ++ + + L D CG+G +T + V VIG+E++A +
Sbjct: 285 VN-----TVTAEKLYSIAGDWADLGPDTLLFDVCCGTGTIGLTLAHRVGMVIGIEMNASA 339
Query: 462 VAFAERNAKANGVENCKFIVGKA 484
VA AERNA NG+ NCKFI KA
Sbjct: 340 VADAERNATINGISNCKFICSKA 362
>Hs21361612 [J] KOG2187 tRNA uracil-5-methyltransferase and related
tRNA-modifying enzymes
Length = 562
Score = 66.2 bits (160), Expect = 1e-10
Identities = 44/152 (28%), Positives = 73/152 (47%), Gaps = 8/152 (5%)
Query: 383 VTEYINGYTFQFSAGEFFQNNNSILPSVTAYVRKNLQIEGSKPEDPHYLVDAYCGSGLFS 442
+ E + G TF+ S FFQ N + ++ Q++ ++D CG+G
Sbjct: 393 IHEDLLGLTFRISPHAFFQVNTPAAEVLYTVIQDWAQLDAGS-----MVLDVCCGTGTIG 447
Query: 443 ITSSKGVDRVIGVEVSADSVAFAERNAKANGVENCKFIVGKAEKIFGSI--DTPCDRTSV 500
+ ++ V RVIGVE+ ++V A NA+ N + N +F G+AE + ++
Sbjct: 448 LALARKVKRVIGVELCPEAVEDARVNAQDNELSNVEFHCGRAEDLVPTLVGRLASQHLVA 507
Query: 501 ILDPPRKGC-DDIFMKQLAEYNPARIVYISCN 531
ILDPPR G + + N R++Y+SCN
Sbjct: 508 ILDPPRAGLHSKVILAIRRAKNLRRLLYVSCN 539
>Hs20149688 [J] KOG2187 tRNA uracil-5-methyltransferase and related
tRNA-modifying enzymes
Length = 504
Score = 61.6 bits (148), Expect = 3e-09
Identities = 42/157 (26%), Positives = 80/157 (50%), Gaps = 10/157 (6%)
Query: 431 LVDAYCGSGLFSITSSKGVDRVIGVEVSADSVAFAERNAKANGVENCKFIVGKAEKIF-G 489
L+D CG+G+ ++ ++ RV+G+E+ +V A A NG+ N +F G+AEKI G
Sbjct: 348 LLDICCGTGVIGLSLAQHTSRVLGIELLEQAVEDARWTAAFNGITNSEFHTGQAEKILPG 407
Query: 490 SIDTPCDRTSV--ILDPPRKGCDDIFMKQLAEYNPAR-IVYISCNVHSQA-RDVEYFLKE 545
+ + D S+ +++P R G ++ + + +V++SC +H ++ R+V
Sbjct: 408 LLKSKEDGQSIVAVVNPARAGLHYKVIQAIRNFRAIHTLVFVSCKLHGESTRNVIELCCP 467
Query: 546 TEN-----GKHYKIESLRGFDFFPQTHHVESVCVLSR 577
+ G+ + ++ D FP T H E V + +R
Sbjct: 468 PDPAKKLLGEPFVLQQAVPVDLFPHTPHCELVLLFTR 504
>CE23823 [J] KOG2187 tRNA uracil-5-methyltransferase and related
tRNA-modifying enzymes
Length = 547
Score = 56.6 bits (135), Expect = 1e-07
Identities = 39/103 (37%), Positives = 53/103 (50%), Gaps = 15/103 (14%)
Query: 421 EGSKPEDPH-YLVDAYCGSGLFSITSSKGVDR-----VIGVEVSADSVAFAERNAKANGV 474
E PEDP L+D CG+G K + + IG+E+ A++V A++NAK N +
Sbjct: 428 EAPAPEDPGTILLDICCGTGTIGQCLLKNIQKSQKVCCIGIEMIAEAVEDAKQNAKQNKM 487
Query: 475 EN-CKFIVGKAEKIFGSI--------DTPCDRTSVILDPPRKG 508
E CK+I GKAE F S+ D R +LDPPR G
Sbjct: 488 ETCCKYIAGKAEDTFRSMRYHLPNGFDLRKSRVVGVLDPPRAG 530
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.139 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,017,084
Number of Sequences: 60738
Number of extensions: 1589715
Number of successful extensions: 3343
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 3313
Number of HSP's gapped (non-prelim): 16
length of query: 578
length of database: 30,389,216
effective HSP length: 112
effective length of query: 466
effective length of database: 23,586,560
effective search space: 10991336960
effective search space used: 10991336960
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)