ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI1570 good K KOG2351 Transcription RNA polymerase II, fourth largest subunit
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI1570 540920 540321 -200
(200 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJL140w [K] KOG2351 RNA polymerase II fourth largest subunit 253 2e-67
SPBC337.14 [K] KOG2351 RNA polymerase II fourth largest subunit 79 4e-15
CE28327_2 [K] KOG2351 RNA polymerase II fourth largest subunit 73 2e-13
Hs4758574 [K] KOG2351 RNA polymerase II fourth largest subunit 68 9e-12
At5g09920 [K] KOG2351 RNA polymerase II fourth largest subunit 57 1e-08
ECU08g0765 [K] KOG2351 RNA polymerase II fourth largest subunit 50 2e-06
>YJL140w [K] KOG2351 RNA polymerase II fourth largest subunit
Length = 221
Score = 253 bits (645), Expect = 2e-67
Identities = 143/223 (64%), Positives = 160/223 (71%), Gaps = 25/223 (11%)
Query: 1 MNTSTSTVQTKRRGLKKFVEEENAATLHLGEEFNLKQINHQGQEENLVALNLSESRLIIK 60
MN STST QT+RR LKK EEENAATL LG+EF LKQINHQG+EE L+ALNLSE+RL+IK
Sbjct: 1 MNVSTSTFQTRRRRLKKVEEEENAATLQLGQEFQLKQINHQGEEEELIALNLSEARLVIK 60
Query: 61 ETLQNRRKLFKTWASNGASNKXXXXXXXXN-----------------VN------VHTQT 97
E L RR+ FK S K N VN +H++T
Sbjct: 61 EALVERRRAFKR--SQKKHKKKHLKHENANDETTAVEDEDDDLDEDDVNADDDDFMHSET 118
Query: 98 QEKELQDIDRLLEQTTGGQNQALKQTMIYLTNFSRFRDQETVTAVSQLLSSTGLHPFEVA 157
+EKEL+ ID LLEQTTGG N+ LK TM YLTNFSRFRDQETV AV QLL STGLHPFEVA
Sbjct: 119 REKELESIDVLLEQTTGGNNKDLKNTMQYLTNFSRFRDQETVGAVIQLLKSTGLHPFEVA 178
Query: 158 QLGSLSCEDADEAKTLIPSLGTKISDEDLERILKELSNLETLY 200
QLGSL+C+ ADEAKTLIPSL KISD++LERILKELSNLETLY
Sbjct: 179 QLGSLACDTADEAKTLIPSLNNKISDDELERILKELSNLETLY 221
>SPBC337.14 [K] KOG2351 RNA polymerase II fourth largest subunit
Length = 135
Score = 79.0 bits (193), Expect = 4e-15
Identities = 60/182 (32%), Positives = 88/182 (47%), Gaps = 52/182 (28%)
Query: 15 LKKFVEEENAATLHLGEEFNLKQINHQGQEENLVALNLSESRLIIKETLQNRRKLFKTWA 74
+ + + EE+AA L LG EF EN L +SE++++I+ L R +
Sbjct: 1 MPRAIFEEDAAQLKLGPEF-----------ENEDMLTVSEAKILIETVLAQRAR-----E 44
Query: 75 SNGASNKXXXXXXXXNVNVHTQTQEKELQDIDRLLEQTTGGQNQALKQTMIYLTNFSRFR 134
+NG E + D+ +K+T+ Y F+RF+
Sbjct: 45 TNG---------------------EIPMTDV--------------MKKTVAYFNVFARFK 69
Query: 135 DQETVTAVSQLLSSTGLHPFEVAQLGSLSCEDADEAKTLIPSLGTKISDEDLERILKELS 194
E A ++L + H FE AQLG+L CEDA+EA+TLIPSL KI D++L+ IL ELS
Sbjct: 70 TAEATYACERILGNR-FHKFERAQLGTLCCEDAEEARTLIPSLANKIDDQNLQGILDELS 128
Query: 195 NL 196
L
Sbjct: 129 TL 130
>CE28327_2 [K] KOG2351 RNA polymerase II fourth largest subunit
Length = 133
Score = 73.2 bits (178), Expect = 2e-13
Identities = 35/77 (45%), Positives = 52/77 (67%)
Query: 122 QTMIYLTNFSRFRDQETVTAVSQLLSSTGLHPFEVAQLGSLSCEDADEAKTLIPSLGTKI 181
+T+ Y SRF+++ET+ AV + S LH FEVAQ+ +L E+A+EAK L+PSL KI
Sbjct: 55 KTLNYARRMSRFKNRETIRAVRAIFSEKHLHKFEVAQIANLCPENAEEAKALVPSLENKI 114
Query: 182 SDEDLERILKELSNLET 198
+ +LE +LK+L + T
Sbjct: 115 EESELEEVLKDLQSKRT 131
>Hs4758574 [K] KOG2351 RNA polymerase II fourth largest subunit
Length = 142
Score = 67.8 bits (164), Expect = 9e-12
Identities = 38/104 (36%), Positives = 61/104 (58%), Gaps = 3/104 (2%)
Query: 90 NVNVHTQTQEKELQDIDRLLEQTTGGQNQALKQTMIYLTNFSRFRDQETVTAVSQLLSST 149
N VH + ++ Q+ EQ ++ +T+ Y FSRF+++ET+ +V LL
Sbjct: 34 NSEVHMLLEHRKQQNESAEDEQEL---SEVFMKTLNYTARFSRFKNRETIASVRSLLLQK 90
Query: 150 GLHPFEVAQLGSLSCEDADEAKTLIPSLGTKISDEDLERILKEL 193
LH FE+A L +L E A+E+K LIPSL + DE+L++IL ++
Sbjct: 91 KLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDI 134
>At5g09920 [K] KOG2351 RNA polymerase II fourth largest subunit
Length = 138
Score = 57.4 bits (137), Expect = 1e-08
Identities = 31/99 (31%), Positives = 57/99 (57%), Gaps = 3/99 (3%)
Query: 99 EKELQDIDRLLEQTTGGQNQALKQTMIYLTNFSRFRDQETVTAVSQLLSSTGLHPFEVAQ 158
E + + + ++ E +Q ++++ Y+ FSR+++ + V V ++LS L FE+
Sbjct: 34 EHKFEQLQQISEDPMNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRHQLTEFELCV 93
Query: 159 LGSLSCEDADEAKTLIPSLGTK---ISDEDLERILKELS 194
LG+L E +EA ++PSL TK DE +E++L +LS
Sbjct: 94 LGNLCPETVEEAVAMVPSLKTKGRAHDDEAIEKMLNDLS 132
>ECU08g0765 [K] KOG2351 RNA polymerase II fourth largest subunit
Length = 104
Score = 50.4 bits (119), Expect = 2e-06
Identities = 25/80 (31%), Positives = 46/80 (57%), Gaps = 1/80 (1%)
Query: 117 NQALKQTMIYLTNFSRFRDQETVTAVSQLLSSTGLHPFEVAQLGSLSCEDADEAKTLIPS 176
++ + T+ YL +F R +D+ + LS G E+A GSL + +EAK+L+PS
Sbjct: 25 SKVFRSTLGYLDDFCRIKDKSVAEDLRTTLSGLGFGEVEIALFGSLFPQSVEEAKSLVPS 84
Query: 177 LGTKISDEDLERILKELSNL 196
LGT+ D+ + + ++++ L
Sbjct: 85 LGTR-DDDAINQAVEKIQQL 103
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.310 0.126 0.332
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,083,109
Number of Sequences: 60738
Number of extensions: 366829
Number of successful extensions: 1037
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1027
Number of HSP's gapped (non-prelim): 8
length of query: 200
length of database: 30,389,216
effective HSP length: 101
effective length of query: 99
effective length of database: 24,254,678
effective search space: 2401213122
effective search space used: 2401213122
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)