ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI1594 good H KOG2900 Coenzyme transport and metabolism Biotin synthase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI1594 545949 547085 379
(379 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGR286c [H] KOG2900 Biotin synthase 576 e-164
SPCC320.01c [H] KOG2900 Biotin synthase 389 e-108
At2g43360 [H] KOG2900 Biotin synthase 350 2e-96
>YGR286c [H] KOG2900 Biotin synthase
Length = 375
Score = 576 bits (1484), Expect = e-164
Identities = 275/349 (78%), Positives = 308/349 (87%), Gaps = 3/349 (0%)
Query: 19 ALKESVAGENVS---AISYALSLNEPQHVWTKEQLAEIYHTPLMELMHQAQLQHRKWHDP 75
A+K + A S A+ YALSL+EP H WTK QL EIYHTPL+EL H AQLQHRKWHDP
Sbjct: 24 AVKSTTASSEASTLGALQYALSLDEPSHSWTKSQLKEIYHTPLLELTHAAQLQHRKWHDP 83
Query: 76 TKVQLCTLMNIKAGGCSEDCKYCAQSSRYDTGLKAEKMISVEEVIKEAQIAKENGSTRFC 135
TKVQLCTLMNIK+GGCSEDCKYCAQSSR DTGLKAEKM+ V+EVIKEA+ AK NGSTRFC
Sbjct: 84 TKVQLCTLMNIKSGGCSEDCKYCAQSSRNDTGLKAEKMVKVDEVIKEAEEAKRNGSTRFC 143
Query: 136 LGAAWREMRGRKSALKRIGEMIGKINEMGMESCVTLGMVDETQAKQLKDAGLTAYNHNID 195
LGAAWR+M+GRKSA+KRI EM+ K+N+MG+E+CVTLGMVD+ QAKQLKDAGLTAYNHNID
Sbjct: 144 LGAAWRDMKGRKSAMKRIQEMVTKVNDMGLETCVTLGMVDQDQAKQLKDAGLTAYNHNID 203
Query: 196 TSREHYPKVISTRSYDDRLQTIKNVQDAGIKACTGGILGLGETEDDHIGFVHTLANMSPH 255
TSREHY KVI+TR+YDDRLQTIKNVQ++GIKACTGGILGLGE+EDDHIGF++TL+NMSPH
Sbjct: 204 TSREHYSKVITTRTYDDRLQTIKNVQESGIKACTGGILGLGESEDDHIGFIYTLSNMSPH 263
Query: 256 PESLPINRLVSIKGTPMHXXXXXXXXXXXXFDEILRTVALARIVMPQAIIRLAAGRYTMK 315
PESLPINRLV+IKGTPM FDEILRT+A ARIVMP+AIIRLAAGRYTMK
Sbjct: 264 PESLPINRLVAIKGTPMAEELADPKSKKLQFDEILRTIATARIVMPKAIIRLAAGRYTMK 323
Query: 316 ETEQFLCFMAGCNAIFVGKKMLTTMCTGWDEDKAMLSKWGLQPMESFSY 364
ETEQF+CFMAGCN+IF GKKMLTTMC GWDEDKAML+KWGLQPME+F Y
Sbjct: 324 ETEQFVCFMAGCNSIFTGKKMLTTMCNGWDEDKAMLAKWGLQPMEAFKY 372
>SPCC320.01c [H] KOG2900 Biotin synthase
Length = 363
Score = 389 bits (1000), Expect = e-108
Identities = 187/335 (55%), Positives = 246/335 (72%), Gaps = 6/335 (1%)
Query: 33 SYALSLNEPQHVWTKEQLAEIYHTPLMELMHQAQLQHRKWHDPTKVQLCTLMNIKAGGCS 92
S ALSL ++ WT+E++ +IY TPL++L+ +A HRK+HDP KVQ CTL++IK GGC+
Sbjct: 13 SSALSL--VRNNWTREEIQKIYDTPLIDLIFRAASIHRKFHDPKKVQQCTLLSIKTGGCT 70
Query: 93 EDCKYCAQSSRYDTGLKAEKMISVEEVIKEAQIAKENGSTRFCLGAAWREMRGRKSALKR 152
EDCKYCAQSSRY+TG+KA K++ ++EV+++A+IAK GSTRFC+G+AWR++ GR K
Sbjct: 71 EDCKYCAQSSRYNTGVKATKLMKIDEVLEKAKIAKAKGSTRFCMGSAWRDLNGRNRTFKN 130
Query: 153 IGEMIGKINEMGMESCVTLGMVDETQAKQLKDAGLTAYNHNIDTSREHYPKVISTRSYDD 212
I E+I ++ M ME CVTLGM++E QAK+LKDAGLTAYNHN+DTSRE+Y K+ISTR+YD+
Sbjct: 131 ILEIIKEVRSMDMEVCVTLGMLNEQQAKELKDAGLTAYNHNLDTSREYYSKIISTRTYDE 190
Query: 213 RLQTIKNVQDAGIKACTGGILGLGETEDDHIGFVHTLANMSPHPESLPINRLVSIKGTPM 272
RL TI N++ AG+K C+GGILGLGE + D +G +H+LA M HPES+P N LV I GTP+
Sbjct: 191 RLNTIDNLRKAGLKVCSGGILGLGEKKHDRVGLIHSLATMPTHPESVPFNLLVPIPGTPV 250
Query: 273 HXXXXXXXXXXXXFDEILRTVALARIVMPQAIIRLAAGRYTMKETEQFLCFMAGCNAIFV 332
LR++A ARI MP+ IIR AAGR T E+EQ L FMAG NA+F
Sbjct: 251 ----GDAVKERLPIHPFLRSIATARICMPKTIIRFAAGRNTCSESEQALAFMAGANAVFT 306
Query: 333 GKKMLTTMCTGWDEDKAMLSKWGLQPMESFSYSAN 367
G+KMLTT WD D + WGL+ M+SF Y +
Sbjct: 307 GEKMLTTPAVSWDSDSQLFYNWGLEGMQSFEYGTS 341
>At2g43360 [H] KOG2900 Biotin synthase
Length = 378
Score = 350 bits (898), Expect = 2e-96
Identities = 170/332 (51%), Positives = 237/332 (71%), Gaps = 7/332 (2%)
Query: 41 PQHVWTKEQLAEIYHTPLMELMHQAQLQHRKWHDPTKVQLCTLMNIKAGGCSEDCKYCAQ 100
P++ W+++++ +Y +PL++L+ HR H+ +VQ CTL++IK GGCSEDC YC Q
Sbjct: 44 PRNDWSRDEIKSVYDSPLLDLLFHGAQVHRHVHNFREVQQCTLLSIKTGGCSEDCSYCPQ 103
Query: 101 SSRYDTGLKAEKMISVEEVIKEAQIAKENGSTRFCLGAAWREMRGRKSALKRIGEMIGKI 160
SSRY TG+KA++++S + VI A+ AKE GSTRFC+GAAWR+ GRK+ +I E I +I
Sbjct: 104 SSRYSTGVKAQRLMSKDAVIDAAKKAKEAGSTRFCMGAAWRDTIGRKTNFSQILEYIKEI 163
Query: 161 NEMGMESCVTLGMVDETQAKQLKDAGLTAYNHNIDTSREHYPKVISTRSYDDRLQTIKNV 220
MGME C TLGM+++ QA +LK AGLTAYNHN+DTSRE+YP VI+TRSYDDRL+T+ +V
Sbjct: 164 RGMGMEVCCTLGMIEKQQALELKKAGLTAYNHNLDTSREYYPNVITTRSYDDRLETLSHV 223
Query: 221 QDAGIKACTGGILGLGETEDDHIGFVHTLANMSPHPESLPINRLVSIKGTPMHXXXXXXX 280
+DAGI C+GGI+GLGE E+D IG +HTLA + HPES+PIN L+++KGTP+
Sbjct: 224 RDAGINVCSGGIIGLGEAEEDRIGLLHTLATLPSHPESVPINALLAVKGTPLEDQKPVEI 283
Query: 281 XXXXXFDEILRTVALARIVMPQAIIRLAAGRYTMKETEQFLCFMAGCNAIFVGKKMLTTM 340
E++R + ARIVMP+A++RL+AGR +EQ LCF+AG N+IF G+K+LTT
Sbjct: 284 W------EMIRMIGTARIVMPKAMVRLSAGRVRFSMSEQALCFLAGANSIFTGEKLLTTP 337
Query: 341 CTGWDEDKAMLSKWGLQPMESFSYSANKAPAE 372
+D D+ M GL P S+S + + +E
Sbjct: 338 NNDFDADQLMFKTLGLIPKPP-SFSEDDSESE 368
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.132 0.390
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,709,479
Number of Sequences: 60738
Number of extensions: 806798
Number of successful extensions: 2098
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2093
Number of HSP's gapped (non-prelim): 3
length of query: 379
length of database: 30,389,216
effective HSP length: 108
effective length of query: 271
effective length of database: 23,829,512
effective search space: 6457797752
effective search space used: 6457797752
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)