ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI1609 good K KOG3095 Transcription Transcription initiation factor IIE, beta subunit
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI1609 552438 553295 286
(286 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKR062w [K] KOG3095 Transcription initiation factor IIE beta sub... 304 8e-83
SPCC1672.08c [K] KOG3095 Transcription initiation factor IIE bet... 160 1e-39
ECU06g0350 [K] KOG3095 Transcription initiation factor IIE beta ... 67 4e-11
At4g20330 [K] KOG3095 Transcription initiation factor IIE beta s... 64 3e-10
At4g21010 [K] KOG3095 Transcription initiation factor IIE beta s... 53 4e-07
Hs4504195 [K] KOG3095 Transcription initiation factor IIE beta s... 50 3e-06
>YKR062w [K] KOG3095 Transcription initiation factor IIE beta subunit
Length = 328
Score = 304 bits (779), Expect = 8e-83
Identities = 170/322 (52%), Positives = 209/322 (64%), Gaps = 41/322 (12%)
Query: 5 LLANLNAFKNKVKTSPALTTRKVLANKDRD------GQTN-------------------- 38
LLANLNAFK+KVK++P + KV K D G T
Sbjct: 8 LLANLNAFKSKVKSAPVIAPAKVGQKKTNDTVITIDGNTRKRTASERAQENTLNSAKNPV 67
Query: 39 -------GNGKSTEVIEVDDPNSDEK--DMPLKKRAKSSSVAPTGQLDST-----HLSTK 84
S+ I +DD + DE P KK S A Q + T H S+K
Sbjct: 68 LVDIKKEAGSNSSNAISLDDDDDDEDFGSSPSKKVRPGSIAAAALQANQTDISKSHDSSK 127
Query: 85 MLIATEYIQERGEAVPVDQIESYLSLPKDNNVIPMLKGLQKFKFDPKRNTLQYVSIYDVH 144
+L ATEYIQ++G+ V V+++ YLS+ KD+ VI +LK L + +FDPK+ T +Y+S YDVH
Sbjct: 128 LLWATEYIQKKGKPVLVNELLDYLSMKKDDKVIELLKKLDRIEFDPKKGTFKYLSTYDVH 187
Query: 145 SAEELLQLLRSQATFKGISCKELKDGWPQCFDTIDELEAKNRILVLRTKKDNSPRFVWYN 204
S ELL+LLRSQ TFKGISCK+LKDGWPQC +TI++LE ++ILVLRTKKD +PR+VWYN
Sbjct: 188 SPSELLKLLRSQVTFKGISCKDLKDGWPQCDETINQLEEDSKILVLRTKKDKTPRYVWYN 247
Query: 205 HAGPSRQIDEEFVTMWEACKLPQRSELPRKLQDLGLKPASVDPATIKNEQTTRKEVKKRR 264
G + IDEEFV MWE +LPQ +ELPRKLQDLGLKPASVDPATIK QT R EVKK+R
Sbjct: 248 SGGNLKCIDEEFVKMWENVQLPQFAELPRKLQDLGLKPASVDPATIK-RQTKRVEVKKKR 306
Query: 265 SRKGKVTNTHMAGILRDYSDRV 286
RKGK+TNTHM GIL+DYS RV
Sbjct: 307 QRKGKITNTHMTGILKDYSHRV 328
>SPCC1672.08c [K] KOG3095 Transcription initiation factor IIE beta subunit
Length = 285
Score = 160 bits (406), Expect = 1e-39
Identities = 100/291 (34%), Positives = 155/291 (52%), Gaps = 21/291 (7%)
Query: 3 NDLLANLNAFKNKVKTSPALTTRKVLANKDRDGQTNGNGKSTEVIEVDDPNSDEKDMP-- 60
+ L L++FK KV P K + Q + T + +D +S P
Sbjct: 2 SSLSDQLSSFKKKVANQPIYA-------KPQPRQP-ASPTPTAYLNSNDGHSSAASSPGS 53
Query: 61 --LKKRAKSSSVAPTGQLDS---THLSTKMLIATEYIQERGEAVPVDQIESYLSLPKDNN 115
LKK+ +S+ + DS TH +++ A EY++ER E ++I SYLS P
Sbjct: 54 YSLKKKRSKTSLVYSQPADSGVGTHYLSQLHYAVEYLKERNEPKTAEEIASYLSTPLTPM 113
Query: 116 VIPMLKGLQKFKFDPKRNTLQYVSIYDVHSAEELLQLLRSQATFKGISCKELKDGWPQCF 175
++ +LK + +D + T + ++++ S LL L SQ T G+S KEL+DGWP
Sbjct: 114 LLNLLKKNNRIYYDERNETFTFKPLHNIRSGAGLLAYLDSQKTHVGMSIKELRDGWPNVT 173
Query: 176 DTIDELEAKNRILVLRTKKDNSPRFVWYNHAGPSRQIDEEFVTMWEACKLPQRSELPRKL 235
++ELE + +L+LRT+KD P+ VW N +D+EF +W +P +L +L
Sbjct: 174 VELEELEKQGEVLLLRTRKDGVPKMVWRNDKSCDCHVDKEFQQVWHEIPIPPTLDLASEL 233
Query: 236 QDLGLKPASVDPATIK---NEQTTRKEVKKRRSRKGKVTNTHMAGILRDYS 283
GLKP SVDP+T+K + QT ++ KK ++R+GK+TNTH+ ILRDYS
Sbjct: 234 GKYGLKPTSVDPSTVKRAGHNQTPKQ--KKPKTRRGKITNTHL-NILRDYS 281
>ECU06g0350 [K] KOG3095 Transcription initiation factor IIE beta subunit
Length = 247
Score = 66.6 bits (161), Expect = 4e-11
Identities = 45/203 (22%), Positives = 95/203 (46%), Gaps = 13/203 (6%)
Query: 80 HLSTKMLIATEYIQERGEAVPVDQIESYLSLPKDNNVIPMLKGLQKFKFDPK----RNTL 135
H +T + +++ + ++I+ + NN + LQ K +P+ +TL
Sbjct: 45 HTNTYIHAVISLLKQHDRPLSFEEIKDKTRIDLHNNYVL----LQSIKRNPRIVATHSTL 100
Query: 136 QYVSIYDVHSAEELLQLLRSQATFKGISCKELKDGWPQCFDTIDELEAKNRILVLRTKKD 195
+ +Y + S E++ +++R +G+ +L D ++EL+ N I++L
Sbjct: 101 MFKPLYSIRSVEDMRKVVRGLGGEEGLEMSKLMDSPVNVVPFVEELKKSNEIIILNDI-- 158
Query: 196 NSPRFVWYNHAGPSRQIDEEFVTMWEACKLPQRSELPRKLQDLGLKPASVDPATIKNEQT 255
+ V++N S +D + ++W ++ +L R+L GLK V+ +K T
Sbjct: 159 DGSEVVFWNDTQES-PVDPQIKSLWNQVRITTYHDLIRELNTAGLKTEKVE--NVKKRPT 215
Query: 256 TRKEVKKRRSRKGKVTNTHMAGI 278
+ + +R RK ++TNTH+ G+
Sbjct: 216 IKIKKDRRSRRKIRITNTHVKGL 238
>At4g20330 [K] KOG3095 Transcription initiation factor IIE beta subunit
Length = 286
Score = 63.5 bits (153), Expect = 3e-10
Identities = 57/279 (20%), Positives = 121/279 (42%), Gaps = 22/279 (7%)
Query: 14 NKVKTSPALTTRKVLANKDRDGQTNGNGKSTEVIEVDDPNSDEKDMPLKKRAKSSSVAPT 73
NK + T + ++++R + N I P++ P+K + + +
Sbjct: 11 NKQQVKCQSTLSSIASSRERTSSSRQNVPLPAAITQKKPDA----APVKFSSDTERLQNI 66
Query: 74 GQLDSTHLSTKMLIATEYIQERGEAVPVDQIESYLSLPKDNN--VIPMLKGLQKFKFDPK 131
+ + ++ + + ER A+ +QI + + N V L+ K +D +
Sbjct: 67 NNIRKAPVGAQIKRVIDLLYERRLALTPEQINEWCHVDMHANKAVFDSLRKNPKAHYDGR 126
Query: 132 RNTLQYVSIYDVHSAEELLQLLRSQATFKGISCKELKDGWPQCFDTIDELEAKNRILVLR 191
R Y + +DV+ +LL L+R GI+ +LKD +P + + L A I +L
Sbjct: 127 R--FSYKATHDVNDKNQLLSLVRKY--LDGIAVVDLKDAYPNVMEDLKALSASGDIYLLS 182
Query: 192 TKKDNSPRFVWYNHAGPSRQIDEEFVTMWEACKLP-QRSELPRKLQDLGLKP-------- 242
+++ + N ++D+EF ++ +P ++ ++L +GLKP
Sbjct: 183 NSQED---IAYPNDFKCEIKVDDEFKALFRDINIPNDMLDVEKELLKIGLKPATNTAERR 239
Query: 243 ASVDPATIKNEQTTRKEVKKRRSRKGKVTNTHMAGILRD 281
A+ I N+ +K+ K+ S++ K+TN H+ + ++
Sbjct: 240 AAAQTHGISNKPKDKKKKKQEISKRTKLTNAHLPELFQN 278
>At4g21010 [K] KOG3095 Transcription initiation factor IIE beta subunit
Length = 275
Score = 53.1 bits (126), Expect = 4e-07
Identities = 45/205 (21%), Positives = 91/205 (43%), Gaps = 15/205 (7%)
Query: 81 LSTKMLIATEYIQERGEAVPVDQIES--YLSLPKDNNVIPMLKGLQKFKFDPKRNTLQYV 138
+ +M I + + + A +QI Y+ + + V L+ K +D +R Y
Sbjct: 70 VGAQMKIVIDLLFKTRLAYTAEQINEACYVDMHNNKAVFDSLRKNPKVHYDGRR--FSYK 127
Query: 139 SIYDVHSAEELLQLLRSQATFKGISCKELKDGWPQCFDTIDELEAKNRILVLRTKKDNSP 198
+ +++ ++LL + K I +LKD +P + + L++ I L + D+
Sbjct: 128 ATHNIKDKKQLLSFVNKSD--KVIDVSDLKDAYPNVMEDLKSLKSSGEIFWLLSNTDSKE 185
Query: 199 RFVWYNHAGPSRQIDEEFVTMWEACKLPQRSELPRKLQDLGLKPASVDPAT--------I 250
V+ N+ + ID+E ++ E+ ++L +GLKPA+ +
Sbjct: 186 GTVYRNNMEYPK-IDDELKALFRDIIPSDMLEVEKELLKIGLKPATNIAERRAAEQLHGV 244
Query: 251 KNEQTTRKEVKKRRSRKGKVTNTHM 275
N+ +K+ KK + + K+TN+HM
Sbjct: 245 SNKPKDKKKKKKEITNRTKLTNSHM 269
>Hs4504195 [K] KOG3095 Transcription initiation factor IIE beta subunit
Length = 291
Score = 50.4 bits (119), Expect = 3e-06
Identities = 41/176 (23%), Positives = 76/176 (42%), Gaps = 19/176 (10%)
Query: 113 DNNVIPMLKGLQKFKFDPKRNTLQYVSIYDVHSAEELLQLL--RSQATFKGISCKELKDG 170
+N I ++ G K+ F PK Y+V + LL+LL Q GI +++++
Sbjct: 126 NNPKIEVIDG--KYAFKPK---------YNVRDKKALLRLLDQHDQRGLGGILLEDIEEA 174
Query: 171 WPQCFDTIDELEAKNRILVLRTKKDNSPRFVWYNHAGPSRQIDEEFVTMWEACKLPQRSE 230
P + L + +L + + + +++N +DEEF +W + + E
Sbjct: 175 LPNSQKAVKALGDQ----ILFVNRPDKKKILFFNDKSCQFSVDEEFQKLWRSVTVDSMDE 230
Query: 231 --LPRKLQDLGLKPASVDPATIKNEQTTRKEVKKRRSRKGKVTNTHMAGILRDYSD 284
+ L+ G+ RK+ ++ R+ K N H+AG+L+DYSD
Sbjct: 231 EKIEEYLKRQGISSMQESGPKKVAPIQRRKKPASQKKRRFKTHNEHLAGVLKDYSD 286
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.313 0.130 0.368
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,648,125
Number of Sequences: 60738
Number of extensions: 788362
Number of successful extensions: 2046
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 2037
Number of HSP's gapped (non-prelim): 6
length of query: 286
length of database: 30,389,216
effective HSP length: 105
effective length of query: 181
effective length of database: 24,011,726
effective search space: 4346122406
effective search space used: 4346122406
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)