ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI1658 good GM KOG4748 Carbohydrate transport and metabolism Subunit of Golgi mannosyltransferase complex
r_klactI1658 good GM KOG4748 Cell wall/membrane/envelope biogenesis Subunit of Golgi mannosyltransferase complex
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI1658 568689 569924 412
(412 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJL183w [GM] KOG4748 Subunit of Golgi mannosyltransferase complex 396 e-110
SPBC8D2.17 [GM] KOG4748 Subunit of Golgi mannosyltransferase com... 92 1e-18
SPAC22E12.06c [GM] KOG4748 Subunit of Golgi mannosyltransferase ... 90 5e-18
SPBC1289.13c [GM] KOG4748 Subunit of Golgi mannosyltransferase c... 87 6e-17
SPCC736.04c [GM] KOG4748 Subunit of Golgi mannosyltransferase co... 74 3e-13
SPAC5H10.13c [GM] KOG4748 Subunit of Golgi mannosyltransferase c... 74 3e-13
SPAC5H10.11 [GM] KOG4748 Subunit of Golgi mannosyltransferase co... 70 6e-12
YDR245w [GM] KOG4748 Subunit of Golgi mannosyltransferase complex 68 3e-11
SPAC637.06 [GM] KOG4748 Subunit of Golgi mannosyltransferase com... 66 8e-11
>YJL183w [GM] KOG4748 Subunit of Golgi mannosyltransferase complex
Length = 422
Score = 396 bits (1017), Expect = e-110
Identities = 197/423 (46%), Positives = 280/423 (65%), Gaps = 14/423 (3%)
Query: 1 MPFIPKNKKKWTKSFKV---WISELKFYLHKRNKFSMLLLISVIIFLLYELTSSL-PSKS 56
M P+ K K S V W + L F +K L +I+ +F+LY ++ P
Sbjct: 1 MAIKPRTKGKTYSSRSVGSQWFNRLGFKQNKYGTCKFLSIITAFVFILYFFSNRFYPISR 60
Query: 57 SAGYRIPKTHGMYQNEVQSSTPLIFPSVQDHGTLKELGVEGLFTLRIDVDGNREYVFKPE 116
SAG +HG+Y NE+ +S+ LI+P V+ LK++ V GL+ R++VDG++ + KPE
Sbjct: 61 SAGASYSPSHGLYINEIPASSRLIYPHVEHVPVLKQMTVRGLYITRLEVDGSKRLILKPE 120
Query: 117 DTPMTEAERKELAETDKKKFVKRTFLDHGKLVYKNKANHPEVVIVTIIDFENYSLDHIIN 176
+ +T+ E+K+ TD+ VK +FLDHGKLVY+ + PEVV+VT+IDFENY L+ II
Sbjct: 121 ENALTDEEKKKT--TDQILLVKHSFLDHGKLVYRKSNDAPEVVVVTLIDFENYELETIIQ 178
Query: 177 TVQNRVDYAQRHNYGTYVRWAQEFLPQVQKQSMEASYEFIKPLAVRAAMNAFPYAKYFWF 236
VQNRVDYAQ+H YG Y+RW QEFLP ++ Q++ SYEFIKPL +RAAM+AFP AKY F
Sbjct: 179 IVQNRVDYAQKHQYGVYIRWIQEFLPVLENQNLAESYEFIKPLVIRAAMHAFPTAKYIHF 238
Query: 237 FDQNGLIMQLDTSLQQQLLEPKVLDAAVLKSVPVFEKSNIKTYKHFDAKTAKIIIPKTGA 296
DQ+ L+M LD SLQ+ LL+PK++D A+LK+VPV SNIKTY HF+ +AKIIIP
Sbjct: 239 VDQDALLMNLDLSLQKYLLDPKIMDLALLKNVPVVANSNIKTYNHFEYSSAKIIIPHDAG 298
Query: 297 GKLDTSSFVVTPSIHGKAFLDYINDPLVRNYPWPSS---FTSAIGHLLQWHPRILKRTAL 353
G +D SSFV+ +GKA +DY+NDPL+RN+PW ++ ++AIGH+LQWHP +L +TA+
Sbjct: 299 GNIDASSFVIANDFYGKALIDYLNDPLLRNFPWDNTGDKLSAAIGHILQWHPTLLGKTAI 358
Query: 354 VVPKTIASVYSPSAVKK-----QADDAERFNYMDGDFVITFTGCEASKTCEIELDKHYQL 408
V+PK +AS Y S ++ A + + ++Y +GD +F GC + TC E+ YQ
Sbjct: 359 VIPKVLASQYDASLDQEGESGNGASNGDVYHYNEGDLAASFKGCRSRGTCASEIGHMYQK 418
Query: 409 VKQ 411
+K+
Sbjct: 419 IKK 421
>SPBC8D2.17 [GM] KOG4748 Subunit of Golgi mannosyltransferase complex
Length = 351
Score = 92.0 bits (227), Expect = 1e-18
Identities = 69/259 (26%), Positives = 121/259 (46%), Gaps = 24/259 (9%)
Query: 150 KNKANHPEVVIVTIIDFENY--------SLDHIINTVQNRVDYAQRHNYGTYVRWAQEFL 201
K P+ V++ I+ F+++ S + + +R +YA RH Y + A++
Sbjct: 57 KTSPQDPDSVVMLIVSFDDHYDSSRSDSSSVFLDKVLSDRTEYALRHGYTLVHKKARDIQ 116
Query: 202 PQVQKQSMEASYEFIKPLAVRAAMNAFPYAKYFWFFDQNGLIMQLDTSLQQQLLEPKVLD 261
+ + ++ I A+R + +P A + W D +IM SL+ ++L+P+ L
Sbjct: 117 A---RYGVYGTWSIIP--ALRETLAEYPDAGWIWLLDAKAVIMNPSESLKDRVLKPEKLS 171
Query: 262 AAVLKSVPVFEKSN-IKTYKHFDAKTAKIIIPKTGAGKLDTSSFVVTPSIHGKAFLDYIN 320
+L + P+ N I+T + D +I G + T S ++ + LD N
Sbjct: 172 QHLLLNSPIDPLKNYIRTRRKMDPSDVFVITTSDYNG-ISTRSLLIKNNNFAPFLLDAWN 230
Query: 321 DPLVRNYPWPSSFTSAIGHLLQWHPRILKRTALVVPKTIASVYSPSAVKKQADDAERFNY 380
+PL+++ + + SA+ HLL+ H IL ALV PK + S Y+ SAV NY
Sbjct: 231 EPLLKSAGFDQAERSALSHLLEAHNTILDHVALVSPKVLNS-YTNSAVD--------LNY 281
Query: 381 MDGDFVITFTGCEASKTCE 399
+GDF++ CE + CE
Sbjct: 282 EEGDFLVILQDCENAAACE 300
>SPAC22E12.06c [GM] KOG4748 Subunit of Golgi mannosyltransferase complex
Length = 332
Score = 90.1 bits (222), Expect = 5e-18
Identities = 69/264 (26%), Positives = 121/264 (45%), Gaps = 31/264 (11%)
Query: 157 EVVIVTIIDFE---NYSLDHIINTVQNRVDYAQRHNYG-TYVRWAQEFLPQVQKQSMEAS 212
E+VI+ D N + + ++NR++YA+ HNYG YV +Q +P V
Sbjct: 88 EIVILLASDGRSSGNMAPETFNQCIENRINYAKHHNYGFEYVNVSQMNIPPV-------- 139
Query: 213 YEFIKPLAVRAAMNAFPYAKYFWFFDQNGLIMQLDTSLQQQLLEPKVLDAAVLKSVPV-- 270
+ K A+ MN P+AK+ W+ DQ+ LI+ + S+Q+ +L P VL ++K+ P+
Sbjct: 140 --WAKMPAIIQTMNKHPHAKWIWWLDQDALILNTELSIQEHILSPDVLVEKLMKNEPMIS 197
Query: 271 -----FEKSNIKTYKHFDAKTAKIIIPKTGAGKLDTSSFVVTPSIHGKAFLDYINDPLVR 325
E+ +Y A++ ++I + G L+ SF V S FLD D R
Sbjct: 198 PFSADLERLTPSSYTVDSARSLGLLISQDLNG-LNAGSFFVRRSPMMALFLDLWGDKSFR 256
Query: 326 NYPWPSSFTSAIGHLLQWHPRILKRTALVVPKTIASVYSPSAVKKQADDAERFNYMDGDF 385
+ +G+ +++HP I ++P+T+ + Y + +G
Sbjct: 257 ENKVADHEQALLGYFVRYHPEIAAIIG-ILPQTLINSYPVG--------QPEMGWKEGHL 307
Query: 386 VITFTGCEASKTCEIELDKHYQLV 409
VI F GC C++ D++ + V
Sbjct: 308 VIHFAGCWVENRCQVLWDEYRERV 331
>SPBC1289.13c [GM] KOG4748 Subunit of Golgi mannosyltransferase complex
Length = 375
Score = 86.7 bits (213), Expect = 6e-17
Identities = 68/270 (25%), Positives = 123/270 (45%), Gaps = 28/270 (10%)
Query: 155 HPEVVIVTIIDFENYSLD-------HIINTVQNRVDYAQRHNYGTYVRWAQEFLPQV-QK 206
HPE + ++ N D +I ++NR DYA+RH + E+ P + K
Sbjct: 98 HPENSKIVLLMGSNAQNDPSSPLNPYIRTIIKNRRDYAERHGFKFEFLDLDEYKPSIGDK 157
Query: 207 QSMEASYEFIKPLAVRAAMNAFPYAKYFWFFDQNGLIMQLDTSLQQQLLEPKVLDAAVLK 266
+ A IK + + +P A++ W+ D + LIM D +L +L+ + L++ +L+
Sbjct: 158 PAPWAKIPMIKNV-----IRKYPDAEWVWWLDHDALIMNRDLNLVDHILKHEKLNSLLLR 212
Query: 267 SVPVF-----EKSNIKTYKHFDAKTAKIIIPKTGAGKLDTSSFVVTPSIHGKAFLDYIND 321
F + +T K+ D II + G ++ SF++ S G LD+ N+
Sbjct: 213 DTEYFSGFGIDSEGFRTPKNQDPDDIHFIIAQDFNG-INAGSFLIRNSEVGTWMLDFWNE 271
Query: 322 PLVRNYPWPSSFTSAIGHLLQWHPRILKRTALVVPKTIASVYSPSAVKKQADDAERFNYM 381
PL + + A+ H++ HP + K LV ++I + S +D A + Y
Sbjct: 272 PLYKEHNGVFVEQQALSHMIYSHPIVHKHVGLVTLRSINAYDS-------SDPA--WGYE 322
Query: 382 DGDFVITFTGCEASKTCEIELDKHYQLVKQ 411
DGD + F GC +TC +K+ +++ +
Sbjct: 323 DGDLCVHFAGCFVFQTCAQNFEKYGKIITE 352
>SPCC736.04c [GM] KOG4748 Subunit of Golgi mannosyltransferase complex
Length = 375
Score = 74.3 bits (181), Expect = 3e-13
Identities = 64/267 (23%), Positives = 113/267 (41%), Gaps = 26/267 (9%)
Query: 155 HPEVVIVTIIDFENYSLD-------HIINTVQNRVDYAQRHNYGTYVRWAQEFLPQVQKQ 207
HP+ + I+ N+ D + ++NR +YA+RH Y A + +V
Sbjct: 98 HPDNSKIVILMGSNFQNDANSPLHPFAQSIIKNRREYAERHGYKFEFLDADAYASRVTGH 157
Query: 208 SMEASYEFIKPLAVRAAMNAFPYAKYFWFFDQNGLIMQLDTSLQQQLLEPKVLDA----- 262
M ++K ++ M +P A++ W+ D + L+M D ++ +L+ L+
Sbjct: 158 LMP----WVKVPMLQDTMKKYPDAEWIWWLDHDALVMNKDLNVVDHVLKHDRLNTILTRE 213
Query: 263 AVLKSVPVFEKSNIKTYKHFDAKTAKIIIPKTGAGKLDTSSFVVTPSIHGKAFLDYINDP 322
A KS +T K DAK II + G ++ S + S G+ +D +P
Sbjct: 214 AEYKSGAGIPADGFRTPKDQDAKDVHFIISQDFNG-INAGSLFIRNSEVGRWIVDLWFEP 272
Query: 323 LVRNYPWPSSFTSAIGHLLQWHPRILKRTALVVPKTIASVYSPSAVKKQADDAERFNYMD 382
L ++ + A H++ +HP++ K +V K A+ D + Y D
Sbjct: 273 LYLDHIQGYAEQQAFSHMVFYHPQVYKHVGVVPLK---------AINAYDFDDNIWGYDD 323
Query: 383 GDFVITFTGCEASKTCEIELDKHYQLV 409
GD I F GC K C + K+ Q++
Sbjct: 324 GDLCIHFAGCNYFKNCPEKFLKYAQIL 350
>SPAC5H10.13c [GM] KOG4748 Subunit of Golgi mannosyltransferase complex
Length = 346
Score = 74.3 bits (181), Expect = 3e-13
Identities = 63/265 (23%), Positives = 111/265 (41%), Gaps = 34/265 (12%)
Query: 159 VIVTIIDFENYSLDHIINT----VQNRVDYAQRHNYG-TYVRWAQEFLPQVQKQSMEASY 213
+++ ++ + S ++ NT +QNRVDY+ + NY YV +P V
Sbjct: 101 IVLLLVSDGHTSYNNGANTFEEAIQNRVDYSTKQNYNFEYVNVTGLPIPAV--------- 151
Query: 214 EFIKPLAVRAAMNAFPYAKYFWFFDQNGLIMQLDTSLQQQLLEPK-----VLDAAVLKSV 268
+ K AV M +P A++ W DQ+ +I SLQ L+P+ ++ +L
Sbjct: 152 -WSKMPAVLQTMKKYPKAEWIWLLDQDAIITNTHLSLQDSFLKPENLQKTLITNTILTKR 210
Query: 269 PVFEKSNIK----TYKHFDAKTAKIIIPKTGAGKLDTSSFVVTPSIHGKAFLDYINDPLV 324
P+ +++ Y D + +II + G L+ S + S FLD DP+V
Sbjct: 211 PINANGDLRYTPSNYSLKDIENLMVIISQDHNG-LNAGSILFRNSPATALFLDIWTDPVV 269
Query: 325 RNYPWPSSFTSAIGHLLQWHPRILKRTALVVPKTIASVYSPSAVKKQADDAERFNYMDGD 384
++ +G+L+ H ++ L+ + I + + + E + GD
Sbjct: 270 AECAKANNEQDMLGYLISKHSQLASLVGLIPQRKINAFH---------EGPENMEWQKGD 320
Query: 385 FVITFTGCEASKTCEIELDKHYQLV 409
VI F GC C+ K Y L+
Sbjct: 321 LVIHFAGCWVENRCDELWQKFYALI 345
>SPAC5H10.11 [GM] KOG4748 Subunit of Golgi mannosyltransferase complex
Length = 329
Score = 70.1 bits (170), Expect = 6e-12
Identities = 60/259 (23%), Positives = 108/259 (41%), Gaps = 32/259 (12%)
Query: 151 NKANHPEVVIVTIID-----FENYSLDHIINTVQNRVDYAQRHNYGTYVRWAQEFLPQVQ 205
N+ E+V++ D FE+ LD + +NR++YA+ NY EF V
Sbjct: 80 NEPKSHEIVMLLASDGNVGSFESNLLD---DCFKNRIEYAKLQNYNF------EF---VN 127
Query: 206 KQSMEASYEFIKPLAVRAAMNAFPYAKYFWFFDQNGLIMQLDTSLQQQLLEPKVLDAAVL 265
S+ + K A+ M +P AK+ W+ DQ+ LIM + SLQ+ L P +L ++L
Sbjct: 128 VSSLVVPPVWGKMPAILQTMRKYPSAKWIWWLDQDALIMNKNLSLQELFLSPAMLQKSLL 187
Query: 266 KSVPVFEKSNIKTYKHFDAKTAKIIIPK------TGAGKLDTSSFVVTPSIHGKAFLDYI 319
+ P+ ++ A +K +I + L+ SF+V S +D +
Sbjct: 188 REQPIINSFGEDNFRITPAAYSKEMIEQIQFLISQDHNGLNAGSFLVRNSRSIALLMDLL 247
Query: 320 NDPLVRNYPWPSSFTSAIGHLLQWHPRILKRTALVVPKTIASVYSPSAVKKQADDAERFN 379
DP + + IG+ +Q H ++ ++ + I + + +
Sbjct: 248 TDPSLADAGVVRHEQDLIGYFIQKHSQVASMVGILPQRFINAFH---------EGPPTMQ 298
Query: 380 YMDGDFVITFTGCEASKTC 398
+ +GD + F GC C
Sbjct: 299 WQEGDLALHFAGCWVENKC 317
>YDR245w [GM] KOG4748 Subunit of Golgi mannosyltransferase complex
Length = 393
Score = 67.8 bits (164), Expect = 3e-11
Identities = 55/240 (22%), Positives = 107/240 (43%), Gaps = 12/240 (5%)
Query: 177 TVQNRVDYAQRHNYGTYVRWAQEFLPQVQKQSMEASYEFIKPLAVRAAMNAFPYAKYFWF 236
+++N+ YA+RH Y ++ L ++ S E + K +R FP A++FW+
Sbjct: 149 SIENKKAYAKRHGYALTIK----DLTTSKRYSHEYREGWQKVDILRQTFREFPNAEWFWW 204
Query: 237 FDQNGLIMQLDTSLQQQLLEPKVLDAAVLKSVPVFEKSNIK-TYKHFDAKTAKIIIPKTG 295
D + +IM+ SL++ + + L+ + + F N++ + D +
Sbjct: 205 LDLDTMIMEPSKSLEEHIFDR--LETLADRELKSFNPLNLRDDIPYVDYSEEMEFLITQD 262
Query: 296 AGKLDTSSFVVTPSIHGKAFLDYINDPLV---RNYPWPSSFTSAIGHLLQWHPRILKRTA 352
G + SF++ S K LD DP++ ++ W A+ L + P I R
Sbjct: 263 CGGFNLGSFLIKNSEWSKLLLDMWWDPVLYEQKHMVWEHREQDALEALYENEPWIRSRIG 322
Query: 353 LVVPKTIASVYSPSAVKKQADDAERF-NYMDGDFVITFTGCEASKTCEIELDKHYQLVKQ 411
+ +TI + + P A + + D+ F + D DFV+ GC + C E+ + L+++
Sbjct: 323 FLPLRTI-NAFPPGACSEYSGDSRYFYSEKDHDFVVNMAGCNFGRDCWGEMQYYTTLMEK 381
>SPAC637.06 [GM] KOG4748 Subunit of Golgi mannosyltransferase complex
Length = 347
Score = 66.2 bits (160), Expect = 8e-11
Identities = 58/242 (23%), Positives = 105/242 (42%), Gaps = 15/242 (6%)
Query: 177 TVQNRVDYAQRHNYGTYVRWAQEFLPQVQKQSMEASYEFIKPLAVRAAMNAFPYAKYFWF 236
+ +N+ YA+RH Y +V+ L + S+E K + AM +P+A++FW+
Sbjct: 102 STENKKKYAERHGYHFFVK--STGLKRRYAHEWRESWE--KADYIMEAMKKYPHAEWFWW 157
Query: 237 FDQNGLIMQLDTSLQQQLLEPKVLDAAVLKSVP---VFEKSNIKTYKHFDAKTAKIIIPK 293
D IM+ SL++ ++ LD +++ F N D I
Sbjct: 158 VDLTTFIMEPQYSLEKLIINR--LDHIATRNITDSMEFNPKNFHEIPFVDYSEDINFILG 215
Query: 294 TGAGKLDTSSFVVTPSIHGKAFLDYINDPLV---RNYPWPSSFTSAIGHLLQWHPRILKR 350
SF+V S +D++ DP+V ++ WP +AI + + + L+
Sbjct: 216 QDCNGFSLGSFLVRRSDWTSRLMDFLWDPVVYGQKHMDWPHDEQNAIEYFYE-NNAWLRS 274
Query: 351 TALVVPKTIASVYSPSAVKKQADDAERF-NYMDGDFVITFTGCEASKTCEIELDKHYQLV 409
VP + + Y P A + ++ F N +GDF++ GC + C E++ HY+ +
Sbjct: 275 GMGFVPLRLFNSYPPGACAGEGENPRFFYNQKEGDFLVNLAGCNYGRDCLDEIN-HYKGL 333
Query: 410 KQ 411
Q
Sbjct: 334 AQ 335
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.136 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,856,697
Number of Sequences: 60738
Number of extensions: 1064288
Number of successful extensions: 2704
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 2679
Number of HSP's gapped (non-prelim): 11
length of query: 412
length of database: 30,389,216
effective HSP length: 109
effective length of query: 303
effective length of database: 23,768,774
effective search space: 7201938522
effective search space used: 7201938522
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)