ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI1848 good R KOG3165 General function prediction only Predicted nucleic-acid-binding protein, contains PIN domain
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI1848 633092 632526 -189
(189 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR339c [R] KOG3165 Predicted nucleic-acid-binding protein conta... 349 1e-96
SPBC32H8.04c [R] KOG3165 Predicted nucleic-acid-binding protein ... 239 1e-63
At2g46230 [R] KOG3165 Predicted nucleic-acid-binding protein con... 238 5e-63
Hs7705730 [R] KOG3165 Predicted nucleic-acid-binding protein con... 237 6e-63
CE09810 [R] KOG3165 Predicted nucleic-acid-binding protein conta... 196 1e-50
At1g26530 [R] KOG3165 Predicted nucleic-acid-binding protein con... 179 3e-45
Hs17435387 [R] KOG3165 Predicted nucleic-acid-binding protein co... 153 1e-37
ECU05g0560 [R] KOG3165 Predicted nucleic-acid-binding protein co... 150 8e-37
7297567 [R] KOG3165 Predicted nucleic-acid-binding protein conta... 149 2e-36
CE27743 [R] KOG3164 Uncharacterized proteins of PilT N-term./Vap... 75 7e-14
Hs14150118 [R] KOG3164 Uncharacterized proteins of PilT N-term./... 69 4e-12
7296046 [R] KOG3164 Uncharacterized proteins of PilT N-term./Vap... 63 2e-10
SPCC18.12c [R] KOG3164 Uncharacterized proteins of PilT N-term./... 60 2e-09
YOR004w [R] KOG3164 Uncharacterized proteins of PilT N-term./Vap... 51 8e-07
At2g34570 [R] KOG3164 Uncharacterized proteins of PilT N-term./V... 50 2e-06
>YDR339c [R] KOG3165 Predicted nucleic-acid-binding protein contains PIN
domain
Length = 189
Score = 349 bits (895), Expect = 1e-96
Identities = 169/189 (89%), Positives = 178/189 (93%)
Query: 1 MGKAKKTRKFALVKRTLNAKKDSRLKSNQAKEVKKDDPELTKNIPQVSSALFFQYNEAIK 60
MGKAKKTRKF LVKRTLN KKD RLK NQ K+DPELT+NIPQVSSALFFQYN+AIK
Sbjct: 1 MGKAKKTRKFGLVKRTLNTKKDQRLKKNQENIKTKEDPELTRNIPQVSSALFFQYNQAIK 60
Query: 61 PPYQVLIDTNFINFSIQKKIDIVKGMMDCLLAKCNILITDCVMAELEKLGPKYRIALKLA 120
PPYQVLIDTNFINFSIQKK+DIV+GMMDCLLAKCN LITDCVMAELEKLGPKYRIALKLA
Sbjct: 61 PPYQVLIDTNFINFSIQKKVDIVRGMMDCLLAKCNPLITDCVMAELEKLGPKYRIALKLA 120
Query: 121 RDPRIKRLSCSHKGTYADDCIVHRVLQHKCYIVATNDAGLKQRVRKVPGIPLMSVGGHSY 180
RDPRIKRLSCSHKGTYADDC+VHRVLQHKCYIVATNDAGLKQR+RK+PGIPLMSVGGH+Y
Sbjct: 121 RDPRIKRLSCSHKGTYADDCLVHRVLQHKCYIVATNDAGLKQRIRKIPGIPLMSVGGHAY 180
Query: 181 VIEKLPGVF 189
VIEKLP VF
Sbjct: 181 VIEKLPDVF 189
>SPBC32H8.04c [R] KOG3165 Predicted nucleic-acid-binding protein contains PIN
domain
Length = 192
Score = 239 bits (611), Expect = 1e-63
Identities = 116/190 (61%), Positives = 147/190 (77%), Gaps = 2/190 (1%)
Query: 1 MGKAKKTRKFALVKRTLNAKKDSRLKSNQAKEVKKD--DPELTKNIPQVSSALFFQYNEA 58
MGKAK TRKFA VKR +N K K +Q KE +K + EL + IPQ++S LFFQ+NE+
Sbjct: 1 MGKAKTTRKFAQVKRVINLKDQRLQKKDQKKEKEKTTKNGELVREIPQMASNLFFQFNES 60
Query: 59 IKPPYQVLIDTNFINFSIQKKIDIVKGMMDCLLAKCNILITDCVMAELEKLGPKYRIALK 118
+ PPY V+IDTNFINF +Q+KID+ +G+M CL AK I+DCVMAELEKLG +YRIAL+
Sbjct: 61 LGPPYHVIIDTNFINFCLQQKIDLFEGLMTCLYAKTIPCISDCVMAELEKLGIRYRIALR 120
Query: 119 LARDPRIKRLSCSHKGTYADDCIVHRVLQHKCYIVATNDAGLKQRVRKVPGIPLMSVGGH 178
+A+D R +RL C+HKGTYADDCIV RV+QHKCY+VATND LKQR+RK+PGIP++SV H
Sbjct: 121 IAKDERFERLPCTHKGTYADDCIVQRVMQHKCYLVATNDKNLKQRIRKIPGIPILSVANH 180
Query: 179 SYVIEKLPGV 188
+E+L V
Sbjct: 181 KIRVERLVDV 190
>At2g46230 [R] KOG3165 Predicted nucleic-acid-binding protein contains PIN
domain
Length = 216
Score = 238 bits (606), Expect = 5e-63
Identities = 113/186 (60%), Positives = 137/186 (72%)
Query: 1 MGKAKKTRKFALVKRTLNAKKDSRLKSNQAKEVKKDDPELTKNIPQVSSALFFQYNEAIK 60
MGKAKK +KFA+VK+ ++ K K KKD EL +N+P V + LFF YN +
Sbjct: 1 MGKAKKPQKFAVVKKMISHKALKHYKEEVLNPNKKDLTELPRNVPSVPAGLFFSYNSTLV 60
Query: 61 PPYQVLIDTNFINFSIQKKIDIVKGMMDCLLAKCNILITDCVMAELEKLGPKYRIALKLA 120
PPY+VL+DTNFINFSIQ KID+ KGM DCL A C ITDCVMAELEKLG KYR+AL++A
Sbjct: 61 PPYRVLVDTNFINFSIQNKIDLEKGMRDCLYANCTPCITDCVMAELEKLGQKYRVALRIA 120
Query: 121 RDPRIKRLSCSHKGTYADDCIVHRVLQHKCYIVATNDAGLKQRVRKVPGIPLMSVGGHSY 180
+DP +RL C HKGTYADDC+V RV QHKC+IVAT D LK+R+RK+PG+P+M V Y
Sbjct: 121 KDPHFERLPCIHKGTYADDCLVDRVTQHKCFIVATCDRDLKRRIRKIPGVPIMYVTNRKY 180
Query: 181 VIEKLP 186
IEKLP
Sbjct: 181 SIEKLP 186
>Hs7705730 [R] KOG3165 Predicted nucleic-acid-binding protein contains PIN
domain
Length = 198
Score = 237 bits (605), Expect = 6e-63
Identities = 114/191 (59%), Positives = 145/191 (75%), Gaps = 6/191 (3%)
Query: 1 MGKAKKTRKFALVKRTLNAKKDSRLKSN---QAKEVKKDDPELTKN--IPQVSSALFFQY 55
MGK KKTRK+A +KR L+ + D RLK + K+ +K DP K +PQ S LFFQY
Sbjct: 1 MGKQKKTRKYATMKRMLSLR-DQRLKEKDRLKPKKKEKKDPSALKEREVPQHPSCLFFQY 59
Query: 56 NEAIKPPYQVLIDTNFINFSIQKKIDIVKGMMDCLLAKCNILITDCVMAELEKLGPKYRI 115
N + PPY +L+DTNFINFSI+ K+D+V+ MMDCL AKC ITDCVMAE+EKLG KYR+
Sbjct: 60 NTQLGPPYHILVDTNFINFSIKAKLDLVQSMMDCLYAKCIPCITDCVMAEIEKLGQKYRV 119
Query: 116 ALKLARDPRIKRLSCSHKGTYADDCIVHRVLQHKCYIVATNDAGLKQRVRKVPGIPLMSV 175
AL++A+DPR +RL C+HKGTYADDC+V RV QHKCYIVAT D LK+R+RK+PG+P+M +
Sbjct: 120 ALRIAKDPRFERLPCTHKGTYADDCLVQRVTQHKCYIVATVDRDLKRRIRKIPGVPIMYI 179
Query: 176 GGHSYVIEKLP 186
H Y IE++P
Sbjct: 180 SNHRYNIERMP 190
>CE09810 [R] KOG3165 Predicted nucleic-acid-binding protein contains PIN
domain
Length = 196
Score = 196 bits (499), Expect = 1e-50
Identities = 93/192 (48%), Positives = 134/192 (69%), Gaps = 4/192 (2%)
Query: 1 MGKAKKTRKFALVKRTLNAKKDSRLKSNQA--KEVKKDDPELTK-NIPQVSSALFFQYNE 57
MGK K+ L K D+R+K Q + K D+ EL P++SSA+F +YN
Sbjct: 1 MGKRLKSNARKSAVNKLVKKTDNRIKEEQRVIRTPKVDEQELKLVRAPKISSAMFMKYNT 60
Query: 58 AIKPPYQVLIDTNFINFSIQKKIDIVKGMMDCLLAKCNILITDCVMAELEKLGPKYRIAL 117
+ PP+ V++DTNF+NF+++ +ID+ +G MDCL AK + TDCV+AELEK+ +++IAL
Sbjct: 61 QLGPPFHVIVDTNFVNFAVKNRIDMFQGFMDCLFAKTIVYATDCVLAELEKVR-RFKIAL 119
Query: 118 KLARDPRIKRLSCSHKGTYADDCIVHRVLQHKCYIVATNDAGLKQRVRKVPGIPLMSVGG 177
K+ +DPR++RL C HKGTYADDC+V RV QHKCYIVAT D LK+R+RK+PG+P+M +
Sbjct: 120 KVLKDPRVQRLKCEHKGTYADDCLVQRVTQHKCYIVATCDRDLKRRIRKIPGVPIMYIVN 179
Query: 178 HSYVIEKLPGVF 189
H + IE++P +
Sbjct: 180 HRFSIERMPDAY 191
>At1g26530 [R] KOG3165 Predicted nucleic-acid-binding protein contains PIN
domain
Length = 189
Score = 179 bits (453), Expect = 3e-45
Identities = 92/186 (49%), Positives = 117/186 (62%), Gaps = 26/186 (13%)
Query: 1 MGKAKKTRKFALVKRTLNAKKDSRLKSNQAKEVKKDDPELTKNIPQVSSALFFQYNEAIK 60
MG+ KK +KFA++K ++ K K KKD EL +N+P V S LFF +N ++
Sbjct: 1 MGRTKKPQKFAVMKMMISHKALKHYKEEVLNPNKKDLTELPRNVPSVPSGLFFSHNSSLV 60
Query: 61 PPYQVLIDTNFINFSIQKKIDIVKGMMDCLLAKCNILITDCVMAELEKLGPKYRIALKLA 120
PPY+VL+DTNFINFSIQ K LEKLG KYR+AL++A
Sbjct: 61 PPYRVLVDTNFINFSIQNK--------------------------LEKLGQKYRVALRIA 94
Query: 121 RDPRIKRLSCSHKGTYADDCIVHRVLQHKCYIVATNDAGLKQRVRKVPGIPLMSVGGHSY 180
+DPR +RL C KGTYADDC+V RV QHKC+IVAT D LK+R+RK+PG+P+M V Y
Sbjct: 95 KDPRFERLPCVLKGTYADDCLVDRVTQHKCFIVATCDRDLKRRIRKIPGVPIMYVTQRKY 154
Query: 181 VIEKLP 186
IEKLP
Sbjct: 155 SIEKLP 160
>Hs17435387 [R] KOG3165 Predicted nucleic-acid-binding protein contains PIN
domain
Length = 136
Score = 153 bits (387), Expect = 1e-37
Identities = 73/129 (56%), Positives = 95/129 (73%), Gaps = 5/129 (3%)
Query: 21 KDSRLKSN---QAKEVKKDDPELTKN--IPQVSSALFFQYNEAIKPPYQVLIDTNFINFS 75
+D RLK + K+ +K DP K +PQ S LFFQYN + PPY +LIDTNFINFS
Sbjct: 8 RDQRLKEKDRLKPKKKEKKDPNTLKEREVPQHPSHLFFQYNTQLGPPYHILIDTNFINFS 67
Query: 76 IQKKIDIVKGMMDCLLAKCNILITDCVMAELEKLGPKYRIALKLARDPRIKRLSCSHKGT 135
I+ K+D+++ MMDCL AKC ITDCVMAE+EKLG KYR+AL++ +DPR + L C+H+GT
Sbjct: 68 IKAKLDLMQSMMDCLYAKCIPCITDCVMAEIEKLGQKYRVALRITKDPRFELLPCAHQGT 127
Query: 136 YADDCIVHR 144
YADDC+V +
Sbjct: 128 YADDCLVQK 136
>ECU05g0560 [R] KOG3165 Predicted nucleic-acid-binding protein contains PIN
domain
Length = 179
Score = 150 bits (380), Expect = 8e-37
Identities = 76/173 (43%), Positives = 108/173 (61%), Gaps = 11/173 (6%)
Query: 14 KRTLNA--KKDSRLKSNQAKEVKKDDPELTKNIPQVSSALFFQYNEAIKPPYQVLIDTNF 71
KR N +K +R+KS + ++P T++ F N ++PPY +++DTNF
Sbjct: 10 KRVANVWDRKKTRVKSPENASSTGENPRNTED---------FLLNHTLRPPYNIILDTNF 60
Query: 72 INFSIQKKIDIVKGMMDCLLAKCNILITDCVMAELEKLGPKYRIALKLARDPRIKRLSCS 131
IN I+KK DI + +M + A I I DCV+ ELE LG +R+AL RD ++RL C
Sbjct: 61 INDCIRKKYDIREQLMKAVNASIKICIPDCVIGELESLGRPFRVALASIRDSDVQRLECD 120
Query: 132 HKGTYADDCIVHRVLQHKCYIVATNDAGLKQRVRKVPGIPLMSVGGHSYVIEK 184
HKGTYADDCI +RV H+CYIVAT+D LKQR++ +PG+PL++ G IE+
Sbjct: 121 HKGTYADDCIFNRVSAHRCYIVATSDVALKQRIKAIPGVPLITYRGQRCFIER 173
>7297567 [R] KOG3165 Predicted nucleic-acid-binding protein contains PIN
domain
Length = 107
Score = 149 bits (376), Expect = 2e-36
Identities = 66/103 (64%), Positives = 80/103 (77%)
Query: 87 MDCLLAKCNILITDCVMAELEKLGPKYRIALKLARDPRIKRLSCSHKGTYADDCIVHRVL 146
MDCL AKC I+DCV AELEKLG KY++AL++ DPR +RL C HKGTYADDC+V RV
Sbjct: 1 MDCLYAKCIPYISDCVRAELEKLGNKYKLALRIISDPRFERLPCLHKGTYADDCLVERVR 60
Query: 147 QHKCYIVATNDAGLKQRVRKVPGIPLMSVGGHSYVIEKLPGVF 189
QHKCYIVATND LK R+RK+PG+P+M V H Y IE++P +
Sbjct: 61 QHKCYIVATNDKDLKNRIRKIPGVPIMYVAAHKYAIERMPEAY 103
>CE27743 [R] KOG3164 Uncharacterized proteins of PilT N-term./Vapc
superfamily
Length = 232
Score = 74.7 bits (182), Expect = 7e-14
Identities = 42/139 (30%), Positives = 77/139 (55%), Gaps = 7/139 (5%)
Query: 52 FFQYNEAIKPPYQVLIDTNFINFSIQKKIDIVKGMMDCLLAKCNILITDCVMAELEKLGP 111
F++YN PY+VL+D F N ++Q+K+++ + + L + +++ T+CV+ ELEK GP
Sbjct: 15 FYKYNYKFVAPYRVLVDGTFCNAALQEKLNLAEQIPKYLTEETHLMTTNCVLKELEKFGP 74
Query: 112 KYRIALKLARDPRIKRLSCSHK-GTYADDCIVH---RVLQHKC-YIVATNDAGLKQRVRK 166
A +A+ I C+H A DC+ H R K Y++AT D L +++R
Sbjct: 75 LLYGAFVIAKQFEI--AECTHSTPRAASDCLAHLARRAASGKTKYLIATQDDELTEKLRA 132
Query: 167 VPGIPLMSVGGHSYVIEKL 185
+ G P+M + + +++ +
Sbjct: 133 IVGTPIMYIKFKTVLLDNI 151
>Hs14150118 [R] KOG3164 Uncharacterized proteins of PilT N-term./Vapc
superfamily
Length = 249
Score = 68.9 bits (167), Expect = 4e-12
Identities = 38/135 (28%), Positives = 71/135 (52%), Gaps = 2/135 (1%)
Query: 52 FFQYNEAIKPPYQVLIDTNFINFSIQKKIDIVKGMMDCLLAKCNILITDCVMAELEKLGP 111
FF+ N ++ PYQ+L+D F +++ +I + + + L+ + + T CV+ ELE LG
Sbjct: 15 FFRNNFGVREPYQILLDGTFCQAALRGRIQLREQLPRYLMGETQLCTTRCVLKELETLGK 74
Query: 112 KYRIALKLARDPRIKRLSCSHKGTYADDCIVHRVLQHK--CYIVATNDAGLKQRVRKVPG 169
A +A+ +++ +C++ V + Y VAT D L +V+K PG
Sbjct: 75 DLYGAKLIAQKCQVRNCPHFKNAVSGSECLLSMVEEGNPHHYFVATQDQNLSVKVKKKPG 134
Query: 170 IPLMSVGGHSYVIEK 184
+PLM + ++ V++K
Sbjct: 135 VPLMFIIQNTMVLDK 149
>7296046 [R] KOG3164 Uncharacterized proteins of PilT N-term./Vapc
superfamily
Length = 244
Score = 63.2 bits (152), Expect = 2e-10
Identities = 43/135 (31%), Positives = 68/135 (49%), Gaps = 5/135 (3%)
Query: 51 LFFQYNEAIKPPYQVLIDTNFINFSIQKKIDIVKGMMDCLLAKCNILITDCVMAELEKLG 110
+FF N + PYQVLID F ++Q+KI I + + +L T CV+ E E LG
Sbjct: 14 VFFASNFDYREPYQVLIDATFCQAALQQKIGIDEQIKKYFQCGVKLLTTQCVILESESLG 73
Query: 111 PKYRIALKLARDPRIKRLSCSHKG--TYADDCIVHRVLQHKCYIVATNDAGLKQRVRKVP 168
A + + R C H+G A +CI ++ Y+VA+ D L++ +RK+P
Sbjct: 74 APLTGATSIVK--RFHVHKCGHEGKPVPASECIKSMTKDNR-YVVASQDRLLQESLRKIP 130
Query: 169 GIPLMSVGGHSYVIE 183
G L+ + + V+E
Sbjct: 131 GRCLLYLHKATPVLE 145
>SPCC18.12c [R] KOG3164 Uncharacterized proteins of PilT N-term./Vapc
superfamily
Length = 260
Score = 60.1 bits (144), Expect = 2e-09
Identities = 38/138 (27%), Positives = 70/138 (50%), Gaps = 10/138 (7%)
Query: 53 FQYNEAIKPPYQVLIDTNFINFSIQKKIDIVKGMMDCLLAKCNILITDCVMAELEKLGPK 112
+Q + PYQVL+D +F+ Q+KIDI + + +IT C + +L +
Sbjct: 16 YQLLFGFREPYQVLVDADFLKDLSQQKIDIQAALARTVQGAIKPMITQCCIRQLYSKSDE 75
Query: 113 YRIALKLARDPRIKRLSCSH--KGTYADDCI-----VHRVLQHKCYIVATNDAGLKQRVR 165
+ +++A+ +R C H + +CI ++ +H+ Y+VAT D L+Q +R
Sbjct: 76 LKQEIRIAKS--FERRRCGHIDEALSPSECIQSVVNINGRNKHR-YVVATQDPELRQALR 132
Query: 166 KVPGIPLMSVGGHSYVIE 183
VPG+PL+ + ++E
Sbjct: 133 SVPGVPLIYMKRSVVILE 150
>YOR004w [R] KOG3164 Uncharacterized proteins of PilT N-term./Vapc
superfamily
Length = 254
Score = 51.2 bits (121), Expect = 8e-07
Identities = 37/141 (26%), Positives = 69/141 (48%), Gaps = 9/141 (6%)
Query: 51 LFFQYNEAIKPPYQVLIDTNFINFSIQKKIDIVKGMMDCLLAKCNILITDCVMAELEKLG 110
L + + + PYQVL+D + ++ G+ L A ++IT C + L +
Sbjct: 14 LVYSHTFKFREPYQVLVDNQLVLECNNSNFNLPSGLKRTLQADVKVMITQCCIQALYET- 72
Query: 111 PKYRIALKLARDPRIKRLSCSHKGTYAD-DCI-----VHRVLQHKCYIVATNDAGLKQRV 164
+ A+ LA+ +R + S K + +CI + +H+ Y+VA+ D L++++
Sbjct: 73 -RNDGAINLAKQFERRRCNHSFKDPKSPAECIESVVNISGANKHR-YVVASQDIDLRRKL 130
Query: 165 RKVPGIPLMSVGGHSYVIEKL 185
R VPG+PL+ + V+E L
Sbjct: 131 RTVPGVPLIHLTRSVMVMEPL 151
>At2g34570 [R] KOG3164 Uncharacterized proteins of PilT N-term./Vapc
superfamily
Length = 258
Score = 49.7 bits (117), Expect = 2e-06
Identities = 35/126 (27%), Positives = 64/126 (50%), Gaps = 6/126 (4%)
Query: 52 FFQYNEAIKPPYQVLIDTNFINFSIQKKIDIVKGMMDCLLA-KCNILITDCVMAELEKLG 110
FF + PY+VL D F++ + +I + LL + T CV+AELEKLG
Sbjct: 15 FFTVCYGFRQPYKVLCDGTFVHHLVTNEITPADTAVSELLGGPVKLFTTRCVIAELEKLG 74
Query: 111 PKYRIALKLARDPRIKRLSCSH-KGTYADDCI--VHRVLQHKCYIVATNDAGLKQRVRKV 167
+ +L+ A+ + +C H + AD+C+ V V + + + T DA ++++++
Sbjct: 75 KDFAESLEAAQ--TLNTATCEHEEAKTADECLSEVIGVQNTEHFFLGTQDAEFRRKLQQE 132
Query: 168 PGIPLM 173
+PL+
Sbjct: 133 SIVPLV 138
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.322 0.138 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,950,446
Number of Sequences: 60738
Number of extensions: 429341
Number of successful extensions: 1227
Number of sequences better than 1.0e-05: 15
Number of HSP's better than 0.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1207
Number of HSP's gapped (non-prelim): 15
length of query: 189
length of database: 30,389,216
effective HSP length: 100
effective length of query: 89
effective length of database: 24,315,416
effective search space: 2164072024
effective search space used: 2164072024
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)