ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI1870 good S KOG1338 Function unknown Uncharacterized conserved protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI1870 638900 640480 527
(527 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR257c [S] KOG1338 Uncharacterized conserved protein 351 2e-96
SPAC688.14 [S] KOG1338 Uncharacterized conserved protein 100 4e-21
At3g56570 [R] KOG1337 N-methyltransferase 71 5e-12
Hs13376288 [S] KOG1338 Uncharacterized conserved protein 61 5e-09
At1g14030 [R] KOG1337 N-methyltransferase 50 8e-06
>YDR257c [S] KOG1338 Uncharacterized conserved protein
Length = 494
Score = 351 bits (900), Expect = 2e-96
Identities = 193/507 (38%), Positives = 299/507 (58%), Gaps = 39/507 (7%)
Query: 5 DFERKTDLFLEWLPTVG-IKLSKNVKIVDSRDEHQGRSMICINDVKEGEKLFSVPETASL 63
DF R T+ F+ WL T I++S ++I D ++QGR+++ +K+ E LF +P ++ L
Sbjct: 3 DFSRDTENFVCWLKTTAEIEVSPKIEIKDLCCDNQGRAVVATQKIKKDETLFKIPRSSVL 62
Query: 64 NIITGSLGKLDESYLGILLSKVEHWHGLILTILYEWKYLGEKSKWWPYFQVL--PDQFDT 121
++ T L K S L++ W GLI+ ILYE + L E+S+W PYF+V P +
Sbjct: 63 SVTTSQLIKDYPSLKDKFLNETGSWEGLIICILYEMEVLQERSRWAPYFKVWNKPSDMNA 122
Query: 122 LIYWDKEDLDKLKPSLVLDRLGEQQSKDMYVKVLELMKTFNVADKIGEVT----YDDFQR 177
LI+WD +L LKPSLVL+R+G++++K+M+ ++++ +K + + V +D+F
Sbjct: 123 LIFWDDNELQLLKPSLVLERIGKKEAKEMHERIIKSIK--QIGGEFSRVATSFEFDNFAY 180
Query: 178 VASWIMAYSFDKEITAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNS 237
+AS I++YSFD E+
Sbjct: 181 IASIILSYSFDLEMQ-----------------------------DSSVNENEEEETSEEE 211
Query: 238 VNFDGVIKCMIAVADILNSDTNLVNANIIYDKTGLICCATKDIKAGEQVYNIYGNHPNSE 297
+ + +K MI +AD+LN+DT+ NAN+ YD L A +DI+ EQVYNIYG HPNSE
Sbjct: 212 LENERYLKSMIPLADMLNADTSKCNANLTYDSNCLKMVALRDIEKNEQVYNIYGEHPNSE 271
Query: 298 ILRRYGYVEWTGSKYDFGEVTLKNILDSLSEKYNVSTEFLDRVVEILREDEKIKEIWDYE 357
+LRRYGYVEW GSKYDFGEV L+NI+++L E + +TEFLDR ++ILR + I+E + E
Sbjct: 272 LLRRYGYVEWDGSKYDFGEVLLENIVEALKETFETNTEFLDRCIDILRNNANIQEFLEGE 331
Query: 358 DVVLDSYDCYADGETIPEAASTIQILFVLLQSHEVVKLEELALTRLLQRIVKKCQQLLES 417
++VLDSYDCY +GE +P+ +QIL +L Q + KL+ A+ R ++RIVKKC QL+E
Sbjct: 332 EIVLDSYDCYNNGELLPQLILLVQILTILCQIPGLCKLDIKAMERQVERIVKKCLQLIEG 391
Query: 418 GRVTESVNSIWQQAVEKRLKMYPQTNPVLSEDRIKETFAKSKSDQRQLMASCILECERRC 477
R T + ++ W++ + KRL YP +S ++ + + ++ + R +MA +L+ E
Sbjct: 392 ARATTNCSATWKRCIMKRLADYP-IKKCVSIEKPSKGNSLTREELRDVMARRVLKSEIDS 450
Query: 478 IAKSREGISKQFKLLDDEKLLKNVLKR 504
+ E I K +K++ DEKLL N+LKR
Sbjct: 451 LQVCEETIDKNYKVIPDEKLLTNILKR 477
>SPAC688.14 [S] KOG1338 Uncharacterized conserved protein
Length = 461
Score = 100 bits (250), Expect = 4e-21
Identities = 87/350 (24%), Positives = 151/350 (42%), Gaps = 39/350 (11%)
Query: 25 SKNVKIVDSRDEHQ--GRSMICINDVKEGEKLFSVPETASLNIITGSLGKLDESYLGILL 82
S+ + + D R H+ G I + D+KE EKL S + + L + L +L E +
Sbjct: 14 SEKIGLNDYRHVHESLGIGFIALEDIKEDEKLVSFKKDSVLCLTNSDLAQLPE------V 67
Query: 83 SKVEHWHGLILTILYEWKYLGEKSKWWPYFQVLP--DQFDTLIYWDKEDLDKLKPSLVLD 140
+ W L+L + E S W PY + P ++ + YWD+ D L VL+
Sbjct: 68 QSLPSWAALLLVMATE--NASPNSFWRPYLSIFPTKERITSPFYWDENKKDALLRGTVLE 125
Query: 141 RLGE-QQSKDMYVKVLE-LMKTFNVADKIGEVTYDDFQRVASWIMAYSFDKEITAXXXXX 198
+ + +++ +E ++K + ++ +V+Y+DF R+++ ++AYSFD E T
Sbjct: 126 SNEDCNEITQLWIDRIEPIIKLY--PNRFSQVSYEDFLRMSAVMLAYSFDIEKTKSPISN 183
Query: 199 XXXXXXXXXXXXXXXXXXXXXXXXXXXX-------------------XXXXXXXXXNSVN 239
+ V+
Sbjct: 184 ENEKSAAETSIKEDKNGDAAKKNEGSANQDDEKLHSQSLVGNNCEVNSEDEFSDLESEVD 243
Query: 240 FDGVIKCMIAVADILNSDTNLVNANIIYDKTG-LICCATKDIKAGEQVYNIYGNHPNSEI 298
D + K M ++D+ N D L N + YD G L AT+DIK GEQ++N YG NSE+
Sbjct: 244 PDELEKAMCPISDMFNGDDELCNIRL-YDINGTLTMIATRDIKKGEQLWNTYGELDNSEL 302
Query: 299 LRRYGYVEWTGSKYDFGEVTLKNILDSLSEKYNVSTEFLDRVVEILREDE 348
R+YG+ + G+ +DF + ++ L EK + E ++ +E+L +E
Sbjct: 303 FRKYGFTKKKGTPHDFVLIKKEHWLPEYIEK--LGFEEVEARLELLCREE 350
>At3g56570 [R] KOG1337 N-methyltransferase
Length = 531
Score = 70.9 bits (172), Expect = 5e-12
Identities = 46/144 (31%), Positives = 76/144 (51%), Gaps = 9/144 (6%)
Query: 8 RKTDLFLEWLPTVGIKLSKNVKIVDSRDEHQGRSMICINDVKEGEKLFSVPETASLNIIT 67
R+ F W+ G+ S+ + +VD DE+ G S+ D+KEG+ + ++ +TA L I T
Sbjct: 4 RRLRAFKRWMQANGVDCSEALNLVD--DENDGVSVRAFCDLKEGDVVANISKTACLTIKT 61
Query: 68 GSLGKLDESYLGILLSKVEHWHGLILTILYEWKYLGEKSKWWPYFQVLPDQFDTLIYWDK 127
++ ES + ++ GL + ++YE + LGE+S W Y Q+LP Q D + W
Sbjct: 62 SGAREMIES------ADLDGSLGLSVALMYE-RSLGEESPWAGYLQILPIQEDLPLVWSL 114
Query: 128 EDLDKLKPSLVLDRLGEQQSKDMY 151
EDLD L L +L ++ +Y
Sbjct: 115 EDLDSLLSGTELHKLVKEDHVLIY 138
>Hs13376288 [S] KOG1338 Uncharacterized conserved protein
Length = 449
Score = 60.8 bits (146), Expect = 5e-09
Identities = 70/301 (23%), Positives = 117/301 (38%), Gaps = 55/301 (18%)
Query: 13 FLEWLPTVGIKLSKNVKIVDSRDEHQGRSMICINDVKEGEKLFSVPETASLNIITGSLGK 72
FL W VG++LS V V + G M+ V+ GE LF VP A L+ T S+G
Sbjct: 25 FLSWCRRVGLELSPKVA-VSRQGTVAGYGMVARESVQAGELLFVVPRAALLSQHTCSIGG 83
Query: 73 LDESYLGILLSKVEHWHGLILTILYEWKYLGEKSKWWPYFQVLPD--QFDTLIYWDKEDL 130
L E + L W L+L +L+E + S+W PYF + P+ + + ++W +E+
Sbjct: 84 LLERE-RVALQGQSGWVPLLLALLHELQ--APASRWRPYFALWPELGRLEHPMFWPEEER 140
Query: 131 DKL-----KPSLVLDRLGEQQSKDMYVKVLELMKTFNVADKIGEVTYDDFQRVASWIMAY 185
L P V L +S+ + VL M+ + + + + ++ + +MAY
Sbjct: 141 RCLLQGTGVPEAVEKDLANIRSEYQSI-VLPFMEAHPDLFSLRVRSLELYHQLVALVMAY 199
Query: 186 SFDKEITAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNSVNFDGVIK 245
SF + N ++
Sbjct: 200 SFQVPL--------------------------------------EEEEDEKEPNSPVMVP 221
Query: 246 CMIAVADILNSDTNLVNANIIYDKTGLICCATKDIKAGEQVYNIYGNHPNSEILRRYGYV 305
+ + N + NL Y L AT+ I G +++N YG N +++ YG+V
Sbjct: 222 AADILNHLANHNANLE-----YSANCLRMVATQPIPKGHEIFNTYGQMANWQLIHMYGFV 276
Query: 306 E 306
E
Sbjct: 277 E 277
>At1g14030 [R] KOG1337 N-methyltransferase
Length = 482
Score = 50.1 bits (118), Expect = 8e-06
Identities = 41/165 (24%), Positives = 80/165 (47%), Gaps = 11/165 (6%)
Query: 13 FLEWLPTVGIKLSKNVKIVDSRDEHQGRSMICINDVKEGEKLFSVPETASLNIITGSLGK 72
F +WL G+ K+V + +G ++ D+ E + +P+ +N T + K
Sbjct: 52 FWKWLRDQGVVSGKSV--AEPAVVPEGLGLVARRDIGRNEVVLEIPKRLWINPETVTASK 109
Query: 73 LDESYLGILLSKVEHWHGLILTILYEWKYLGEKSKWWPYFQVLPDQFDTLIYWDKEDLDK 132
+ G L ++ W + L ++ E KY E+S W Y +LP D+ ++W +E+L +
Sbjct: 110 I-----GPLCGGLKPWVSVALFLIRE-KY-EEESSWRVYLDMLPQSTDSTVFWSEEELAE 162
Query: 133 LKPSLVLD-RLG-EQQSKDMYVKVLELMKTFNVADKIGEVTYDDF 175
LK + +L LG ++ ++ ++K+ + + N +T DDF
Sbjct: 163 LKGTQLLSTTLGVKEYVENEFLKLEQEILLPNKDLFSSRITLDDF 207
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.136 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,966,967
Number of Sequences: 60738
Number of extensions: 1164038
Number of successful extensions: 3571
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 3556
Number of HSP's gapped (non-prelim): 11
length of query: 527
length of database: 30,389,216
effective HSP length: 111
effective length of query: 416
effective length of database: 23,647,298
effective search space: 9837275968
effective search space used: 9837275968
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)