ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI1936 good J KOG3429 Translation, ribosomal structure and biogenesis Predicted peptidyl-tRNA hydrolase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactI1936 660579 660085 -165 
         (165 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YOL114c [J] KOG3429 Predicted peptidyl-tRNA hydrolase                 147   6e-36
SPAC589.11 [J] KOG3429 Predicted peptidyl-tRNA hydrolase               53   2e-07
CE29441 [J] KOG3429 Predicted peptidyl-tRNA hydrolase                  52   5e-07
7297716 [J] KOG3429 Predicted peptidyl-tRNA hydrolase                  51   6e-07
Hs4557657 [J] KOG3429 Predicted peptidyl-tRNA hydrolase                50   1e-06

>YOL114c [J] KOG3429 Predicted peptidyl-tRNA hydrolase
          Length = 202

 Score =  147 bits (371), Expect = 6e-36
 Identities = 70/144 (48%), Positives = 90/144 (61%)

Query: 17  RAWIEGFKAENVPLKLFVATYDRSRGKGGQNVNKVNSKCTLTLYNFSKCSWFPDEIRKQL 76
           R W+       +PL  F+  YDR+ G GGQNVNKVNSKCTLTL   S C+W P E+R  L
Sbjct: 53  RNWVGALNVTGLPLNQFILRYDRASGPGGQNVNKVNSKCTLTLSGLSNCAWIPQEVRNIL 112

Query: 77  LDKGFRYYAPSKDALVIQSDETRSREQNRDICXXXXXXXXXXXXFFPGETDMSTKRKWNE 136
               FRYYA   D++VIQSDETRSRE N+  C             FP +T   T +KWN+
Sbjct: 113 SSGRFRYYAKGSDSIVIQSDETRSRETNKLKCFEKLVQEIRQTCQFPNDTTAETSKKWNK 172

Query: 137 IKKRSNEVRLSEKKFKSDKKRSRT 160
           IK+++N+ RL +KK  SDKK++R+
Sbjct: 173 IKEKANKERLLDKKVHSDKKKNRS 196


>SPAC589.11 [J] KOG3429 Predicted peptidyl-tRNA hydrolase
          Length = 182

 Score = 52.8 bits (125), Expect = 2e-07
 Identities = 38/131 (29%), Positives = 60/131 (45%), Gaps = 2/131 (1%)

Query: 36  TYDRSRGKGGQNVNKVNSKCTLTLYNFSKCSWFPDEIRKQLLDKGFRYYAPSKDALVIQS 95
           ++ RS G GGQNVNK+N+K  + L      S  P  +               ++ + I S
Sbjct: 52  SFSRSSGPGGQNVNKLNTKVIVNLPFKQLESCIPMFLINHFKTCEMLRNYRIQNGIKIYS 111

Query: 96  DETRSREQNRD--ICXXXXXXXXXXXXFFPGETDMSTKRKWNEIKKRSNEVRLSEKKFKS 153
            +TRS+ +N +  +              +  +T      + + +KK SNE RLSEKK+K 
Sbjct: 112 QKTRSQHKNIEDALNKISDLLNKSAETLYVPDTPPEKIARISILKKESNEKRLSEKKYKQ 171

Query: 154 DKKRSRTVKLD 164
            KK  R + +D
Sbjct: 172 KKKTQRRITMD 182


>CE29441 [J] KOG3429 Predicted peptidyl-tRNA hydrolase
          Length = 165

 Score = 51.6 bits (122), Expect = 5e-07
 Identities = 38/140 (27%), Positives = 62/140 (44%), Gaps = 4/140 (2%)

Query: 28  VPLKLFVATYDRSRGKGGQNVNKVNSKCTLTLYNFSKCSWFPDEIRKQLLDKGFRYYAPS 87
           +P +     Y  S G GGQNV K  +K  +  +  S+  W  + +R  L+++   +   +
Sbjct: 28  IPTEKIEKRYTLSSGPGGQNVQKNATKVEIR-FKVSEAEWLSESLR-DLVEEKLSHRINT 85

Query: 88  KDALVIQSDETRSREQNRDICXXXXXXXXXXXXFFPGETDMSTK--RKWNEIKKRSNEVR 145
              L+I SD TR R  N   C               G+ +M+ K  +   E    + + R
Sbjct: 86  AGELIIDSDRTRERHLNVADCFDKLRSAIYAIENEQGKREMTEKDEKILRERAAIATQHR 145

Query: 146 LSEKKFKSDKKRSRTVKLDF 165
           L EK+  S+KK SR   ++F
Sbjct: 146 LQEKRRTSEKKASRRAAVEF 165


>7297716 [J] KOG3429 Predicted peptidyl-tRNA hydrolase
          Length = 203

 Score = 51.2 bits (121), Expect = 6e-07
 Identities = 37/133 (27%), Positives = 58/133 (42%), Gaps = 3/133 (2%)

Query: 28  VPLKLFVATYDRSRGKGGQNVNKVNSKCTLTLYNFSKCSWFPDEIRKQLLDKGFRYYAPS 87
           +P+     TY RS G GGQ+VN VN+K  +  +  ++  W P++ R++LL K        
Sbjct: 65  IPMDRLEITYSRSSGPGGQHVNTVNTKVDVR-FKVAQADWIPEQTRQKLL-KVLANRITK 122

Query: 88  KDALVIQSDETRSREQN-RDICXXXXXXXXXXXXFFPGETDMSTKRKWNEIKKRSNEVRL 146
                I+SD TRS++ N  D                P      T  K    ++R+   RL
Sbjct: 123 DGYFYIKSDLTRSQQMNLADALEKLRTIIRSQEAVAPAPPSEETLEKLRRRQERAVRERL 182

Query: 147 SEKKFKSDKKRSR 159
             K+ ++  K  R
Sbjct: 183 QLKRGRAQVKADR 195


>Hs4557657 [J] KOG3429 Predicted peptidyl-tRNA hydrolase
          Length = 206

 Score = 50.4 bits (119), Expect = 1e-06
 Identities = 36/138 (26%), Positives = 60/138 (43%), Gaps = 2/138 (1%)

Query: 27  NVPLKLFVATYDRSRGKGGQNVNKVNSKCTLTLYNFSKCSWFPDEIRKQLLDKGFRYYAP 86
           ++PL     +Y RS G GGQNVNKVNSK  +  ++ +   W  + +R+++     +    
Sbjct: 71  DIPLDRLTISYCRSSGPGGQNVNKVNSKAEVR-FHLATAEWIAEPVRQKIAIT-HKNKIN 128

Query: 87  SKDALVIQSDETRSREQNRDICXXXXXXXXXXXXFFPGETDMSTKRKWNEIKKRSNEVRL 146
               L++ S+ +R + +N   C              P E      +      +  N  RL
Sbjct: 129 RLGELILTSESSRYQFRNLADCLQKIRDMITEASQTPKEPTKEDVKLHRIRIENMNRERL 188

Query: 147 SEKKFKSDKKRSRTVKLD 164
            +K+  S  K SR V +D
Sbjct: 189 RQKRIHSAVKTSRRVDMD 206


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.318    0.134    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,319,124
Number of Sequences: 60738
Number of extensions: 357619
Number of successful extensions: 1182
Number of sequences better than 1.0e-05: 5
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 1178
Number of HSP's gapped (non-prelim): 5
length of query: 165
length of database: 30,389,216
effective HSP length: 98
effective length of query: 67
effective length of database: 24,436,892
effective search space: 1637271764
effective search space used: 1637271764
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)