ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI1989 good R KOG2214 General function prediction only Predicted esterase of the alpha-beta hydrolase superfamily
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI1989 677067 679391 775
(775 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKR089c [R] KOG2214 Predicted esterase of the alpha-beta hydrola... 726 0.0
YOR081c [R] KOG2214 Predicted esterase of the alpha-beta hydrola... 597 e-170
SPAC1A6.05c [R] KOG2214 Predicted esterase of the alpha-beta hyd... 313 5e-85
At5g04040 [R] KOG2214 Predicted esterase of the alpha-beta hydro... 239 2e-62
At3g57140 [R] KOG2214 Predicted esterase of the alpha-beta hydro... 221 3e-57
SPCC1450.16c [R] KOG2214 Predicted esterase of the alpha-beta hy... 193 1e-48
SPAC31G5.20c [R] KOG2214 Predicted esterase of the alpha-beta hy... 158 3e-38
YMR313c [R] KOG2214 Predicted esterase of the alpha-beta hydrola... 112 3e-24
SPAC1786.01c [R] KOG2214 Predicted esterase of the alpha-beta hy... 80 2e-14
>YKR089c [R] KOG2214 Predicted esterase of the alpha-beta hydrolase
superfamily
Length = 910
Score = 726 bits (1874), Expect = 0.0
Identities = 396/722 (54%), Positives = 475/722 (64%), Gaps = 45/722 (6%)
Query: 31 NKGAEMTEEQSRNDSGQDEPQLNFFSSLCNSF-YKFIRGADSTHEQIARLIEQKQNARSL 89
N EM E D G+DE Q + +S C SF Y G I ++ QKQ+A S
Sbjct: 103 NNDIEMVE----TDEGEDERQGSSLASKCKSFLYNVFVGNYERDILIDKVCSQKQHAMSF 158
Query: 90 QEWLAAATKLDDITNAEQWKSKRESVLYDYCLVESSTEAMKKARLAKDWPNLLYLIRTTW 149
+EW +A +LDD+T +WK K ES LYDY L++ T M++ RL +++ LLY+IRT W
Sbjct: 159 EEWCSAGARLDDLTGKTEWKQKLESPLYDYKLIKDLTSRMREERLNRNYAQLLYIIRTNW 218
Query: 150 VRNRGGMGNVNLYRHSYVGTKHIIEDYVAESSRSLQELVYNSDLDDTYLLGMLTQTRKNI 209
VRN G MGNVNLYRHS+VGTK++I++Y+ ES +L+ L+ SDLDD+YLLG+L QTR+NI
Sbjct: 219 VRNLGNMGNVNLYRHSHVGTKYLIDEYMMESRLALESLM-ESDLDDSYLLGILQQTRRNI 277
Query: 210 GRTALVLSGGGTFGLFHIGILVTLFEQELLPRXXXXXXXXXXXXXXXXXRQEHELMGLMD 269
GRTALVLSGGGTFGLFHIG+L TLFE +LLPR + E+ L++
Sbjct: 278 GRTALVLSGGGTFGLFHIGVLGTLFELDLLPRVISGSSAGAIVASILSVHHKEEIPVLLN 337
Query: 270 EILVKDFNIFKDDLEMSESENLLIKISRFFKNGTWFDNKNLVSTMINFLGNLTFREAYNR 329
IL K+FNIFKDD + SESENLLIKISRFFKNGTWFDNK+LV+TMI FLG+LTFREAYNR
Sbjct: 338 HILDKEFNIFKDDKQKSESENLLIKISRFFKNGTWFDNKHLVNTMIEFLGDLTFREAYNR 397
Query: 330 SGKILNITVSPASVHEQPRLLNNLTSPNVLIWSAVCASCSLPGIFPSSPLYEKDPKTGET 389
+GKILNITVSPAS+ EQPRLLNNLT+PNVLIWSAVCASCSLPGIFPSSPLYEKDPKTGE
Sbjct: 398 TGKILNITVSPASLFEQPRLLNNLTAPNVLIWSAVCASCSLPGIFPSSPLYEKDPKTGER 457
Query: 390 KQWN-SSSVKFVDGSVDNDLPVSKLSEMFNVDHIIACQVNIHVFPFLKLSVSCVGGEIED 448
K W SSSVKFVDGSVDNDLP+S+LSEMFNVDHIIACQVNIHVFPFLKLS+SCVGGEIED
Sbjct: 458 KPWTGSSSVKFVDGSVDNDLPISRLSEMFNVDHIIACQVNIHVFPFLKLSLSCVGGEIED 517
Query: 449 EFSARFKMALGKVYKYMASEVIHALEIATEIGIGTNVLTKLRSVLSQQYSGDITILPEMN 508
EFSAR K L +Y +MA+E IH LEI +E+GI N LTKLRSVLSQQYSGDITILP+M
Sbjct: 518 EFSARLKQNLSSIYNFMANEAIHILEIGSEMGIAKNALTKLRSVLSQQYSGDITILPDMC 577
Query: 509 TLLRFNELLSNPTQEFLLREATNGVRASWPKISIIKNHCGQEFELDKMINYLKGKIISNT 568
L R ELLSNPT+EFLLRE TNG +A+WPK+SII+NHCGQEF LDK I+Y+KG++I +
Sbjct: 578 MLFRIKELLSNPTKEFLLREITNGAKATWPKVSIIQNHCGQEFALDKAISYIKGRMIVTS 637
Query: 569 DIKGNPFQISNRDVPLINSPVLANSEKKAIQ------------------VFXXXXXXXXX 610
+K PFQ ++ + LI +P + E K + V
Sbjct: 638 SLK-TPFQFADSVIGLIKAPEQTSDESKNPENSTLLTRTPTKGDNHISNVLDDNLLESES 696
Query: 611 XXXFMVVPDGTGSLKSQDLNNPPRYSNEFGSR--AHNRRKSDSATNGNRKGKRHNXXXXX 668
+++ + + PRYS S H RRKSD+ + R K
Sbjct: 697 TNSLLLLRENASTYGRSPSGFRPRYSITSASLNPRHQRRKSDTISTSRRPAK----SFSF 752
Query: 669 XXXXXXXXXXXXXXMINFRPS--FDKKTSTGDLQVRXXXXXXXXXXXXXRIDYDPRSNSL 726
IN P KKTS G L YDP S+ L
Sbjct: 753 SVASPTSRMLRQSSKINGHPPPILQKKTSMGRLMFPMDAKT-----------YDPESHEL 801
Query: 727 TP 728
P
Sbjct: 802 IP 803
>YOR081c [R] KOG2214 Predicted esterase of the alpha-beta hydrolase
superfamily
Length = 749
Score = 597 bits (1539), Expect = e-170
Identities = 301/567 (53%), Positives = 402/567 (70%), Gaps = 30/567 (5%)
Query: 17 PITSYVIKNYYKRANKGAEMTEEQSRNDSGQDEPQLNFFSSLCNSFYKFIRGADSTHEQ- 75
P+T +++ YY+ +N A DS +S +K++ + +Q
Sbjct: 6 PVTEFLLSKYYELSNTPA--------TDS--------------SSLFKWLYHKTLSRKQL 43
Query: 76 -IARLIEQKQNARSLQEWLAAATKLDDITNAEQWKSKRESVLYDYCLVESSTEAMKKARL 134
I+ L QK++A S +W A++LDD+T +WK+ ES LY+Y L++ T M+ R
Sbjct: 44 LISDLSSQKKHAISYDQWNDIASRLDDLTGLSEWKTIDESSLYNYKLLQDLTIRMRHLRT 103
Query: 135 AKDWPNLLYLIRTTWVRNRGGMGNVNLYRHSYVGTKHIIEDYVAESSRSLQELVYNSDLD 194
D+ LLYLIRT WVRN G M NVNLYRHS+ GTK II DY+ ES L L++ S+++
Sbjct: 104 THDYHRLLYLIRTKWVRNLGNMNNVNLYRHSHTGTKQIIHDYLEESQAVLTALIHQSNMN 163
Query: 195 DTYLLGMLTQTRKNIGRTALVLSGGGTFGLFHIGILVTLFEQELLPRXXXXXXXXXXXXX 254
D YLLG+L QTR+NIGRTALVLSGG TFGLFHIG+L LFE +L+P+
Sbjct: 164 DHYLLGILQQTRRNIGRTALVLSGGSTFGLFHIGVLAALFESDLMPKVISGSSAGAIVAS 223
Query: 255 XXXXRQEHELMGLMDEILVKDFNIFKDDLEMSESENLLIKISRFFKNGTWFDNKNLVSTM 314
E+ L+ +L +FNIF DD S +ENLLIKISRF +NGTWF+N+ L++TM
Sbjct: 224 IFCVHTTQEIPSLLTNVLNMEFNIFNDDNSKSPNENLLIKISRFCQNGTWFNNQPLINTM 283
Query: 315 INFLGNLTFREAYNRSGKILNITVSPASVHEQPRLLNNLTSPNVLIWSAVCASCSLPGIF 374
++FLGNLTFREAYN++GKILNITVSPAS++EQP+LLNNLT+PNVLIWSAVCASCSLPG+F
Sbjct: 284 LSFLGNLTFREAYNKTGKILNITVSPASIYEQPKLLNNLTAPNVLIWSAVCASCSLPGVF 343
Query: 375 PSSPLYEKDPKTGETKQWNS-----SSVKFVDGSVDNDLPVSKLSEMFNVDHIIACQVNI 429
PS+PL+EKDP TG+ K+W + S++KF+DGSVDND+P+S+LSEMFNVDHIIACQVNI
Sbjct: 344 PSTPLFEKDPHTGKIKEWGATNLHLSNMKFMDGSVDNDMPISRLSEMFNVDHIIACQVNI 403
Query: 430 HVFPFLKLSVSCVGGEIEDEFSARFKMALGKVYKYMASEVIHALEIATEIGIGTNVLTKL 489
HVFP LK S +CVGGEIE E +ARF+ + K++K+ + E IH L+I E+ ++TKL
Sbjct: 404 HVFPLLKFSNTCVGGEIEKEITARFRNQVTKIFKFFSDETIHFLDILKELEFHPYLMTKL 463
Query: 490 RSVLSQQYSGDITILPEMNTLLRFNELLSNPTQEFLLREATNGVRASWPKISIIKNHCGQ 549
+ + QQYSG++TILP+++ + +F+E+L NP+Q FLL + T G RA+WPKIS+I+N+CGQ
Sbjct: 464 KHLFLQQYSGNVTILPDLSMVGQFHEVLKNPSQLFLLHQTTLGARATWPKISMIQNNCGQ 523
Query: 550 EFELDKMINYLKGKIISNTDIKGNPFQ 576
EF LDK I +LK KII ++ IK NP Q
Sbjct: 524 EFALDKAITFLKEKIIISSSIK-NPLQ 549
>SPAC1A6.05c [R] KOG2214 Predicted esterase of the alpha-beta hydrolase
superfamily
Length = 483
Score = 313 bits (803), Expect = 5e-85
Identities = 179/471 (38%), Positives = 269/471 (57%), Gaps = 16/471 (3%)
Query: 82 QKQNARSLQEWLAAATKLDDITNAEQWKSKRESVLYDYCLVESSTEAMKKARLAKDWPNL 141
Q + A S + WL AA KLD I QW+ ++ES YDY LVES +++ RL+K+ L
Sbjct: 9 QMEYASSYETWLEAAEKLDVIEGKYQWREQKESDEYDYVLVESRLHELRRHRLSKNTRLL 68
Query: 142 LYLIRTTWVRNRGGMGNVNLYRHSYVGTKHIIEDYVAESSRSLQELVYNSDLDDTYLLGM 201
L L+R + R+ M N LY +++ GTK +I++++ E L L DL +
Sbjct: 69 LGLLRNSVARDFANMDNSRLYNYAHSGTKKLIDEFIQEVLMCLTYLEETPDLSLDEKITE 128
Query: 202 LTQTRKNIGRTALVLSGGGTFGLFHIGILVTLFEQELLPRXXXXXXXXXXXXXXXXXRQE 261
++ + G TAL+LSGGGTFG+ HIG+L +L EQ L+P+ R +
Sbjct: 129 FSRLKLTTGNTALILSGGGTFGMTHIGVLQSLHEQGLVPKIICGSSAGAIVACAAAVRNK 188
Query: 262 HELMGLMDEILVKDFNIFKDDLEMSESENLLIKISRFFKNGTWFDNKNLVSTMINFLGNL 321
E L+ + D ++F D + +++ + ++F G D +L M +G+
Sbjct: 189 EEQEILLRQFHTGDLSVFTD--PNAAPPSVIQSVKQYFTRGCVLDISHLERVMKLLIGDF 246
Query: 322 TFREAYNRSGKILNITVSPASVHEQPRLLNNLTSPNVLIWSAVCASCSLPGIFPSSPLYE 381
TF+EAY+RSG ILN+TVS S+ E P LLN +T+PNVL+WSAV A+CS+P +F + L+E
Sbjct: 247 TFQEAYDRSGYILNVTVSCGSLFEMPSLLNYITAPNVLVWSAVVATCSVPFLFKRATLWE 306
Query: 382 KDPKTGETKQW-NSSSVKFVDGSVDNDLPVSKLSEMFNVDHIIACQVNIHVFPFLKLSVS 440
+DP T E + + + ++DGSVDND+P +KL+E+F+V+H I QVN H+ PF
Sbjct: 307 RDPLTREVSAFCVTDAPLWMDGSVDNDIPHAKLTELFHVNHFIVSQVNFHIVPF------ 360
Query: 441 CVGGEIEDEFSARF-KMALGKVYKYMASEVIHALEIATEIGIGTNVLTKLRSVLSQQYSG 499
I D S + + K A EV + E+GI + + TKL+SV++Q+YSG
Sbjct: 361 -----IMDPTSHNWVERCCKKAIDLAAQEVSLTFRLFAELGIFSVLFTKLQSVITQKYSG 415
Query: 500 DITILPEMNTLLRFNELLSNPTQEFLLREATNGVRASWPKISIIKNHCGQE 550
DITI+P +N N+++ NPT FLL AT G R +W K+ + +NHC E
Sbjct: 416 DITIIPRLN-YREVNKVIKNPTPSFLLDAATRGKRGTWTKVPVTRNHCAIE 465
>At5g04040 [R] KOG2214 Predicted esterase of the alpha-beta hydrolase
superfamily
Length = 825
Score = 239 bits (609), Expect = 2e-62
Identities = 165/495 (33%), Positives = 248/495 (49%), Gaps = 48/495 (9%)
Query: 84 QNARSLQEWLAAATKLDDITNAEQWKSKRESVLYDYCLVESSTEAMKKARLAKDWPNLLY 143
+ A + +EW AA L+ T ES LYD LV++ + ++ R ++++
Sbjct: 106 RTALTYEEWAHAAKMLEKET-----PKMNESDLYDEELVKNKLQELRHRRQEGSLRDIMF 160
Query: 144 LIRTTWVRNRGGMGNVNLYRHSYVGTKHIIEDYVAESSRSLQELVYNSDLDDTYL---LG 200
+R VRN G M N L++ +HI ++Y+ E S L+ +V NSD ++ L L
Sbjct: 161 CMRADLVRNLGNMCNSELHKGRLQVPRHI-KEYIDEVSTQLR-MVCNSDSEELSLEEKLS 218
Query: 201 MLTQTRKNIGRTALVLSGGGTFGLFHIGILVTLFEQELLPRXXXXXXXXXXXXXXXXXRQ 260
+ +TR GRTAL+LSGG + G FH+G++ TL E +LLPR R
Sbjct: 219 FMHETRHAFGRTALLLSGGASLGAFHVGVVRTLVEHKLLPRIIAGSSVGSIICAVVASRS 278
Query: 261 EHELMGLMDEILVKDFNIFKDDLEMSESENLLIKISRFFKNGTWFDNKNLVSTMINFLGN 320
EL + L F + + + R G D + L + N N
Sbjct: 279 WPELQSFFENSL-HSLQFF------DQLGGVFSIVKRVMTQGALHDIRQLQCMLRNLTSN 331
Query: 321 LTFREAYNRSGKILNITVSPASVHEQPRLLNNLTSPNVLIWSAVCASCSLPGIF------ 374
LTF+EAY+ +G+IL ITV HE PR LN LTSP+V+IWSAV ASC+ PG+F
Sbjct: 332 LTFQEAYDMTGRILGITVCSPRKHEPPRCLNYLTSPHVVIWSAVTASCAFPGLFEAQELM 391
Query: 375 ---------PSSPLYEKDPKTGETKQWNSSSVKFVDGSVDNDLPVSKLSEMFNVDHIIAC 425
P P + DP+ G TK +SS ++ DGS++ DLP+ +L E+FNV+H I
Sbjct: 392 AKDRSGEIVPYHPPFNLDPEVG-TK--SSSGRRWRDGSLEVDLPMMQLKELFNVNHFIVS 448
Query: 426 QVNIHVFPFLKLSVSCVGGEIEDEFSARFKMALGKVYKYMASEVIHALEIATEIGIGTNV 485
Q N H+ P L+L ++ + RF K+ + EV H E+G
Sbjct: 449 QANPHIAPLLRLK------DLVRAYGGRF---AAKLAHLVEMEVKHRCNQVLELGFPLGG 499
Query: 486 LTKLRSVLSQQYSGDITILPEMNTLLRFNELLSNPTQEFLLREATNGVRASWPKISIIKN 545
L KL +Q++ GD+T++ TL ++++++ NPT L + A G R +W K+S IK+
Sbjct: 500 LAKL---FAQEWEGDVTVVMPA-TLAQYSKIIQNPTHVELQKAANQGRRCTWEKLSAIKS 555
Query: 546 HCGQEFELDKMINYL 560
+CG E LD + L
Sbjct: 556 NCGIELALDDSVAIL 570
>At3g57140 [R] KOG2214 Predicted esterase of the alpha-beta hydrolase
superfamily
Length = 801
Score = 221 bits (564), Expect = 3e-57
Identities = 156/491 (31%), Positives = 252/491 (50%), Gaps = 46/491 (9%)
Query: 86 ARSLQEWLAAATKLDDITNAEQWKSKRESVLYDYCLVESSTEAMKKARLAKDWPNLLYLI 145
A + +EW AA LD T E+ L+D LV + + +K R ++++ +
Sbjct: 109 ALTYEEWSHAAKMLDKET-----PKVNETDLFDVELVSNKLDELKHRRHEGSLRDIIFCM 163
Query: 146 RTTWVRNRGGMGNVNLYRHSYVGTKHIIEDYVAESSRSLQELVYNSDLDDTYL---LGML 202
R VRN G M N L++ + +I++Y+ E S L+ +V + D ++ L L +
Sbjct: 164 RADLVRNLGNMCNPELHK-GRLHVPRLIKEYIDEVSTQLR-MVCDMDTEELSLEEKLSFM 221
Query: 203 TQTRKNIGRTALVLSGGGTFGLFHIGILVTLFEQELLPRXXXXXXXXXXXXXXXXXRQEH 262
+TR GRTAL+LSGG + G FH+G++ TL E +LLPR R
Sbjct: 222 HETRHAYGRTALLLSGGASLGAFHLGVVKTLVEHKLLPRIIAGSSVGSVMCAVVGTRSWP 281
Query: 263 ELMGLMDEILVKDFNIFKDDLEMSESENLLIKISRFFKNGTWFDNKNLVSTMINFLGNLT 322
EL + ++ + +M + + R G + ++L + N NLT
Sbjct: 282 ELQSFFEG----SWHALQFFDQMG---GIFTTVKRVMTQGAVHEIRHLQWKLRNLTNNLT 334
Query: 323 FREAYNRSGKILNITVSPASVHEQPRLLNNLTSPNVLIWSAVCASCSLPGIFPSSPLYEK 382
F+EAY+ +G+IL ITV HE PR LN LTSP+V+IWSAV ASC+ PG+F + L K
Sbjct: 335 FQEAYDITGRILGITVCSLRKHEPPRCLNYLTSPHVVIWSAVTASCAFPGLFEAQELMAK 394
Query: 383 DPKTGE-----------TKQWNSSSVKFVDGSVDNDLPVSKLSEMFNVDHIIACQVNIHV 431
D +TGE ++ ++S ++ DGS++ DLP+ +L E+FNV+H I Q N H+
Sbjct: 395 D-RTGEIVPYHPPFNLDPEEGSASVRRWRDGSLEMDLPMIQLKELFNVNHFIVSQANPHI 453
Query: 432 FPFLKLS--VSCVGGEIEDEFSARFKMALGKVYKYMASEVIHALEIATEIGIGTNVLTKL 489
PFL++ V GG F+A K+ + EV H E+G+ L ++
Sbjct: 454 APFLRMKEFVRACGG----RFAA-------KLAQLAEMEVKHRCNQVLELGL---PLREV 499
Query: 490 RSVLSQQYSGDITILPEMNTLLRFNELLSNPTQEFLLREATNGVRASWPKISIIKNHCGQ 549
S+ +Q++ GD+TI+ T ++ +++ NP+ + + A G R +W K+++IK + G
Sbjct: 500 ASLFAQEWEGDVTIVMPA-TFSQYLKIIQNPSNVEIQKAANQGRRCTWEKLAVIKANFGI 558
Query: 550 EFELDKMINYL 560
E LD+ + L
Sbjct: 559 ELALDECVTVL 569
>SPCC1450.16c [R] KOG2214 Predicted esterase of the alpha-beta hydrolase
superfamily
Length = 513
Score = 193 bits (490), Expect = 1e-48
Identities = 138/503 (27%), Positives = 236/503 (46%), Gaps = 33/503 (6%)
Query: 61 SFYKFIRGADSTHEQIAR-----LIEQKQNARSLQEWLAAATKLDDITNAEQWKSKRESV 115
SF++++ A S +Q + ++ RS ++W AT +D + +W+ S
Sbjct: 24 SFFEWLFSATSISQQRGSGPRKGKVVMNKDCRSWEDWKVLATTIDKASGRWKWRFTPASD 83
Query: 116 LYDYCLVESSTEAMKKARLAKDWPNLLYLIRTTWVRNRGGMGNVNLYRHSYVGTKHIIED 175
YDY L++ T ++K+ R K +L +R++ +RN G +GN +LY +Y GTK +IE+
Sbjct: 84 KYDYLLIDRCTVSLKRYRQRKSVYPMLMFLRSSLLRNFGNIGNSSLYTENYSGTKILIEE 143
Query: 176 YVAESSRSLQELVYNSDLDDTYLLGMLTQTRKNIGRTALVLSGGGTFGLFHIGILVTLFE 235
YV E + L+ L + L + R + G T L +GG FGL+H G+ TL++
Sbjct: 144 YVREVNNCLEFLYHTKRLSYDVKCDFFSAARISFGTTCLYFNGGTAFGLYHFGVAKTLWK 203
Query: 236 QELLPRXXXXXXXXXXXXXXXXXRQEHELMGLMDEILVKDFNIFKDDLEMSESENLLIKI 295
+ LLP+ ++ EL GL D + + I + + S +
Sbjct: 204 RNLLPQILAGCASGALIASLLSVYRDEELNGLFDTFPSELWKICQQTSDYS--------L 255
Query: 296 SRFFKNGTWFDNKNLVSTMINFLGNLTFREAYNRSGKILNITVSPASVHEQPRLLNNLTS 355
S+ + G D + S + LG +TF+EA+ R+G+I+NI P++V P++LN T+
Sbjct: 256 SKVVEYGNMLDISMIASFVRQRLGTITFQEAFERTGRIVNIVAPPSAVSGSPQVLNYFTA 315
Query: 356 PNVLIWSAVCASCSLPGIFPSSPLYEKDPKTGETKQWNSSSVKFVDGSVDN---DLPVSK 412
PNVLIWSAVC+S S I+ SSPL K P G T+ + + + N P ++
Sbjct: 316 PNVLIWSAVCSSNSWAAIYRSSPLLAKLP-DGSTEVCTPKNFIWPYAGLPNTGRSNPYAR 374
Query: 413 LSEMFNVDHIIACQVNIHVFPFLKLSVSCVGGEIEDEFSARFKMALGKVYKYMASEVIHA 472
+SE+FNV+H + Q +FP E+ + + + K+ + + E+ +
Sbjct: 375 ISEIFNVNHFVITQSRPSLFPTFY-------DELHHHRVSGYSL---KMIRLVGLEMAYR 424
Query: 473 LEIATEIGIGTNVLTKLRSVLSQQY--SGDITILPEMNTLLRFNELLSNPTQEFLLREAT 530
+G+ + +LR Y S IT+ P + + P+ +
Sbjct: 425 FRQLDILGL---LPPRLRRFFVDDYVPSAYITLTPTF-SFSDIKHAFTKPSLSDIQYWIL 480
Query: 531 NGVRASWPKISIIKNHCGQEFEL 553
G RA+W I +++ C E L
Sbjct: 481 VGERATWQAIPLLQVRCKTEISL 503
>SPAC31G5.20c [R] KOG2214 Predicted esterase of the alpha-beta hydrolase
superfamily
Length = 405
Score = 158 bits (400), Expect = 3e-38
Identities = 112/340 (32%), Positives = 163/340 (47%), Gaps = 15/340 (4%)
Query: 208 NIGRTALVLSGGGTFGLFHIGILVTLFEQELLPRXXXXXXXXXXXXXXXXXRQEHELMGL 267
N GRTAL LSGG +F ++H G+L L Q+L+P R EL L
Sbjct: 20 NYGRTALCLSGGASFAIYHTGVLRALLNQDLIPNVITGTSGGGLLAALVCTRTNEELKQL 79
Query: 268 MDEILVKDFNIFKDDLEMSESENLLIKISRFFKNGTWFDNKNLVSTMINFL-GNLTFREA 326
+ L + S+ N L R+F+ G FD T + F G+LTF EA
Sbjct: 80 LVPELASKYQ--------SDIGNWLDATKRYFRTGARFDEILWAKTCMYFTRGSLTFAEA 131
Query: 327 YNRSGKILNITVSPASVHEQPRLLNNLTSPNVLIWSAVCASCSLPGIFPSSPLYEKDPKT 386
Y R+G+ILNI+V P+ VH P+L+N LTSP+ +IWSAV ASC++PGI PL +
Sbjct: 132 YKRTGRILNISVIPSDVHSPPKLINYLTSPDTVIWSAVIASCAVPGILNPIPLMTRSQSH 191
Query: 387 GETKQWNSSSVKFVDGSVDNDLPVSKLSEMFNVDHIIACQVNIHVFPFLKLSVSCVGGEI 446
++ +F DGS+ D+P+S+L FNV I Q N HV F VG +
Sbjct: 192 RLIP--HNFGNRFKDGSLRTDIPLSELRTQFNVHFSIVSQTNPHVQVFFFSPRGTVGRPV 249
Query: 447 EDEFSARFKMAL--GKVYKYMASEVIHALEIATEIGIGTNVL-TKLRSVLSQQYSGDITI 503
++ + +++ ++I L + + + L T SV Q++ G ITI
Sbjct: 250 SHRKGRGWRGGYVGSAIEQFLKYDMIKWLHVIRSLELLPRPLGTDWSSVFLQKFDGTITI 309
Query: 504 LPEMNTLLRFNELLSNPTQEFLLREATNGVRASWPKISII 543
P+ F +LS P+ E L G A++PK+ I
Sbjct: 310 WPK-TKFQDFYYILSPPSVERLGYMIDAGQAATFPKLDFI 348
>YMR313c [R] KOG2214 Predicted esterase of the alpha-beta hydrolase
superfamily
Length = 642
Score = 112 bits (279), Expect = 3e-24
Identities = 100/379 (26%), Positives = 168/379 (43%), Gaps = 32/379 (8%)
Query: 80 IEQKQNARSLQEWLAAATKLDDITNAEQWKSKRESVLYDYCLVESSTEAMKKARLAKDWP 139
I++ + Q W A+ +D+IT A W+ S YD+ V ++ + +
Sbjct: 71 IKKLDECDTYQMWCQQASVVDEITGANLWRRNFFSRRYDFNSVIEQYSILENMLREEKYD 130
Query: 140 NLLYLIRTT---WVRNRGGMGNVNLYRHSYVGTKHIIEDYVAESSRSLQELVYNSDLDDT 196
+ TT +RN G+G+ L+ S +GTK +IE Y+ L +++ N L T
Sbjct: 131 VVKEKFSTTGPCMLRNFAGIGDKKLFTKSLMGTKLLIEQYLTRILEGL-DILNNQTLTPT 189
Query: 197 YLLGMLTQTRKNIGRTALVLSGGGTFGLFHIGILVTLFEQELLPRXXXXXXXXXXXXXXX 256
+ + ++G TAL+L GG FGLFH+G++ L Q+L+P
Sbjct: 190 ---SFFQRCKLSLGTTALILQGGSLFGLFHLGVIRGLLLQDLMPNIISGSSMGACVASLF 246
Query: 257 XXRQEHELMGLM--DEILVKDFNIFKDDLEMSES---------ENLLIKISRFFKNGTWF 305
+L L+ D +L NI K+D+++ +S NL I +G
Sbjct: 247 GCLSNEQLKQLLTDDNLL----NIIKNDVDLLKSCGYGNLEQHLNLGTLIQNLIHHGYSQ 302
Query: 306 DNKNLVSTMINFL-GNLTFREAYNRSGKILNITVSPASVHEQPRLLNNLTSPNVLIWSAV 364
D + ++ ++ TF E Y +GK+ NI + P P LLN +T+PNVLI SA+
Sbjct: 303 DVYLFIRFVMKYIVKEKTFEEVYQITGKVFNIVIHPTD-KSCPNLLNYVTTPNVLIKSAI 361
Query: 365 CASCSLPGIFPSSPLYEKDPKTGETKQWN---SSSVKFVDGSVDN-----DLPVSKLSEM 416
S I + L K+ + N + VKF+ N + P ++L+E+
Sbjct: 362 ECSLGSGVISEDTSLLCKNLENEIEPFLNINKNKQVKFLTPENANNPSITESPYTRLTEL 421
Query: 417 FNVDHIIACQVNIHVFPFL 435
FNV++ I ++ P +
Sbjct: 422 FNVNNFIVSLARPYLAPLV 440
>SPAC1786.01c [R] KOG2214 Predicted esterase of the alpha-beta hydrolase
superfamily
Length = 260
Score = 79.7 bits (195), Expect = 2e-14
Identities = 56/182 (30%), Positives = 82/182 (44%), Gaps = 11/182 (6%)
Query: 52 LNFFSSLCNSFYKFIRGADSTH-----------EQIARLIEQKQNARSLQEWLAAATKLD 100
L F + LC +F F+ D + + RL + Q A++ +EW A LD
Sbjct: 78 LMFTAFLCLTFVAFLYLLDRLYINCYEYFIVWRGEARRLRKLLQEAKTYEEWKERARALD 137
Query: 101 DITNAEQWKSKRESVLYDYCLVESSTEAMKKARLAKDWPNLLYLIRTTWVRNRGGMGNVN 160
++WK YDY LV++ ++ K R KDW L ++ N GG+ +
Sbjct: 138 KYFGNDEWKLDPVYDYYDYTLVQAVYSSLVKHREQKDWNALKSVLDVCVRSNFGGIDSSM 197
Query: 161 LYRHSYVGTKHIIEDYVAESSRSLQELVYNSDLDDTYLLGMLTQTRKNIGRTALVLSGGG 220
LY +Y GTK ++EDYV E L+ ++ M N GRTAL LSGG
Sbjct: 198 LYSRTYSGTKKLVEDYVNELKVCLETVIDQRLYTAQERSKMFEYFSHNYGRTALCLSGGA 257
Query: 221 TF 222
+F
Sbjct: 258 SF 259
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.133 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,331,475
Number of Sequences: 60738
Number of extensions: 1787382
Number of successful extensions: 4660
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 4619
Number of HSP's gapped (non-prelim): 14
length of query: 775
length of database: 30,389,216
effective HSP length: 114
effective length of query: 661
effective length of database: 23,465,084
effective search space: 15510420524
effective search space used: 15510420524
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)