ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI2073 good YU KOG4121 Intracellular trafficking, secretion, and vesicular transport Nuclear pore complex, Nup133 component (sc Nup133)
r_klactI2073 good YU KOG4121 Nuclear structure Nuclear pore complex, Nup133 component (sc Nup133)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI2073 705853 702536 -1106
(1106 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKR082w [YU] KOG4121 Nuclear pore complex Nup133 component (sc N... 553 e-157
SPAC1805.04 [YU] KOG4121 Nuclear pore complex Nup133 component (... 135 5e-31
SPBP35G2.06c [YU] KOG4121 Nuclear pore complex Nup133 component ... 73 3e-12
>YKR082w [YU] KOG4121 Nuclear pore complex Nup133 component (sc Nup133)
Length = 1157
Score = 553 bits (1425), Expect = e-157
Identities = 368/1156 (31%), Positives = 594/1156 (50%), Gaps = 69/1156 (5%)
Query: 6 QLRKELSVPAAAPED---SVISAEETTTGSAVT----------NVQFSNEQVITENGKYK 52
+LRKELSVP A E+ + +S EE + S + + F N +V TEN +Y
Sbjct: 9 RLRKELSVPIAVVENESLAQLSYEEESQASLMDISMEQQQLRLHSHFDNSKVFTENNRYI 68
Query: 53 VSKLAPSLSFLPSNV-ELCCSVDAKSGYALCCDTDNVYVWPFLSSETHPSFVRIPLHDEE 111
V L S SN EL +D + GY L D VY+W S++ ++ +P ++
Sbjct: 69 VKTLQTDYSSGFSNDDELNGYIDMQIGYGLVNDHKKVYIWNIHSTQKDTPYITVPFRSDD 128
Query: 112 YESWSRPPLCCFTWPSVLER--------DLKSSPGLFLVNSRSGDIQFYEDVDTVSQVAS 163
+ + P C T+P+ ++ D + GL ++ + +YED+++++ +
Sbjct: 129 NDEIAVAPRCILTFPATMDESPLALNPNDQDETGGLIII--KGSKAIYYEDINSINNLNF 186
Query: 164 FLTQTKRHKSVLKLRD--GEYVENLINAEPAGILLSTNYGRVEFCTIRDANGKPFVSLKQ 221
L++ H+ L + GE + ++N EPAGI+LSTN GR+ F TIR++ GKP + L +
Sbjct: 187 KLSEKFSHELELPINSSGGEKCDLMLNCEPAGIVLSTNMGRIFFITIRNSMGKPQLKLGK 246
Query: 222 TIIK-CQVGIFSSM-SKSKRVVSLKLGEISGKGERIVSILTQGGRYQVWNLSSVGNCYKK 279
+ K ++GI+S + + + VVSL+ G I GKG R+V I T G +Q W LS+ + K
Sbjct: 247 LLNKPFKLGIWSKIFNTNSSVVSLRNGPILGKGTRLVYITTNKGIFQTWQLSATNSHPTK 306
Query: 280 -IDLNVQSEISQAVLEHFPLATSTLKLLDCHPLSGDSDAYLFLSFLAES--NETHYLLST 336
ID+N+ I +++ + +P A TLK+ D HPL D + LFLS + +S NET+Y+LST
Sbjct: 307 LIDVNIYEAILESLQDLYPFAHGTLKIWDSHPLQ-DESSQLFLSSIYDSSCNETYYILST 365
Query: 337 IIVDEDTNSFAIFSTYRLNTYTRPLSD-EFAPRLIVPTAYTKEHL-PITSVYTLFSDAAV 394
II D +NSF IFSTYRLNT+ ++D +F P++ +P +TS+ +F +A V
Sbjct: 366 IIFDSSSNSFTIFSTYRLNTFMESITDTKFKPKIFIPQMENANDTNEVTSILVMFPNAVV 425
Query: 395 LTQTSSKLDSNYPFKRKWEDIIRFNKDMKVLGYDCNSSSLYITDGRTCALKLETFEKYEN 454
+TQ +SKLDS+Y +RKWEDI+ D+ ++G +S SLY+ + L+ E E
Sbjct: 426 ITQVNSKLDSSYSMRRKWEDIVSLRNDIDIIGSGYDSKSLYVLTKQMGVLQFFVKENEET 485
Query: 455 SDFQEIRFIKSHVDQYVYFGGQSTXXXXXXXXXXXXXXQPQEIETDIQMSSDEIKYALSN 514
+ E+ F+KSHVDQ VYF + Q + IE D++++S+EI ++
Sbjct: 486 NSKPEVGFVKSHVDQAVYFSKINANPIDFNLPPEISLDQ-ESIEHDLKLTSEEIFHSNGK 544
Query: 515 HIPPVANNPMKHLKIRSELFERLLTFVAHNFLDQTSSQFKLDLISDFEMIKCLESLYSLL 574
+IPP+ N +HL +R E F+ LTFVA NF + S + KLDLI FE++ C S++
Sbjct: 545 YIPPMLNTLGQHLSVRKEFFQNFLTFVAKNFNYKISPELKLDLIEKFEILNCCIKFNSII 604
Query: 575 PQQPTSISQAWKTVLSESSYQSDSDFISDGIV-----KIPELLGAFLKHLKDLMKSTNET 629
+Q ++ W+ LS + + + +V P + FL H+ ++ +
Sbjct: 605 -RQSDVLNDIWEKTLSNYNLTQNEHLTTKTVVINSPDVFPVIFKQFLNHVVFVLFPSQNQ 663
Query: 630 MLKSQIAKLINQLVYHSILEECENSYRYGLFQMSPDDV-SKNIPWFTTNSIIENLNDIFF 688
K + LIN Y ILEE E + RY L ++ P +V + +PWF + +N FF
Sbjct: 664 NFKLNVTNLINLCFYDGILEEGEKTIRYELLELDPMEVDTSKLPWFINFDYLNCINQCFF 723
Query: 689 LLRHASEDXXXXXXXXXXXXXXXXXXXYMVRQITMWIDANAITD-------SSYKQFFEE 741
A E+ Y Q +WI+ + + +++
Sbjct: 724 DFTFACEEEGSLDSYKEGLLKIVKILYYQFNQFKIWINTQPVKSVNANDNFININNLYDD 783
Query: 742 NHLLWNRVLRDVNRSQDSILITDFYQDFEGLVETLQSLPK--ITAFSLYDEFFQKYGYDF 799
NHL WN VL VN + I I +FY+D GLV+TLQ+L + T SLY+ FF ++ +F
Sbjct: 784 NHLDWNHVLCKVNLKEQCIQIAEFYKDLSGLVQTLQTLDQNDSTTVSLYETFFNEFPKEF 843
Query: 800 AKSLFSYYIKEGLWDDLYESFGETHSEYLDQFLGECPEYGKVKWIGNIYCHKYDEATTTL 859
+ +LF Y IK +DL F + H + F P+YG V WI I Y +A TL
Sbjct: 844 SFTLFEYLIKHKKLNDLIFRFPQQHDVLIQFFQESAPKYGHVAWIQQILDGSYADAMNTL 903
Query: 860 LSISTGTQSLGQDLKQRQAQLSIAKLTALAAEVQ--DLEKLNEIQTELDLIDGQILLSQE 917
+I+ G+ L + + L++AKL++L E D+ L +IQ LD ID + +S +
Sbjct: 904 KNITVDDSKKGESLSECELHLNVAKLSSLLVEKDNLDINTLRKIQYNLDTIDAEKNISNK 963
Query: 918 LQESTLNPLFKDFT--TYRTLFKYLSDAVKRRESLSVPHMIELFTLLNQDTSYGFALSIL 975
L++ + + K F + R +F L + +K +++ ++EL+++L+ + S L +L
Sbjct: 964 LKKGEVQ-ICKRFKNGSIREVFNILVEELKSTTVVNLSDLVELYSMLDDEESLFIPLRLL 1022
Query: 976 AID-HTLPTEVKKYCEYQIWRRCLL------SDENAVRQTLRVFFKQQFYHQSIELPIID 1028
++D + L EVKK+ +WRR +L D+ RVF ++ + LP +D
Sbjct: 1023 SVDGNLLNFEVKKFLNALVWRRIVLLNASNEGDKLLQHIVKRVFDEELPKNNDFPLPSVD 1082
Query: 1029 NLANEKLITDSYLDGMYHGL-TDLNKLASDVKREVALFANSNLDVTM---LQSIIGEINE 1084
L ++ L+T Y+ Y D N + ++ E++ N D ++ L S IG + +
Sbjct: 1083 LLCDKSLLTPEYISETYGRFPIDQNAIREEIYEEISQVETLNSDNSLEIKLHSTIGSVAK 1142
Query: 1085 ETGNQCVINYATNTIE 1100
E INY TNT+E
Sbjct: 1143 E--KNYTINYETNTVE 1156
>SPAC1805.04 [YU] KOG4121 Nuclear pore complex Nup133 component (sc Nup133)
Length = 1162
Score = 135 bits (339), Expect = 5e-31
Identities = 228/1150 (19%), Positives = 464/1150 (39%), Gaps = 169/1150 (14%)
Query: 1 MKQLFQLRKELSVPAAAPEDSVISAEETTTGSAVTNVQFSNEQVITENGKYKVSKLAPSL 60
M + RK V + +P IS E+ S + + ++N + +KVS L
Sbjct: 1 MSSILGKRKNEEVVSFSPLKR-ISVEK----SLLDSTAYNNSLDWLRSKNFKVSCLLKHF 55
Query: 61 -SFLPSNVELCCSVDAKSGYALCCDTDNVYVWPFLSS--ETHPSFVRIPLHDEEYESWS- 116
S + ++ L S GYAL ++ + S P + PL +E+ +S
Sbjct: 56 NSKIINDHPLSGSCYTDIGYALINSRKACFILSYRQSLGTAEPPTITFPLPEEDSNGFSG 115
Query: 117 RPPLCCFTWPSVLERDLKSSPGLFLVNSRSGDIQFYEDVDTVSQVASFLTQTKRHKSVLK 176
+ L F +++ PGL +V SG I ++ + + Q +S++K
Sbjct: 116 QNALTAFVPSDASDKE----PGLLIVMPISGRIAYWTSIGNALAQSYICPQGM--ESLIK 169
Query: 177 LRDGEYVENLINAEPAGILLSTNYGRVEFCTIRDANGKPFVSLKQTIIKCQVGIFSSMSK 236
L E E+L + P ++STN+GR+ +RD G+P VS++ + + FS++ +
Sbjct: 170 LLPKEKCEHLCCSNPMKFIISTNFGRLFSVQLRDPAGQPDVSVQ--LFASDISTFSTILQ 227
Query: 237 --------SKRVVSLKLGEISGKGERIVSILTQGGRYQVWNLSSVGNCYKKIDLNVQSEI 288
S +++LK + + ++ + G ++++L ++L S I
Sbjct: 228 KMKIFNYPSIHIIALKSPPLFSPYQHLLYVAEASGLLEIYDLKLENKLVSGMNL---SPI 284
Query: 289 SQAVLEHFPLATSTLKLLDCH--PLSGDSDAYLFLSFLAESNETHYLLSTIIVD-EDTNS 345
+ VL S L++LD P +G+ ++L + N +Y I +D D +S
Sbjct: 285 FKQVLREGCPDASGLEVLDLTICPTNGNLVSFL----VCWKNSINYRYMIISLDFSDISS 340
Query: 346 FAIFSTYRLNTYTRPLSDEFAPRLIVPTAYTKEHLPIT--SVYTLFSDAAVLTQTSSKLD 403
++ + + L +++ + +K H + S++ + +DA ++ D
Sbjct: 341 PSVMNIHPLYSFSSKSLES-----------SKLHYSSSGNSLFVVLTDAVIIVHVQED-D 388
Query: 404 SNYPFKRKWEDIIRFNKDMK-----------VLG-YDCNSSSLYITDGRTCALKLETFEK 451
+ + WE++IR N ++ VLG Y + S +I + ++E
Sbjct: 389 KDIVSRTSWEEVIRMNTNVSGGIFMSTCYKYVLGKYSIPTESCFIATPYSGIAEIEVHSL 448
Query: 452 YENSDFQEIRFIKSHVDQYVYFGGQSTXXXXXXXXXXXXXXQPQEIETDIQMSSDEIKYA 511
++ + + +KS +++ V++ +P E+E I +I +
Sbjct: 449 EHPANNESL--VKSKLEEAVFYSFLPGNPIDFSCNYLRSIKKP-ELERIIVDLGMDILNS 505
Query: 512 LSNHIPPVANNPMKHLKIRSELFERLLTFVAHNFLD-QTSSQFKLDLISDFEMIKCLESL 570
S H+PP+ + M+HL R L+ ++ LD +KL ++ E + L
Sbjct: 506 RSTHLPPLFASLMQHLSCRLNSLNNLVRYIRSMSLDVDRQVLYKLRVMG--EKCNSVRYL 563
Query: 571 YSLLPQQPTSISQA-------WKTVLSESSYQSDSDFISDGIVKIPELLGAFLKHLKDLM 623
++ + + +++S + ++ S SS + ++ I I +L+ + D
Sbjct: 564 WNTIDTEFSTVSHSLIFQRIIYRLTQSASSDNALREWFLHNIESIDQLIAQAHEFCIDSG 623
Query: 624 KSTNETMLK-SQIAKLINQLVYHSILEECENSYR---YGLFQMSPDDVSKNIPWFTTNSI 679
E L+ + N+++ ++ +YR ++++S D + +PW +T
Sbjct: 624 SRVQELPLEVLDVIMEANEVIL--AIQSSALAYRRESQKIYKLSIDTFGEEVPWTSTPET 681
Query: 680 IENLNDIFFLLRHA-------SEDXXXXXXXXXXXXXXXXXXXYMVRQITM--------- 723
+ L F L R A + D V+ + +
Sbjct: 682 LVLLCRQFELTRSALVQSHQGTSDVENTFKIKDKGVLRNVVSNLEVQLVALTEVCFDAYS 741
Query: 724 ----WIDANAITDSS----YKQFFEENHLLWNRVLRDVNRSQDSILITDFYQDFEGLVET 775
WI+ D+S K+ F N W + L D+ + +I I + Y D+ LVE
Sbjct: 742 ERIRWIEQRCGKDASEIQDVKEAFAVNRRFWVQTLSDIGKGSSAIRIAEKYSDYRSLVEL 801
Query: 776 LQSLPKITAFS-LYDEFFQKYGYDFAKSLFSYYIKEGLWDDLYESFGETHSEYLDQFLGE 834
L + + + + +G FA L+ Y+++ G+ +L S + YL QF +
Sbjct: 802 CYQLYEDNELTDALNNYLDLFGIKFAFILYDYFVENGMALELLNS-DRFNKSYLKQFF-K 859
Query: 835 CPEYGKVKWIGNIYCHKYDEATTTLLSISTGTQSLGQDLKQRQAQLSIAKLTALA----- 889
+Y ++ W+ ++ YD A+ LL ++T + L + +++++LS++KL A
Sbjct: 860 SRDYNQISWMHDMRLGDYDAASHRLLQLATKQEKL---VDKKESELSLSKLFLYAVPSNS 916
Query: 890 AEVQDL----EKLNEIQTEL----------------------------DLIDGQI--LLS 915
++DL +KL ++ + DLI ++ +++
Sbjct: 917 GNIRDLVLVEQKLEQLHIQKMVSKSVMPVVERLRSQGKKYQLVEAVVDDLIGAKVAPVIA 976
Query: 916 QELQESTLNPLFKDFTTYRT-LFKYLSDAVKRRESLSVPHMIELFTLLNQDTSYGFALSI 974
+++ + + T L +YLS ++ RRE L + + T Y AL +
Sbjct: 977 RQVMQRVVKKFIAGQVVEATELLEYLSFSLYRREDL----------VEGEVTDYYLALRL 1026
Query: 975 LAIDHTLPTEVKKYCEYQIWRRCLLSD--------ENAVRQTLRVFFKQQFYHQSIELPI 1026
L + L + K++ E IWRR +L D + + F+ ++++E
Sbjct: 1027 L-LTTRLTDDAKRFYENTIWRRAVLHDNWIQVLDTQGKNDAIIETQFRMSALYRTLEAVT 1085
Query: 1027 IDNLANEKLI 1036
I+ L +E LI
Sbjct: 1086 INGLFHEGLI 1095
>SPBP35G2.06c [YU] KOG4121 Nuclear pore complex Nup133 component (sc Nup133)
Length = 1142
Score = 72.8 bits (177), Expect = 3e-12
Identities = 171/867 (19%), Positives = 328/867 (37%), Gaps = 92/867 (10%)
Query: 111 EYESWSRPPLCCFTWPSVLERDLKSSPGLFLVNSRSGDIQFYEDVDTVSQVASFLTQTKR 170
+ +S + PPL F P + PG L+ SGD+ ++E ++ S
Sbjct: 114 DQQSLNSPPLAGFVKPD----PTSAEPGFLLLWPASGDLFYWERTSLMNVEVSAPEFPGF 169
Query: 171 HKSVLKLRDGEYVENLINAEPAGILLSTNYGRVEFCTIRDANGKPFVSLKQT-----IIK 225
++L+ E +NL++AEPAG L+ + GR+ ++D++G + ++ I
Sbjct: 170 LHYRIQLKRYESCKNLVSAEPAGFFLTLSSGRLLHLNLKDSDGNSSIQVRILFPGLHIPN 229
Query: 226 CQVGIFSSMSK--SKRVVSLKLGEISGKGERIVSILTQGGRYQVWNLSSVGNCYKKIDLN 283
C + +++S+ S RV+ ++ G I G GER+V + G ++W + G+ + +
Sbjct: 230 CLLSLYNSIFAFTSGRVIPIRCGSIKGPGERLVYSMHSGSTLRIWEIFGTGDHHLLRGFD 289
Query: 284 VQSEISQAVLEHFPLATSTLKLLDCHPLSGDSDAYLFLSFLA-ESNETHYLLSTIIVDED 342
+ I ++ E F L +LD SD Y ++ N + + + II+ +
Sbjct: 290 IYDIILDSIQESFSYVNRFL-ILDFS--VSTSDPYTLACLVSWRDNASLFNYAAIIISFN 346
Query: 343 TNSFAIFSTY-RLNTYTRPLSDEFAPRLIVPTAYTKEHLPITSVYTLFSDAAVLTQT--- 398
S + + +Y + R+ +P+ T + +F T
Sbjct: 347 HQMIPHVSQFCHVRSYLSAILPSVC-RIFLPSPGTVVFCVFDVTFAMFHKVRGKEGTFYV 405
Query: 399 -SSKLDSNYPFKRK-----WEDIIRFNKDMKVLGYDCNSSSLYITDGRTCALKLETFEKY 452
+ + +N K + ED I +L + + +T+G +++E+ Y
Sbjct: 406 EENLVVNNLTSKSRVLDFGMEDAIYEKSTHTLLK---TPALILLTEGHG-IIRIESSASY 461
Query: 453 ENSDFQEIRFIKSHVDQYVYFGGQSTXXXXXXXXXXXXXXQPQEIETDIQMSSDEIKYAL 512
+ F +++S + Q F QS + +S E+ +
Sbjct: 462 LKTSFSATTYLRSRLSQ---FASQSPLFREQFLL---------NFDFTYNLSESEVYNTV 509
Query: 513 SNHIPPVANNP-MKHLKIRSELFERL-----LTFVAHNFLDQTSSQFKLDLISDFEMIKC 566
N + P K++ + L + + VA +L + S +L L
Sbjct: 510 FNLCDELYTTPCKKNMSVLETLHSQQRDLMEIVLVAFKYLHLSPSN-RLQLWLKVAYCSA 568
Query: 567 LESLYSLLPQQPTSISQAWKTVLSESSYQSDSDFISDGIVKIPELLGAFLKHLK---DLM 623
L LY LL +S + VL S +D I + +K + L+HL +
Sbjct: 569 LADLYELL-MCNEKMSNLLRRVL---KIMSSNDNIDELFLKKCLNINLLLQHLSSEYSQI 624
Query: 624 KSTNETMLKSQIAKLINQLVYHSILEECENSYRYGLFQ--MSPDDVSKNIPWFTTNSIIE 681
+ + + +I ++N + + E +YR P V ++ W ++
Sbjct: 625 SGQDASNAQKRIEVIVNVNNVYGTIFGNELAYRQEKISKFTDPSSVIRSDLWTVE---VD 681
Query: 682 NLNDIFFLLRHASEDXXX--XXXXXXXXXXXXXXXXYMVRQITMWIDANAI--------T 731
+L+ LLR ED + +QI ++ N + T
Sbjct: 682 HLD----LLRKQIEDSISLHRDVRDSKDAHILKVSNKLRKQILHLVEQNCLLIFDLHNGT 737
Query: 732 DSS------YKQFFEE---NHLLWNRVLRDVNRSQDSILITDFYQDFEGLVETLQSL-PK 781
D + +++F EE + W + L V + +I + + +DF+ +V L L PK
Sbjct: 738 DDTIVKSHNFQKFEEEFGNSRKEWLQYLASVGYLEKAIDLAEKVKDFQTMVILLDCLDPK 797
Query: 782 ITAFSLYDE--FFQKYGYDFAKSLFSYYIKEGLWDDLYESFGETHSEYLDQFLGECPEYG 839
L + KY +FA +F Y + + L F E S + F E +
Sbjct: 798 DPTIKLQKQKACLNKYKEEFADVIFFYLVDTEQYASLLHDFLEYQSLLISFF--EANDLL 855
Query: 840 KVKWIGNIYCHKYDEATTTLLSISTGTQSLGQDLKQRQAQLSIAKLTALAAEVQDLEKLN 899
+ WI Y A+ L ++ + + +QL++AKL + ++ +
Sbjct: 856 NLLWIFEAQNGNYGRASEILFRDCEPEEN---RIIGKISQLALAKLYSWTNRIEGENIIL 912
Query: 900 EIQTELDLIDGQILLSQELQESTLNPL 926
+TE+ ILL QE L+ L
Sbjct: 913 SNETEVIENHLAILLIQEQYAEMLSSL 939
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.134 0.387
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 62,761,434
Number of Sequences: 60738
Number of extensions: 2669136
Number of successful extensions: 7235
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 7214
Number of HSP's gapped (non-prelim): 5
length of query: 1106
length of database: 30,389,216
effective HSP length: 117
effective length of query: 989
effective length of database: 23,282,870
effective search space: 23026758430
effective search space used: 23026758430
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)