ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI2075 good J KOG3031 Translation, ribosomal structure and biogenesis Protein required for biogenesis of the ribosomal 60S subunit
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI2075 706289 707320 344
(344 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKR081c [J] KOG3031 Protein required for biogenesis of the ribos... 519 e-147
SPAC926.08c [J] KOG3031 Protein required for biogenesis of the r... 300 3e-81
Hs20551627 [J] KOG3031 Protein required for biogenesis of the ri... 220 2e-57
7300355 [J] KOG3031 Protein required for biogenesis of the ribos... 183 4e-46
CE24439 [J] KOG3031 Protein required for biogenesis of the ribos... 174 1e-43
At3g23620 [J] KOG3031 Protein required for biogenesis of the rib... 123 4e-28
>YKR081c [J] KOG3031 Protein required for biogenesis of the ribosomal 60S
subunit
Length = 344
Score = 519 bits (1336), Expect = e-147
Identities = 256/345 (74%), Positives = 300/345 (86%), Gaps = 2/345 (0%)
Query: 1 MIRTVKPKNARAKRALDKKEAKLVENVKQALFIPGQTSTKLLHDSMVDLSALKKPDIKRF 60
MIRTVKPKNARAKRAL K+EAKLVENVKQALFIPGQ+ K LHD MVDLSALKKPD+KRF
Sbjct: 1 MIRTVKPKNARAKRALVKREAKLVENVKQALFIPGQSCNKNLHDIMVDLSALKKPDMKRF 60
Query: 61 SKKNNIRPFEDYEPLEFLSEKNDTSLMVLSTHSKKRRNNLTFIRTFGYKIYDIIELQIAE 120
++KN+I PFED PLEF SEKND SLMVL T SKKR+NN+TFIRTFGYKIYD+IEL +A+
Sbjct: 61 NRKNDIHPFEDMSPLEFFSEKNDCSLMVLMTSSKKRKNNMTFIRTFGYKIYDMIELMVAD 120
Query: 121 NYKLLSDFRKQTFNVGLKPMFSFQGAAFEQHPVYQHIKSLFLDFFRGQNTTLQDVAGLQH 180
N+KLLSDF+K TF VGLKPMF+FQGAAF+ HPVY+ IKSLFLDFFRG++T LQDVAGLQH
Sbjct: 121 NFKLLSDFKKLTFTVGLKPMFTFQGAAFDTHPVYKQIKSLFLDFFRGESTDLQDVAGLQH 180
Query: 181 IISITVQGDFQDGEPLPNVLFRVYHLKTYKSEQGGKKLPRVELEEIGPRFDFKIGRIHTP 240
+IS+T+QGDFQDGEPLPNVLFRVY LK+YKS+QGGK+LPR+EL EIGPR DFKIGRIHTP
Sbjct: 181 VISMTIQGDFQDGEPLPNVLFRVYKLKSYKSDQGGKRLPRIELVEIGPRLDFKIGRIHTP 240
Query: 241 SPEMVKEAHKKPRQLEVRVAKNVEIDTMGDKIGRVHVGKQDLGQLQTRKMKGLKAKYDQV 300
SP+MV EAHKKP+QLE++ KNVE+D MGDK+GR+H+GKQDLG+LQTRKMKGLK+K+DQ
Sbjct: 241 SPDMVTEAHKKPKQLEMKTKKNVELDIMGDKLGRIHMGKQDLGKLQTRKMKGLKSKFDQG 300
Query: 301 DSDME-EYLEEVEGDLDQENVDDTYGEEFVTATDIDAPPAKKQKK 344
+ + E E+ E + + Y EEFV+ATDI+ P AK+QKK
Sbjct: 301 TEEGDGEVDEDYEDEASYSDDGQEYEEEFVSATDIE-PSAKRQKK 344
>SPAC926.08c [J] KOG3031 Protein required for biogenesis of the ribosomal 60S
subunit
Length = 317
Score = 300 bits (767), Expect = 3e-81
Identities = 160/317 (50%), Positives = 214/317 (67%), Gaps = 8/317 (2%)
Query: 1 MIRTVKPKNARAKRALDKKEAKLVENVKQALFIPGQTSTKLLHDSMVDLSALKKPDIKRF 60
M+R VKPKNAR KRAL+K+E KLVE K A+F+ G ++++ D + D+ ALKKP F
Sbjct: 1 MLRQVKPKNARTKRALEKREPKLVEGAKTAIFLRGNATSQISLDVLGDIHALKKPFSVNF 60
Query: 61 SKKNNIRPFEDYEPLEFLSEKNDTSLMVLSTHSKKRRNNLTFIRTFGYKIYDIIELQIAE 120
KKNNI PFED LEF SEKND +L V++TH+KKR +NLT++R F Y++ D+IEL I
Sbjct: 61 QKKNNILPFEDASSLEFFSEKNDAALAVMATHNKKRPHNLTWVRFFNYRVLDMIELGIV- 119
Query: 121 NYKLLSDFRKQTFNVGLKPMFSFQGAAFEQHPVYQHIKSLFLDFFRGQNTTLQDVAGLQH 180
NYK + F G KPM FQG F+ HP Y+HIKSLFLDFFRG+ D AGL +
Sbjct: 120 NYKSIQSFSATPIVPGTKPMILFQGPVFDAHPTYRHIKSLFLDFFRGEPIQKLDSAGLSY 179
Query: 181 IISIT-VQGDFQDGEPLPNVLFRVYHLKTYKSEQGGKKLPRVELEEIGPRFDFKIGRIHT 239
+I ++ + + +PLP V FRVY K K++ LPRVELEE+GPR DF I R+
Sbjct: 180 VIVVSAAEAQEDETKPLPLVHFRVYGTKLLKTK---TNLPRVELEEMGPRIDFNIRRVQP 236
Query: 240 PSPEMVKEAHKKPRQLEVRVAKNVEIDTMGDKIGRVHVGKQDLGQLQTRKMKGLKAKYDQ 299
++++EA KKP+ E + KNV++D +G+K+GR+HV +QDLG LQTRKMKGLK ++
Sbjct: 237 AESDVLEEALKKPKTQEPKPKKNVDVDIIGNKVGRIHVDQQDLGNLQTRKMKGLKRSVEE 296
Query: 300 -VDSDMEEYLEEVEGDL 315
DS+ EE E+E D+
Sbjct: 297 REDSENEEV--EIEEDV 311
>Hs20551627 [J] KOG3031 Protein required for biogenesis of the ribosomal 60S
subunit
Length = 306
Score = 220 bits (561), Expect = 2e-57
Identities = 127/311 (40%), Positives = 179/311 (56%), Gaps = 13/311 (4%)
Query: 3 RTVKPKNARAKRALDKKEAKLVENVKQALFIPGQTSTKLLHDSMVDLSALKKPDIKRFSK 62
R VKPK RAKR L+K+E KL EN+K A+ I G + + + D+ ALKKP + K
Sbjct: 6 RVVKPKTKRAKRFLEKREPKLNENIKNAMLIKGGNANATVTKVLKDVYALKKPYGVLYKK 65
Query: 63 KNNIRPFEDYEPLEFLSEKNDTSLMVLSTHSKKRRNNLTFIRTFGYKIYDIIELQIAENY 122
KN RPFED LEF S+K+D SL + +H+KKR NNL R + Y + D+IEL I EN+
Sbjct: 66 KNITRPFEDQTSLEFFSKKSDCSLFMFGSHNKKRPNNLVIGRMYDYHVLDMIELGI-ENF 124
Query: 123 KLLSDFRKQTFNVGLKPMFSFQGAAFEQHPVYQHIKSLFLDFFRGQNTTLQDVAGLQHII 182
L D + G KPM F G F+ Y+ +KSL +DFFRG + +AGL++++
Sbjct: 125 VSLKDIKNSKCPEGTKPMLIFAGDDFDVTEDYRRLKSLLIDFFRGPTVSNIRLAGLEYVL 184
Query: 183 SITVQGDFQDGEPLPNVLFRVYHLKTYKSEQGGKKLPRVELEEIGPRFDFKIGRIHTPSP 242
T + FR Y L KS G + PR+ELEE+GP D + R H S
Sbjct: 185 HFTALNG--------KIYFRSYKLLLKKS---GCRTPRIELEEMGPSLDLVLRRTHLASD 233
Query: 243 EMVKEAHKKPRQLEVRVAKNVEIDTMGDKIGRVHVGKQDLGQLQTRKMKGLKAK-YDQVD 301
++ K + K P+ L+ + KN+ DT G GR+H+ KQDL +LQTRKMKGLK + +++
Sbjct: 234 DLYKLSMKMPKALKPKKKKNISHDTFGTTYGRIHMQKQDLSKLQTRKMKGLKKRPAERIT 293
Query: 302 SDMEEYLEEVE 312
D E+ + ++
Sbjct: 294 EDHEKKSKRIK 304
>7300355 [J] KOG3031 Protein required for biogenesis of the ribosomal 60S
subunit
Length = 289
Score = 183 bits (464), Expect = 4e-46
Identities = 100/264 (37%), Positives = 155/264 (57%), Gaps = 11/264 (4%)
Query: 31 LFIPGQTSTKLLHDSMVDLSALKKPDIKRFSKKNNIRPFEDYEPLEFLSEKNDTSLMVLS 90
LF+ G+ + M DL ALKKP +K ++KN+I PF+D LEFL+ KND +L
Sbjct: 2 LFLDGRKCGGNVKLCMKDLQALKKPLVKVLNRKNDITPFDDPSSLEFLTMKNDAALFTFG 61
Query: 91 THSKKRRNNLTFIRTFGYKIYDIIELQIAENYKLLSDFRKQTFNVGLKPMFSFQGAAFEQ 150
+ SKKR +N+ R F ++ D+ EL I + Y+ +S+F+ + +KP F G + Q
Sbjct: 62 STSKKRPDNIILGRIFENEVLDMFELGI-KRYQAISEFKNEKIGACVKPCLVFNGPKWAQ 120
Query: 151 HPVYQHIKSLFLDFFRGQNTTLQDVAGLQHIISITVQGDFQDGEPLPNVLFRVYHLKTYK 210
+ +++LF+D F+ + + G++H++S TV D N+L R Y + K
Sbjct: 121 TEELRRLRNLFIDTFQREKVDSIRLQGIEHVLSFTVTDDM-------NILMRSYRILLKK 173
Query: 211 SEQGGKKLPRVELEEIGPRFDFKIGRIHTPSPEMVKEAHKKPRQLEVRVAKNVEIDTMGD 270
S G++ PR+ELEEIGP DF I R S ++ K+A K+P+QL+V KN+ D +G+
Sbjct: 174 S---GQRTPRIELEEIGPSADFSIRRTKIASEDLYKQARKQPKQLKVGKKKNISTDALGN 230
Query: 271 KIGRVHVGKQDLGQLQTRKMKGLK 294
GRVH+GKQ G +QTR++K L+
Sbjct: 231 TKGRVHLGKQQTGSIQTRRVKALR 254
>CE24439 [J] KOG3031 Protein required for biogenesis of the ribosomal 60S
subunit
Length = 297
Score = 174 bits (442), Expect = 1e-43
Identities = 113/309 (36%), Positives = 164/309 (52%), Gaps = 12/309 (3%)
Query: 1 MIRTVKPKNARAKRALDKKEAKLVENVKQALFIPGQTSTKLLHDSMVDLSALKKPDIKRF 60
M+R K K + KR L + +K VEN K+ALF G + +++ +M+DL +KKP +
Sbjct: 1 MVRVEKIKTKKGKRVLVNRASKTVENDKKALFCRGAKTNEIISHAMMDLFDMKKPLTTKM 60
Query: 61 SKKNNIRPFEDYEPLEFLSEKNDTSLMVLSTHSKKRRNNLTFIRTFGYKIYDIIELQIAE 120
K N FED P+ K DTSL VL ++SKK+ N LTF RT+ ++ D+ EL+I
Sbjct: 61 DKHNPYHLFEDETPIVRAGSKFDTSLFVLGSNSKKKPNCLTFGRTYDGQLLDMAELRIT- 119
Query: 121 NYKLLSDFRKQTFNVGLKPMFSFQGAAFEQHPVYQHIKSLFLDFFRGQNTTLQDVAGLQH 180
+YK S+F +G KP +GAAFE + I +L +D+FRG + GL+
Sbjct: 120 SYKSSSNFEAAKMTLGSKPCVILEGAAFESDGDMKRIGNLMVDWFRGPKVDTVRLEGLET 179
Query: 181 IISITVQGDFQDGEPLPNVLFRVYHLKTYKSEQGGKKLPRVELEEIGPRFDFKIGRIHTP 240
+I T + N+ RVY KS PRVEL E+GP F++ R
Sbjct: 180 VIVFTALDE-------TNLALRVYRPMLKKS---ATATPRVELAEMGPSISFEVMRKKLA 229
Query: 241 SPEMVKEAHKKPRQLEVRVAKNVEIDTMGDKIGRVHVGKQDLGQLQTRKMKGLKAKYDQV 300
+ K A KKP+ L + KN+ D G+++ RVHVGKQ +QTRK+K L+ K V
Sbjct: 230 DDALFKLACKKPKALMKKRRKNLSEDVFGNQLARVHVGKQRTDDIQTRKVKALR-KTPLV 288
Query: 301 DSDMEEYLE 309
++ E +E
Sbjct: 289 EAAPENAIE 297
>At3g23620 [J] KOG3031 Protein required for biogenesis of the ribosomal 60S
subunit
Length = 269
Score = 123 bits (308), Expect = 4e-28
Identities = 88/273 (32%), Positives = 141/273 (51%), Gaps = 20/273 (7%)
Query: 46 MVDLSALKKPDIKRFSKKN-NIRPFED--YEPLEFLSEKNDTSLMVLSTHSKKRRNNLTF 102
M +L LKK R+S++N NIRPFE LEF S+K D S+ V +H+KKR +NL
Sbjct: 1 MTELYRLKKGGAIRYSRRNENIRPFESGGETSLEFFSQKTDCSIFVYGSHTKKRPDNLVL 60
Query: 103 IRTFGYKIYDIIELQIAENYKLLSDF---RKQTFNVGLKPMFSFQGAAFEQHPVYQHIKS 159
R + +++YD+IE+ I EN+K L F +K + G KP F G FE +H+K
Sbjct: 61 GRMYDHQVYDLIEVGI-ENFKSLRAFSYDKKFAPHEGTKPFICFIGEGFENVSELKHLKE 119
Query: 160 LFLDFFRGQNTTLQDVAGLQHIISITVQGDFQDGEPLPNVLFRVYHLKTYKSEQGGKKLP 219
+ D FRG+ ++ GL + P +F + K ++ G +P
Sbjct: 120 VLTDLFRGEVVDNLNLTGLDRAYVCSAIS--------PTKVFLTHC--ALKLKKSGSIVP 169
Query: 220 RVELEEIGPRFDFKIGRIHTPSPEMVKEAHKKPRQLEVRVAKNVEIDTMGDKIGRVHVGK 279
R+EL E+GP D I R P+ ++KEA + + + KNV+ D + K G++++
Sbjct: 170 RMELVEVGPSMDLVIRRNRLPNDSLMKEAMRTSKDKPKKKEKNVDQDAVLGKTGKIYMPD 229
Query: 280 QDLGQLQT-RKMKGLKAKYDQVDSDMEEYLEEV 311
Q L +++ K KG +K ++ D+ ++ E V
Sbjct: 230 QKLKEMKLFDKSKG--SKRERKDAKLKHKEETV 260
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.136 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,520,825
Number of Sequences: 60738
Number of extensions: 919557
Number of successful extensions: 3141
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3121
Number of HSP's gapped (non-prelim): 7
length of query: 344
length of database: 30,389,216
effective HSP length: 107
effective length of query: 237
effective length of database: 23,890,250
effective search space: 5661989250
effective search space used: 5661989250
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)