ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI2079 good H KOG0089 Coenzyme transport and metabolism Methylenetetrahydrofolate dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI2079 708441 707464 -326
(326 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKR080w [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/meth... 501 e-142
SPBC1711.04 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/... 360 1e-99
7299133 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/meth... 86 1e-16
At4g00620 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/me... 80 5e-15
YBR084w [H] KOG4230 C1-tetrahydrofolate synthase 75 1e-13
At3g12290 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/me... 72 8e-13
Hs5729935 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/me... 72 1e-12
SPBC839.16 [H] KOG4230 C1-tetrahydrofolate synthase 69 7e-12
CE25047 [H] KOG4230 C1-tetrahydrofolate synthase 69 7e-12
YGR204w [H] KOG4230 C1-tetrahydrofolate synthase 62 1e-09
At4g00600 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/me... 62 1e-09
At2g38660 [H] KOG0089 Methylenetetrahydrofolate dehydrogenase/me... 62 1e-09
SPBC2G2.08 [H] KOG4230 C1-tetrahydrofolate synthase 52 2e-06
>YKR080w [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 320
Score = 501 bits (1291), Expect = e-142
Identities = 241/311 (77%), Positives = 274/311 (87%), Gaps = 1/311 (0%)
Query: 5 KPGRTILASSISKTYVQEITGKVESLRNIRP-DGPLLVGFLANNDPAAEMYASWTQKTCE 63
KPGRTILAS +++T+ EI VE + GPLLVGFLANNDPAA+MYA+WTQKT E
Sbjct: 3 KPGRTILASKVAETFNTEIINNVEEYKKTHNGQGPLLVGFLANNDPAAKMYATWTQKTSE 62
Query: 64 SMGFRYELRRIEDRDFLEEAIIDANQDDSVDGIMIYFPVFGNAQDQYLQQVVSREKDVEG 123
SMGFRY+LR IED+DFLEEAII AN DDSV+GIM+YFPVFGNAQDQYLQQVV +EKDVEG
Sbjct: 63 SMGFRYDLRVIEDKDFLEEAIIQANGDDSVNGIMVYFPVFGNAQDQYLQQVVCKEKDVEG 122
Query: 124 LNHVYYQNMYHNIRYLDDQKQLKSILPCTPLAVVKILEYLKVYNNLLPEGNRLYGKKCVV 183
LNHVYYQN+YHN+RYLD + +LKSILPCTPLA+VKILE+LK+YNNLLPEGNRLYGKKC+V
Sbjct: 123 LNHVYYQNLYHNVRYLDKENRLKSILPCTPLAIVKILEFLKIYNNLLPEGNRLYGKKCIV 182
Query: 184 VNRSEIVGRPLAALLANDGADVFSVDINNIQKFTRGQGLKFTKHHVEDLGAYSEELLKEC 243
+NRSEIVGRPLAALLANDGA V+SVD+NNIQKFTRG+ LK KHHVEDLG YSE+LLK+C
Sbjct: 183 INRSEIVGRPLAALLANDGATVYSVDVNNIQKFTRGESLKLNKHHVEDLGEYSEDLLKKC 242
Query: 244 CKDADVIITGVPGEKYKFPTDYIKEGAICINFSSEKNFDESVKSKASLYVPSTGKVTISM 303
D+DV+ITGVP E YKFPT+YIKEGA+CINF+ KNF + VK KASLYVP TGKVTI+M
Sbjct: 243 SLDSDVVITGVPSENYKFPTEYIKEGAVCINFACTKNFSDDVKEKASLYVPMTGKVTIAM 302
Query: 304 LLRNMLRLVEN 314
LLRNMLRLV N
Sbjct: 303 LLRNMLRLVRN 313
>SPBC1711.04 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 320
Score = 360 bits (925), Expect = 1e-99
Identities = 184/305 (60%), Positives = 232/305 (75%), Gaps = 10/305 (3%)
Query: 8 RTILASSISKTYVQEITGKVESLRNIRPDGPLLVGFLANNDPAAEMYASWTQKTCESMGF 67
+ + AS +++T+V+++ V N+ P LVGFL+N+DPAA MYA WT KTC +GF
Sbjct: 14 KVVYASRVAETFVEQLKQHV----NLFEFAPKLVGFLSNSDPAARMYADWTNKTCTEIGF 69
Query: 68 RYELRRIEDRDFLEEAIIDANQDDSVDGIMIYFPVFGNAQDQYLQQVVSREKDVEGLNHV 127
+YELR + +D LE+AI++AN D SV+GIMIYFPVF + QDQYLQQVVS +KDVEGL H
Sbjct: 70 QYELREVP-KDDLEDAIVEANNDPSVNGIMIYFPVFNDGQDQYLQQVVSPDKDVEGLCHK 128
Query: 128 YYQNMYHNIRYLDDQKQLKSILPCTPLAVVKILEYLKVYNNLLPEGNRLYGKKCVVVNRS 187
Y NMYHNIR+LD +K KSILPCTPLA+VKILEYL VYN ++ GNRLYGK +VNRS
Sbjct: 129 YVMNMYHNIRHLDPEKTKKSILPCTPLAIVKILEYLGVYNKIINYGNRLYGKTITIVNRS 188
Query: 188 EIVGRPLAALLANDGADVFSVDINNIQKFTRGQGLKFTKHHVEDLGAYSEELLKECCKDA 247
EIVGRPLAALLANDGA V+SVDI+N+Q FTRG G++ KH V D + L++ +
Sbjct: 189 EIVGRPLAALLANDGAKVYSVDIHNVQCFTRGAGIRSKKHDVAD----TNFKLEDVAPIS 244
Query: 248 DVIITGVPGEKYKFPTDYIKEGAICINFSSEKNFD-ESVKSKASLYVPSTGKVTISMLLR 306
DVII GVP YKFP++ +++GA+CI FSSEKNFD S+K A +YVPS GKVTI+MLLR
Sbjct: 245 DVIICGVPSANYKFPSNLVRDGAVCICFSSEKNFDAASLKEHAGIYVPSIGKVTIAMLLR 304
Query: 307 NMLRL 311
N++RL
Sbjct: 305 NLIRL 309
>7299133 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 303
Score = 85.5 bits (210), Expect = 1e-16
Identities = 86/335 (25%), Positives = 139/335 (40%), Gaps = 66/335 (19%)
Query: 17 KTYVQEI-TGKVESLRNIRPDG---PLLVGFLANNDPAAEMYASWTQKTCESMGFRYELR 72
K QE+ T L+ + G P L + DPA+E Y + C +G E +
Sbjct: 8 KAIAQEVRTQLAHELKGMEAAGYPKPHLTAVIVGEDPASEKYVANKMVACREVGISSETK 67
Query: 73 RIEDRDFLEEA---IIDANQDDSVDGIMIYFPVFGNAQDQYLQQVVSREKDVEGLNHVYY 129
R+ EE I D N+D V GI++ PV + ++ + V +KDV+G N V
Sbjct: 68 RLPASTTQEELLQLIADLNKDPQVTGILVQLPVPEHINERTICNAVDVDKDVDGFNEV-- 125
Query: 130 QNMYHNIRYLDDQKQLKSILPCTPLAVVKILEYLKVYNNLLPEGNRLYGKKCVVVNRSEI 189
NI +++ +P TPL V ++LE++K+ G+ VVV RS+
Sbjct: 126 -----NIG--RTALDMEANIPATPLGVKRLLEHMKI---------ETLGRNAVVVGRSKN 169
Query: 190 VGRPLAALLANDG---ADVFSVDINNIQKFTRGQGLKFTKHHVEDLGAYSEELLKECCKD 246
V P+A LL DG + ++T + L +H C+
Sbjct: 170 VSLPMAILLHADGKYATKAMDATVTICHRYTPPKEL--ARH----------------CRQ 211
Query: 247 ADVIITGVPGEKYKFPTDYIKEGAICINFSSEKNFDES--------------VKSKASLY 292
AD+I+ V G+ D +K GA I+ + DES V+ A
Sbjct: 212 ADIIVVAV-GKPGLITKDMVKPGACVIDVGINRIKDESTGQFKLVGDVDFEEVRQVAGHI 270
Query: 293 VP---STGKVTISMLLRNMLRLVENKQKLQNRSAS 324
P G +T++ML+ N L+ +++ +R +S
Sbjct: 271 TPVPGGVGPMTVAMLMHNTLKAA--RKQFDDRKSS 303
>At4g00620 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 360
Score = 79.7 bits (195), Expect = 5e-15
Identities = 86/330 (26%), Positives = 138/330 (41%), Gaps = 65/330 (19%)
Query: 3 TEKPGRTIL--ASSISKTYVQEITGKVESLRNIRPDGPLLVGFLANNDPAAEMYASWTQK 60
T+ G I+ +++K EIT +V ++ P L L + + Y +K
Sbjct: 63 TKSEGGAIVIDGKAVAKKIRDEITIEVSRMKESIGVIPGLAVILVGDRKDSATYVRNKKK 122
Query: 61 TCESMGFR-YELRRIEDRDFLE--EAIIDANQDDSVDGIMIYFPVFGNAQDQYLQQVVSR 117
C+S+G + +E+R ED E +++ N D SV GI++ P+ + +Q + VS
Sbjct: 123 ACDSVGIKSFEVRLAEDSSEEEVLKSVSGFNDDPSVHGILVQLPLPSHMDEQNILNAVSI 182
Query: 118 EKDVEGLNHVYYQNMYHNIRYLDDQKQLKSILPCTPLAVVKILEYLKVYNNLLPEGNRLY 177
EKDV+G + + NI L + + +PCTP +++L + +
Sbjct: 183 EKDVDGFHPL-------NIGRLAMRGREPLFVPCTPKGCIELLHRYNI---------EIK 226
Query: 178 GKKCVVVNRSEIVGRPLAALLANDGADVFSVDINNIQKFTRGQGLKFTKHHVEDLGAYSE 237
GK+ VV+ RS IVG P A LL + A V + I T+
Sbjct: 227 GKRAVVIGRSNIVGMPAALLLQREDATV-----SIIHSRTKNP----------------- 264
Query: 238 ELLKECCKDADVIITGVPGEKYKFPTDYIKEGAICINFSSEKNFD--------------- 282
+E ++AD+II+ V G+ +IK GA+ I+ D
Sbjct: 265 ---EEITREADIIISAV-GQPNMVRGSWIKPGAVLIDVGINPVEDPSAARGYRLVGDICY 320
Query: 283 ESVKSKASLYVP---STGKVTISMLLRNML 309
E AS P G +TI+MLL N L
Sbjct: 321 EEASKVASAITPVPGGVGPMTIAMLLSNTL 350
>YBR084w [H] KOG4230 C1-tetrahydrofolate synthase
Length = 975
Score = 75.1 bits (183), Expect = 1e-13
Identities = 70/249 (28%), Positives = 105/249 (42%), Gaps = 62/249 (24%)
Query: 80 LEEAIIDANQDDSVDGIMIYFPVFGNAQDQYLQQVVSREKDVEGLNHVYYQNMYHNIRYL 139
L + I D N DDS+ G++I P+ + + + V +KDV+G + +N L
Sbjct: 112 LLKKISDINDDDSIHGLLIQLPLPRHLDETTITNAVDFKKDVDGFHR-------YNAGEL 164
Query: 140 DDQKQLKSILPCTPLAVVKILEYLKVYNNLLPEGNRLYGKKCVVVNRSEIVGRPLAALLA 199
+ +PCTP +K+LE V +L GK VV+ RS IVG P+A+LL
Sbjct: 165 AKKGGKPYFIPCTPYGCMKLLEEAHV---------KLDGKNAVVLGRSSIVGNPIASLLK 215
Query: 200 NDGADVFSVDINNIQKFTRGQGLKFTKHHVEDLGAYSEELLKECCKDADVIITGVPGEKY 259
N A V TR + E AD++I +Y
Sbjct: 216 NANATV-----TVCHSHTRN--------------------IAEVVSQADIVIAACGIPQY 250
Query: 260 KFPTDYIKEGAICINFS----------------SEKNFDESVKSKASLYVP---STGKVT 300
+D+IKEGA+ I+ + +FD SVK K S P G +T
Sbjct: 251 -VKSDWIKEGAVVIDVGINYVPDISKKSGQKLVGDVDFD-SVKEKTSYITPVPGGVGPMT 308
Query: 301 ISMLLRNML 309
++ML+ N+L
Sbjct: 309 VAMLVSNVL 317
>At3g12290 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 299
Score = 72.4 bits (176), Expect = 8e-13
Identities = 77/328 (23%), Positives = 141/328 (42%), Gaps = 65/328 (19%)
Query: 2 STEKPGRTILASSISKTYVQEITGKVESLRNIRPDGPLLVGFLANNDPAAEMYASWTQKT 61
S++ + I +I+ T EI +V L P L + + ++ Y + +K
Sbjct: 4 SSDHTAKIIDGKAIAHTIRSEIAEEVRGLSEKHGKVPGLAVVIVGSRKDSQTYVNTKRKA 63
Query: 62 CESMGFR-YEL---RRIEDRDFLEEAIIDANQDDSVDGIMIYFPVFGNAQDQYLQQVVSR 117
C +G + +++ + + D + + + + N + V GI++ P+ + ++++ +S
Sbjct: 64 CAEVGIKSFDVGLPEEVSEADLISK-VHELNSNPDVHGILVQLPLPKHINEEHILGAISI 122
Query: 118 EKDVEGLNHVYYQNMYHNIRYLDDQKQLKSILPCTPLAVVKILEYLKVYNNLLPEGNRLY 177
+KDV+G + + NI L + + LPCTP +++L G ++
Sbjct: 123 DKDVDGFHPL-------NIGKLAMKGREPLFLPCTPKGCLELLAR---------SGVKIK 166
Query: 178 GKKCVVVNRSEIVGRPLAALLANDGADVFSVDINNIQKFTRGQGLKFTKHHVEDLGAYSE 237
G++ VVV RS IVG P++ LL A V +V H +D E
Sbjct: 167 GQRAVVVGRSNIVGLPVSLLLLKADATVTTV-----------------HSHTKD----PE 205
Query: 238 ELLKECCKDADVIITGVPGEKYKFPTDYIKEGAICINFSSEKNFDESVKSKASLY----- 292
+++E AD++I G+ + ++IK GA I+ + D S KS L
Sbjct: 206 AIIRE----ADIVIAAC-GQAHMIKGNWIKPGAAVIDVGTNAVSDPSKKSGYRLVGDVDF 260
Query: 293 ------------VP-STGKVTISMLLRN 307
VP G +T++MLLRN
Sbjct: 261 AEASKVAGFITPVPGGVGPMTVAMLLRN 288
>Hs5729935 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 344
Score = 72.0 bits (175), Expect = 1e-12
Identities = 84/335 (25%), Positives = 136/335 (40%), Gaps = 60/335 (17%)
Query: 10 ILASSISKTYVQEITGKVESLRNIRPDGPLLVGFLANNDPAAEMYASWTQKTCESMGFRY 69
I +++ QE+ +VE P L L +PA+ Y + +G
Sbjct: 34 ISGRKLAQQIKQEVRQEVEEWVASGNKRPHLSVILVGENPASHSYVLNKTRAAAVVGINS 93
Query: 70 ELRRIEDRDFLEEAIIDA----NQDDSVDGIMIYFPVFGNAQDQYLQQVVSREKDVEGLN 125
E ++ EE +++ N DD+VDG+++ P+ + ++ + VS +KDV+G +
Sbjct: 94 ETI-MKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERRICNAVSPDKDVDGFH 152
Query: 126 HVYYQNMYHNIRYLDDQKQLKSILPCTPLAVVKILEYLKVYNNLLPEGNRLYGKKCVVVN 185
+ M LD S+LP TP V +I++ G GK VV
Sbjct: 153 VINVGRM-----CLDQY----SMLPATPWGVWEIIK---------RTGIPTLGKNVVVAG 194
Query: 186 RSEIVGRPLAALLANDGADVFSVDINNIQKFTRGQGLKFTKHHVEDLGAYSEELLKECCK 245
RS+ VG P+A LL DGA + G H +E LK+
Sbjct: 195 RSKNVGMPIAMLLHTDGA----------HERPGGDATVTISHRYT-----PKEQLKKHTI 239
Query: 246 DADVIIT--GVPGEKYKFPTDYIKEGAICINFSSEKNFD--------------ESVKSKA 289
AD++I+ G+P D IKEGA I+ + D E V+ KA
Sbjct: 240 LADIVISAAGIPN---LITADMIKEGAAVIDVGINRVHDPVTAKPKLVGDVDFEGVRQKA 296
Query: 290 SLYVP---STGKVTISMLLRNMLRLVENKQKLQNR 321
P G +T++ML++N + + +L+ R
Sbjct: 297 GYITPVPGGVGPMTVAMLMKNTIIAAKKVLRLEER 331
>SPBC839.16 [H] KOG4230 C1-tetrahydrofolate synthase
Length = 937
Score = 69.3 bits (168), Expect = 7e-12
Identities = 66/283 (23%), Positives = 116/283 (40%), Gaps = 64/283 (22%)
Query: 61 TCESMGFRYELRRIEDRDFLEEAIIDANQDDSVDGIMIYFPVFGNAQDQYLQQVVSREKD 120
+CE + F ++ + L AI N+D +V GI++ P+ + +Q + + V+ EKD
Sbjct: 64 SCEHVNFPEDITEYD----LLLAIKGFNEDPTVHGIIVQLPLPAHINEQIITEAVAPEKD 119
Query: 121 VEGLNHVYYQNMYHNIRYLDDQKQLKSILPCTPLAVVKILEYLKVYNNLLPEGNRLYGKK 180
V+G N+ L ++ CTP ++ IL++ G + GK
Sbjct: 120 VDGFCET-------NLGKLTKREGQPLFTACTPKGIMCILKHY---------GINVQGKH 163
Query: 181 CVVVNRSEIVGRPLAALLANDGADVFSVDINNIQKFTRGQGLKFTKHHVEDLGAYSEELL 240
VV+ RS IVGRP++ LL A V L E +
Sbjct: 164 AVVIGRSNIVGRPMSILLEKANATV-------------------------TLCHSKTESI 198
Query: 241 KECCKDADVIITGVPGEKYKFPTDYIKEGAICINFSSEKNFD---------------ESV 285
+ + AD+++ + G + D++K+G + I+ D E+
Sbjct: 199 ADIVRTADIVVAAI-GIPHFVKADWLKKGVVAIDVGINSIPDATKKSGYRLTGDIDFENA 257
Query: 286 KSKASLYVP---STGKVTISMLLRNMLRLVENKQKLQNRSAST 325
K AS P S G +T++MLL+N++ +K+ + T
Sbjct: 258 KEVASAITPVPGSVGPMTVAMLLQNVVESAVRFRKMSRKRKPT 300
>CE25047 [H] KOG4230 C1-tetrahydrofolate synthase
Length = 303
Score = 69.3 bits (168), Expect = 7e-12
Identities = 78/324 (24%), Positives = 135/324 (41%), Gaps = 64/324 (19%)
Query: 16 SKTYVQEITGKVESLRNIRPDGPLLVGFL-ANNDPAAEMYASWTQKTCESMGFRYELRRI 74
SK + ++ K+ R P+ ++ + N + +Y + K + +G +L ++
Sbjct: 12 SKKVLHDVGQKIAKTREHHPNFHAVLAIVQVGNRSDSNVYINSKLKKAKEIGADGKLIKL 71
Query: 75 ED---RDFLEEAIIDANQDDSVDGIMIYFPVFGNAQ---DQYLQQVVSREKDVEGLNHVY 128
D + L+ I+ N D+ +DGI+I P+ + D + ++ KDV+GL +
Sbjct: 72 PDTITQGDLKREIMALNHDNEIDGIIIQLPLDCKHEIDADSVID-LIDPLKDVDGLTRI- 129
Query: 129 YQNMYHNIRYLDDQKQLKSILPCTPLAVVKILEYLKVYNNLLPEGNRLYGKKCVVVNRSE 188
N L + ++I PCTP + +++ + N + GK+ VV+ RS+
Sbjct: 130 ------NAGRLARGELQRTIFPCTPFGCLYLVQ------QATGDANFVSGKEVVVLGRSK 177
Query: 189 IVGRPLAALLANDGADVFSVDINNIQKFTRGQGLKFTKHHVEDLGAYSE-ELLKECCKDA 247
IVG P AAL F HH +S+ LKE C A
Sbjct: 178 IVGSPAAAL--------------------------FLWHHGTVTICHSKTPNLKEKCLRA 211
Query: 248 DVIITGVPGEKYKFPTDYIKEGAIC----INFSSEKNF--------DESVKSKASLYVP- 294
D++I + G K+ D+IK GA IN + N E+ K A P
Sbjct: 212 DILIVAI-GRKHFVKADWIKPGAFVIDCGINVGDDPNSRKIYGDVDTEAAKEVAGFLTPV 270
Query: 295 --STGKVTISMLLRNMLRLVENKQ 316
G +T++ML+RN + ++
Sbjct: 271 PGGVGPMTVAMLIRNTFEQAKRRR 294
>YGR204w [H] KOG4230 C1-tetrahydrofolate synthase
Length = 946
Score = 62.0 bits (149), Expect = 1e-09
Identities = 76/349 (21%), Positives = 138/349 (38%), Gaps = 80/349 (22%)
Query: 7 GRTILASSISKTYVQEITGKVESLRNIRPD-GPLLVGFLANNDPAAEMYASWTQKTCESM 65
G+ + + ++ + I +++S++ P P L N P + Y +K E
Sbjct: 3 GQVLDGKACAQQFRSNIANEIKSIQGHVPGFAPNLAIIQVGNRPDSATYVRMKRKAAEEA 62
Query: 66 GFRYELRRIEDR--DFLEEAIIDA-NQDDSVDGIMIYFPVFGNAQDQYLQQVVSREKDVE 122
G +++ +F +D N+D GI++ P+ + + + V EKDV+
Sbjct: 63 GIVANFIHLDESATEFEVLRYVDQLNEDPHTHGIIVQLPLPAHLDEDRITSRVLAEKDVD 122
Query: 123 GLNHVYYQNMYHNIRYLDDQKQLKSILPCTPLAVVKILEYLKVYNNLLPEGNRLYGKKCV 182
G NI L+ + LPCTP ++++L V + G + V
Sbjct: 123 GFGPT-------NIGELNKKNGHPFFLPCTPKGIIELLHKANV---------TIEGSRSV 166
Query: 183 VVNRSEIVGRPLAALLANDGADVFSVDINNIQKFTRGQGLKFTKHHVEDLGAYSEELLKE 242
V+ RS+IVG P+A LL + + V T D+ +Y
Sbjct: 167 VIGRSDIVGSPVAELLKSLNSTV-----------------TITHSKTRDIASY------- 202
Query: 243 CCKDADVIITG------VPGEKYK---------------FPTDYI-----KEGAICINFS 276
DAD+++ V GE +K T+Y+ K G C+
Sbjct: 203 -LHDADIVVVAIGQPEFVKGEWFKPRDGTSSDKKTVVIDVGTNYVADPSKKSGFKCV--- 258
Query: 277 SEKNFDESVKSKASLYVP---STGKVTISMLLRNMLRLVENKQKLQNRS 322
+ F+E++K L P G +T++ML++N L+ K++++ S
Sbjct: 259 GDVEFNEAIK-YVHLITPVPGGVGPMTVAMLMQN--TLIAAKRQMEESS 304
>At4g00600 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 310
Score = 62.0 bits (149), Expect = 1e-09
Identities = 61/240 (25%), Positives = 97/240 (40%), Gaps = 60/240 (25%)
Query: 88 NQDDSVDGIMIYFPVFGNAQDQYLQQVVSREKDVEGLNHVYYQNMYHNIRYLDDQKQLKS 147
N D SV G+++ P+ + +Q + VS EKDV+G + + NI L + +
Sbjct: 103 NDDPSVHGVLVQLPLPSHMDEQNILNAVSIEKDVDGFHPL-------NIGRLAMRGREPL 155
Query: 148 ILPCTPLAVVKILEYLKVYNNLLPEGNRLYGKKCVVVNRSEIVGRPLAALLANDGADVFS 207
+PCTP +++L + GK+ VV+ RS IVG P A LL + A V
Sbjct: 156 FVPCTPKGCIELLHRYNI---------EFKGKRAVVIGRSNIVGMPAALLLQKEDATVSI 206
Query: 208 VDINNIQKFTRGQGLKFTKHHVEDLGAYSEELLKECCKDADVIITGVPGEKYKFPTDYIK 267
+ + +E + AD++I+ V G+ +IK
Sbjct: 207 IHSRTMNP-------------------------EELTRQADILISAV-GKPNMVRGSWIK 240
Query: 268 EGAICINFSSEKNFDESVKSKASL-----YVPST-------------GKVTISMLLRNML 309
GA+ I+ + D S L YV ++ G +TI+MLL N L
Sbjct: 241 PGAVLIDVGIKPVEDPSAAGGERLVGDICYVEASKIASAITPVPGDVGPMTIAMLLSNTL 300
>At2g38660 [H] KOG0089 Methylenetetrahydrofolate
dehydrogenase/methylenetetrahydrofolate cyclohydrolase
Length = 350
Score = 62.0 bits (149), Expect = 1e-09
Identities = 80/316 (25%), Positives = 126/316 (39%), Gaps = 39/316 (12%)
Query: 3 TEKPGRTILASSISKTYVQEITGKVESLRNIRPDGPLLVGFLANNDPAAEMYASWTQKTC 62
TE+ I + I++ +I +V ++ P L L ++ Y K C
Sbjct: 57 TEQKTVVIDGNVIAEEIRTKIISEVGKMKKAVGKVPGLAVVLVGEQRDSQTYVRNKIKAC 116
Query: 63 ESMGFRYELRRIEDRDFLEEAIIDA----NQDDSVDGIMIYFPVFGNAQDQYLQQVVSRE 118
E G + L + + D E II N+D S+ GI++ P+ + + + +V E
Sbjct: 117 EETGIKSVLAELPE-DCTEGQIISVLRKFNEDTSIHGILVQLPLPQHLNESKILNMVRLE 175
Query: 119 KDVEGLNHVYYQNMYHNIRYLDDQKQLKSILPCTPLAVVKILEYLKVYNNLLPEGNRLYG 178
KDV+G + + N+ L + + + CTP V++L + G + G
Sbjct: 176 KDVDGFHPL-------NVGNLAMRGREPLFVSCTPKGCVELL---------IRTGVEIAG 219
Query: 179 KKCVVVNRSEIVGRPLAALLANDGADVFSVD--INNIQKFTRGQGLKFTKHHVEDLGAYS 236
K VV+ RS IVG P++ LL A V +V + + TR + + +L S
Sbjct: 220 KNAVVIGRSNIVGLPMSLLLQRHDATVSTVHAFTKDPEHITRKADIVIAAAGIPNLVRGS 279
Query: 237 EELLKECCKDADVIITGVPGEKYKFPTDYIKEGAICINFSSEKNFDESVKSKASLYVP-- 294
LK DV T V +F Y G +C E AS P
Sbjct: 280 --WLKPGAVVIDVGTTPVEDSSCEF--GYRLVGDVCY---------EEALGVASAITPVP 326
Query: 295 -STGKVTISMLLRNML 309
G +TI+MLL N L
Sbjct: 327 GGVGPMTITMLLCNTL 342
>SPBC2G2.08 [H] KOG4230 C1-tetrahydrofolate synthase
Length = 969
Score = 51.6 bits (122), Expect = 2e-06
Identities = 45/200 (22%), Positives = 84/200 (41%), Gaps = 24/200 (12%)
Query: 10 ILASSISKTYVQEITGKVESLRNIRPDGPLLVGFLANNDPAAEMYASWTQKTCESMGFRY 69
+LA + ++ +E+ RN +P L + +Y +K +G +
Sbjct: 40 LLARKLQRSVAEEVQALKAKDRNFKP---ALAIVQVGKREDSNVYVRMKEKAARLVGIDF 96
Query: 70 EL----RRIEDRDFLEEAIIDANQDDSVDGIMIYFPVFGNAQDQYLQQVVSREKDVEGLN 125
+ I+ L E + N D +V G+++ P+ + ++ + + ++ KDV+G
Sbjct: 97 KYCPFPETIQMPALLHE-LKKLNDDHTVHGVLVQLPLPKHLNERTVTESITPPKDVDGFG 155
Query: 126 HVYYQNMYHNIRYLDDQKQLKSILPCTPLAVVKILEYLKVYNNLLPEGNRLYGKKCVVVN 185
NI L PCTP ++++L+ K+ + G VV+
Sbjct: 156 AF-------NIGLLAKNDATPIHYPCTPKGIMELLKDNKI---------SVAGLNAVVLG 199
Query: 186 RSEIVGRPLAALLANDGADV 205
RS+IVG P++ LL D A V
Sbjct: 200 RSDIVGNPISYLLRKDNATV 219
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.136 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,396,931
Number of Sequences: 60738
Number of extensions: 862934
Number of successful extensions: 2145
Number of sequences better than 1.0e-05: 13
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 2122
Number of HSP's gapped (non-prelim): 13
length of query: 326
length of database: 30,389,216
effective HSP length: 107
effective length of query: 219
effective length of database: 23,890,250
effective search space: 5231964750
effective search space used: 5231964750
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)