ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI2087 good R KOG1947 General function prediction only Leucine rich repeat proteins, some proteins contain F-box
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI2087 711231 712955 575
(575 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJR052w [R] KOG1947 Leucine rich repeat proteins some proteins c... 437 e-122
SPCC330.02 [R] KOG1947 Leucine rich repeat proteins some protein... 162 1e-39
7303582 [R] KOG4341 F-box protein containing LRR 60 9e-09
At5g23340 [R] KOG1947 Leucine rich repeat proteins some proteins... 58 4e-08
Hs16306582 [R] KOG4341 F-box protein containing LRR 52 2e-06
Hs14249620 [R] KOG4341 F-box protein containing LRR 52 3e-06
>YJR052w [R] KOG1947 Leucine rich repeat proteins some proteins contain
F-box
Length = 565
Score = 437 bits (1123), Expect = e-122
Identities = 249/583 (42%), Positives = 334/583 (56%), Gaps = 26/583 (4%)
Query: 1 MYRARSRRNNDGSG-VRGPNSALTQFLKEQGISAENIRDRWLEAQKKEAEHPQETQKDDS 59
MYR+R+R G V+GPNSALTQFL+E+GISAENI+ +W + Q K+ E + +K +
Sbjct: 1 MYRSRNRPKRGGENEVKGPNSALTQFLREEGISAENIKQKWYQRQSKKQEDATDEKKGKA 60
Query: 60 ETESDEDLKAGVAAKKRSRKPRAVKKQXXXXXXXXXXXXXXXXXXXXXYKAEARLRRLRG 119
E D+ A ++ R V+ + +AR++ +
Sbjct: 61 E---DDSFTAEIS--------RVVEDEEIDEIGTGSGTETERAQVSY----DARMKLVPA 105
Query: 120 AXXXXXXXXXXXXXXXXRKKRMLTLTNGKKTSPQLAKARHQQKKTRRKKAANXXXXXXXX 179
+ K AK Q ++ +RK+AA+
Sbjct: 106 DSDEEEYETSHISDTPVSLSSANDRESLTKKRQNTAKII-QNRRRKRKRAADLLDRRVNK 164
Query: 180 XXXXQDICIKVIGKSIVEYNAQS------MVEHIRDTLGGVSLENLNKLAQALTKNRALN 233
Q +CI I ++I ++ ++ + +RD LGGVS NLN LA+AL+KNRALN
Sbjct: 165 VSSLQSLCITKISENISKWQKEADESSKLVFNKLRDVLGGVSTANLNNLAKALSKNRALN 224
Query: 234 DDXXXXXXXXXXXXXXFYDCSKLSYEGYKRLAIFSPHLESLSLQMCGQLNNEALLYLAEK 293
D F DCSK+S++GYK LAIFSPHL LSLQMCGQLN+E+LLY+AEK
Sbjct: 225 DHTLQLFLKTDLKRLTFSDCSKISFDGYKTLAIFSPHLTELSLQMCGQLNHESLLYIAEK 284
Query: 294 LPNLKELYLDGPFLINEDTWVQFFELLKGRLTAFHVSNTHRFNDNALSAMLRNCGSSLES 353
LPNLK L LDGPFLINEDTW +FF ++KGRL FH+SNTHRF D +LS +L NCGS+L S
Sbjct: 285 LPNLKSLNLDGPFLINEDTWEKFFVIMKGRLEEFHISNTHRFTDKSLSNLLINCGSTLVS 344
Query: 354 LGLATLDTVTNYALLPQYLNSPNFHTLILEEPSRETDINDEVIISLLGSIGQNLRKLSLN 413
LGL+ LD+++NYALLPQYL + FH+L +E P E D+NDE+II+LLG IG+ LRKL LN
Sbjct: 345 LGLSRLDSISNYALLPQYLVNDEFHSLCIEYPFNEEDVNDEIIINLLGQIGRTLRKLVLN 404
Query: 414 RCTGLTDSCIINGFLPFLSNSGQSILTDLELEELDQITTDGIVLLFSSLQFPLLKRCSLK 473
C LTDS IING F+ + L L LEE DQITTD + FS ++ L CS +
Sbjct: 405 GCIDLTDSMIINGLTAFIPE--KCPLEVLSLEESDQITTDSLSYFFSKVELNNLIECSFR 462
Query: 474 RCF-XXXXXXXXXXXXXXXXXXXXXXXXXXKSLTSKTFTFMACPNLKQLNIGFVHCVDNE 532
RC K LT + F +ACPNL L++GFV CVD+
Sbjct: 463 RCLQLGDMAIIELLLNGARDSLRSLNLNSLKELTKEAFVALACPNLTYLDLGFVRCVDDS 522
Query: 533 IVEKISKDNEKLTIIEVYGDNQVTGKCSPRNGVSIIGRQSDSI 575
+++ + + N LT+I+V+GDN VT K + R G+++IGRQSDSI
Sbjct: 523 VIQMLGEQNPNLTVIDVFGDNLVTEKATMRPGLTLIGRQSDSI 565
>SPCC330.02 [R] KOG1947 Leucine rich repeat proteins some proteins contain
F-box
Length = 563
Score = 162 bits (410), Expect = 1e-39
Identities = 139/582 (23%), Positives = 242/582 (40%), Gaps = 42/582 (7%)
Query: 10 NDGSGVRGPNSALTQFLKEQGISAENI------RDRWLEAQKKEAEHPQETQKDDSETES 63
+ GS VRGPNSALT+FL+ QGI+A + R A + + Q S E+
Sbjct: 2 SSGSRVRGPNSALTEFLRSQGINASALGRARPPRQSEESAGQSTGTESEVIQTPTSVEEN 61
Query: 64 DEDLKAGVAA-------KKRSRKPRAVKKQXXXXXXXXXXXXXXXXXXXXXYKAEARLRR 116
+ED + K+R+ + + KK+ YKA +
Sbjct: 62 NEDENSMSTTTIEIPVVKRRNLRNQKKKKKTDEEAEDNEDTFSMNSRAGFSYKAREHTGK 121
Query: 117 LRGAXXXXXXXXXXXXXXXXRKKR---MLTLTNGKKTSPQLAKARHQQKKTRRKKAANXX 173
L ++ + G + ++K RKK A
Sbjct: 122 LDFCAHCNCRFTITPYSKYSNSEKGWLCYPCSRGAEDRSVPELRTRKRKALTRKKVAAAT 181
Query: 174 XXXXXXXXXXQDICIKVIGKSIVEYNAQSMVEHIRDTLGGVSLENLNKLAQALTKNRALN 233
QD+CI+VI + I + A G + N++K++Q ++KNR+LN
Sbjct: 182 MDEEISVPKLQDLCIRVIAEYINDIEA----------FGDIGQVNMDKISQIISKNRSLN 231
Query: 234 DDXXXXXXXXXXXXXXFYDCSKLSYEGYKRLAIFSPHLESLSLQMCGQLNNEALLYLAEK 293
D YDCSK++ + ++A + P+L++L L CGQ+ ++ L + A+
Sbjct: 232 DTTVKLFLSGGQTELKLYDCSKITADSLFQIAQYCPNLQTLHLTYCGQMQDQVLHFYADH 291
Query: 294 LPNLKELYLDGPFLINEDTWVQFFELLKGRLTAFHVSNTHRFNDNALSAMLRNCGSSLES 353
L L ++ G FL++ W+ FF+ +L + +++T R + + ++A++ +C +L S
Sbjct: 292 LTELTDVSFQGAFLVSSSEWINFFKKRGSKLISLELTDTARIHVSVINAIV-DCCPNLIS 350
Query: 354 LGLATLDTVTNYALLPQYLNSPNFHTLILEEPSRETDINDEVIISLLGSIGQNLRKLSLN 413
L L+ + + + + N +L +E P IND I+ +L IG L LSL+
Sbjct: 351 LNLSRIFYLDD-ECVRLLAGCRNLVSLKIESPG--GIINDGSILDVLNQIGSGLHTLSLS 407
Query: 414 RCTGLTDSCIINGFLPFLSNSGQSILTDLELEELDQITTDGIVLLFSSLQFPL-LKRCSL 472
CT LTD + G P L L L L+ +T D ++F + L+ SL
Sbjct: 408 GCTKLTDEVLKQGIGPCCGR-----LKHLNLSGLELLTDDEASIVFGEWKIQSGLETLSL 462
Query: 473 KRCFXXXXXXXXXXXXXXXXXXXXXXXXXXKSLTSKTFTFMA---CPNLKQLNIGFVHCV 529
+RC +T + ++ P LK L++ ++ +
Sbjct: 463 RRCLSLGDKTVRAVLVNSGHTLRTLDLNGMSFVTDEALQYIVNFPLPMLKALDVSWIRGM 522
Query: 530 DNEIVEKISKDNEKLTIIEVYGDNQVTGKCSPRNGVSIIGRQ 571
++++V L + V+GDN V P N + +IGR+
Sbjct: 523 NDKLVCDFESKKPTLEKLLVWGDNHV---LMPSNRLLLIGRE 561
>7303582 [R] KOG4341 F-box protein containing LRR
Length = 464
Score = 60.1 bits (144), Expect = 9e-09
Identities = 56/224 (25%), Positives = 100/224 (44%), Gaps = 14/224 (6%)
Query: 252 DCSKLSYEGYKRLAIFSPHLESLSLQMCGQLNNEALLYLAEKLPNLKELYLDGPFLINED 311
DC K++ + ++ + L +++L C + + +L YL++ PNL E+ + LI+E+
Sbjct: 154 DCKKITDISTQSISRYCSKLTAINLHSCSNITDNSLKYLSDGCPNLMEINVSWCHLISEN 213
Query: 312 TWVQFFELLKGRLTAFHVSNTHRFNDNALSAMLRNCGSSLESLGLATLDTVTNYALLPQY 371
V+ +L F + NDNA+ + + C L L L + +T+T+ ++
Sbjct: 214 -GVEALARGCVKLRKFSSKGCKQINDNAIMCLAKYC-PDLMVLNLHSCETITDSSIRQLA 271
Query: 372 LNSPNFHTLILEEPSRETDINDEVIISLLGSIGQNLRKLSLNRCTGLTDSCIINGFLPFL 431
N L + S+ D+ D ++S L L L ++ C TD GF
Sbjct: 272 ANCHKLQKLCV---SKCADLTDLTLLS-LSQHNHLLNTLEVSGCRNFTDI----GFQALG 323
Query: 432 SNSGQSILTDLELEELDQITTDGIVLLFSSLQFPLLKRCSLKRC 475
N L ++LEE QIT + L + P L++ +L C
Sbjct: 324 RNC--KYLERMDLEECSQIT--DLTLAHLATGCPSLEKLTLSHC 363
>At5g23340 [R] KOG1947 Leucine rich repeat proteins some proteins contain
F-box
Length = 405
Score = 57.8 bits (138), Expect = 4e-08
Identities = 48/173 (27%), Positives = 84/173 (47%), Gaps = 8/173 (4%)
Query: 253 CSKLSYEGYKRLAIFSPHLESLSLQMCGQLNNEALLYLAEKLPNLKELYLDGPFLINEDT 312
C KLS +G +A L +L L C + +E+L L+E+ +L+ L L G I +
Sbjct: 134 CRKLSDKGLSAVAEGCHDLRALHLAGCRFITDESLKSLSERCRDLEALGLQGCTNITDSG 193
Query: 313 WVQFFELLKG--RLTAFHVSNTHRFNDNALSAMLRNCGSSLESLGLATLDTVTNYALLPQ 370
+L+KG ++ + ++ D +S++ + C SSL++L L V N ++
Sbjct: 194 LA---DLVKGCRKIKSLDINKCSNVGDAGVSSVAKACASSLKTLKLLDCYKVGNESISSL 250
Query: 371 YLNSPNFHTLILEEPSRETDINDEVIISLLGSIGQNLRKLSLNRCTGLTDSCI 423
N TLI+ DI+DE I+ L S +L+ L ++ C ++DS +
Sbjct: 251 AQFCKNLETLII---GGCRDISDESIMLLADSCKDSLKNLRMDWCLNISDSSL 300
>Hs16306582 [R] KOG4341 F-box protein containing LRR
Length = 423
Score = 52.4 bits (124), Expect = 2e-06
Identities = 55/205 (26%), Positives = 85/205 (40%), Gaps = 14/205 (6%)
Query: 253 CSKLSYEGYKRLAIFSPHLESLSLQMCGQLNNEALLYLAEKLPNLKELYLDGPFLINEDT 312
C++L E K + + L SL+LQ C ++ +E ++ + L+ L L G + D
Sbjct: 192 CTQLEDEALKHIQNYCHELVSLNLQSCSRITDEGVVQICRGCHRLQALCLSGCSNLT-DA 250
Query: 313 WVQFFELLKGRLTAFHVSNTHRFNDNALSAMLRNCGSSLESLGLATLDTVTNYALLPQYL 372
+ L RL + D + + RNC LE + L +T+ L+ +
Sbjct: 251 SLTALGLNCPRLQILEAARCSHLTDAGFTLLARNC-HELEKMDLEECILITDSTLIQLSI 309
Query: 373 NSPNFHTLILEEPSRETDINDEVIISLLGSI--GQNLRKLSLNRCTGLTDSCIINGFLPF 430
+ P L L S I D+ I+ L S + LR L L+ C +TD L
Sbjct: 310 HCPKLQALSL---SHCELITDDGILHLSNSTCGHERLRVLELDNCLLITDVA-----LEH 361
Query: 431 LSNSGQSILTDLELEELDQITTDGI 455
L N L LEL + Q+T GI
Sbjct: 362 LENCRG--LERLELYDCQQVTRAGI 384
>Hs14249620 [R] KOG4341 F-box protein containing LRR
Length = 422
Score = 51.6 bits (122), Expect = 3e-06
Identities = 50/203 (24%), Positives = 81/203 (39%), Gaps = 12/203 (5%)
Query: 253 CSKLSYEGYKRLAIFSPHLESLSLQMCGQLNNEALLYLAEKLPNLKELYLDGPFLINEDT 312
C+ ++ K L+ P LE L++ C Q+ + + L LK L+L G + ED
Sbjct: 139 CTSITNMSLKALSEGCPLLEQLNISWCDQVTKDGIQALVRGCGGLKALFLKGCTQL-EDE 197
Query: 313 WVQFFELLKGRLTAFHVSNTHRFNDNALSAMLRNCGSSLESLGLATLDTVTNYALLPQYL 372
+++ L ++ + D L + R C L+SL + +T+ L
Sbjct: 198 ALKYIGAHCPELVTLNLQTCLQITDEGLITICRGC-HKLQSLCASGCSNITDAILNALGQ 256
Query: 373 NSPNFHTLILEEPSRETDINDEVIISLLGSIGQNLRKLSLNRCTGLTDSCIINGFLPFLS 432
N P L + S+ TD+ + L L K+ L C +TDS +I
Sbjct: 257 NCPRLRILEVARCSQLTDVG----FTTLARNCHELEKMDLEECVQITDSTLIQ------L 306
Query: 433 NSGQSILTDLELEELDQITTDGI 455
+ L L L + IT DGI
Sbjct: 307 SIHCPRLQVLSLSHCELITDDGI 329
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.133 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,551,197
Number of Sequences: 60738
Number of extensions: 1019357
Number of successful extensions: 5047
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 5005
Number of HSP's gapped (non-prelim): 31
length of query: 575
length of database: 30,389,216
effective HSP length: 112
effective length of query: 463
effective length of database: 23,586,560
effective search space: 10920577280
effective search space used: 10920577280
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)