ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI2225 good I KOG3964 Lipid transport and metabolism Phosphatidylglycerolphosphate synthase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI2225 754857 756476 540
(540 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YCL004w [I] KOG3964 Phosphatidylglycerolphosphate synthase 545 e-155
SPBP18G5.02 [I] KOG3964 Phosphatidylglycerolphosphate synthase 322 7e-88
7300436 [I] KOG3964 Phosphatidylglycerolphosphate synthase 231 3e-60
Hs21314624 [I] KOG3964 Phosphatidylglycerolphosphate synthase 211 2e-54
CE25321 [I] KOG3964 Phosphatidylglycerolphosphate synthase 105 2e-22
HsM13259370 [I] KOG3964 Phosphatidylglycerolphosphate synthase 104 4e-22
CE27264 [I] KOG3964 Phosphatidylglycerolphosphate synthase 82 2e-15
>YCL004w [I] KOG3964 Phosphatidylglycerolphosphate synthase
Length = 521
Score = 545 bits (1405), Expect = e-155
Identities = 268/486 (55%), Positives = 361/486 (74%), Gaps = 9/486 (1%)
Query: 61 GNGFLDMVKEKLSFLNTKVYLRQGEVEILDHPVQFYSTLKNKISAAQNRVFMASLYLGKT 120
GN + K L T + + E++I++ P QFY LK KI +QNR+F+ASLYLGK+
Sbjct: 39 GNYLNTITKSLQQNLQTCFHFQAKEIDIIESPSQFYDLLKTKILNSQNRIFIASLYLGKS 98
Query: 121 ENDLINCISDSLKKNKDLKVYFMVDGLRGTRETPNKCSASLLAELEKEFG-ERVEIRLYR 179
E +L++CIS +L KN LKV F++DGLRGTRE P+ CSA+LL+ L ++G ERV+ RLY+
Sbjct: 99 ETELVDCISQALTKNPKLKVSFLLDGLRGTRELPSACSATLLSSLVAKYGSERVDCRLYK 158
Query: 180 TPEFVGWK-TLIPKRFNEGLGLQHMKIYGVDEEVILSGANLSNDYFTNRQDRYYVFKNKF 238
TP + GWK L+PKRFNEGLGLQHMKIYG D EVILSGANLSNDYFTNRQDRYY+FK++
Sbjct: 159 TPAYHGWKKVLVPKRFNEGLGLQHMKIYGFDNEVILSGANLSNDYFTNRQDRYYLFKSRN 218
Query: 239 LSDYYFKVHQLVSKISYRVRHANNEQKYHLEWPESNVAVEPLKDKHKFIEKCSSIFTDFL 298
S+YYFK+HQL+S SY++ + ++ WP+SN VEP K+K F+ + S + FL
Sbjct: 219 FSNYYFKLHQLISSFSYQIIKPMVDGSINIIWPDSNPTVEPTKNKRLFLREASQLLDGFL 278
Query: 299 RGPSSMGNAHLIKDFSQYPTIVYPVSQFTPLFQPHHDVSTEKPGVLSILSCLSNPMSRWT 358
+ S + I Q+ T+VYP+SQFTPLF ++D STEK +LS+LS +++ WT
Sbjct: 279 K---SSKQSLPITAVGQFSTLVYPISQFTPLFPKYNDKSTEKRTILSLLSTITSNAISWT 335
Query: 359 FTAGYFNMLPEIKQRLLDSPSIQGQVITASPYANGFFQSPGVSGHLPDAYLYLSQGFLKD 418
FTAGYFN+LP+IK +LL +P + VITASP+ANGF+QS GVS +LP AYLYLS+ FL+D
Sbjct: 336 FTAGYFNILPDIKAKLLATPVAEANVITASPFANGFYQSKGVSSNLPGAYLYLSKKFLQD 395
Query: 419 VHRHGKENHIKLREWKHGIVNQANGWSYHAKGLWLS----EPGNDKRPCLTIVGSSNYTR 474
V R+ +++ I LREW+ G+VN+ NGWSYHAKG+WLS N+ +P +T++GSSNYTR
Sbjct: 396 VCRYRQDHAITLREWQRGVVNKPNGWSYHAKGIWLSARDKNDANNWKPFITVIGSSNYTR 455
Query: 475 RAYSLDIETNAIVLTKDEQLKTAMQNEIDNLLKNTKEVTLGSFEEDPDRHISTGVKLATK 534
RAYSLD+E+NA+++T+DE+L+ M+ E+DNLL+ TK VTL F+ DP+RH+ TGVK+AT
Sbjct: 456 RAYSLDLESNALIITRDEELRKKMKAELDNLLQYTKPVTLEDFQSDPERHVGTGVKIATS 515
Query: 535 ILGNRL 540
ILG +L
Sbjct: 516 ILGKKL 521
>SPBP18G5.02 [I] KOG3964 Phosphatidylglycerolphosphate synthase
Length = 534
Score = 322 bits (826), Expect = 7e-88
Identities = 202/532 (37%), Positives = 299/532 (55%), Gaps = 83/532 (15%)
Query: 58 GQDGNGFLDMVKEKLSFLNTKVYLRQGEVEILDHPVQFYSTLKNKISAAQNRVFMASLYL 117
G + N F+++ + ++ + K Y+ +++I+ P +FY LK I AQ R+F+++LY+
Sbjct: 4 GIEKNIFVNL-ESQIDGVCPKFYVNVDDIDIIHEPPEFYQRLKKLIKKAQKRIFLSTLYI 62
Query: 118 GKTENDLINCISDSLKKNKDLKVYFMVDGLRGTRETPNKCSASLLAELEKEFGERVEIRL 177
GK E +LINC+S++L N L V+ + D LR TRE+P CSASLL +L+K+F +R EI+L
Sbjct: 63 GKEERELINCLSNALSNNPSLHVHILADQLRCTRESPGCCSASLLMQLKKKFPDRCEIKL 122
Query: 178 YRTPEFVGW-KTLIPKRFNEGLGLQHMKIYGVDEEVILSGANLSNDYFTNRQDRYYVFKN 236
Y TP G K L+P RFNEG GLQHMKIYG D+ +I+SGANLS DYFTNR+DRYY+F +
Sbjct: 123 YHTPNLRGLRKQLVPHRFNEGWGLQHMKIYGADDNLIISGANLSRDYFTNRKDRYYLFSD 182
Query: 237 KFLSDYYFKVHQLVSKISYR-VRHANNEQ----------KYHLEWPESNVAVEPLKDKHK 285
K L+D++FK H L S++S+ + H ++ + L+W +N PL + +
Sbjct: 183 KGLADFFFKTHFLFSQLSFECIPHLSDSSIQLSSTSPVIPFTLKW--NNSCPNPLTNPQE 240
Query: 286 FIEKCSSIFTDFLRGP-------------SSMGNAHLI--------KDFSQYP--TIVYP 322
F S+ L+G SS+ + LI K F +Y IVYP
Sbjct: 241 FRVAASAKIQQLLQGNREKFLSRNPSKPLSSVYGSELINQAGDDNNKPFHKYEESAIVYP 300
Query: 323 VSQFTPLFQPHHDVSTEKPGVLSIL-SCLSNPMSRWTFTAGYFNMLPEIKQRLLDSPSIQ 381
+ Q P+ DV + + VLSI+ + LS WT TAGYFN+ P ++++LL S I
Sbjct: 301 LFQCVPILTS--DVHSTEEKVLSIIGTLLSRKEVNWTLTAGYFNVYPALRKQLLKSEGI- 357
Query: 382 GQVITASPYANGFFQSPGVSGHLPDAYLYLSQGFLKDVHRHGKENHIKLREWKHGIVNQA 441
G+VI AS ANGF++SPG S +P AY Y+++ FLKD + K+ +I + +W Q
Sbjct: 358 GEVIVASQQANGFYRSPGPSKLIPPAYQYIAEQFLKDSRK--KKRNIDVLQW------QN 409
Query: 442 NGWSYHAKG--------------------------------LWLSEPGNDKRPCLTIVGS 469
G +YHAKG WLS + K P LT +GS
Sbjct: 410 KGNTYHAKGKHYSLSFSIINIFWSTALKSYIFEKFTNTILGFWLSTQ-HHKHPFLTTIGS 468
Query: 470 SNYTRRAYSLDIETNAIVLTKDEQLKTAMQNEIDNLLKNTKEVTLGSFEEDP 521
SNYT R+ LD+E+ +V+T++E+LK EI+ + ++TK + E+ P
Sbjct: 469 SNYTSRSQQLDLESTLVVMTQNEKLKRKFSTEIELIKQHTKPMNTCQLEKVP 520
>7300436 [I] KOG3964 Phosphatidylglycerolphosphate synthase
Length = 493
Score = 231 bits (588), Expect = 3e-60
Identities = 156/464 (33%), Positives = 246/464 (52%), Gaps = 33/464 (7%)
Query: 49 QQVRKLKLTGQDGNGFLDMVKEKLSFLNTKVY---LRQGEVEILDHPVQFYSTLKNKISA 105
QQ + TG G L E LS+L+ LR +++++ P FY TL +I
Sbjct: 25 QQAPSVLATGFPGAPAL----ESLSWLHNLAPCFPLRGDQIQVIHEPKHFYETLVQRIGQ 80
Query: 106 AQNRVFMASLYLG--KTENDLINCISDSLKKNKDLKVYFMVDGLRGTRETPNKCSASLLA 163
A+ R+ +ASLYLG + EN ++ + SL++ L++ ++D RGTR T N S ++L
Sbjct: 81 AKRRIVLASLYLGTGQLENAMVQTLRHSLEQQSALRLNVLLDFTRGTRGTLN--SKTMLL 138
Query: 164 ELEKEFGERVEIRLYRTPEFVGW-KTLIPKRFNEGLGLQHMKIYGVDEEVILSGANLSND 222
L ++F +V++ LY TP+ G K L P R+NE LGLQHMK+Y D+ VI+SGANLSND
Sbjct: 139 PLVRDFASQVQLSLYHTPDLRGMTKRLAPPRWNELLGLQHMKVYLFDDAVIISGANLSND 198
Query: 223 YFTNRQDRYYVFKNKFLSDYYFKVHQLVSKISYRVRHANNEQKYHLEWPESNVAVEPLK- 281
YFTNRQDRY + ++K L+D+Y + + V + S V + + H W + P +
Sbjct: 199 YFTNRQDRYILIEDKPLADFYAQFIERVQEFSLAVA-PDASEGLHRNW-----RILPYEG 252
Query: 282 DKHKFIEKCSSIFTDFLRGPSSMGNAHLIKDFSQYPTIVYPVSQFTPLFQPHHDVSTEKP 341
K +FI+ +D ++ + A + Q T ++P+ + + H V T++
Sbjct: 253 TKEQFIQLARKRISDLVQ-ETFQRQARTKEQNPQADTWIFPLLEMGQIGIHHDSVVTKR- 310
Query: 342 GVLSILSCLSNPMSRWTFTAGYFNMLPEIKQRLLDSPSIQGQVITASPYANGFFQSPGVS 401
+ +CLS SR GYFN+ E L Q ++ A P ANGF + G +
Sbjct: 311 ---LLSNCLSG--SRLKLATGYFNLTQEYMDTLTHKCLAQCSILMAHPNANGFQGAKGPA 365
Query: 402 GHLPDAYLYLSQGFLKDVHRHGKENHIKLREWKHGIVNQANGWSYHAKGLWLSEPGNDKR 461
G +P AY +++ F + + R + + + E+ + GW+YHAKGLW P
Sbjct: 366 GGIPAAYTLIAKSFYESLVRRKQNHRVNFFEY------EKPGWTYHAKGLWYYLP-EAIL 418
Query: 462 PCLTIVGSSNYTRRAYSLDIETNAIVLTKDEQLKTAMQNEIDNL 505
P LT++GSSN+ R+ + D+ET ++T ++ L +Q E D L
Sbjct: 419 PNLTLIGSSNFGERSVNRDLETQVCLVTANKDLSQRLQAEADRL 462
>Hs21314624 [I] KOG3964 Phosphatidylglycerolphosphate synthase
Length = 447
Score = 211 bits (537), Expect = 2e-54
Identities = 147/463 (31%), Positives = 248/463 (52%), Gaps = 41/463 (8%)
Query: 99 LKNKISAAQNRVFMASLYLGK--TENDLINCISDSLKKN------KDLKVYFMVDGLRGT 150
+K +I A+ RV MASLYLG E +L++C+ +L+K+ +LKV ++D RG+
Sbjct: 1 MKGQIRVAKRRVVMASLYLGTGPLEQELVDCLESTLEKSLQAKFPSNLKVSILLDFTRGS 60
Query: 151 RETPNKCSASLLAELEKEFGERVEIRLYRTPEFVGW-KTLIPKRFNEGLGLQHMKIYGVD 209
R N S ++L L + F E+V + L+ TP G + LIP+RFNE +GLQH+K+Y D
Sbjct: 61 RGRKN--SRTMLLPLLRRFPEQVRVSLFHTPHLRGLLRLLIPERFNETIGLQHIKVYLFD 118
Query: 210 EEVILSGANLSNDYFTNRQDRYYVFKN-KFLSDYYFKVHQLVSKISYRVRHANNEQKYH- 267
VILSGANLS+ YFTNRQDRY ++ ++D++ ++ V +S +++ + Q
Sbjct: 119 NSVILSGANLSDSYFTNRQDRYVFLQDCAEIADFFTELVDAVGDVSLQLQGDDTVQVVDG 178
Query: 268 LEWPESNVAVEPLKDKHK-FIEKCSSIFT--DFLRGPSSMGNAHLIKDFSQY-------- 316
+ P E K +K ++ +S T L + N+ L ++ +
Sbjct: 179 MVHPYKGDRAEYCKAANKRVMDVINSARTRQQMLHAQTFHSNSLLTQEDAAAAGDRRPAP 238
Query: 317 PTIVYPVSQFTPLFQPHHDVSTEKPGVLSILSCLSNPMSRWTFTAGYFNMLPEIKQRLLD 376
T +YP+ Q P ++ TE L + ++ T GYFN+ +L
Sbjct: 239 DTWIYPLIQMKPFEIQIDEIVTE------TLLTEAERGAKVYLTTGYFNLTQAYMDLVLG 292
Query: 377 SPSIQGQVITASPYANGFFQSPGVSGHLPDAYLYLSQGFLKDVHRHGKENHIKLRE-WKH 435
+ + + Q++ ASP NGFF + GV+G +P AY+++ + F +V G++ ++L+E W+
Sbjct: 293 TRA-EYQILLASPEVNGFFGAKGVAGAIPAAYVHIERQFFSEVCSLGQQERVQLQEYWR- 350
Query: 436 GIVNQANGWSYHAKGLWLSEPGNDKRPCLTIVGSSNYTRRAYSLDIETNAIVLTKDEQLK 495
GW++HAKGLWL G+ PCLT++GS N+ R+ D+E ++T+++ L+
Sbjct: 351 ------RGWTFHAKGLWLYLAGS-SLPCLTLIGSPNFGYRSVHRDLEAQIAIVTENQALQ 403
Query: 496 TAMQNEIDNLLKNTKEVTLGSFEEDPDRHISTGVKLATKILGN 538
+ E + L + V+ +FE+ P R + VK+ T ++ N
Sbjct: 404 QQLHQEQEQLYLRSGVVSSATFEQ-PSRQVKLWVKMVTPLIKN 445
>CE25321 [I] KOG3964 Phosphatidylglycerolphosphate synthase
Length = 318
Score = 105 bits (261), Expect = 2e-22
Identities = 66/219 (30%), Positives = 119/219 (54%), Gaps = 18/219 (8%)
Query: 318 TIVYPVSQFTPLFQPHHDVSTEKPGVLSILSCLSNPMSRWTFTAGYFNMLPEIKQRLLDS 377
T +YPV Q L H + L L L NP + T +GYFN + + ++ +L
Sbjct: 114 TWIYPVLQMG-LLGIHQEFE-----FLQKLFSLKNPELKMTMASGYFNFIRDYEESILKE 167
Query: 378 PSIQGQVITASPYANGFFQSPGVSGHLPDAYLYLSQGFLKDVHRHGKENHIKLREWKHGI 437
++TASP+ANGFF+S G S ++P Y +S FL+ +G+ N +K+ E++
Sbjct: 168 GDYHLDILTASPFANGFFESNGFSKYIPPLYSNISDQFLRKREINGRLN-VKMFEYRR-- 224
Query: 438 VNQANGWSYHAKGLWLSEPGNDKRPCLTIVGSSNYTRRAYSLDIETNAIVLTKDEQLKTA 497
W++HAKGLW +E N +T++GSSNY R+ D+E +V+T++ L
Sbjct: 225 ----EEWTFHAKGLW-AEHNNQ---LMTLIGSSNYGYRSVHRDLEAQVMVVTRNPTLIDR 276
Query: 498 MQNEIDNLLKNTKEVTLGSFEEDPDRHISTGVKLATKIL 536
+++E + L + + + + + ++ P+ HI V++ ++++
Sbjct: 277 LKDEKNLLFEYSSILDMAALQQ-PEHHIPPLVRVISRLI 314
>HsM13259370 [I] KOG3964 Phosphatidylglycerolphosphate synthase
Length = 286
Score = 104 bits (259), Expect = 4e-22
Identities = 63/209 (30%), Positives = 112/209 (53%), Gaps = 17/209 (8%)
Query: 318 TIVYPVSQFTPLFQPHHDVSTEKPGVLSILSCLSNPMSRWTFTAGYFNMLPEIKQRLLDS 377
T +YP+ Q P ++ TE L + ++ T GYFN+ +L +
Sbjct: 30 TWIYPLIQMKPFEIQVDEIVTET------LLTEAERGAKVYLTTGYFNLTQAYMDLVLGT 83
Query: 378 PSIQGQVITASPYANGFFQSPGVSGHLPDAYLYLSQGFLKDVHRHGKENHIKLRE-WKHG 436
+ + Q++ ASP NGFF + GV+G +P AY+++ + F +V G++ ++L+E W+
Sbjct: 84 RA-EYQILLASPEVNGFFGAKGVAGAIPAAYVHIERQFFSEVCSLGQQERVQLQEYWR-- 140
Query: 437 IVNQANGWSYHAKGLWLSEPGNDKRPCLTIVGSSNYTRRAYSLDIETNAIVLTKDEQLKT 496
GW++HAKGLWL G+ PCLT++GS N+ R+ D+E ++T+++ L+
Sbjct: 141 -----RGWTFHAKGLWLYLAGS-SLPCLTLIGSPNFGYRSVHRDLEAQIAIVTENQALQQ 194
Query: 497 AMQNEIDNLLKNTKEVTLGSFEEDPDRHI 525
+ E + L + V+ +FE+ P R +
Sbjct: 195 QLHQEQEQLYLRSGVVSSATFEQ-PSRQV 222
>CE27264 [I] KOG3964 Phosphatidylglycerolphosphate synthase
Length = 162
Score = 82.4 bits (202), Expect = 2e-15
Identities = 52/135 (38%), Positives = 83/135 (60%), Gaps = 7/135 (5%)
Query: 85 EVEILDHPVQFYSTLKNKISAAQNRVFMASLYLGK--TENDLINCISDSLKKNKDLKVYF 142
++EILD P +FY L ++ + R+ + SLYLG+ E +LI I + +K+ +++V
Sbjct: 17 DLEILDTPTRFYEKLLELTASTERRLALCSLYLGEGDLEKNLIAAIRERSEKS-EIEVTI 75
Query: 143 MVDGLRGTRETPNKCSASLLAELEKEFGERVEIRLYRTPEFVGW-KTLIPKRFNEGLGLQ 201
++D LRGTR N S + L K E+ +I L+ TPE G K ++P+R +E +GLQ
Sbjct: 76 LLDFLRGTRT--NSSGESSVTVL-KPISEKSKIYLFHTPELSGLVKRVLPQRADEIIGLQ 132
Query: 202 HMKIYGVDEEVILSG 216
HMK+Y D+ V++SG
Sbjct: 133 HMKLYIFDDNVLISG 147
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.135 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 34,289,263
Number of Sequences: 60738
Number of extensions: 1553940
Number of successful extensions: 3646
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 3619
Number of HSP's gapped (non-prelim): 9
length of query: 540
length of database: 30,389,216
effective HSP length: 111
effective length of query: 429
effective length of database: 23,647,298
effective search space: 10144690842
effective search space used: 10144690842
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)