ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI2305 good J KOG4133 Translation, ribosomal structure and biogenesis tRNA splicing endonuclease
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI2305 784599 785489 297
(297 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YAR008w [J] KOG4133 tRNA splicing endonuclease 201 1e-51
SPBC19C7.07c [J] KOG4133 tRNA splicing endonuclease 137 2e-32
Hs20544678 [J] KOG4133 tRNA splicing endonuclease 94 2e-19
Hs13129062 [J] KOG4133 tRNA splicing endonuclease 75 9e-14
7294360 [J] KOG4133 tRNA splicing endonuclease 58 1e-08
>YAR008w [J] KOG4133 tRNA splicing endonuclease
Length = 275
Score = 201 bits (510), Expect = 1e-51
Identities = 113/274 (41%), Positives = 172/274 (62%), Gaps = 14/274 (5%)
Query: 37 PVLYDVDDIAKIREHGVAGVLIGTLGGAPQQNVFLGVPLRLMMEEALWLVEVGHAVFRHV 96
P+++D+D I +R+ G+ GVL GTL A QQNVFL VPLRLM+E+ LWL A + +
Sbjct: 3 PLVFDIDHIKLLRKWGICGVLSGTLPTAAQQNVFLSVPLRLMLEDVLWLHLNNLADVKLI 62
Query: 97 M--GQDLIKSLKGLDGEKLQKFKDDRANDVAQ-QLEWKRANFLRKMKTLRGASDVAVPSD 153
G ++++ + G KL K +DR N + Q ++K+ + K+K + +D +
Sbjct: 63 RQEGDEIMEGITLERGAKLSKIVNDRLNKSFEYQRKFKKDEHIAKLKKIGRINDKTTAEE 122
Query: 154 VI----NERDEQLMKQSIFIETKDTSAVIEPSLNDQTIDQRKICEQIL----RQNEKLRL 205
+ + ++QL++ S+FI+ +TS ++ +D R +L RQ K++
Sbjct: 123 LQRLDKSSNNDQLIESSLFIDIANTSMILRDIRSDSDSLSRDDISDLLFKQYRQAGKMQ- 181
Query: 206 NYKVYKKLREMGYFMSPGARFGGRYVAYPGDPLRYHSHLTVQDARS-KDEEIDLLNMING 264
Y +YK LR+ GY +SPG RFGG+++AYPGDPLR+HSHLT+QDA +E IDL++MI+G
Sbjct: 182 TYFLYKALRDQGYVLSPGGRFGGKFIAYPGDPLRFHSHLTIQDAIDYHNEPIDLISMISG 241
Query: 265 ARLGTSVKKTWVLPGI-NENGDAEFYSVEWAGFG 297
ARLGT+VKK WV+ G+ E + F+S+EWAGFG
Sbjct: 242 ARLGTTVKKLWVIGGVAEETKETHFFSIEWAGFG 275
>SPBC19C7.07c [J] KOG4133 tRNA splicing endonuclease
Length = 284
Score = 137 bits (345), Expect = 2e-32
Identities = 95/280 (33%), Positives = 153/280 (53%), Gaps = 31/280 (11%)
Query: 38 VLYDVDDIAKIRE-HGVAGVLIGTLGGAPQQNVFLGVPLRLMMEEALWLVEVG--HAVFR 94
+++DV + R+ + + G L+GTL PQQNVFLG+P+ L EEA +L+E G + V
Sbjct: 16 LVFDVQAVEVFRKNYHILGTLVGTLPQLPQQNVFLGLPMELSKEEAFYLIEKGISYIVDD 75
Query: 95 HVMGQDLIKSLKGLDGEKLQKFKDDRANDVAQQLEWKRANFLRKMKTLRGASDVAVPSDV 154
+ + L+++ D ++ K + A D Q + K+ +K++ ++ +P D
Sbjct: 76 TKVHKQLLENTTKDDVKQCLKKRQSLAYD--QMIAAKKKENEKKIEIMKKLGRT-LPLDP 132
Query: 155 INERDEQLMKQS-IFIETKDTSAVIEPSLNDQTIDQRKICEQILRQNEKLRLN-YKVYKK 212
+N + S I ++T T + S+ND + + E L +N Y ++K
Sbjct: 133 LNYDEHDSFDLSWIPVDTVTTRIAEKSSMNDDFHKEEDVFEN-------LDINRYLMFKS 185
Query: 213 LREMGYFMSPGARFGGRYVAYPGDPLRYHSHLTVQDARSKDEEIDLLNMINGARLGTSVK 272
L + G++++PG RFG ++VAYPGD LRYHSH V ++ D+EI +L +I G RLGT+VK
Sbjct: 186 LVDTGFYLNPGLRFGCQFVAYPGDALRYHSHYLV-NSYKWDQEIPVLFLIGGGRLGTAVK 244
Query: 273 KTWVLPG-----INENGD----------AEFYSVEWAGFG 297
KTW++ G IN NG+ +S+EWAGFG
Sbjct: 245 KTWLIGGSNDRNINMNGEKSKEELLLLPVRHFSIEWAGFG 284
>Hs20544678 [J] KOG4133 tRNA splicing endonuclease
Length = 310
Score = 94.0 bits (232), Expect = 2e-19
Identities = 74/297 (24%), Positives = 131/297 (43%), Gaps = 41/297 (13%)
Query: 38 VLYDVDDIAKIREH-GVAGVLIGTLGGAPQQNVFLGVPLRLMMEEALWLVEVGHAVFRHV 96
+++ + + +RE GV G +G L P+QN LG+PL LM EEA L E+G
Sbjct: 12 LVWGAEAVQALRERLGVGGRTVGALPRGPRQNSRLGLPLLLMPEEARLLAEIGAVTLVSA 71
Query: 97 MGQD------LIKSLKGLDGEKLQK-------FKDDRANDVAQQLEWKRANFLRKMKTLR 143
D + S K E Q+ ++ R ++ +++ +A +K++
Sbjct: 72 PRPDSRHHSLALTSFKRQQEESFQEQSALAAEARETRRQELLEKITEGQAAKKQKLEQAS 131
Query: 144 GASDVAVPSDVINERDEQLMKQSIFIETKD---TSAVIEPSLNDQTIDQRKICEQILRQN 200
GAS ++++ E ++ +S+ PS + + + Q+
Sbjct: 132 GASSSQEAGSSQAAKEDETSDGQASGEQEEAGPSSSQAGPSNGVAPLPRSALLVQLATAR 191
Query: 201 EKL-----------------------RLNYKVYKKLREMGYFMSPGARFGGRYVAYPGDP 237
+ L Y +Y+ L E G+F+S +FGG ++ YPGDP
Sbjct: 192 PRPVKARPLDWRVQSKDWPHAGRPAHELRYSIYRDLWERGFFLSAAGKFGGDFLVYPGDP 251
Query: 238 LRYHSHLTVQDARSKDEEIDLLNMINGARLGTSVKKTWVLPGINENGDAEFYSVEWA 294
LR+H+H Q + ++ I L +++ RLGTSV+KT +L +G + S++WA
Sbjct: 252 LRFHAHYIAQ-CWAPEDTIPLQDLVAAGRLGTSVRKTLLLCSPQPDGKVVYTSLQWA 307
>Hs13129062 [J] KOG4133 tRNA splicing endonuclease
Length = 390
Score = 75.5 bits (184), Expect = 9e-14
Identities = 69/285 (24%), Positives = 115/285 (40%), Gaps = 55/285 (19%)
Query: 38 VLYDVDDIAKIREH-GVAGVLIGTLGGAPQQNVFLGVPLRLMMEEALWLVEVGHAVFRHV 96
+++ + + +RE GV G +G L P+QN LG+PL LM EEA L E+G
Sbjct: 12 LVWGAEAVQALRERLGVGGRTVGALPRGPRQNSRLGLPLLLMPEEARLLAEIGAVTLVSA 71
Query: 97 MGQD------LIKSLKGLDGEKLQK-------FKDDRANDVAQQLEWKRANFLRKMKTLR 143
D + S K E Q+ ++ R +V +++ +A +K++
Sbjct: 72 PRPDSRHHSLALTSFKRQQEESFQEQSALAAEARETRRQEVLEKITEGQAAKKQKLEQAS 131
Query: 144 GASDVAVPSDVINERDEQLMKQSIFIETKD---TSAVIEPSLNDQTIDQRKICEQILRQN 200
GAS ++++ E ++ +S+ PS + + + Q+
Sbjct: 132 GASSSQEAGSSQAAKEDETSDGQASGEQEEAGPSSSQAGPSNGVAPLPRSALLVQLATAR 191
Query: 201 EKL-----------------------RLNYKVYKKLREMGYFMSPGARFGGRYVAYPGDP 237
+ L Y +Y+ L E G+F+S +FGG ++ YPGDP
Sbjct: 192 PRPVKARPLDWRVQSKDWPHAGRPAHELRYSIYRDLWERGFFLSAAGKFGGDFLVYPGDP 251
Query: 238 LRYHSHLTVQDARSKDEEIDLLNMINGARLGTSVKKTWVLPGINE 282
LR+H+H Q +D TS KTW+L G E
Sbjct: 252 LRFHAHYIAQCWAPED---------------TSHSKTWLLLGALE 281
>7294360 [J] KOG4133 tRNA splicing endonuclease
Length = 258
Score = 58.2 bits (139), Expect = 1e-08
Identities = 53/211 (25%), Positives = 93/211 (43%), Gaps = 29/211 (13%)
Query: 113 LQKFKDDRANDVAQQLEWKRANFLRKM-------KTLRGASDVAVPSDVINERDEQLMKQ 165
+ K KD +A A++LE +A+F ++ KT + ++ + ++L+KQ
Sbjct: 50 VDKSKDLKAKPTAEKLEECKADFESRLLGQEEALKTEKLRETERYMDKILTGKRKKLLKQ 109
Query: 166 S---IFIETKDTSAVIEPSLNDQTIDQRKI----CEQILRQNEKL---------RLNYKV 209
+ D ++E S+N + Q + CE + ++ L Y+V
Sbjct: 110 GKNELAAALTDKDVLLEMSVNFKFDRQNALLEVHCEHPTDHSAQIVTGPIVHTNSLKYRV 169
Query: 210 YKKLREMGYFMSPGARFGGRYVAYPGDPLRYH-SHLTVQDARSKDEEIDLLNMINGARLG 268
+K L G +++ G FG ++ YPGDP+ YH SH+ + + I L +I RL
Sbjct: 170 FKDLWRRGKYVTSGDAFGADFLVYPGDPMIYHASHIVI---LHEQPVIKPLELIAKVRLS 226
Query: 269 TSVKKTWVLPGINENGDAE--FYSVEWAGFG 297
V K+ V EN + E + +V W G
Sbjct: 227 VIVNKSCVFAYEMENNEEEIHYQTVAWCNLG 257
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.137 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,170,742
Number of Sequences: 60738
Number of extensions: 782476
Number of successful extensions: 1885
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1871
Number of HSP's gapped (non-prelim): 7
length of query: 297
length of database: 30,389,216
effective HSP length: 106
effective length of query: 191
effective length of database: 23,950,988
effective search space: 4574638708
effective search space used: 4574638708
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)