ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI2374 good Z KOG2001 Cytoskeleton Gamma-tubulin complex, DGRIP84/SPC97 component
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI2374 801373 803652 760
(760 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YHR172w [Z] KOG2001 Gamma-tubulin complex DGRIP84/SPC97 component 288 2e-77
SPBC365.15 [Z] KOG2001 Gamma-tubulin complex DGRIP84/SPC97 compo... 139 2e-32
7292918 [Z] KOG2000 Gamma-tubulin complex DGRIP91/SPC98 component 52 3e-06
>YHR172w [Z] KOG2001 Gamma-tubulin complex DGRIP84/SPC97 component
Length = 823
Score = 288 bits (737), Expect = 2e-77
Identities = 213/737 (28%), Positives = 360/737 (47%), Gaps = 77/737 (10%)
Query: 21 GRRVNPAPLCSEEIKVRTYPIEKTS---NDRVQESLVVRDLLLVLQGYEGVYIRYNSSYR 77
G+ VN PL S K++++ +EK S RVQE+LVV+DLL VL G EG YIRY + Y
Sbjct: 23 GKLVNHQPLWSTNPKLKSFSLEKISAPDQRRVQEALVVKDLLNVLIGLEGTYIRYFNDYE 82
Query: 78 -RDSMTGPEYKVVKMMNVTFKSFSKKLIKIGSIFVKLDKFQQWCCDERYGRVMHRLGHEV 136
D T E+K+ K M+ +FK+FS+++++ G ++ L + + D +G V+ R +E+
Sbjct: 83 PSDPETPIEFKIAKKMDPSFKTFSRRIVRYGKQYMILTRAYEKWSDTSFGMVLQRFAYEI 142
Query: 137 RIFLYENYLSCIKE-LEIRFQQDVKFSIRDLEMVLEDGFIYK-FRLLDQIVDRIMDSTRE 194
R FL + YL + E LE F + FSIR+LE ++ + + K LL I + I E
Sbjct: 143 RRFLEDVYLKTLVERLERDFNKVPNFSIRELEQIINETEVNKQMELLYNIYEEIFREIEE 202
Query: 195 RSEMDRVQMDFNNFMDDLRRDTNDELDLRVLYDTRVSLFVKGGSLXXXXXXXXXXXXGDI 254
R Q DFNNFMD ++ +++ L L V +DT V KGG++ GD
Sbjct: 203 RRTNQSSQEDFNNFMDSMKNESSLHLRLMVAFDTTVYPVPKGGAILKIFQQKILENLGDR 262
Query: 255 RHVEFLSLICDSLNRVYGQMFVEWMQEGKLNDYHEEFLISDTVADVDEEKLANTID-NER 313
V FL + +++++ Y M EW+ +G LND ++EF+ D + E K N D +R
Sbjct: 263 SSVMFLKKLLNNISQDYCTMLYEWLTQGILNDPYQEFMTYDDL----EGKTDNIFDTRDR 318
Query: 314 LWDTRFVIRKDGLL---PQFRDKELQQKILMTGKIWNLIAICCGNTALKPRSWGTSVTNI 370
WDT++ IRKD LL DK L K+L TG + ++ + S ++ I
Sbjct: 319 AWDTQYFIRKDVLLRDCDSEEDKNLLFKMLRTGILLKVVRASLQIPTIPSNSSDITIQEI 378
Query: 371 ----DITNMTFLKGYVNECYQQANEKCLKMYYEGYHMDKFLNLIHDQSYVHGVIDQLWNT 426
D+ + L+ YV++CY +ANE LK++++GY + L H Q G
Sbjct: 379 NDFADLMEGSNLELYVDKCYSRANEIFLKLFFQGYDLINVLK--HLQQIFLGYQSGHNVL 436
Query: 427 KFLSKTLFELT---RSPTESTLHKIQRHFDDILKEISVEDTYRHMILSKLTSVKIDTCPF 483
KFL+K + ELT R+ + K+ ++F E+ + + ++ +L ++ DT
Sbjct: 437 KFLTKNMGELTKHYRNDNNANYDKLLQNF-----ELERQSENPNNLMRQLLMIQFDTETL 491
Query: 484 YETLRQFI------------DEQETGLLNADNFQQLKGML---VAEEQNHTSQGSNELDE 528
+ L ++ ++ L++A+NF+ + +L +++E+ GSN
Sbjct: 492 PQVLSHYLQIYPEVPENNSANDDSDPLMHANNFKNMNAILFDELSKERTGAYHGSNLELY 551
Query: 529 GRKSKKKKNATPTAMYVQFETSIPYPLNIIWTKPVMVQYQMIQRLLLILSYHERLLEDTW 588
KS +++F+ +IPYPLNII ++ M++YQ+I R L+L YH RLL++TW
Sbjct: 552 TPKS--------AIYHLKFDINIPYPLNIIISRTCMIKYQIILRYQLVLQYHSRLLDETW 603
Query: 589 FEISKNKHWRRNSSQQSEYETHGMIFEXXXXXXXXGVRYFYVRGQCIRLARILHFQMRSM 648
+++K W+ + V+ + +R R+LH +M
Sbjct: 604 MDLNKTPSWKYRGYSHT------------------------VKRRIVRATRVLHAKMNHF 639
Query: 649 VKSKRNQVXXXXXXXXXXXXXXXHPMQEDLLQIQQHVQDHVTNIVSLSGLTSLPQIELQE 708
+K+ + + IQ ++ +TNI++ L+ L ++LQ
Sbjct: 640 IKTIMEYFNQNVIDKEVYSLEKCYRNPTLAVAIQNELEGGLTNIMTNRCLSDLIPLQLQ- 698
Query: 709 ATLHVIHSFIRFVASWR 725
+++ F +F+ S R
Sbjct: 699 -IFDIVYKFCKFIKSMR 714
>SPBC365.15 [Z] KOG2001 Gamma-tubulin complex DGRIP84/SPC97 component
Length = 784
Score = 139 bits (349), Expect = 2e-32
Identities = 152/642 (23%), Positives = 273/642 (41%), Gaps = 84/642 (13%)
Query: 3 DRGVVESVFLLNDKVGPFGRRVNPAPLCSEEIKVRTYPIEKTSNDRVQESLVVRDLLLVL 62
+ G V V L ++ V PF R+ L +T+ N QE+ ++ +LL
Sbjct: 24 EEGSVYHVSLKSEGVSPFSDRIQLNYLYDG----KTFSDPSNLNIHQQEACLINELLNAF 79
Query: 63 QGYEGVYIRYN----SSYRRDSMTGPEYKVVKMMNVTFKSFSKKLIKIGSIFVKLDKFQQ 118
G EGV++ SS + P + ++ ++ K + +++++GS ++ + F +
Sbjct: 80 MGMEGVFVHLQDMKASSEFETIIMPPSFYILPGFDLGIKDIASEMLEMGSHYLSITAFIE 139
Query: 119 WCCDERYGRVMHRLGHEVRIFLYENYLSCIKELEIRFQQDVKFSIRDLEMVLEDGFIYKF 178
YG V H L +R F+ + Y+ I + E + + D FS++ L + +
Sbjct: 140 SRSHFEYGFVNHALCAALRKFVMD-YVVLIMQCENQSRIDPNFSLQTLRLYT----LPTS 194
Query: 179 RLLDQIV----DRIMDSTRERSEMDRVQMDFNNFMDDLRRDTNDELDL-RVLYDTRVSLF 233
R L Q+ D ++ + S D + + + +DDL N+ D+ V+ TR
Sbjct: 195 RSLRQVYLILRDLLLSMEKNASSSDDLGL---SNIDDLLEQLNEGNDISHVVNATRSKKK 251
Query: 234 V-KGGSLXXXXXXXXXXXXGDIRHVEFLSLICDSLNRVYGQMFVEWMQEGKLNDYHEEFL 292
V KGG + GD + L+ + +R Y +M EW+ G +ND ++EF+
Sbjct: 252 VCKGGQVISFLTESLTKYAGDPVARKILTYLLREASRPYTKMLNEWIHLGLVNDPYDEFM 311
Query: 293 ISD----TVADVDEEKLANTIDNERLWDTRFVIRKDGLLPQFRDKELQQKILMTGKIWNL 348
I T +DE+ + W+ R+VIR+D + PQ D LQ K+L GK N+
Sbjct: 312 IKIHKGITSMQLDEDY------TDEYWEKRYVIREDQVPPQLLD--LQNKVLFAGKYLNV 363
Query: 349 IAIC---------------CGNTALKPRSWGTSVTNIDITNMTFLKGYVNECYQQANEKC 393
+ C N L P ++ ++I N Y NE Q +
Sbjct: 364 VLECRKGVNNLASLNAKDDTQNQLLWPSTFDDDNFTLNIMNAYV---YANESLLQLLQSS 420
Query: 394 LKMYYEGYHMDKFLNLIHDQSYVHGVIDQLWNTKFLSKTLFELTRSPTESTLHKIQRHFD 453
+Y Y + + L DQS T FL EL + ++ K+Q D
Sbjct: 421 QSLYAHLYSLKHYFFL--DQSDFF--------TTFLDNAQHELRKPAKYISITKLQSQLD 470
Query: 454 DILKE---ISVEDTYRHMILSKLTSVKIDTCPFYETLRQFIDE----------QETGLLN 500
L++ I+ D ++ + ++ + + ++E E+
Sbjct: 471 LALRQPGTITATDPHKEYVTVEVNQTSLIDWLMHIVSISGLEEGTSSQGNEVWNESITKQ 530
Query: 501 ADNFQQLKGMLVAEEQNHTSQGSNELDEGRKSKKKKNATPTAMYVQFETSIPYPLNIIWT 560
AD + + E N ++QG++++ S K N T +Q +P+PL++I +
Sbjct: 531 ADVGNETRNF--ESEHNRSTQGTSKVG----SDKDINGFET---MQLCYKVPFPLSLILS 581
Query: 561 KPVMVQYQMIQRLLLILSYHERLLEDTWFEISKNKHWRRNSS 602
+ +++YQ++ R L+L + E LE++W + SKN WR NSS
Sbjct: 582 RKAIIRYQLLFRYFLLLRHVEMQLENSWVQHSKNSAWRLNSS 623
>7292918 [Z] KOG2000 Gamma-tubulin complex DGRIP91/SPC98 component
Length = 917
Score = 52.4 bits (124), Expect = 3e-06
Identities = 49/161 (30%), Positives = 73/161 (44%), Gaps = 25/161 (15%)
Query: 258 EFLSLICDSLNRVYGQMFVEWMQEGKLNDYHEEFLISDTVADVDEEKLANTIDNERLWDT 317
+ L+ IC L R M +W+ EG ++D H EF + K + +RLW
Sbjct: 412 DLLTAICGPLVR----MISKWILEGGISDMHREFFV----------KSIKDVGVDRLWHD 457
Query: 318 RFVIRKDGLLPQFRDKELQQKILMTGKIWNLI-AICCGNTALKPRS-----WGTSVTNI- 370
+F +R +LP+F ++ KILMTGK N + IC +K R +S + I
Sbjct: 458 KFRLRLP-MLPKFVPMDMANKILMTGKSINFLREICEEQGMMKERDELMKVMESSASQIF 516
Query: 371 DITNMTFLKGYVNECYQQANEKCLKMYYEGYHMDKFLNLIH 411
T T V CYQQ ++ L + G H K L+ +H
Sbjct: 517 SYTPDTSWHAAVETCYQQTSKHVLDIMV-GPH--KLLDHLH 554
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.322 0.137 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,680,199
Number of Sequences: 60738
Number of extensions: 1761889
Number of successful extensions: 4477
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 4463
Number of HSP's gapped (non-prelim): 3
length of query: 760
length of database: 30,389,216
effective HSP length: 114
effective length of query: 646
effective length of database: 23,465,084
effective search space: 15158444264
effective search space used: 15158444264
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)