ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactI2427    suspect: LH   U     KOG0946  Intracellular trafficking, secretion, and vesicular transport  ER-Golgi vesicle-tethering protein p115

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactI2427 816911  818608 566  
         (566 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YDL058w [U] KOG0946 ER-Golgi vesicle-tethering protein p115            51   5e-06

>YDL058w [U] KOG0946 ER-Golgi vesicle-tethering protein p115
          Length = 1790

 Score = 50.8 bits (120), Expect = 5e-06
 Identities = 93/398 (23%), Positives = 167/398 (41%), Gaps = 81/398 (20%)

Query: 27   DSKKIYERLKSKVQFWKHQDVDGSSFN-TMDKRQQELWKLIRDSPLKFNQNNGAPESKEA 85
            +S+KI E L +K    K Q    ++ +   +K + EL +L + S     +   A E  E 
Sbjct: 1297 ESEKIKEELDAKTTELKIQLEKITNLSKAKEKSESELSRLKKTSS---EERKNAEEQLEK 1353

Query: 86   HASPLQP-----------IGESANTFLTDNDKDVN-VGDQFIKV---SPMKITALD--QS 128
              + +Q            + E ++T   +  + +N + D+ I++   + +K   +D  +S
Sbjct: 1354 LKNEIQIKNQAFEKERKLLNEGSSTITQEYSEKINTLEDELIRLQNENELKAKEIDNTRS 1413

Query: 129  PLFEVHSTTKELTGPSPPVLVERLTDKLHFKKVGLKRIQDRLGKIADSKENVARNYNKLA 188
             L +V  +  EL               L  K+  +K +QD   +I   K+ + RN  KL 
Sbjct: 1414 ELEKVSLSNDEL---------------LEEKQNTIKSLQD---EILSYKDKITRNDEKLL 1455

Query: 189  SEITAWCSLCLERDSELDLLTDLQQVLAADKKSEQTMTRLFMMINAKLEYVAKREENMLQ 248
            S         +ERD++ DL +  +Q+ AA +   +    L      KLE  + +E+  L+
Sbjct: 1456 S---------IERDNKRDLESLKEQLRAAQESKAKVEEGL-----KKLEEESSKEKAELE 1501

Query: 249  ERKDM----------NALTKKYDALRVRKGDQSMETQYLK-----ENLQRKRTSLQQL-- 291
            + K+M          N    K     +RK D+ +E          +NLQ +++ L     
Sbjct: 1502 KSKEMMKKLESTIESNETELKSSMETIRKSDEKLEQSKKSAEEDIKNLQHEKSDLISRIN 1561

Query: 292  -TEQYYESLSKILREEFTRACFTIYEMGSELKDVTRDFSLQSIELLKNGNDSDYIDGFLE 350
             +E+  E L   LR E         + GSEL+ V ++ +    ++  N  ++  +   LE
Sbjct: 1562 ESEKDIEELKSKLRIEA--------KSGSELETVKQELNNAQEKIRINAEENTVLKSKLE 1613

Query: 351  DVRKLRADKQWNKLSMQEKNN--PNKLAELVGNLYNGQ 386
            D+ +   DKQ    S QE+     ++L EL   L + Q
Sbjct: 1614 DIERELKDKQAEIKSNQEEKELLTSRLKELEQELDSTQ 1651


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.313    0.130    0.362 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,361,782
Number of Sequences: 60738
Number of extensions: 1380719
Number of successful extensions: 4053
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 4049
Number of HSP's gapped (non-prelim): 3
length of query: 566
length of database: 30,389,216
effective HSP length: 112
effective length of query: 454
effective length of database: 23,586,560
effective search space: 10708298240
effective search space used: 10708298240
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)