ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI2495 good R KOG3087 General function prediction only Serine/threonine protein kinase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI2495 843938 843150 -263
(263 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGR262c [R] KOG3087 Serine/threonine protein kinase 375 e-104
SPAP27G11.07c [R] KOG3087 Serine/threonine protein kinase 136 3e-32
Hs19923656 [R] KOG3087 Serine/threonine protein kinase 124 1e-28
At5g26110 [R] KOG3087 Serine/threonine protein kinase 118 1e-26
7295485 [R] KOG3087 Serine/threonine protein kinase 118 1e-26
CE26368_2 [R] KOG3087 Serine/threonine protein kinase 109 5e-24
ECU06g0400 [R] KOG3087 Serine/threonine protein kinase 79 7e-15
>YGR262c [R] KOG3087 Serine/threonine protein kinase
Length = 261
Score = 375 bits (963), Expect = e-104
Identities = 179/263 (68%), Positives = 211/263 (80%), Gaps = 2/263 (0%)
Query: 1 MSAEIIASIADVLTDNIPLTPISQGAEAVVFTSPVHPYLPKNRTDGDDKLYILKYRPEKK 60
M+ E I ++ LT ++ + PISQGAEA+VFT+ HPYLP+ + D K YI+KYRP K+
Sbjct: 1 MTQEFIDKVSSYLTPDVDIAPISQGAEAIVFTTTTHPYLPRAK-DSHQK-YIIKYRPPKR 58
Query: 61 YRHPVIDKTLTKHRTLGESRLLAKLRLIDGLNVPKLIGCDPYHGCIWLEFLGEDLPNGHG 120
YRHP ID+ LTKHRTL ESRLLAKL LI GL VP+LI CDPY+G IWLEFLGEDLP GHG
Sbjct: 59 YRHPQIDQALTKHRTLNESRLLAKLYLIPGLCVPQLIACDPYNGFIWLEFLGEDLPGGHG 118
Query: 121 FSNLKNFLWMNASDPYSDLVRDTMINVGKQIGLMHWNDYCHGDLTTSNIVLVRAGEDWQP 180
FSNLKNFLWM+ DPYSDLV T+ VG+QIGL+HWNDYCHGDLT+SNIVLVR G W P
Sbjct: 119 FSNLKNFLWMHDQDPYSDLVATTLRKVGRQIGLLHWNDYCHGDLTSSNIVLVRDGARWTP 178
Query: 181 YLIDFGLGSISTLVEDKGVDLYVLERAIISTHSSFANRYNLWVLEGFKSVFESHGKAGLG 240
+LIDFGLGS+S LVEDKGVDLYVLERAI+STHS A +YN W++EGF+ V+ G G
Sbjct: 179 HLIDFGLGSVSNLVEDKGVDLYVLERAILSTHSKHAEKYNAWIMEGFEEVYREQGAKGAK 238
Query: 241 KYKDLIRRFEEVRLRGRKRSMIG 263
K K++ +RFEEVRLRGRKRSM+G
Sbjct: 239 KLKEVTKRFEEVRLRGRKRSMLG 261
>SPAP27G11.07c [R] KOG3087 Serine/threonine protein kinase
Length = 238
Score = 136 bits (342), Expect = 3e-32
Identities = 89/245 (36%), Positives = 135/245 (54%), Gaps = 29/245 (11%)
Query: 19 LTPISQGAEAVVFTSPVHPYLPKNRTDGDDKLYILKYRPEKKYRHPVIDKTLTKHRTLGE 78
LT + QGAEA+ + +P ++ +LK RP K++RHP++D+ L++ R L E
Sbjct: 23 LTVVKQGAEAITIKTEFYP----------GEVCLLKCRPAKRWRHPILDQKLSRKRCLVE 72
Query: 79 SRLLAKLRLIDGLNVPKLIGCDPYHGCIWLEFLGEDLPNGHGFSNLKNFLWMNASDPYSD 138
+RLLAK + G+ P L D G I++E++ +++++
Sbjct: 73 ARLLAKCHYV-GIKCPMLYFIDANRGQIYMEWIDGPC--------VRDYIREICECEIEK 123
Query: 139 LVRDTMINVGKQIGLMHWNDYCHGDLTTSNIVLVRAGEDWQPYLIDFGLGSISTLVEDKG 198
+ M +G ++ MH ND HGDLTTSN++L + + P IDFGLGS+S EDK
Sbjct: 124 KLIPLMKRIGSEVAKMHKNDIVHGDLTTSNMML-ESHNNPVPIFIDFGLGSVSESEEDKA 182
Query: 199 VDLYVLERAIISTHSSFANRYNLWVLEGFKSVFESHGKAGLGKYKDLIRRFEEVRLRGRK 258
VD+YVLERA+ ST + F V +S+ ++ + K +RRFEEVR+RGRK
Sbjct: 183 VDIYVLERALSSTLPESESL--------FHHVLDSYAQS-WKQSKATLRRFEEVRMRGRK 233
Query: 259 RSMIG 263
R+MIG
Sbjct: 234 RTMIG 238
>Hs19923656 [R] KOG3087 Serine/threonine protein kinase
Length = 253
Score = 124 bits (312), Expect = 1e-28
Identities = 88/246 (35%), Positives = 130/246 (52%), Gaps = 35/246 (14%)
Query: 22 ISQGAEAVVFTSPVHPYLPKNRTDGDDKLYILKYRPEKKYRHPVIDKTLTKHRTLGESRL 81
+ QGAEA VF + R G + ++K+R K YRHP ++ L + RT+ E+R
Sbjct: 39 VKQGAEARVF---------RGRFQG--RAAVIKHRFPKGYRHPALEARLGRRRTVQEARA 87
Query: 82 LAKLRLIDGLNVPKLIGCDPYHGCIWLEFLGEDLPNGHGFSNLKNFLWMNASDPYSDLVR 141
L + R G++ P + D C+++E + G +++++ S ++
Sbjct: 88 LLRCRRA-GISAPVVFFVDYASNCLYMEEI-------EGSVTVRDYI---QSTMETEKTP 136
Query: 142 DTMINVGKQIGL----MHWNDYCHGDLTTSNIVLVRAGEDWQPYLIDFGLGSISTLVEDK 197
+ N+ K IG MH D HGDLTTSN++L E LIDFGL IS L EDK
Sbjct: 137 QGLSNLAKTIGQVLARMHDEDLIHGDLTTSNMLLKPPLEQLNIVLIDFGLSFISALPEDK 196
Query: 198 GVDLYVLERAIISTHSSFANRYNLWVLEGFKSVFESHGKAGLGKYKDLIRRFEEVRLRGR 257
GVDLYVLE+A +STH + V E F + + K K + ++++ +EVRLRGR
Sbjct: 197 GVDLYVLEKAFLSTHPNTET-----VFEAFLKSYSTSSK----KARPVLKKLDEVRLRGR 247
Query: 258 KRSMIG 263
KRSM+G
Sbjct: 248 KRSMVG 253
>At5g26110 [R] KOG3087 Serine/threonine protein kinase
Length = 295
Score = 118 bits (295), Expect = 1e-26
Identities = 82/212 (38%), Positives = 111/212 (51%), Gaps = 19/212 (8%)
Query: 52 ILKYRPEKKYRHPVIDKTLTKHRTLGESRLLAKLRLIDGLNVPKLIGCDPYHGCIWLEFL 111
I+K R KKYRHP++D LT R E+R + K R + G+ P L D + LE++
Sbjct: 103 IVKERFSKKYRHPILDAKLTLKRLNAEARCMTKARKL-GVCTPVLYAVDTLLHSLTLEYI 161
Query: 112 GEDLPNGHGFSNLKNFLWMNASDPYSDLVRDTMINVGKQIGLMHWNDYCHGDLTTSNIVL 171
G S FL + + + D +G I +H HGDLTTSN+ L
Sbjct: 162 -------EGVSVKDIFLEFGTNGVVEERLDDVAAQIGAAIAKLHDGGLAHGDLTTSNM-L 213
Query: 172 VRAGEDWQPYLIDFGLGSISTLVEDKGVDLYVLERAIISTHSSFANRYNLWVLEGFKSVF 231
VR+G + Q LIDFGL STL EDK VDLYVLERA++S HSS N V++ + +
Sbjct: 214 VRSGTN-QLVLIDFGLSVTSTLPEDKAVDLYVLERALLSMHSSCGN-----VMDRILTAY 267
Query: 232 ESHGKAGLGKYKDLIRRFEEVRLRGRKRSMIG 263
K ++ + +VR RGRKR+MIG
Sbjct: 268 RKSSK----QWSATFNKLAQVRQRGRKRTMIG 295
>7295485 [R] KOG3087 Serine/threonine protein kinase
Length = 224
Score = 118 bits (295), Expect = 1e-26
Identities = 77/216 (35%), Positives = 114/216 (52%), Gaps = 20/216 (9%)
Query: 52 ILKYRPEKKYRHPVIDKTLTKHRTLGESRLLAKLRLIDGLNVPKLIGCDPYHGCIWLEFL 111
++K R KKYRHP ++ +T+ R E++ + L G+ PK++ D +++E+
Sbjct: 25 LIKERFVKKYRHPELNTQITRQRMKAEAKASGRC-LAAGILAPKILHSDLNTHKLYMEYF 83
Query: 112 GEDLPNGHGFSNLKNFLWMNASDPYSDLVRDTMIN----VGKQIGLMHWNDYCHGDLTTS 167
K F+ S D + ++ +G+ IG MH N HGDLTTS
Sbjct: 84 D-------AAKTAKQFIQETVSTQTEDEAKKCLLEFCTRIGEIIGKMHSNHIIHGDLTTS 136
Query: 168 NIVLVRAGEDWQPYLIDFGLGSISTLVEDKGVDLYVLERAIISTHSSFANRYNLWVLEGF 227
NI++ G D+ LIDFGL + EDKGVDLYVLERA++STHS + VL +
Sbjct: 137 NILINPKGGDYDVILIDFGLSHYNQATEDKGVDLYVLERALLSTHSEQPYIFE-HVLAAY 195
Query: 228 KSVFESHGKAGLGKYKDLIRRFEEVRLRGRKRSMIG 263
++ +A ++ +FEEVR RGRKR+MIG
Sbjct: 196 RTACGKDEQA-------VLTKFEEVRARGRKRTMIG 224
>CE26368_2 [R] KOG3087 Serine/threonine protein kinase
Length = 215
Score = 109 bits (272), Expect = 5e-24
Identities = 83/215 (38%), Positives = 117/215 (53%), Gaps = 23/215 (10%)
Query: 52 ILKYRPEKKYRHPVIDKTLTKHRTLGESRLLAKLRLIDGLNVPKLIGCDPYHGCIWLEFL 111
I+K R K YRHP +D L K RT E R L K R + G++VP + D + +EF+
Sbjct: 21 IIKERFSKGYRHPTLDTQLNKARTKQEIRGLNKAREL-GIHVPAVYFIDNEKNQLIMEFV 79
Query: 112 GEDLPNGHGFSNLKNFLWMNASDP--YSDLVRDTMINVGKQIGLMHWNDYCHGDLTTSNI 169
S KN W++ +P + R+ G+++G +H HGDLTTSNI
Sbjct: 80 PG--------STAKN--WISQLNPADFDAKTREFGQIFGEKLGKLHRGGLIHGDLTTSNI 129
Query: 170 VLVRAGEDWQPYLIDFGLGSISTLV-EDKGVDLYVLERAIISTHSSFANRYNLWVLEGFK 228
+L R + + IDFGL S + E+KGVDLYVLERA+ISTH A ++EG
Sbjct: 130 IL-RDDDLQKMTFIDFGLSSQGKVTPEEKGVDLYVLERAVISTHDKCAA-----LIEG-- 181
Query: 229 SVFESHGKAGLGKYKDLIRRFEEVRLRGRKRSMIG 263
+ E + KA ++ + ++ E+RLRGRKR MIG
Sbjct: 182 -LMEGYKKADGKQFVAVEKKLNEIRLRGRKRDMIG 215
>ECU06g0400 [R] KOG3087 Serine/threonine protein kinase
Length = 197
Score = 79.0 bits (193), Expect = 7e-15
Identities = 74/236 (31%), Positives = 108/236 (45%), Gaps = 50/236 (21%)
Query: 24 QGAEAVVFTSPVHPYLPKNRTDGDDKLYILKYRPEKKYRHPVIDKTLTKHRTLGESRLLA 83
QGAE+++ DG+ ++K R K YR +D + RT E+++L
Sbjct: 6 QGAESII------------SADGET---VVKKRARKAYRIEALDLKIINGRTKREAKILR 50
Query: 84 KLRLIDGLNVPKLIGCDPYHGCIWLEFLGEDLPNGHGFSNLKNFLWMNASDPYSDLVRDT 143
KL + G+ P+LI Y I +E + + LK + ++SD L RD
Sbjct: 51 KLEAL-GIPAPRLIST--YGDTIVMEKVEGTV--------LKEMI--DSSDNPGALFRD- 96
Query: 144 MINVGKQIGLMHWNDYCHGDLTTSNIVLVRAGEDWQPYLIDFGLGSISTLVEDKGVDLYV 203
+G + +H D HGDLTTSN + + Y IDFGL IS EDK VDLYV
Sbjct: 97 ---LGALVSRLHIADIIHGDLTTSNFI-----HGSKIYAIDFGLSYISRKDEDKAVDLYV 148
Query: 204 LERAIISTHSSFANRYNLWVLEGFKSVFESHGKAGLGKYKDLIRRFEEVRLRGRKR 259
E+A+ H + LE F + G + ++++ VRLRGRKR
Sbjct: 149 FEKAVGCAHDA-------KFLEDFYCGYMGEGS------ESVLKKLGTVRLRGRKR 191
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.321 0.141 0.434
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,599,913
Number of Sequences: 60738
Number of extensions: 731568
Number of successful extensions: 1886
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1865
Number of HSP's gapped (non-prelim): 7
length of query: 263
length of database: 30,389,216
effective HSP length: 104
effective length of query: 159
effective length of database: 24,072,464
effective search space: 3827521776
effective search space used: 3827521776
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)