ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI2561 suspect: Pn U KOG0946 Intracellular trafficking, secretion, and vesicular transport ER-Golgi vesicle-tethering protein p115
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI2561 864111 865190 360
(360 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDL058w [U] KOG0946 ER-Golgi vesicle-tethering protein p115 54 3e-07
SPCC162.08c [S] KOG4674 Uncharacterized conserved coiled-coil pr... 54 4e-07
7303255 [Z] KOG0516 Dystonin GAS (Growth-arrest-specific protein... 51 3e-06
7298379 [ZR] KOG4568 Cytoskeleton-associated protein and related... 50 5e-06
>YDL058w [U] KOG0946 ER-Golgi vesicle-tethering protein p115
Length = 1790
Score = 54.3 bits (129), Expect = 3e-07
Identities = 63/325 (19%), Positives = 139/325 (42%), Gaps = 46/325 (14%)
Query: 45 EDIIEEEIPKVQENNERFE------HSVLSSEDTDFSGKLYALPTYNIRHINETXXXXXX 98
E+ +E+ ++Q N+ FE + S+ ++S K+ L IR NE
Sbjct: 1348 EEQLEKLKNEIQIKNQAFEKERKLLNEGSSTITQEYSEKINTLEDELIRLQNENELKA-- 1405
Query: 99 XXXXXXXXXXXDSEEDGSSNELQNLKQLYETLAQSTESTMENIQSQLVIASDEDTENEVT 158
E D + +EL+ + + L + ++T++++Q +++ D+ T N+
Sbjct: 1406 ------------KEIDNTRSELEKVSLSNDELLEEKQNTIKSLQDEILSYKDKITRNDEK 1453
Query: 159 LPNIRIEINQLKQLEDIEHKI-----SILESQIGPSNTNKSITSIINELHREINILKLDE 213
L + IE + + LE ++ ++ S + + G + + EL + ++K E
Sbjct: 1454 L--LSIERDNKRDLESLKEQLRAAQESKAKVEEGLKKLEEESSKEKAELEKSKEMMKKLE 1511
Query: 214 PTLTGFQNKMKEISDQYDQSIIGRRAQKDPNLSTDIERKLKSTESKVNDI-YDKYNILNK 272
T+ + ++K + +S +E+ KS E + ++ ++K +++++
Sbjct: 1512 STIESNETELKSSMETIRKS------------DEKLEQSKKSAEEDIKNLQHEKSDLISR 1559
Query: 273 YSEVIPQITERVQSLNVLHTSVNDMNDTVSEINDHMSSVHSQIEK---WSSLLESTSRQL 329
+E I E L + S +++ E+N+ + E+ S LE R+L
Sbjct: 1560 INESEKDIEELKSKLRIEAKSGSELETVKQELNNAQEKIRINAEENTVLKSKLEDIEREL 1619
Query: 330 TDREQSFKRTRHQLEL---QLNDLE 351
D++ K + + EL +L +LE
Sbjct: 1620 KDKQAEIKSNQEEKELLTSRLKELE 1644
>SPCC162.08c [S] KOG4674 Uncharacterized conserved coiled-coil protein
Length = 1837
Score = 53.9 bits (128), Expect = 4e-07
Identities = 64/270 (23%), Positives = 119/270 (43%), Gaps = 31/270 (11%)
Query: 114 DGSSNELQNLKQLYETLAQSTESTMENIQSQLVIASDEDT-------ENEVTL------P 160
DG+ + + L + ET+ Q T+ +E + QL + E ENE L P
Sbjct: 593 DGAEIQEETLIKANETIDQLTKM-LEEVSDQLRYSLKERDFFRSLVQENEKLLDMAPATP 651
Query: 161 NIRIEINQLKQ---------LEDIEHKISILESQIGPSNTNKSITSIINELHREINILKL 211
N ++ N ++Q LE + +++ L+S N K I+ L E + ++L
Sbjct: 652 NSKLNTNLIEQTSYQRSLIRLEQLTNELESLKSI--SRNKEKKFEEAISSLQLEKSNIQL 709
Query: 212 DEPTLTGFQNKMKEISDQYDQSIIGRRAQKDP--NLSTDIERKLKSTESKVNDIYDKYNI 269
+LT ++ E + ++S++ KD ++ +L S + +V ++ + +I
Sbjct: 710 QLTSLTSERSLALEKLNDLEKSLVLSERSKDELDESYKSLQEQLASKKIEVQNVSSQLSI 769
Query: 270 LNKYSEVIPQITERVQSLNVLHTSVND-MNDTVSEINDHMSSVHSQIEKWSSLLESTSRQ 328
N E I + ++S N+L TSV D + +S + +SS+ + +ES++++
Sbjct: 770 CNSQLEQSNHIVDNLKSENLLLTSVKDKLKADLSNLESKLSSLQQDNFHMKAQIESSNQE 829
Query: 329 LTDREQSFKRTRHQLELQLNDLEVRIGKLS 358
T S +L NDL V KLS
Sbjct: 830 YTATVDSMNSRILELS---NDLRVANSKLS 856
>7303255 [Z] KOG0516 Dystonin GAS (Growth-arrest-specific protein) and related
proteins
Length = 5385
Score = 50.8 bits (120), Expect = 3e-06
Identities = 48/214 (22%), Positives = 102/214 (47%), Gaps = 19/214 (8%)
Query: 119 ELQNLKQLYETLAQSTESTMENIQSQLVIASDEDTENEVTLPNIRIEINQLKQLEDIEHK 178
EL++L++ + T+A T +++ + + + + +T++ + +++ L LED EH
Sbjct: 1030 ELKHLQETFRTIALKTPVLKKSLDNLMELWKELNTQSGLHKDRLKLLEASLAGLEDNEHV 1089
Query: 179 ISILESQIGPSNTNKSITSIINELHREINILKLDEPTLTGFQNKMKEISDQYDQSIIGRR 238
IS LE+++ S + ++ +++N + + +T Q +M +++D DQ +GR
Sbjct: 1090 ISELENELARHQDLPSTAEGLQQVFKQLNHM---QDIITQQQPQMDKMNDAADQ--LGRM 1144
Query: 239 AQKDPNLSTDIERKLKSTESKVNDIYDKYNILNKYSEVIPQITERVQSLNVLHTSVNDMN 298
+ T + LK S V + ++S V Q+ ER++S T++ M
Sbjct: 1145 -----GVPTKVLGDLKRLHSNVE------RLNTRWSAVCNQLGERMRS---CETAIGLMK 1190
Query: 299 DTVSEINDHMSSVHSQIEKWSSLLESTSRQLTDR 332
+ S + S V E+ S++ +TS D+
Sbjct: 1191 NLQSSVQVEESWVDGTTERLSAMPTATSAYELDK 1224
>7298379 [ZR] KOG4568 Cytoskeleton-associated protein and related proteins
Length = 1690
Score = 50.1 bits (118), Expect = 5e-06
Identities = 62/267 (23%), Positives = 114/267 (42%), Gaps = 43/267 (16%)
Query: 112 EEDGSSNELQNLKQLYETLAQSTESTMENIQSQLVIASDEDTENEVTLPNIRIEINQLKQ 171
+E+ + + +NL+ L+ T++T++++Q +L I + E E +I LK
Sbjct: 1061 QEEVTKAKTENLE-----LSTGTQTTIKDLQERLEITNAELQHKEKMASEDAQKIADLKT 1115
Query: 172 LEDIEHKISILESQIGPSNTN-----------KSITSIINELHR---EINILKLDEPTLT 217
L + I + + I +N KS T+ I EL ++N +L E +T
Sbjct: 1116 LVEA---IQVANANISATNAELSTVLEVLQAEKSETNHIFELFEMEADMNSERLIEK-VT 1171
Query: 218 GFQNKMKEISDQYDQSIIGRRAQKDPNLSTDIERKLKSTESKVNDIYDKYNILNKYSEVI 277
G + ++KE Q D+ ++K + E K+ L + S+
Sbjct: 1172 GIKEELKETHLQLDER----------------QKKFEELEEKLKQAQQSEQKLQQESQTS 1215
Query: 278 PQ-ITERVQSLNVLHTSVNDMNDTVSEINDHMSSVHSQIEKWSSLLESTSRQLTDREQSF 336
+ +TE QSL L SV + V + + + S IE ++ L ++ QL ++
Sbjct: 1216 KEKLTEIQQSLQELQDSVKQKEELVQNLEEKVRESSSIIEAQNTKLNESNVQLENKTSCL 1275
Query: 337 KRTRHQL---ELQLNDLEVRIGKLSGQ 360
K T+ QL + + L+ KLSG+
Sbjct: 1276 KETQDQLLESQKKEKQLQEEAAKLSGE 1302
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.306 0.126 0.326
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,252,499
Number of Sequences: 60738
Number of extensions: 789802
Number of successful extensions: 3197
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 3177
Number of HSP's gapped (non-prelim): 18
length of query: 360
length of database: 30,389,216
effective HSP length: 108
effective length of query: 252
effective length of database: 23,829,512
effective search space: 6005037024
effective search space used: 6005037024
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (22.0 bits)