ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactI2678    suspect: LH   S     KOG4781  Function unknown                                               Uncharacterized conserved protein

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactI2678 900517  901281 255  
         (255 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YER182w [S] KOG4781 Uncharacterized conserved protein                 214   9e-56
SPAPB2B4.06 [S] KOG4781 Uncharacterized conserved protein              64   3e-10

>YER182w [S] KOG4781 Uncharacterized conserved protein
          Length = 244

 Score =  214 bits (545), Expect = 9e-56
 Identities = 110/232 (47%), Positives = 152/232 (65%), Gaps = 8/232 (3%)

Query: 25  SIAAAARQAKFVVKKPSSN--RKWTNVLIFGTCFGIGWFTTQHLTLTDIMAWWFYGKLPD 82
           +IA        VV K +S   R+W    IFG  F  GW+ TQH+T TD++A+W Y  LP 
Sbjct: 6   AIAQIRTYTNGVVFKTASKPKRRWIPWTIFGGSFLGGWYLTQHMTFTDLLAYWRYDALPK 65

Query: 83  TDSKLIDYRDELNQRCDNLSIVKQLLSKGYVAVYKQPDADATNTLIDKTLRAPGAICIPP 142
              +++ Y  +LN+R + L IVKQL + G+V V    +    N L+ + L  PG + IPP
Sbjct: 66  NADEVVKYHADLNRRLNGLPIVKQLENAGFVQVIANEEE---NLLVSRALNTPGGVAIPP 122

Query: 143 RIYYNPTTKDTVSVYHLGMKLTGYPFLVHGGILATVLEDTMRDSVKLVTGKS--AEVTQR 200
           R+YYNP+ ++TV +YHLGMKLTGYPFL+HGGILATV+ED M+++++L  G     + T+ 
Sbjct: 123 RVYYNPSRRETVGLYHLGMKLTGYPFLIHGGILATVIEDLMKEAIRLEKGTKNINQETKN 182

Query: 201 LKLNYSFPTLANQFVVVRRTKWEQKGKNDVEIEAELLDQSGRRPLVRGKANF 252
           L ++Y FPTLANQFVVVR T  +Q G N  +++AEL+DQSG R LV+  A F
Sbjct: 183 LSISYKFPTLANQFVVVRTTDLQQYG-NKTKLKAELMDQSGNRTLVKANATF 233


>SPAPB2B4.06 [S] KOG4781 Uncharacterized conserved protein
          Length = 245

 Score = 63.5 bits (153), Expect = 3e-10
 Identities = 39/147 (26%), Positives = 67/147 (45%), Gaps = 1/147 (0%)

Query: 93  ELNQRCDNLSIVKQLLSKGYVAVYKQPDADATNTLIDKTLRAPGAICIPPRIYYNPTTKD 152
           E+  R  NL IVK L         + P   + ++L +  L  PG I +PP I+Y+ +T+ 
Sbjct: 75  EVENRLQNLKIVKDLRQNPSFRELRMPFNRSNHSLTNNLLSGPGRITVPPVIFYDKSTRQ 134

Query: 153 TVSVYHLGMKLTGYPFLVHGGILATVLEDTMRDSVKLVTGKSAEVTQRLKLNYSFPTLAN 212
             ++ H+G  +      +H G++AT +++ +     L       VT  LKL+       N
Sbjct: 135 VYAIAHVGKDVGLDDDTIHPGLIATCMDEVLAICSFLSLPNKIAVTANLKLSNPTKAYTN 194

Query: 213 QFVVVR-RTKWEQKGKNDVEIEAELLD 238
            F ++R   +W +  K      A +LD
Sbjct: 195 HFYILRSHLEWTKGRKAQTHGTAYMLD 221


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.321    0.136    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,832,259
Number of Sequences: 60738
Number of extensions: 598396
Number of successful extensions: 1364
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1359
Number of HSP's gapped (non-prelim): 2
length of query: 255
length of database: 30,389,216
effective HSP length: 104
effective length of query: 151
effective length of database: 24,072,464
effective search space: 3634942064
effective search space used: 3634942064
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)