ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI2684 good H KOG3093 Coenzyme transport and metabolism 5-formyltetrahydrofolate cyclo-ligase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI2684 902216 901344 -291
(291 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YER183c [H] KOG3093 5-formyltetrahydrofolate cyclo-ligase 206 2e-53
Hs5453746 [H] KOG3093 5-formyltetrahydrofolate cyclo-ligase 102 6e-22
SPBC1703.08c [H] KOG3093 5-formyltetrahydrofolate cyclo-ligase 86 6e-17
CE20408 [H] KOG3093 5-formyltetrahydrofolate cyclo-ligase 83 4e-16
7291563 [H] KOG3093 5-formyltetrahydrofolate cyclo-ligase 82 7e-16
At5g13050 [H] KOG3093 5-formyltetrahydrofolate cyclo-ligase 63 4e-10
>YER183c [H] KOG3093 5-formyltetrahydrofolate cyclo-ligase
Length = 211
Score = 206 bits (525), Expect = 2e-53
Identities = 99/216 (45%), Positives = 149/216 (68%), Gaps = 7/216 (3%)
Query: 76 ISQKQVLRTQVKAMLRGLSQAELARQSEIVSRHALSLVNAKGFTHIACFLNMDHSEMKTE 135
++ KQ+LR Q+K ++ L +A +S +S+ SL+ + +AC+++MD E+ T
Sbjct: 1 MATKQLLRRQIKRVINALDYDIIAAESHTISQAVRSLIASANSRRVACYMSMDKGEVTTG 60
Query: 136 PLVESLFKQGKTVYLPRCSSTSVSKQVDLRQDGALKRPHLVFYRFDSLAQVQALQPQGKY 195
++++LF+ G+ V+LPRC+ TS SK LR+D PHL+F+R SL V+ L+PQG Y
Sbjct: 61 EIIKNLFQDGQEVFLPRCTHTSESKHFKLRED---HHPHLIFHRMSSLKMVRDLKPQGPY 117
Query: 196 KLREPVRTDPEPLPTPALEVILMPGVAFCKKNGARMGHGAGYYDDYISRH-IHYTGKKPL 254
+L+EP +P + L+V+L+PGVAF K GARMGHGAGYYDD+ R+ I + G+KPL
Sbjct: 118 QLKEP---EPHIEESDILDVVLVPGVAFDIKTGARMGHGAGYYDDFFQRYKILHEGQKPL 174
Query: 255 LVGLALEEQLVDDVPLEQHDYTVDCLVCGDGTVKWY 290
LVGL L EQ+ +PLE+HDY++DC+VCGDG++ W+
Sbjct: 175 LVGLCLMEQVASPIPLEKHDYSMDCIVCGDGSIHWF 210
>Hs5453746 [H] KOG3093 5-formyltetrahydrofolate cyclo-ligase
Length = 203
Score = 102 bits (254), Expect = 6e-22
Identities = 66/216 (30%), Positives = 115/216 (52%), Gaps = 20/216 (9%)
Query: 73 SAMISQKQVLRTQVKAMLRGLSQAELARQSEIVSRHALSLVNAKGFTHIACFLNMDHSEM 132
+A+ S K+ LR ++K LR +S E RQS ++S+ ++ + I+ FL+M E+
Sbjct: 4 AAVSSAKRSLRGELKQRLRAMSAEERLRQSRVLSQKVIAHSEYQKSKRISIFLSMQ-DEI 62
Query: 133 KTEPLVESLFKQGKTVYLPRCSSTSVSKQVDLRQDGALKRPHLVFYRFDSLAQVQALQPQ 192
+TE +++ +F++GK ++PR S H+ R +S ++ L P+
Sbjct: 63 ETEEIIKDIFQRGKICFIPRYRFQS---------------NHMDMVRIESPEEISLL-PK 106
Query: 193 GKYKLREPVRTD--PEPLPTPALEVILMPGVAFCKKNGARMGHGAGYYDDYISRHIHYTG 250
+ + +P D E L T L++I MPG+ F K+G R+G G GYYD Y+ R + +
Sbjct: 107 TSWNIPQPGEGDVREEALSTGGLDLIFMPGLGF-DKHGNRLGRGKGYYDAYLKRCLQHQE 165
Query: 251 KKPLLVGLALEEQLVDDVPLEQHDYTVDCLVCGDGT 286
KP + LA +EQ+ VP+ ++D VD ++ D +
Sbjct: 166 VKPYTLALAFKEQICLQVPVNENDMKVDEVLYEDSS 201
>SPBC1703.08c [H] KOG3093 5-formyltetrahydrofolate cyclo-ligase
Length = 204
Score = 85.9 bits (211), Expect = 6e-17
Identities = 61/223 (27%), Positives = 113/223 (50%), Gaps = 30/223 (13%)
Query: 75 MISQKQVLRTQVKAMLRGLSQAELARQSEIVSRHALSLVNAKGFTHIACFLNMDHSEMKT 134
M +K LR + + L L+ + QS + + + L K ++ ++NM E++T
Sbjct: 1 MSLKKNQLRAILNSSLGKLADHIIDSQSISICKQVVELPEWKRCKNVCLYMNMPKKEVRT 60
Query: 135 EPLVESLFKQGKTVYLPRCSSTSVSKQVDLRQDGALKRPHLVFYRFDSLA--QVQALQPQ 192
L++ FK+GK V++P+C + V + + VF + +SL + +P
Sbjct: 61 RCLIDVAFKEGKNVFIPKCIGSHVMEM------------YQVFEKTESLTINKWGIAEPN 108
Query: 193 GKYKLREPVRTDPEPLPTPALEVILMPGVAFCKKNGARMGHGAGYYDDYISRHIHYTGKK 252
G+ + TD E+I++PGVAF +K +R+GHG GYYD+YIS++ + +K
Sbjct: 109 GESRKIMDDETD--------CELIIVPGVAFDEKL-SRLGHGKGYYDNYISKYQSWALQK 159
Query: 253 P-----LLVGLALEEQLVD--DVPLEQHDYTVDCLVCGDGTVK 288
VG+ L+EQ++ ++P++ D +D LV + ++
Sbjct: 160 ESRANMFKVGICLKEQILPNREIPMDTRDQKLDALVTPEKVIR 202
>CE20408 [H] KOG3093 5-formyltetrahydrofolate cyclo-ligase
Length = 206
Score = 83.2 bits (204), Expect = 4e-16
Identities = 61/218 (27%), Positives = 105/218 (47%), Gaps = 21/218 (9%)
Query: 73 SAMISQKQVLRTQVKAMLRGLSQAELARQSEIVSRHALSLVNAKGFTHIACFLNMDHSEM 132
SA+ K LR +K +L +S+ E RQ+E V + + ++ +++ E+
Sbjct: 2 SAIKEVKSELRQFMKTLLGKISKEETQRQTEAVFEKIIESKWFQESKRLSVYVSTS-GEI 60
Query: 133 KTEPLVESLFKQGKTVYLPRCSSTSVSKQVDLRQDGALKRPHLVFYRFDSLAQVQ--ALQ 190
+T+ +++ + GK V++P+ + S + + D FD+L Q
Sbjct: 61 QTDSIIQKALEMGKEVFIPQFTKGSTAMDMVRVPDQTA---------FDNLPSTLWGIRQ 111
Query: 191 PQGKYKLREPVRTDPEPLPTPALEVILMPGVAFCKKNGARMGHGAGYYDDYIS-RHIHYT 249
P+ K+K + T P L++IL PGVAF G R GHG GYYD + S H H+
Sbjct: 112 PEPKWKWQSYHETGP-------LDLILAPGVAF-SPYGLRCGHGKGYYDRFFSTHHKHFP 163
Query: 250 GKKPLLVGLALEEQLVDDVPLEQHDYTVDCLVCGDGTV 287
P +GLAL EQ++ +P+ + D +D ++ T+
Sbjct: 164 ENSPKKIGLALREQIIGTIPISETDVELDEVIYEGETI 201
>7291563 [H] KOG3093 5-formyltetrahydrofolate cyclo-ligase
Length = 318
Score = 82.4 bits (202), Expect = 7e-16
Identities = 63/206 (30%), Positives = 106/206 (50%), Gaps = 20/206 (9%)
Query: 79 KQVLRTQVKAMLRGLSQAELARQSEIVSRHALSLVNAKGFTHIACFLNMDHSEMKTEPLV 138
K LR ++K L+G+ +ARQS+ V+ L + ++ +L+ SE+ T L+
Sbjct: 127 KVALRKRMKDALKGIDAEAIARQSQAVTAKVLQSEIFRQAQRVSIYLSTA-SELDTTALL 185
Query: 139 ESLFKQGKTVYLPRCSSTSVSKQVDLRQDGALKRPHLVFYRFDSLAQVQALQPQGKYKLR 198
+F+ K V++P + + K V LR + Y L + QP K + R
Sbjct: 186 SEMFRLEKMVFVPTYEGSRM-KMVRLRG--------MEEYESLPLTKWNIKQPDFK-EAR 235
Query: 199 EPVRTDPEPLPTPALEVILMPGVAFCKKNGARMGHGAGYYDDYISRHIH-YTGKKPLLVG 257
E T+ +++ ++PGVAF + GARMGHG GYYD ++ +H Y KK L+
Sbjct: 236 EDAMTNGH-----GIDLFIVPGVAFTRC-GARMGHGMGYYDKFLKQHAEKYPHKKISLMA 289
Query: 258 LALEEQLV--DDVPLEQHDYTVDCLV 281
L+L EQ+V +++P+E HD + ++
Sbjct: 290 LSLNEQIVSNEELPMESHDVRLHSVI 315
>At5g13050 [H] KOG3093 5-formyltetrahydrofolate cyclo-ligase
Length = 287
Score = 63.2 bits (152), Expect = 4e-10
Identities = 55/226 (24%), Positives = 107/226 (47%), Gaps = 25/226 (11%)
Query: 74 AMISQKQVLRTQVKAMLRGLSQAELARQSEIVSRHALS---LVNAKGF-THIAC--FLNM 127
++ QK+V+R+ V+ L+ + + +Q E + + L + KG +I+C +
Sbjct: 13 SIFKQKRVVRSTVRKSLKAMDPSLRTQQDEAIQKTVLEAPWFKSCKGLCAYISCKSLNEV 72
Query: 128 DHSEMKTEPLVESLFKQGKTVYLPRCSSTSVSKQVDLRQDGALKRPHLVFYRFDSLAQVQ 187
D S++ +E L K +Y+P + + ++ L H+ +S+ ++
Sbjct: 73 DTSKILSEILQHPDSNTQKKLYVPWVEDKNSNMRM-------LHISHMEDLVANSMNILE 125
Query: 188 ALQPQGKYKLREPVRTDPEPLPTPALEVILMPGVAFCKKNGARMGHGAGYYDDYISRHIH 247
+ RE V EP+ ++ ++PG+AF + G R+G G GYYD ++ R+
Sbjct: 126 PAPVDAQGNDREDVLQADEPI-----DLFILPGLAF-DRCGRRLGRGGGYYDTFLKRYQD 179
Query: 248 YTGKK----PLLVGLALEEQLVDD--VPLEQHDYTVDCLVCGDGTV 287
+K PL+V L+ Q+++D +P+ +D +D LV G V
Sbjct: 180 RAKEKGWRYPLMVALSYSPQILEDGSIPVTPNDVLIDALVTPSGVV 225
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.323 0.137 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,744,120
Number of Sequences: 60738
Number of extensions: 750441
Number of successful extensions: 1583
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 1569
Number of HSP's gapped (non-prelim): 6
length of query: 291
length of database: 30,389,216
effective HSP length: 105
effective length of query: 186
effective length of database: 24,011,726
effective search space: 4466181036
effective search space used: 4466181036
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)