ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI2705 good I KOG4178 Lipid transport and metabolism Soluble epoxide hydrolase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI2705 908981 908100 -294
(294 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNR064c [I] KOG4178 Soluble epoxide hydrolase 355 4e-98
At4g12830 [R] KOG1454 Predicted hydrolase/acyltransferase (alpha... 68 1e-11
At3g51000 [I] KOG4178 Soluble epoxide hydrolase 66 7e-11
Hs13376164 [I] KOG4178 Soluble epoxide hydrolase 65 2e-10
Hs22043561 [I] KOG4178 Soluble epoxide hydrolase 56 7e-08
Hs17449127 [I] KOG4178 Soluble epoxide hydrolase 56 7e-08
At3g05600 [I] KOG4178 Soluble epoxide hydrolase 56 7e-08
At4g36530 [R] KOG1454 Predicted hydrolase/acyltransferase (alpha... 55 9e-08
Hs4505155 [I] KOG4178 Soluble epoxide hydrolase 55 2e-07
7302539 [R] KOG2565 Predicted hydrolases or acyltransferases (al... 54 2e-07
At1g52510 [R] KOG1454 Predicted hydrolase/acyltransferase (alpha... 54 3e-07
CE28941 [I] KOG4178 Soluble epoxide hydrolase 53 6e-07
Hs4503583 [R] KOG2565 Predicted hydrolases or acyltransferases (... 52 1e-06
At5g19850_2 [R] KOG1454 Predicted hydrolase/acyltransferase (alp... 51 2e-06
7302538 [R] KOG2565 Predicted hydrolases or acyltransferases (al... 50 4e-06
>YNR064c [I] KOG4178 Soluble epoxide hydrolase
Length = 290
Score = 355 bits (911), Expect = 4e-98
Identities = 168/286 (58%), Positives = 213/286 (73%)
Query: 6 IQTFHTATLSNNTKVWYREAGDKSNPSIVLLHGYPTSSTMFRHLIPLISDKFHVIAPDLP 65
I FH + + KVWYREAG NP+I+LLHG+PTSS MFR+LIPL++ +FH+IAPDLP
Sbjct: 5 IARFHKIQVQDGVKVWYREAGAAGNPTILLLHGFPTSSNMFRNLIPLLAGQFHIIAPDLP 64
Query: 66 GFGFTEVPEDYHYSFDNLAATVREFLKEINVTRFAIYVFDYGSPVGFRLALKDPSSITGI 125
GFGFTE PE+Y +SFD+L ++ L +++ +FA+Y+FDYGSPVGFRLALK PS ITGI
Sbjct: 65 GFGFTETPENYKFSFDSLCESIGYLLDTLSIEKFAMYIFDYGSPVGFRLALKFPSRITGI 124
Query: 126 VTQNGNAYEEALGDEFWGFIKKYWQTTQTDPVFVKPLSQFIADRKNVDDQYFIGVKDKTA 185
VTQNGNAYEE L D FWG +K+YW++ Q+DPVFVK L ++ D NV QY GV +
Sbjct: 125 VTQNGNAYEEGLDDRFWGPLKEYWKSYQSDPVFVKSLIPYLEDPANVICQYHDGVPAIES 184
Query: 186 IEPAAYTLDFALLQRPGQTDIQLKLFHDYQNNLTIYPAIHEFLKAHRIPVLAAWGKNDYI 245
++PAAYTLD AL+QR GQTDIQL+LF DYQNN+ +YPA +FL+ +IPVL AWG ND I
Sbjct: 185 VDPAAYTLDIALIQRTGQTDIQLRLFFDYQNNIKLYPAFQKFLRDSKIPVLVAWGANDTI 244
Query: 246 FTPEGAEAFGKHAQKFKLTLLDSGHFALETHVQDIAHDINTFLHDN 291
F+ GAEA+ K K+ D+GHFALETHV IA +I + +N
Sbjct: 245 FSVAGAEAYRKDVDNLKVVYYDTGHFALETHVVAIAEEIISMFAEN 290
>At4g12830 [R] KOG1454 Predicted hydrolase/acyltransferase (alpha/beta
hydrolase superfamily)
Length = 319
Score = 68.2 bits (165), Expect = 1e-11
Identities = 31/113 (27%), Positives = 62/113 (54%), Gaps = 4/113 (3%)
Query: 21 WYR-EAGDKSNPSIVLLHGYPTSSTMFRHLIPLISDKFHVIAPDLPGFGFTEVPE---DY 76
W+ E+G +P ++L+HG+P+ + +R IP++S + IA D GFGF++ P+ +
Sbjct: 123 WFSVESGSVDSPPVILIHGFPSQAYSYRKTIPVLSKNYRAIAFDWLGFGFSDKPQAGYGF 182
Query: 77 HYSFDNLAATVREFLKEINVTRFAIYVFDYGSPVGFRLALKDPSSITGIVTQN 129
+Y+ D +++ F+ E+ ++ ++ V Y S + A P I ++ N
Sbjct: 183 NYTMDEFVSSLESFIDEVTTSKVSLVVQGYFSAAVVKYARNRPDKIKNLILLN 235
>At3g51000 [I] KOG4178 Soluble epoxide hydrolase
Length = 323
Score = 65.9 bits (159), Expect = 7e-11
Identities = 35/106 (33%), Positives = 59/106 (55%), Gaps = 2/106 (1%)
Query: 24 EAGDKSNPSIVLLHGYPTSSTMFRHLIPLISDK-FHVIAPDLPGFGFTE-VPEDYHYSFD 81
E GD+ P ++LLHG+P + +RH I +S +HV+APDL G+G ++ +P Y+
Sbjct: 21 EKGDEEGPLVLLLHGFPETWYSWRHQIDFLSSHGYHVVAPDLRGYGDSDSLPSHESYTVS 80
Query: 82 NLAATVREFLKEINVTRFAIYVFDYGSPVGFRLALKDPSSITGIVT 127
+L A V L T+ + D+G+ +G+ L L P + G ++
Sbjct: 81 HLVADVIGLLDHYGTTQAFVAGHDWGAIIGWCLCLFRPDRVKGFIS 126
>Hs13376164 [I] KOG4178 Soluble epoxide hydrolase
Length = 360
Score = 64.7 bits (156), Expect = 2e-10
Identities = 61/286 (21%), Positives = 123/286 (42%), Gaps = 20/286 (6%)
Query: 15 SNNTKVWYREAGDKSNPSIVLLHGYPTSSTMFRHLIPLISDKFHVIAPDLPGFGFTEVPE 74
S+ ++ Y AG + P ++ LHG+P + +R+ + +FHV+A DL G+G ++ P
Sbjct: 82 SSGLRLHYVSAGRGNGPLMLFLHGFPENWFSWRYQLREFQSRFHVVAVDLRGYGPSDAPR 141
Query: 75 DYH-YSFDNLAATVREFLKEINVTRFAIYVFDYGSPVGFRLALKDPSSITGIVTQNG--- 130
D Y+ D L +++ + + ++ + D+G+ + + ++ PS + +V +G
Sbjct: 142 DVDCYTIDLLLVDIKDVILGLGYSKCILVAHDWGALLAWHFSIYYPSLVERMVVVSGAPM 201
Query: 131 NAYEEALGDEFWGFIKKYWQTTQTDPVFVKPL---SQFIADRKNVDDQYFIGVKDKTAIE 187
+ Y++ F + ++ P + L S F + + + G+ T E
Sbjct: 202 SVYQDYSLHHISQFFRSHYMFLFQLPWLPEKLLSMSDFQILKTTLTHRK-TGIPCLTPSE 260
Query: 188 PAAYTLDFALLQRPGQTDIQLKLFHDYQNNLTIYPAIHEFLKAHRIPVLAAWGKNDYIFT 247
A+ +F+ +PG L + + N + P + P L WG+ D
Sbjct: 261 LEAFLYNFS---QPGGLTGPLNYYRNLFRNFPLEP------QELTTPTLLLWGEKDTYLE 311
Query: 248 PEGAEAFGKHAQKFKL---TLLDSGHFALETHVQDIAHDINTFLHD 290
EA G +L L GH+ +++ Q++ + FL D
Sbjct: 312 LGLVEAIGSRFVPGRLEAHILPGIGHWIPQSNPQEMHQYMWAFLQD 357
>Hs22043561 [I] KOG4178 Soluble epoxide hydrolase
Length = 237
Score = 55.8 bits (133), Expect = 7e-08
Identities = 27/109 (24%), Positives = 56/109 (50%), Gaps = 1/109 (0%)
Query: 22 YREAGDKSNPSIVLLHGYPTSSTMFRHLIPLISDKFHVIAPDLPGFGFTEVP-EDYHYSF 80
Y AG++ P ++LLHG+P +R+ + ++ V+A DL G+G T+ P +Y
Sbjct: 85 YVAAGERGKPLMLLLHGFPEFWYSWRYQLREFKSEYRVVALDLRGYGETDAPIHRQNYKL 144
Query: 81 DNLAATVREFLKEINVTRFAIYVFDYGSPVGFRLALKDPSSITGIVTQN 129
D L +++ L + ++ + D+G + + +A+ P + ++ N
Sbjct: 145 DCLITDIKDILDSLGYSKCVLIGHDWGGMIAWLIAICYPEMVMKLIVIN 193
>Hs17449127 [I] KOG4178 Soluble epoxide hydrolase
Length = 326
Score = 55.8 bits (133), Expect = 7e-08
Identities = 41/128 (32%), Positives = 67/128 (52%), Gaps = 11/128 (8%)
Query: 19 KVWYREA-GDKSNPSIV-LLHGYPTSSTMFRHLIPLISDKFH-VIAPDLPGFGFTEVPED 75
+++Y+++ G +P IV LLHG+PTSS + + ++ +FH VIA D GFGF++ P
Sbjct: 47 RIFYQDSVGVVGSPEIVVLLHGFPTSSYDWYKIWEGLTLRFHRVIALDFLGFGFSDKPRP 106
Query: 76 YHYSFDNLAATVREFLKEINV--TRFAIYVFDYGSPVGFRLALKDPSSITGIVT------ 127
+HYS A+ V L+ + + R + DYG V L + + +G +T
Sbjct: 107 HHYSIFEQASIVEALLRHLGLQNRRINLLSHDYGDIVAQELLYRYKQNRSGRLTIKSLCL 166
Query: 128 QNGNAYEE 135
NG + E
Sbjct: 167 SNGGIFPE 174
>At3g05600 [I] KOG4178 Soluble epoxide hydrolase
Length = 331
Score = 55.8 bits (133), Expect = 7e-08
Identities = 37/121 (30%), Positives = 58/121 (47%), Gaps = 4/121 (3%)
Query: 10 HTATLSNNTKVWYREAGDKSNPSIVLLHGYPTSSTMFRHLIP-LISDKFHVIAPDLPGFG 68
H N + E G K P ++LLHG+P +RH I L S + +APDL G+G
Sbjct: 6 HRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPDLRGYG 65
Query: 69 FTEVPEDY-HYSFDNLAATVREFLKEI--NVTRFAIYVFDYGSPVGFRLALKDPSSITGI 125
++ PE + Y+ N+ + L + N + + D+G+ +G+ L L P I G
Sbjct: 66 DSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPEKINGF 125
Query: 126 V 126
V
Sbjct: 126 V 126
>At4g36530 [R] KOG1454 Predicted hydrolase/acyltransferase (alpha/beta
hydrolase superfamily)
Length = 378
Score = 55.5 bits (132), Expect = 9e-08
Identities = 73/299 (24%), Positives = 113/299 (37%), Gaps = 45/299 (15%)
Query: 19 KVWYREAGDKSNPSIVLLHGYPTSSTMFRHLIPLISDKFHVIAPDLPGFGFTEVPEDYHY 78
K+ Y G+ S +VL+HG+ S +R+ IP ++ K+ V A DL GFG++ +
Sbjct: 90 KIHYVVQGEGS--PLVLIHGFGASVFHWRYNIPELAKKYKVYALDLLGFGWS---DKALI 144
Query: 79 SFDNLAAT--VREFLKEINVTRFAIYVFDYGSPVGFRLALKDPSSITGIVTQN------- 129
+D + T V +F+KE+ + G +A+ P +TG+ N
Sbjct: 145 EYDAMVWTDQVIDFMKEVVKEPAVVVGNSLGGFTALSVAVGLPEQVTGVALLNSAGQFAA 204
Query: 130 -GNAYEEALGDEFWGFIKK--------------YWQTTQTDPVFVKPLSQFIADRKNVDD 174
EEA FI K +WQ Q + L D NVDD
Sbjct: 205 ESRKREEADETVITKFIVKPLKEIFQRVVLGFLFWQAKQPSRI-ESVLKSVYIDSTNVDD 263
Query: 175 QYFIGVKDKTAIEPAAYTLDFALLQRPGQTDIQLKLFHDYQNNLTIYPAIHEFLKAHRIP 234
Y + K A +P A + + L+ R + N + Y + L P
Sbjct: 264 -YLVESISKPATDPNAGEVYYRLMTR-------------FLTNQSRY-TLDSVLSKMTCP 308
Query: 235 VLAAWGKNDYIFTPEGAEAFGKHAQKFKLTLLDSGHFALETHVQDIAHDINTFLHDNIA 293
+L WG D P AE L L +GH + + + + +L NIA
Sbjct: 309 LLLVWGDLDPWVGPAKAEKIKAFYSNSSLVHLQAGHCPHDEVPEAVNKALLDWLSINIA 367
>Hs4505155 [I] KOG4178 Soluble epoxide hydrolase
Length = 335
Score = 54.7 bits (130), Expect = 2e-07
Identities = 40/128 (31%), Positives = 66/128 (51%), Gaps = 11/128 (8%)
Query: 19 KVWYREA-GDKSNPSIV-LLHGYPTSSTMFRHLIPLISDKFH-VIAPDLPGFGFTEVPED 75
+++Y+++ G +P IV LLHG+PTSS + + ++ +FH VIA D GFGF++ P
Sbjct: 56 RIFYQDSVGVVGSPEIVVLLHGFPTSSYDWYKIWKGLTLRFHRVIALDFLGFGFSDKPRP 115
Query: 76 YHYSFDNLAATVREFLKEINV--TRFAIYVFDYGSPVGFRLALKDPSSITG------IVT 127
+HYS A+ V L+ + + R + DYG V L + + +G +
Sbjct: 116 HHYSIFEQASIVEALLRHLGLQNRRINLLSHDYGDIVAQELLYRYKQNRSGRHTIKSLCL 175
Query: 128 QNGNAYEE 135
NG + E
Sbjct: 176 SNGGIFPE 183
>7302539 [R] KOG2565 Predicted hydrolases or acyltransferases (alpha/beta
hydrolase superfamily)
Length = 463
Score = 54.3 bits (129), Expect = 2e-07
Identities = 30/99 (30%), Positives = 52/99 (52%), Gaps = 7/99 (7%)
Query: 33 IVLLHGYPTSSTMFRHLIPLI---SDK----FHVIAPDLPGFGFTEVPEDYHYSFDNLAA 85
++L+HG+P + F IPL+ SDK F VIAP LPG+G+++ + +A
Sbjct: 157 LLLMHGWPGTVREFYDFIPLLTTPSDKSDYVFEVIAPSLPGYGWSQGSSKTGFGVAQVAV 216
Query: 86 TVREFLKEINVTRFAIYVFDYGSPVGFRLALKDPSSITG 124
+R + + +F + D+GS +G +A P ++ G
Sbjct: 217 VMRNLMLRVGFDKFLVQGGDWGSIIGSNVASLFPENVLG 255
>At1g52510 [R] KOG1454 Predicted hydrolase/acyltransferase (alpha/beta
hydrolase superfamily)
Length = 330
Score = 53.9 bits (128), Expect = 3e-07
Identities = 41/141 (29%), Positives = 66/141 (46%), Gaps = 19/141 (13%)
Query: 2 TDSRIQTFHTATLSNNTKVWYREAGDKSNP--SIVLLHGYPTSSTMFRHLIPLISDK-FH 58
T+ + + + + S + + RE G K + +IV +HG PT S +R ++ +SD FH
Sbjct: 95 TEDKGKEYGSTIKSGKLRWFVRETGSKESRRGTIVFVHGAPTQSFSYRTVMSELSDAGFH 154
Query: 59 VIAPDLPGFGFTEVPE----------DYHYSFDNLAATVREFLKEINVTRFAIYVFDYGS 108
APD GFGF++ P+ +YH +FD L + E+ F + S
Sbjct: 155 CFAPDWIGFGFSDKPQPGYGFNYTEKEYHEAFDKLLEVL-----EVKSPFFLVVQVRMCS 209
Query: 109 PVGFRLALKDPSSITGIVTQN 129
G ALK+PS + + N
Sbjct: 210 -YGLTWALKNPSKVEKLAILN 229
>CE28941 [I] KOG4178 Soluble epoxide hydrolase
Length = 404
Score = 52.8 bits (125), Expect = 6e-07
Identities = 25/112 (22%), Positives = 55/112 (48%), Gaps = 1/112 (0%)
Query: 19 KVWYREAGDKSNPSIVLLHGYPTSSTMFRHLIPLISDKFHVIAPDLPGFGFTEVPEDY-H 77
++ Y + G P ++ +HGYP +R + +DK+ +A D G+ ++ P+ +
Sbjct: 128 RLHYVQTGSDDKPLMLFIHGYPEFWYSWRFQLKEFADKYRCVAIDQRGYNLSDKPKHVDN 187
Query: 78 YSFDNLAATVREFLKEINVTRFAIYVFDYGSPVGFRLALKDPSSITGIVTQN 129
YS D L +R+ ++ + + + D+G V ++ A + P + ++ N
Sbjct: 188 YSIDELTGDIRDVIEGLGYDKAIVVAHDWGGLVAWQFAEQYPEMVDKLICCN 239
>Hs4503583 [R] KOG2565 Predicted hydrolases or acyltransferases (alpha/beta
hydrolase superfamily)
Length = 455
Score = 51.6 bits (122), Expect = 1e-06
Identities = 42/140 (30%), Positives = 62/140 (44%), Gaps = 23/140 (16%)
Query: 25 AGDKSNPSIVLLHGYPTSSTMFRHLIPLISDK----------FHVIAPDLPGFGFTEVPE 74
AG P ++++HG+P S F +IPL++D F VI P +PG+GF+E
Sbjct: 137 AGHTPKP-LLMVHGWPGSFYEFYKIIPLLTDPKNHGLSDEHVFEVICPSIPGYGFSEASS 195
Query: 75 DYHYSFDNLAATVREFLK---EINVTRFAIYVFDYGSPVGFRLALKDPSSITG------I 125
+ N AT R F K + F I D+GS + +A PS + G +
Sbjct: 196 KKGF---NSVATARIFYKLMLRLGFQEFYIQGGDWGSLICTNMAQLVPSHVKGLHLNMAL 252
Query: 126 VTQNGNAYEEALGDEFWGFI 145
V N + LG F F+
Sbjct: 253 VLSNFSTLTLLLGQRFGRFL 272
>At5g19850_2 [R] KOG1454 Predicted hydrolase/acyltransferase (alpha/beta
hydrolase superfamily)
Length = 310
Score = 50.8 bits (120), Expect = 2e-06
Identities = 62/259 (23%), Positives = 105/259 (39%), Gaps = 17/259 (6%)
Query: 22 YREAGDKSNPSIVLLHGYPTSSTMFRHLIPLISDKFHVIAPDLPGFGFTEVPE------D 75
Y+ AG S P++VL+HG+ +S +R P++ V + DL G+G+++ P +
Sbjct: 38 YQCAGT-SGPALVLVHGFGANSDHWRKNTPILGKTHRVYSIDLIGYGYSDKPNPREFGGE 96
Query: 76 YHYSFDNLAATVREFLKEINVTRFAIYVFDYGSPVGFRLALKDPSSITGIVTQNGNAYEE 135
Y+F+ + +F ++ G VG + A+ P G++ N +
Sbjct: 97 PFYTFETWGEQLNDFCLDVVKDEAFFICNSIGGLVGLQAAVSKPEICRGLMLINISLRML 156
Query: 136 ALGDE-FWG--FIKKYWQTTQTDPVFVKPLSQFIADRKNVDDQYFIGVKDKTAIEPAAYT 192
+ + F G FIK + + PV K + IA + V D + +
Sbjct: 157 HIKKQPFIGRPFIKSFQNLLRNTPV-GKLFFKSIAKPETVKSILCQCYHDSSQVTDELVE 215
Query: 193 LDFALLQRPGQTDIQLKLFHDYQNNLTIYPAIHEFLKAHRIPVLAAWGKNDYIFTPEGAE 252
PG D+ L+ F Y P + L + PVL AWG+ D E
Sbjct: 216 AILRPGLEPGAVDVFLE-FICYSGG----PLPEDLLPLVKCPVLIAWGEKDPWEPIELGR 270
Query: 253 AFGK-HAQKFKLTLLDSGH 270
A+ A + + L D+GH
Sbjct: 271 AYSNFDAVEDFVVLPDAGH 289
>7302538 [R] KOG2565 Predicted hydrolases or acyltransferases (alpha/beta
hydrolase superfamily)
Length = 468
Score = 50.1 bits (118), Expect = 4e-06
Identities = 28/99 (28%), Positives = 50/99 (50%), Gaps = 7/99 (7%)
Query: 33 IVLLHGYPTSSTMFRHLIPLISDK-------FHVIAPDLPGFGFTEVPEDYHYSFDNLAA 85
++LLHG+P S F IP+++ F V+AP L G+G+++ ++ +A
Sbjct: 157 LLLLHGWPGSVREFFDFIPMLTKHSNITDYAFEVVAPSLVGYGWSDAATRPGFNAAEMAT 216
Query: 86 TVREFLKEINVTRFAIYVFDYGSPVGFRLALKDPSSITG 124
+R + + +F I D+GS +G LA P ++ G
Sbjct: 217 VMRNLMLRLGHKKFFIQGGDWGSIIGSNLATLYPENVIG 255
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.322 0.139 0.423
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,478,792
Number of Sequences: 60738
Number of extensions: 807538
Number of successful extensions: 1705
Number of sequences better than 1.0e-05: 15
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 1689
Number of HSP's gapped (non-prelim): 17
length of query: 294
length of database: 30,389,216
effective HSP length: 106
effective length of query: 188
effective length of database: 23,950,988
effective search space: 4502785744
effective search space used: 4502785744
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)