ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI2852 good L KOG2534 Replication, recombination and repair DNA polymerase IV (family X)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI2852 956798 955281 -506
(506 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YCR014c [L] KOG2534 DNA polymerase IV (family X) 191 2e-48
Hs7019491 [L] KOG2534 DNA polymerase IV (family X) 109 1e-23
Hs4505931 [L] KOG2534 DNA polymerase IV (family X) 86 2e-16
At1g10520 [L] KOG2534 DNA polymerase IV (family X) 71 4e-12
Hs17489401 [L] KOG2534 DNA polymerase IV (family X) 65 3e-10
Hs4758186 [L] KOG2534 DNA polymerase IV (family X) 64 4e-10
SPAC2F7.06c [L] KOG2534 DNA polymerase IV (family X) 55 2e-07
>YCR014c [L] KOG2534 DNA polymerase IV (family X)
Length = 582
Score = 191 bits (485), Expect = 2e-48
Identities = 145/579 (25%), Positives = 271/579 (46%), Gaps = 80/579 (13%)
Query: 2 LNGHKFLVLPNEDTSRRRYMRTLIDKNGG------QHGVE------IGLIDDSFVHGD-H 48
L G F LPN +TS ++ +++++K G Q+ ++ + LI+DSFV D H
Sbjct: 3 LKGKFFAFLPNPNTSSNKFFKSILEKKGATIVSSIQNCLQSSRKEVVILIEDSFVDSDMH 62
Query: 49 IINHKLFYKEMNPNDVP-----LDQ--LRLFKLSDLTKWLKHRFLDFSKP--LKLHEEVV 99
+ +F +E NDV ++Q ++ K S +TKW+++ F K +K ++
Sbjct: 63 LTQKDIFQREAGLNDVDEFLGKIEQSGIQCVKTSCITKWVQNDKFAFQKDDLIKFQPSII 122
Query: 100 VETDSQESNTGVPDTH-QVTSPSETHDTADTNVSHSSDAN-------------LIMPMNN 145
V +D+ + D ++++ E+ D+N A+ + +
Sbjct: 123 VISDNADDGQSSTDKESEISTDVESERNDDSNNKDMIQASKPLKRLLQGDKGRASLVTDK 182
Query: 146 PNYK----LIKVLKSLEKRYKLQNDTFRYLGYMKMIKILQNVRVKIESEEDAIEN--GLS 199
YK +I LK L K+Y+++ + FR Y + ++N + S E+A +
Sbjct: 183 TKYKNNELIIGALKRLTKKYEIEGEKFRARSYRLAKQSMENCDFNVRSGEEAHTKLRNIG 242
Query: 200 KGLSKKIPFLLKLGESGSELKMDTSEQTLLYFQECHGIGAFKSKQYVNMGYSTF------ 253
++KKI +L G E L YF+ C+GIG+ +K++ + + +F
Sbjct: 243 PSIAKKIQVILDTGVLPGLNDSVGLEDKLKYFKNCYGIGSEIAKRWNLLNFESFCVAAKK 302
Query: 254 --EDLIPYMNWTQLTGLAFYEDWQLSIPREETMKHEAIIRKAMNEVNENLCMEITGSYRR 311
E+ + +WT L G ++Y+DW + R E H ++KA+ ++ +E+ GSY R
Sbjct: 303 DPEEFVS--DWTILFGWSYYDDWLCKMSRNECFTHLKKVQKALRGIDPECQVELQGSYNR 360
Query: 312 GQPRSGDIDIVVHMPGQNDLNYISRELEKVIIKLTETGYIICPLNLNETLKSLFQPWFHK 371
G + GDID++ P ND +++ +E + IKL + GYI C L L L+ LF +
Sbjct: 361 GYSKCGDIDLLFFKPFCNDTTELAKIMETLCIKLYKDGYIHCFLQLTPNLEKLF---LKR 417
Query: 372 LFSHFNKPFDVKAY--------DEPVHKFYCGAAVTKYEPDRIK--------------DA 409
+ F + + Y E + KF+ G ++ E + +K +
Sbjct: 418 IVERF-RTAKIVGYGERKRWYSSEIIKKFFMGVKLSPRELEELKEMKNDEGTLLIEEEEE 476
Query: 410 DALTLKGNDKKYKMDI--GKTCRRVDFLLAEYQGLGATNVYFTGNNNFNKKCRTIAKQKG 467
+ LK D+ ++ G CRR+DF ++ LGA +++TG+ +N+ R +A QKG
Sbjct: 477 EETKLKPIDQYMSLNAKDGNYCRRLDFFCCKWDELGAGRIHYTGSKEYNRWIRILAAQKG 536
Query: 468 YVLSNDGIYKDGQLIPVADEYEILDMIGVDKLKPTERNL 506
+ L+ G++++ L+ +E I +++ + +P RN+
Sbjct: 537 FKLTQHGLFRNNILLESFNERRIFELLNLKYAEPEHRNI 575
>Hs7019491 [L] KOG2534 DNA polymerase IV (family X)
Length = 575
Score = 109 bits (272), Expect = 1e-23
Identities = 95/380 (25%), Positives = 160/380 (42%), Gaps = 75/380 (19%)
Query: 145 NPNYKLIKVLKSLEKRYKLQNDTFRYLGYMKMIKILQNVRVKIESEEDAIE-NGLSKGLS 203
N N + + L+ L K Y +Q D +R LGY K I L++ + S ++A G+ K ++
Sbjct: 251 NHNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQEACSIPGIGKRMA 310
Query: 204 KKIPFLLKLGESGSELKMD---TSEQTLLYFQECHGIGAFKSKQYVNMGYSTFEDLIPYM 260
+KI +++ ESG K+D S L F G G ++ + G+ + ED+
Sbjct: 311 EKI---IEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQMWYQQGFRSLEDIRSQA 367
Query: 261 NWT--QLTGLAFYEDWQLSIPREETMKHEAIIRKAMNEVNENLCMEITGSYRRGQPRSGD 318
+ T Q GL Y D+ +PREE + E ++KA N L GSYRRG+ GD
Sbjct: 368 SLTTQQAIGLKHYSDFLERMPREEATEIEQTVQKAAQAFNSGLLCVACGSYRRGKATCGD 427
Query: 319 IDIVV-HMPGQNDLNYISRELEKVIIKLTETGYIICPLNLNETLKSLFQPWFHKLFSHFN 377
+D+++ H G++ SR L+ L + G++ L
Sbjct: 428 VDVLITHPDGRSHRGIFSRLLD----SLRQEGFLTDDL---------------------- 461
Query: 378 KPFDVKAYDEPVHKFYCGAAVTKYEPDRIKDADALTLKGNDKKYKMDIGKTCRRVDFLLA 437
V + + Y G L G +++ RR+D ++
Sbjct: 462 ----VSQEENGQQQKYLGVC---------------RLPGPGRRH--------RRLDIIVV 494
Query: 438 EYQGLGATNVYFTGNNNFNKKCRTIAKQKGYVLSNDGI------------YKDGQLIPVA 485
Y +YFTG+ +FN+ R +AK KG LS + G+++P
Sbjct: 495 PYSEFACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGCKVGPGRVLPTP 554
Query: 486 DEYEILDMIGVDKLKPTERN 505
E ++ ++G+ +P ER+
Sbjct: 555 TEKDVFRLLGLPYREPAERD 574
>Hs4505931 [L] KOG2534 DNA polymerase IV (family X)
Length = 335
Score = 85.5 bits (210), Expect = 2e-16
Identities = 80/350 (22%), Positives = 148/350 (41%), Gaps = 63/350 (18%)
Query: 169 RYLGYMKMIKILQNVRVKIESEEDAIE-NGLSKGLSKKIPFLLKLGESGS--ELKMDTSE 225
+Y Y K ++ KI+S +A + G+ +++KI L G+ +++ D +
Sbjct: 35 KYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTS 94
Query: 226 QTLLYFQECHGIGAFKSKQYVNMGYSTFEDLIPY---MNWTQLTGLAFYEDWQLSIPREE 282
++ + GIG ++++V+ G T EDL +N Q GL ++ D++ IPREE
Sbjct: 95 SSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREE 154
Query: 283 TMKHEAIIRKAMNEVNENLCMEITGSYRRGQPRSGDIDIVVHMPG-QNDLNYISRELEKV 341
++ + I+ + +V+ + GS+RRG SGD+D+++ P ++ + L +V
Sbjct: 155 MLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQV 214
Query: 342 IIKLTETGYIICPLNLNETLKSLFQPWFHKLFSHFNKPFDVKAYDEPVHKFYCGAAVTKY 401
+ +L + +I L+ ET
Sbjct: 215 VEQLQKVHFITDTLSKGET----------------------------------------- 233
Query: 402 EPDRIKDADALTLKGNDKKYKMDIGKTCRRVDFLLAEYQGLGATNVYFTGNNNFNKKCRT 461
+ L K ++K+Y RR+D L +YFTG++ FNK R
Sbjct: 234 ---KFMGVCQLPSKNDEKEYPH------RRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRA 284
Query: 462 IAKQKGYVLSNDGIYK------DGQLIPVADEYEILDMIGVDKLKPTERN 505
A +KG+ ++ I G+ +PV E +I D I +P +R+
Sbjct: 285 HALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRS 334
>At1g10520 [L] KOG2534 DNA polymerase IV (family X)
Length = 491
Score = 70.9 bits (172), Expect = 4e-12
Identities = 64/255 (25%), Positives = 109/255 (42%), Gaps = 26/255 (10%)
Query: 103 DSQESNTGVPDTHQV-----TSPSETHDTADTNVSHSSDANLIMPMNNPNYKLIKVLKSL 157
+S + G PD+ TS S A+T S S++ + + N + ++ L
Sbjct: 152 ESHSNTQGSPDSPTSCSVPSTSASPGEGIAETPTSPQSESTSVYKPPDLNRNITEIFGKL 211
Query: 158 EKRYK---------------LQNDTFRYLGYMKMIKILQNVRVKIESEEDAIE-NGLSKG 201
Y+ + R Y K I +++ +IES + G+ K
Sbjct: 212 INIYRGTISGVCILDYSLIVTLGEDRRSFSYYKAIPVIEKFPTRIESVDQLKHLPGIGKA 271
Query: 202 LSKKIPFLLKLGESGSELKMDTSEQ--TLLYFQECHGIGAFKSKQYVNMGYSTFEDLIPY 259
+ I ++ G+ +T E+ T+ F E G+G + + G+ T EDL
Sbjct: 272 MRDHIQEIVTTGKLSKLEHFETDEKVRTISLFGEVWGVGPATALKLYEKGHRTLEDLKNE 331
Query: 260 MNWT--QLTGLAFYEDWQLSIPREETMKHEAIIRKAMNEVNENLCMEITGSYRRGQPRSG 317
+ T Q GL +++D + IPR+E + E ++++ E + + GSYRRG+ G
Sbjct: 332 DSLTHAQKLGLKYFDDIKTRIPRQEVQEMEQLLQRVGEETLPGVNIVCGGSYRRGKATCG 391
Query: 318 DIDIVV-HMPGQNDL 331
D+DIVV H GQ L
Sbjct: 392 DLDIVVTHPDGQRVL 406
>Hs17489401 [L] KOG2534 DNA polymerase IV (family X)
Length = 508
Score = 64.7 bits (156), Expect = 3e-10
Identities = 94/437 (21%), Positives = 172/437 (38%), Gaps = 65/437 (14%)
Query: 90 KPLKL---HEEVVVETDSQESNTGVPDTHQVTSPSETHDTADTNVSHSSDANLIMPMNNP 146
KP+++ H+ VV S +N G P T + + + +NN
Sbjct: 115 KPVEMTGKHQLVVRRDYSDSTNPGPPKTPPIAVQKISQYACQRRTT----------LNNC 164
Query: 147 NYKLIKVLKSLEKRYKLQNDTFRYLGYMKMIKILQNVRVKIESEEDAIENGLS------K 200
N L + + + + + +M+ +L+++ I S +D G+ K
Sbjct: 165 NQIFTDAFDILAENCEFRENEDSCVTFMRAASVLKSLPFTIISMKDT--EGIPCLGSKVK 222
Query: 201 GLSKKIPFLLKLGESGSELKM---DTSEQTLLYFQECHGIGAFKSKQYVNMGYSTFE--- 254
G+ ++I ++ GES SE+K D Q+ F G+G S+++ MG+ T
Sbjct: 223 GIIEEI---IEDGES-SEVKAVLNDERYQSFKLFTSVFGVGLKTSEKWFRMGFRTLSKVR 278
Query: 255 -DLIPYMNWTQLTGLAFYEDWQLSIPREETMKHEAIIRKAMNEVNENLCMEITGSYRRGQ 313
D Q G +YED + R E ++++A+ + + +TG +RRG+
Sbjct: 279 SDKSLKFTRMQKAGFLYYEDLVSCVTRAEAEAVSVLVKEAVWAFLPDAFVTMTGGFRRGK 338
Query: 314 PRSGDIDIVVHMPGQNDLNYISRELEKVIIKLTETGYIICPLNLNETLKSLFQPWFH-KL 372
D+D ++ PG + + L+KV+ + G ++ + T + L P
Sbjct: 339 KMGHDVDFLITSPGSTEDE--EQLLQKVMNLWEKKGLLLYYDLVESTFEKLRLPSRKVDA 396
Query: 373 FSHFNKPFDVKAYDEPVHKFYCGAAVTKYEPDRIKDADALTLKGNDKKYKMDIGKTCR-- 430
HF K F + K R+ D+D + GKT +
Sbjct: 397 LDHFQKCF----------------LIFKLPRQRV-DSD---------QSSWQEGKTWKAI 430
Query: 431 RVDFLLAEYQGLGATNVYFTGNNNFNKKCRTIAKQKGYVLSNDGIYKDGQ--LIPVADEY 488
RVD +L Y+ + +TG+ R ++ +L N +Y + + E
Sbjct: 431 RVDLVLCPYERRAFALLGWTGSRFERDLRRYATHERKMILDNHALYDKTKRIFLKAESEE 490
Query: 489 EILDMIGVDKLKPTERN 505
EI +G+D ++P ERN
Sbjct: 491 EIFAHLGLDYIEPWERN 507
>Hs4758186 [L] KOG2534 DNA polymerase IV (family X)
Length = 510
Score = 64.3 bits (155), Expect = 4e-10
Identities = 96/439 (21%), Positives = 175/439 (38%), Gaps = 67/439 (15%)
Query: 90 KPLKL---HEEVVVETDSQESNTGVPDTHQVTSPSETHDTADTNVSHSSDANLIMPMNNP 146
KP+++ H+ VV S +N G P T + + + +NN
Sbjct: 115 KPVEMTGKHQLVVRRDYSDSTNPGPPKTPPIAVQKISQYACQRRTT----------LNNC 164
Query: 147 NYKLIKVLKSLEKRYKLQNDTFRYLGYMKMIKILQNVRVKIESEEDAIENGLS------K 200
N L + + + + + +M+ +L+++ I S +D G+ K
Sbjct: 165 NQIFTDAFDILAENCEFRENEDSCVTFMRAASVLKSLPFTIISMKDT--EGIPCLGSKVK 222
Query: 201 GLSKKIPFLLKLGESGSELKM---DTSEQTLLYFQECHGIGAFKSKQYVNMGYSTFE--- 254
G+ ++I ++ GES SE+K D Q+ F G+G S+++ MG+ T
Sbjct: 223 GIIEEI---IEDGES-SEVKAVLNDERYQSFKLFTSVFGVGLKTSEKWFRMGFRTLSKVR 278
Query: 255 -DLIPYMNWTQLTGLAFYEDWQLSIPREETMKHEAIIRKAMNEVNENLCMEITGSYRRGQ 313
D Q G +YED + R E ++++A+ + + +TG +RRG+
Sbjct: 279 SDKSLKFTRMQKAGFLYYEDLVSCVTRAEAEAVSVLVKEAVWAFLPDAFVTMTGGFRRGK 338
Query: 314 PRSGDIDIVVHMPGQNDLNYISRELEKVIIKLTETGYIICPLNLNETLKSLFQPWFH-KL 372
D+D ++ PG + + L+KV+ + G ++ + T + L P
Sbjct: 339 KMGHDVDFLITSPGSTEDE--EQLLQKVMNLWEKKGLLLYYDLVESTFEKLRLPSRKVDA 396
Query: 373 FSHFNKPFDVKAYDEPVHKFYCGAAVTKYEPDRIKDADALTLKGNDKKYKMDIGKTCR-- 430
HF K F + K R+ D+D + GKT +
Sbjct: 397 LDHFQKCF----------------LIFKLPRQRV-DSD---------QSSWQEGKTWKAI 430
Query: 431 RVDFLLAEYQGLGATNVYFTGN-NNFNKKCRTIA-KQKGYVLSNDGIYKDGQ--LIPVAD 486
RVD +L Y+ + +TG+ F + R A ++ +L N +Y + +
Sbjct: 431 RVDLVLCPYERRAFALLGWTGSPRQFERDLRRYATHERKMILDNHALYDKTKRIFLKAES 490
Query: 487 EYEILDMIGVDKLKPTERN 505
E EI +G+D ++P ERN
Sbjct: 491 EEEIFAHLGLDYIEPWERN 509
>SPAC2F7.06c [L] KOG2534 DNA polymerase IV (family X)
Length = 506
Score = 55.1 bits (131), Expect = 2e-07
Identities = 48/192 (25%), Positives = 87/192 (45%), Gaps = 15/192 (7%)
Query: 142 PMNNPNYKLIKVLKSLEKRYKLQNDTFRYLGYMKMIKILQNVRVKIESEEDAIENGLSKG 201
P+ N + L L+ ++ ++ R Y I ++ + I+S E + G
Sbjct: 174 PLKCVNQAFVNALSVLKTCREVNGESVRTRAYGMAIATIKAFPLPIDSAEQLEK---MPG 230
Query: 202 LSKKIPFLLKLGESGSELK------MDTSEQTLLYFQECHGIGAFKSKQYVNMGYSTFED 255
KI L K S LK D + + LL F G+GA + ++ G+ T E
Sbjct: 231 CGPKIVHLWKEFASTGTLKEAEEFQKDPASKILLLFYNIFGVGASHAAEWYQKGWRTIEQ 290
Query: 256 LIPYMN-WTQL--TGLAFYEDWQLSIPREETMK-HEAIIRKAMNEVNENLCMEITGSYRR 311
+ + + +T+ GL FYED+ ++ EE + +E I+ + + + C+ G +RR
Sbjct: 291 VRKHKDSFTKQIKVGLEFYEDFCKTVTIEEATEIYETIVSRMPDGIKIQSCL--VGGFRR 348
Query: 312 GQPRSGDIDIVV 323
G+P D+D+V+
Sbjct: 349 GKPVGADVDMVL 360
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.137 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,324,116
Number of Sequences: 60738
Number of extensions: 1482087
Number of successful extensions: 3804
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 3784
Number of HSP's gapped (non-prelim): 11
length of query: 506
length of database: 30,389,216
effective HSP length: 111
effective length of query: 395
effective length of database: 23,647,298
effective search space: 9340682710
effective search space used: 9340682710
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)