ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactI2939    suspect: LH   S     KOG2585  Function unknown                                               Uncharacterized conserved protein

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactI2939 982022 980619 -468 
         (468 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YEL015w [S] KOG2585 Uncharacterized conserved protein                 173   6e-43

>YEL015w [S] KOG2585 Uncharacterized conserved protein
          Length = 551

 Score =  173 bits (438), Expect = 6e-43
 Identities = 158/554 (28%), Positives = 226/554 (40%), Gaps = 109/554 (19%)

Query: 1   MLNFKGYQIEIELKDGKRITGTLKQVSPKSLTLTDAVFQDGGVSPVFKIKADKLYDLKVL 60
           M  F G+ +++ELKDGK I G + + + K LTL D  F DGG S  FK++A +L DLKVL
Sbjct: 1   MSQFVGFGVQVELKDGKLIQGKIAKATSKGLTLNDVQFGDGGKSQAFKVRASRLKDLKVL 60

Query: 61  KLPPNANVXXXXXXXXXXXXXXXXXXXXXXXXRDRFTEKADEIWDTEMSSDFDFQGNLQR 120
            +   +                           +    + D++   +   DFDFQ NL  
Sbjct: 61  TVASQSGKRKQQRQQQQQNDYNQNRG-------EHIDWQDDDVSKIKQQEDFDFQRNLGM 113

Query: 121 FNKKDAFKDF-QNVEI---------------------PKDETKIP------------HNE 146
           FNKKD F    QN +I                       DE  IP             NE
Sbjct: 114 FNKKDVFAQLKQNDDILPENRLQGHNRKQTQLQQNNYQNDELVIPDAKKDSWNKISSRNE 173

Query: 147 MVTAGNVKETTSDSD---------------------KITESINITHLLR--------NDS 177
             T  +  +  +  D                      IT+S+NITHL+         ND 
Sbjct: 174 QSTHQSQPQQDAQDDLVLEDDEHEYDVDDIDDPKYLPITQSLNITHLIHSATNSPSINDK 233

Query: 178 IAASLANQR--------------------------KTFTCGSKN-KIKVPLATPIQLLEM 210
              ++ N +                          K  T  SKN K  +P+ATP+QLLEM
Sbjct: 234 TKGTVINDKDQVLAKLGQMIISQSRSNSTSLPAANKQTTIRSKNTKQNIPMATPVQLLEM 293

Query: 211 EKIADEKFMLPLKTSLEHSGIHISRLLRNMLKSYTPPATSDSDAVPTVVAFVS-GGRSGS 269
           E I  E F +     LE+  ++ S  L+  L         +S+  P VV   S   RSG+
Sbjct: 294 ESITSEFFSINSAGLLENFAVNASFFLKQKLGGRARLRLQNSNPEPLVVILASDSNRSGA 353

Query: 270 RCISALRCLLYIKDIRCIIVNPFINERESFDDDISTEQINKIKTLNN--VHFPDSLAKLK 327
           + ++  R L     IR I +  F   +    D +  +Q +  K      V+   SL    
Sbjct: 354 KALALGRHLCQTGHIRVITL--FTCSQNELQDSMVKKQTDIYKKCGGKIVNSVSSLESAM 411

Query: 328 ELLQNEPPSIIIDALQGFDDTVSDLDFSPDSNDN-IAQYIKWINSHETVSACLEVISFDC 386
           E L N P  I+IDA+QG+D T+SDL  + +  ++ I   I W N         +V S D 
Sbjct: 412 ETL-NSPVEIVIDAMQGYDCTLSDLAGTSEVIESRIKSMISWCNKQR---GSTKVWSLDI 467

Query: 387 PAHIDPSSG--DVIESDFVHPTTILSTGWPLHCLPKLTQILPDWRNTYVYDTGVPQQTYL 444
           P   D  SG  D+  SD +  T I+ +GWPL  +  L   LP   +  + D G+PQ  Y 
Sbjct: 468 PNGFDAGSGMPDIFFSDRIEATGIICSGWPLIAINNLIANLPSLEDAVLIDIGIPQGAYS 527

Query: 445 LKPNLRKFYKLDIY 458
            + +LRKF   D++
Sbjct: 528 QRTSLRKFQNCDLF 541


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.317    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,753,535
Number of Sequences: 60738
Number of extensions: 1189920
Number of successful extensions: 3102
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3096
Number of HSP's gapped (non-prelim): 4
length of query: 468
length of database: 30,389,216
effective HSP length: 110
effective length of query: 358
effective length of database: 23,708,036
effective search space: 8487476888
effective search space used: 8487476888
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)