ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI3049 good J KOG2916 Translation, ribosomal structure and biogenesis Translation initiation factor 2, alpha subunit (eIF-2alpha)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI3049 1021526 1022431 302
(302 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJR007w [J] KOG2916 Translation initiation factor 2 alpha subuni... 451 e-127
SPAC3G9.09c [J] KOG2916 Translation initiation factor 2 alpha su... 352 4e-97
Hs4758256 [J] KOG2916 Translation initiation factor 2 alpha subu... 293 2e-79
7293233 [J] KOG2916 Translation initiation factor 2 alpha subuni... 273 2e-73
At5g05470 [J] KOG2916 Translation initiation factor 2 alpha subu... 251 9e-67
CE29373 [J] KOG2916 Translation initiation factor 2 alpha subuni... 242 4e-64
ECU11g0930i [J] KOG2916 Translation initiation factor 2 alpha su... 116 5e-26
At2g40290 [J] KOG2916 Translation initiation factor 2 alpha subu... 114 1e-25
>YJR007w [J] KOG2916 Translation initiation factor 2 alpha subunit
(eIF-2alpha)
Length = 304
Score = 451 bits (1159), Expect = e-127
Identities = 220/291 (75%), Positives = 255/291 (87%)
Query: 1 MSTSNCRFYENKFPEVDDVVMVNVQQIAEMGAYVRLLEYDNIEGMVXXXXXXXXXXXXXQ 60
MSTS+CRFYENK+PE+DD+VMVNVQQIAEMGAYV+LLEYDNIEGM+ Q
Sbjct: 1 MSTSHCRFYENKYPEIDDIVMVNVQQIAEMGAYVKLLEYDNIEGMILLSELSRRRIRSIQ 60
Query: 61 KLIRVGKNEVVVVLRVDKEKGYIDLSKRRVSAEDVIKCEERYQKSKAVHSILRQCAEKYQ 120
KLIRVGKN+V VVLRVDKEKGYIDLSKRRVS+ED+IKCEE+YQKSK VHSILR CAEK+Q
Sbjct: 61 KLIRVGKNDVAVVLRVDKEKGYIDLSKRRVSSEDIIKCEEKYQKSKTVHSILRYCAEKFQ 120
Query: 121 VSLEDLYKAYAWPLSRKYGHAYDAYKMSIVDPNVWDGIESISDEIFQELKGQITRRLTPQ 180
+ LE+LYK AWPLSRK+GHAY+A+K+SI+D VW+GIE S ++ ELK I++RLTPQ
Sbjct: 121 IPLEELYKTIAWPLSRKFGHAYEAFKLSIIDETVWEGIEPPSKDVLDELKNYISKRLTPQ 180
Query: 181 AVKIRADVEVSCFSYEGIDAIKEALKSAEELSTEQMQIKVKLVAAPLYVITTQSLDKDQG 240
AVKIRADVEVSCFSYEGIDAIK+ALKSAE++STEQMQ+KVKLVAAPLYV+TTQ+LDK +G
Sbjct: 181 AVKIRADVEVSCFSYEGIDAIKDALKSAEDMSTEQMQVKVKLVAAPLYVLTTQALDKQKG 240
Query: 241 VQLLETAIAKIEEVILKYNGVCNVTMAPKAVTATEDAELQALLESKELEGR 291
++ LE+AI KI EVI KY GVCN+TM PKAVTATEDAELQALLESKEL+ R
Sbjct: 241 IEQLESAIEKITEVITKYGGVCNITMPPKAVTATEDAELQALLESKELDNR 291
>SPAC3G9.09c [J] KOG2916 Translation initiation factor 2 alpha subunit
(eIF-2alpha)
Length = 306
Score = 352 bits (903), Expect = 4e-97
Identities = 179/290 (61%), Positives = 222/290 (75%), Gaps = 1/290 (0%)
Query: 1 MSTSNCRFYENKFPEVDDVVMVNVQQIAEMGAYVRLLEYDNIEGMVXXXXXXXXXXXXXQ 60
MST++CR YEN+FPEVD++V+VNV+QI EMGAYV+LLEYDNIEGMV Q
Sbjct: 1 MSTTSCRMYENRFPEVDELVVVNVRQIQEMGAYVKLLEYDNIEGMVLLSELSRRRIRSVQ 60
Query: 61 KLIRVGKNEVVVVLRVDKEKGYIDLSKRRVSAEDVIKCEERYQKSKAVHSILRQCAEKYQ 120
K IRVG+NEVVVVLRVDKEKGYIDLSKRRVS EDV+KCEER+ KSKAVHSI+R AEK+
Sbjct: 61 KHIRVGRNEVVVVLRVDKEKGYIDLSKRRVSPEDVVKCEERFNKSKAVHSIMRHIAEKHN 120
Query: 121 VSLEDLYKAYAWPLSRKYGHAYDAYKMSIVDP-NVWDGIESISDEIFQELKGQITRRLTP 179
V LE +Y WPL RKYGHAYDA+K++I +P +V++G+E + +L QI+RRLTP
Sbjct: 121 VPLETMYTTIGWPLYRKYGHAYDAFKLAISNPDHVFEGLEPPKSGVINDLLAQISRRLTP 180
Query: 180 QAVKIRADVEVSCFSYEGIDAIKEALKSAEELSTEQMQIKVKLVAAPLYVITTQSLDKDQ 239
Q +KIRADVEV+CF YEGI+AIK ALK+AE++ TE++ IKVKLVA PLYV+ T +LDK
Sbjct: 181 QPIKIRADVEVTCFGYEGINAIKAALKAAEDVHTEEVPIKVKLVAPPLYVLLTNALDKSL 240
Query: 240 GVQLLETAIAKIEEVILKYNGVCNVTMAPKAVTATEDAELQALLESKELE 289
G++ LE AI IE+ I NG C V M PKAV+ T++ EL L++ E E
Sbjct: 241 GLKKLEEAIGAIEKSITASNGTCTVKMKPKAVSETDELELADLMKKFEKE 290
>Hs4758256 [J] KOG2916 Translation initiation factor 2 alpha subunit
(eIF-2alpha)
Length = 315
Score = 293 bits (751), Expect = 2e-79
Identities = 156/293 (53%), Positives = 204/293 (69%), Gaps = 9/293 (3%)
Query: 5 NCRFYENKFPEVDDVVMVNVQQIAEMGAYVRLLEYDNIEGMVXXXXXXXXXXXXXQKLIR 64
+CRFY++KFPEV+DVVMVNV+ IAEMGAYV LLEY+NIEGM+ KLIR
Sbjct: 5 SCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINKLIR 64
Query: 65 VGKNEVVVVLRVDKEKGYIDLSKRRVSAEDVIKCEERYQKSKAVHSILRQCAEKYQVS-- 122
+G+NE VVV+RVDKEKGYIDLSKRRVS E+ IKCE+++ KSK V+SILR AE + +
Sbjct: 65 IGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEYTKD 124
Query: 123 --LEDLYKAYAWPLSRKYGH----AYDAYKMSIVDPNVWDGIESISDEIFQELKGQITRR 176
LE L++ AW KY AYDA+K ++ DP++ D ++ DE + L I RR
Sbjct: 125 EQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDE-REVLINNINRR 183
Query: 177 LTPQAVKIRADVEVSCFSYEGIDAIKEALKSAEELSTEQMQIKVKLVAAPLYVITTQSLD 236
LTPQAVKIRAD+EV+C+ YEGIDA+KEAL++ STE M IK+ L+A P YV+TT +L+
Sbjct: 184 LTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTTTLE 243
Query: 237 KDQGVQLLETAIAKIEEVILKYNGVCNVTMAPKAVTATEDAELQALLESKELE 289
+ +G+ +L A+A I+E I + GV NV M PK VT T++ EL +E E E
Sbjct: 244 RTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQMERLERE 296
>7293233 [J] KOG2916 Translation initiation factor 2 alpha subunit
(eIF-2alpha)
Length = 341
Score = 273 bits (699), Expect = 2e-73
Identities = 148/290 (51%), Positives = 190/290 (65%), Gaps = 8/290 (2%)
Query: 7 RFYENKFPEVDDVVMVNVQQIAEMGAYVRLLEYDNIEGMVXXXXXXXXXXXXXQKLIRVG 66
RFY ++PE++DVVMVNV IAEMGAYV LLEY+NIEGM+ KLIRVG
Sbjct: 6 RFYNERYPEIEDVVMVNVLSIAEMGAYVHLLEYNNIEGMILLSELSRRRIRSINKLIRVG 65
Query: 67 KNEVVVVLRVDKEKGYIDLSKRRVSAEDVIKCEERYQKSKAVHSILRQCAE----KYQVS 122
K E VVV+RVDKEKGYIDLSKRRVS EDV KC ER+ K+KA++S+LR A+ +
Sbjct: 66 KTEPVVVIRVDKEKGYIDLSKRRVSPEDVEKCTERFAKAKAINSLLRHVADILGFEGNEK 125
Query: 123 LEDLYKAYAWPLSRKYGH---AYDAYKMSIVDPNVWDGIESISDEIFQELKGQITRRLTP 179
LEDLY+ AW +KY + AYD +K S+ DP V+D ++ E + L I R+L
Sbjct: 126 LEDLYQKTAWHFEKKYNNKTVAYDIFKQSVTDPTVFDEC-NLEPETKEVLLSNIKRKLVS 184
Query: 180 QAVKIRADVEVSCFSYEGIDAIKEALKSAEELSTEQMQIKVKLVAAPLYVITTQSLDKDQ 239
VKIRAD+E SC+ YEGIDA+K +L ELSTE++ I++ L+A PLYV+TT + K
Sbjct: 185 PTVKIRADIECSCYGYEGIDAVKASLTKGLELSTEELPIRINLIAPPLYVMTTSTTKKTD 244
Query: 240 GVQLLETAIAKIEEVILKYNGVCNVTMAPKAVTATEDAELQALLESKELE 289
G++ LE AI I +Y+G V MAPK VTA ++A+L LE E E
Sbjct: 245 GLKALEVAIEHIRAKTSEYDGEFKVIMAPKLVTAIDEADLARRLERAEAE 294
>At5g05470 [J] KOG2916 Translation initiation factor 2 alpha subunit
(eIF-2alpha)
Length = 344
Score = 251 bits (641), Expect = 9e-67
Identities = 138/309 (44%), Positives = 193/309 (61%), Gaps = 24/309 (7%)
Query: 6 CRFYENKFPEVDDVVMVNVQQIAEMGAYVRLLEYDNIEGMVXXXXXXXXXXXXXQKLIRV 65
CR YE+++P+VD VM+ V+ IA+MGAYV LLEY+NIEGM+ LI+V
Sbjct: 10 CRMYESRYPDVDMAVMIQVKTIADMGAYVSLLEYNNIEGMILFSELSRRRIRSISSLIKV 69
Query: 66 GKNEVVVVLRVDKEKGYIDLSKRRVSAEDVIKCEERYQKSKAVHSILRQCAEKYQVSLED 125
G+ E V+VLRVD+E+GYIDLSKRRVS ED CEERY KSK VHSI+R AE V LE+
Sbjct: 70 GRTEPVMVLRVDRERGYIDLSKRRVSDEDKEACEERYNKSKLVHSIMRHVAETVGVDLEE 129
Query: 126 LYKAYAWPLSRKYGHAYDAYKMSIVDPNVW-------------DGIE------SISDEIF 166
LY WPL +K+GHA++A+K+ + DP+ DG+E ++S+E+
Sbjct: 130 LYVNIGWPLYKKHGHAFEAFKIVVTDPDSVFDALTREVKETGPDGVEVTKVVPAVSEELK 189
Query: 167 QELKGQITRRLTPQAVKIRADVEVSCFSYEGIDAIKEALKSAEELSTEQMQIKVKLVAAP 226
I RR+TPQ +KIRAD+E+ CF ++G+ IKEA+K AE + T+ +K+KLVA P
Sbjct: 190 DAFLKDIRRRMTPQPMKIRADIELKCFQFDGVLHIKEAMKKAEAVGTDDCPVKIKLVAPP 249
Query: 227 LYVITTQSLDKDQGVQLLETAIAKIEEVILKYNGVCNVTMAPKAVTATED---AELQALL 283
LYV+TT + K++G+ L AI I ++ G V +AV+ +D AE A L
Sbjct: 250 LYVLTTHTHYKEKGIVTLNKAIEACITAIEEHKGKLVVKEGARAVSERDDKLLAEHMAKL 309
Query: 284 --ESKELEG 290
+++E+ G
Sbjct: 310 RMDNEEMSG 318
>CE29373 [J] KOG2916 Translation initiation factor 2 alpha subunit
(eIF-2alpha)
Length = 342
Score = 242 bits (618), Expect = 4e-64
Identities = 128/278 (46%), Positives = 184/278 (66%), Gaps = 8/278 (2%)
Query: 6 CRFYENKFPEVDDVVMVNVQQIAEMGAYVRLLEYDNIEGMVXXXXXXXXXXXXXQKLIRV 65
CRFYEN+FP+V++ V+ NV+ IA+MGAYVRL EY++ EGM+ KLIRV
Sbjct: 3 CRFYENQFPDVEETVVANVKMIADMGAYVRLSEYNDKEGMILLSELSRRRIRSVNKLIRV 62
Query: 66 GKNEVVVVLRVDKEKGYIDLSKRRVSAEDVIKCEERYQKSKAVHSILRQCAEKY----QV 121
G++E VVV+RVDK+KGYIDLSKRRV +D+ +C+ER+ +K V+SILR AE+
Sbjct: 63 GRSESVVVIRVDKDKGYIDLSKRRVYQKDLKQCDERFANAKMVNSILRHVAEQVGYTTDE 122
Query: 122 SLEDLYKAYAWPLSRK---YGHAYDAYKMSIVDPNVWDGIESISDEIFQELKGQITRRLT 178
LEDLY+ AW RK +YDA+K +I +P + D + IS +I ++L I ++LT
Sbjct: 123 ELEDLYQKTAWHFDRKEKRKAASYDAFKKAITEPTILDECD-ISADIKEKLLEDIRKKLT 181
Query: 179 PQAVKIRADVEVSCFSYEGIDAIKEALKSAEELSTEQMQIKVKLVAAPLYVITTQSLDKD 238
PQAVKIRAD+EVSCF Y+GIDA+K AL + + S IK+ L+AAP +V+TTQ+LD++
Sbjct: 182 PQAVKIRADIEVSCFDYDGIDAVKAALIAGKNCSNGTFPIKINLIAAPHFVVTTQTLDRE 241
Query: 239 QGVQLLETAIAKIEEVILKYNGVCNVTMAPKAVTATED 276
G++ + + + I+ I + G + + VT +D
Sbjct: 242 GGIEAVNSILDTIKNAIEGFKGKFTIKEEARIVTDIDD 279
>ECU11g0930i [J] KOG2916 Translation initiation factor 2 alpha subunit
(eIF-2alpha)
Length = 283
Score = 116 bits (290), Expect = 5e-26
Identities = 72/264 (27%), Positives = 127/264 (47%), Gaps = 8/264 (3%)
Query: 6 CRFYENKFPEVDDVVMVNVQQIAEMGAYVRLLEYDNIEGMVXXXXXXXXXXXXXQKLIRV 65
CR + K+P ++V+ V I G + LLEY ++EG+V Q++ +V
Sbjct: 6 CRLHSRKYPREGEIVIGRVVSIGGEGLTLNLLEYGDLEGLVLLGELSKRRVRSIQQVTKV 65
Query: 66 GKNEVVVVLRVDKEKGYIDLSKRRVSAEDVIKCEERYQKSKAVHSILRQCAEKYQVSLED 125
G E+ VL+VD+ +GYIDLS +V+ + +C E K+K + I+ + A++ + + +
Sbjct: 66 GNIEICNVLKVDEGRGYIDLSMSKVTENEKSECRETSAKNKLAYHIMVKAAKRLGMEVSE 125
Query: 126 LYKAYAWPLSRKYGHAYDAYKMSIVDPNVWDGIESISDEIFQELKGQITRRLTPQAVKIR 185
LY+ + ++G Y + + ++ D D I +K I + K+R
Sbjct: 126 LYERMGYDKEEEFGSLYYFFARVKDNGDIMD-----DDAIGVTIKKLIREQFQASTYKVR 180
Query: 186 ADVEVSCFSYEGIDAIKEALKSAEELSTEQMQIKVKLVAAPLYVITTQSLDKDQGVQLLE 245
AD++V C S GI +IK+ A L +++ L+ P Y +T S +KD+ +++
Sbjct: 181 ADIDVVCSSRGGIVSIKKVFSDALRLDP---RLEFCLIKTPTYSVTRVSSNKDKAYEVVR 237
Query: 246 TAIAKIEEVILKYNGVCNVTMAPK 269
A + I K G V PK
Sbjct: 238 EACNVLISNIQKEGGTATVISQPK 261
>At2g40290 [J] KOG2916 Translation initiation factor 2 alpha subunit
(eIF-2alpha)
Length = 154
Score = 114 bits (286), Expect = 1e-25
Identities = 56/134 (41%), Positives = 84/134 (61%), Gaps = 19/134 (14%)
Query: 123 LEDLYKAYAWPLSRKYGHAYDAYKMSIVDPN-------------------VWDGIESISD 163
L+DLY WPL R++GHA++A+K+ + DP+ V + ++++
Sbjct: 2 LQDLYVNIGWPLYRRHGHAFEAFKILVTDPDSVLGPLTREIKEVGPDGQEVTKVVPAVTE 61
Query: 164 EIFQELKGQITRRLTPQAVKIRADVEVSCFSYEGIDAIKEALKSAEELSTEQMQIKVKLV 223
E+ L I RR+TPQ +KIRAD+E+ CF ++G+ IKEA+K+AE E +K+KLV
Sbjct: 62 EVKDALVKNIRRRMTPQPMKIRADIELKCFQFDGVVHIKEAMKNAEAAGNEDCPVKIKLV 121
Query: 224 AAPLYVITTQSLDK 237
A PLYV+TTQ+LDK
Sbjct: 122 APPLYVLTTQTLDK 135
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.132 0.361
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,234,334
Number of Sequences: 60738
Number of extensions: 517730
Number of successful extensions: 1664
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1643
Number of HSP's gapped (non-prelim): 8
length of query: 302
length of database: 30,389,216
effective HSP length: 106
effective length of query: 196
effective length of database: 23,950,988
effective search space: 4694393648
effective search space used: 4694393648
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)