ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactI3054 good R KOG3086 General function prediction only Predicted dioxygenase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactI3054 1023063 1024025 321
(321 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJR008w [R] KOG3086 Predicted dioxygenase 309 4e-84
SPAC4H3.04c [R] KOG3086 Predicted dioxygenase 203 3e-52
At2g25280 [R] KOG3086 Predicted dioxygenase 196 5e-50
7299707 [R] KOG3086 Predicted dioxygenase 191 9e-49
Hs7705720 [R] KOG3086 Predicted dioxygenase 188 1e-47
Hs13646632 [R] KOG3086 Predicted dioxygenase 171 2e-42
CE24833 [R] KOG3086 Predicted dioxygenase 164 1e-40
Hs13641722 [R] KOG3086 Predicted dioxygenase 70 3e-12
>YJR008w [R] KOG3086 Predicted dioxygenase
Length = 338
Score = 309 bits (791), Expect = 4e-84
Identities = 162/335 (48%), Positives = 213/335 (63%), Gaps = 19/335 (5%)
Query: 3 SVRPATHAGSWYSSSPALITTLQQLLSRDEAVPGTKH----LISPHAGYKYCGDTMALGY 58
S+RPATHAGSWYS+ ++ + G H +I PHAGY+YCG TMA Y
Sbjct: 2 SIRPATHAGSWYSNRAQELSQQLHTYLIKSTLKGPIHNARIIICPHAGYRYCGPTMAYSY 61
Query: 59 SKLDFT-NVKRCIIMGPSHHIYFKNKIQVTSFDEVNTPFGDFKVDGGLRNELLV-----G 112
+ LD NVKR I+GPSHHIYFKN+I V++F E+ TP G+ KVD L L+
Sbjct: 62 ASLDLNRNVKRIFILGPSHHIYFKNQILVSAFSELETPLGNLKVDTDLCKTLIQKEYPEN 121
Query: 113 GKVVL--MSKEVDMDEHSLEMQFPMLWAAAKLRGVDPTNIKILPLLVSHNSTKVDFELGK 170
GK + M + DM EHSLEMQ PML K R + +K+ P++VSHNS VD +G
Sbjct: 122 GKKLFKPMDHDTDMAEHSLEMQLPMLVETLKWREISLDTVKVFPMMVSHNSVDVDRCIGN 181
Query: 171 LLQKYVDDEETLFILSSDFCHWGRRFGYTGYVMESSDIDESLENDSEVEYLTQRSFNHKS 230
+L +Y+ D LFI+SSDFCHWGRRF YTGYV +++++++ ++EVE LT RS S
Sbjct: 182 ILSEYIKDPNNLFIVSSDFCHWGRRFQYTGYVGSKEELNDAIQEETEVEMLTARS--KLS 239
Query: 231 SHNVPIYKSIEILDQYAMDILGKNTENKYYD-WKKYINVTGNTICGERPIGVFLCAMSLL 289
H VPI++SIEI+D+YAM L + YD WK+Y+ +TGNTICGE+PI V L A+S +
Sbjct: 240 HHQVPIWQSIEIMDRYAMKTLSDTPNGERYDAWKQYLEITGNTICGEKPISVILSALSKI 299
Query: 290 N----KPYQFNWAHYSQSSQCVDLEDSSVSYASGY 320
+F W +YSQSS ++DSSVSYASGY
Sbjct: 300 RDAGPSGIKFQWPNYSQSSHVTSIDDSSVSYASGY 334
>SPAC4H3.04c [R] KOG3086 Predicted dioxygenase
Length = 309
Score = 203 bits (516), Expect = 3e-52
Identities = 117/319 (36%), Positives = 176/319 (54%), Gaps = 19/319 (5%)
Query: 3 SVRPATHAGSWYSSSPALITT-LQQLLSRDEAVPGTKHLISPHAGYKYCGDTMALGYSKL 61
++R ATHAGSWY L+T L+ + G + +ISPHAGY Y G + G+ +L
Sbjct: 4 AIREATHAGSWYLDDTELLTKQLKSFIKNPTPETGKRFVISPHAGYMYSGKVASQGFQQL 63
Query: 62 DFTNVKRCIIMGPSHHIYFKNKIQVTSFDEVNTPFGDFKVDGGLRNELLVGGKVV-LMSK 120
DF+ ++R + GPSHHI F K V+ +TP GD KVD L +L+ M+
Sbjct: 64 DFSKIQRVFVFGPSHHI-FTRKCLVSRASICSTPLGDLKVDEDLCQKLVASDNSFDSMTL 122
Query: 121 EVDMDEHSLEMQFPMLWAAAKLRGVDPTNIKILPLLVSHNSTKVDFELGKLLQKYVDDEE 180
+VD EHSLEMQFP+L A L+ +KI+P+++ ++ K L +Y+ DE
Sbjct: 123 DVDESEHSLEMQFPLL-AFHLLKQGCLGKVKIVPIMIGALTSTTMMAAAKFLSQYIKDES 181
Query: 181 TLFILSSDFCHWGRRFGYTGYVMESSDIDESLENDSEVEYLTQRSFNHKSSHNVP-IYKS 239
F++SSDFCHWGRRFGYT Y+ +++ +++++ + + P IY+S
Sbjct: 182 NSFVISSDFCHWGRRFGYTLYLNDTNQLEDAV-----------LKYKRRGGPTSPKIYES 230
Query: 240 IEILDQYAMDILGKNTENKYYDWKKYINVTGNTICGERPIGVFLCAMSLLNKPYQFNWAH 299
I LD M I+ + + D+ +Y+ T NTICG PI + + +M N +F +
Sbjct: 231 ISNLDHIGMKIIETKSSD---DFSEYLKTTQNTICGRYPIELIMKSMECANFSERFKFIS 287
Query: 300 YSQSSQCVDLEDSSVSYAS 318
Y+QSS + DSSVSYA+
Sbjct: 288 YAQSSHVELVTDSSVSYAT 306
>At2g25280 [R] KOG3086 Predicted dioxygenase
Length = 291
Score = 196 bits (497), Expect = 5e-50
Identities = 118/321 (36%), Positives = 169/321 (51%), Gaps = 40/321 (12%)
Query: 1 MSSVRPATHAGSWYSSSPALITT-LQQLLSRDEAV--PGTKHLISPHAGYKYCGDTMALG 57
M +R THAGSWY+ +P +++ L++ L+ P + +I+PHAGY Y G A
Sbjct: 1 MEKIRQPTHAGSWYTDNPTKLSSDLEEWLNATGLTKSPDVRGVIAPHAGYSYSGRAAAYA 60
Query: 58 YSKLDFTNVKRCIIMGPSHHIYFKNKIQVTSFDEVNTPFGDFKVDGGLRNELLVGGKVVL 117
++ +D TN+ R ++GPSHH Y K +++ TP G+ VD + E+ GK +
Sbjct: 61 FANIDPTNISRIFLLGPSHHFYTP-KCALSTATVYKTPIGNLPVDVEMIKEIRAMGKFGM 119
Query: 118 MSKEVDMDEHSLEMQFPMLWAAAKLRGVDPTNIKILPLLVSHNSTKVDFELGKLLQKYVD 177
M VD EHS+EM P L AK+ + N+K++P+LV S + + G+LL KYVD
Sbjct: 120 MDLRVDEAEHSMEMHLPYL---AKV--FEGNNVKVVPILVGAVSPENEAMYGELLAKYVD 174
Query: 178 DEETLFILSSDFCHWGRRFGYTGYVMESSDIDESLENDSEVEYLTQRSFNHKSSHNVPIY 237
D + F +SSDFCHWG RF Y Y ++H I+
Sbjct: 175 DPKNFFSVSSDFCHWGSRFNYMHY---------------------------DNTHGA-IH 206
Query: 238 KSIEILDQYAMDILGKNTENKYYDWKKYINVTGNTICGERPIGVFLCAMSLLNKPYQFNW 297
KSIE LD+ MDI+ + + KKY+ NTICG PI +FL + + + N+
Sbjct: 207 KSIEALDKKGMDIIETGDPDAF---KKYLLEFENTICGRHPISIFLHMLKHSSSKIKINF 263
Query: 298 AHYSQSSQCVDLEDSSVSYAS 318
Y QSSQC + DSSVSYAS
Sbjct: 264 LRYEQSSQCQTMRDSSVSYAS 284
>7299707 [R] KOG3086 Predicted dioxygenase
Length = 295
Score = 191 bits (486), Expect = 9e-49
Identities = 122/323 (37%), Positives = 163/323 (49%), Gaps = 41/323 (12%)
Query: 3 SVRPATHAGSWYSSSPA-LITTLQQLLSRDEAVPG-TKHLISPHAGYKYCGDTMALGYSK 60
S R ATHAGSWY+ S A L L + L + G + +I+PHAGY YCG A Y +
Sbjct: 2 SARRATHAGSWYTDSGAELSRQLDRWLGAADLSHGPARAIIAPHAGYTYCGACAAFAYRQ 61
Query: 61 LDFTNVKRCIIMGPSHHIYFKNKIQVTSFDEVNTPFGDFKVDGGLRNELLVGGKVVLMSK 120
+ VKR I+GPSHH+ + ++ + TP D K+D + +EL GK M
Sbjct: 62 VSPVVVKRIFILGPSHHVRLRG-CALSVAKKYRTPLYDLKIDAQINSELEKTGKFSWMDM 120
Query: 121 EVDMDEHSLEMQFPMLWAAAKLRGVDPTNIKILPLLVSHNSTKVDFELGKLLQKYVDDEE 180
+ D DEHS+EM P + AK+ I+P+LV + + + + G LL Y+ D
Sbjct: 121 KTDEDEHSIEMHLPYI---AKVMEDYKDQFTIVPILVGSLNPEQEAQYGSLLSSYLMDPT 177
Query: 181 TLFILSSDFCHWGRRFGYTGYVMESSDIDESLENDSEVEYLTQRSFNHKSSHNVPIYKSI 240
LF++SSDFCHWG RF YT Y S I+KSI
Sbjct: 178 NLFVISSDFCHWGHRFSYTYY----------------------------DSSCGAIHKSI 209
Query: 241 EILDQYAMDILGKNTENKYYDWKKYINVTGNTICGERPIGVFLCAMSLLN----KPYQFN 296
E LD+ MDI+ + + +Y+ NTICG PIGV L A+ L F
Sbjct: 210 EKLDKQGMDIIESLNPHSF---TEYLRKYNNTICGRHPIGVMLGAVKALQDQGYDKMSFK 266
Query: 297 WAHYSQSSQCVDLEDSSVSYASG 319
+ Y+QSSQC D+EDSSVSYASG
Sbjct: 267 FLKYAQSSQCQDIEDSSVSYASG 289
>Hs7705720 [R] KOG3086 Predicted dioxygenase
Length = 297
Score = 188 bits (477), Expect = 1e-47
Identities = 121/320 (37%), Positives = 169/320 (52%), Gaps = 40/320 (12%)
Query: 5 RPATHAGSWYSSS-PALITTLQQLLSRDEAVPG-TKHLISPHAGYKYCGDTMALGYSKLD 62
R A+HAGSWY++S P L L+ LS+ ++ + +I+PHAGY YCG A Y ++D
Sbjct: 8 REASHAGSWYTASGPQLNAQLEGWLSQVQSTKRPARAIIAPHAGYTYCGSCAAHAYKQVD 67
Query: 63 FTNVKRCIIMGPSHHIYFKNKIQVTSFDEVNTPFGDFKVDGGLRNELLVGGKVVLMSKEV 122
+ +R I+GPSHH+ ++ ++S D TP D ++D + EL G MS +
Sbjct: 68 PSITRRIFILGPSHHVPL-SRCALSSVDIYRTPLYDLRIDQKIYGELWKTGMFERMSLQT 126
Query: 123 DMDEHSLEMQFPMLWAAAKLRGVDPTNIKILPLLVSHNSTKVDFELGKLLQKYVDDEETL 182
D DEHS+EM P AK I+P+LV S + E GKL KY+ D L
Sbjct: 127 DEDEHSIEMHLPY---TAKAMESHKDEFTIIPVLVGALSESKEQEFGKLFSKYLADPSNL 183
Query: 183 FILSSDFCHWGRRFGYTGYVMESSDIDESLENDSEVEYLTQRSFNHKSSHNVPIYKSIEI 242
F++SSDFCHWG+RF Y+ Y DES IY+SIE
Sbjct: 184 FVVSSDFCHWGQRFRYSYY-------DES---------------------QGEIYRSIEH 215
Query: 243 LDQYAMDILGKNTENKYYDWKKYINVTGNTICGERPIGVFLCAMSLLNK---PYQFNWAH 299
LD+ M I+ + + Y+ NTICG PIGV L A++ L K F++ +
Sbjct: 216 LDKMGMSII---EQLDPVSFSNYLKKYHNTICGRHPIGVLLNAITELQKNGMNMSFSFLN 272
Query: 300 YSQSSQCVDLEDSSVSYASG 319
Y+QSSQC + +DSSVSYA+G
Sbjct: 273 YAQSSQCRNWQDSSVSYAAG 292
>Hs13646632 [R] KOG3086 Predicted dioxygenase
Length = 295
Score = 171 bits (432), Expect = 2e-42
Identities = 117/320 (36%), Positives = 165/320 (51%), Gaps = 42/320 (13%)
Query: 5 RPATHAGSWYSSS-PALITTLQQLLSRDEAVPG-TKHLISPHAGYKYCGDTMALGYSKLD 62
R A+HAGSWY++S P L L+ LS+ ++ + +I+PHAGY YCG A Y ++D
Sbjct: 8 REASHAGSWYTASGPQLNAQLEGWLSQVQSTKRPARAIIAPHAGYTYCGSCAAHAYKQVD 67
Query: 63 FTNVKRCIIMGPSHHIYFKNKIQVTSFDEVNTPFGDFKVDGGLRNELLVGGKVVLMSKEV 122
+ +R I+GPSHH+ ++ ++S D TP D ++D + EL G MS +
Sbjct: 68 PSITRRIFILGPSHHVPL-SRCALSSVDIYRTPLYDLRIDQKIYGELWKTGMFERMSLQT 126
Query: 123 DMDEHSLEMQFPMLWAAAKLRGVDPTNIKILPLLVSHNSTKVDFELGKLLQKYVDDEETL 182
D DE S+EM P AK I+PLLV S K +L KY+ D L
Sbjct: 127 DEDERSIEMHLPY---TAKAMESHKDEFTIIPLLVGALSQK--NRNSELFSKYLADPSNL 181
Query: 183 FILSSDFCHWGRRFGYTGYVMESSDIDESLENDSEVEYLTQRSFNHKSSHNVPIYKSIEI 242
F++SSDFCHWG+RF ++ Y DES I +SIE
Sbjct: 182 FVVSSDFCHWGQRFRHSYY-------DES---------------------QGEICRSIEH 213
Query: 243 LDQYAMDILGKNTENKYYDWKKYINVTGNTICGERPIGVFLCAMSLLNK---PYQFNWAH 299
LD+ M I+ + + Y+ NTICG PIGV L A++ L K F++ +
Sbjct: 214 LDKMGMSII---EQLDPVSFSNYLKKYHNTICGRHPIGVLLNAITELQKNGMNMSFSFLN 270
Query: 300 YSQSSQCVDLEDSSVSYASG 319
Y+QSSQC + +DSSVSYA+G
Sbjct: 271 YAQSSQCRNWQDSSVSYAAG 290
>CE24833 [R] KOG3086 Predicted dioxygenase
Length = 392
Score = 164 bits (416), Expect = 1e-40
Identities = 112/324 (34%), Positives = 160/324 (48%), Gaps = 48/324 (14%)
Query: 5 RPATHAGSWYSSSPALITTLQQLLSR--DEAVP--GT-KHLISPHAGYKYCGDTMALGYS 59
R A+HAGSWY+++ L + L++ D A P GT + LISPHAGY YCG+T A +
Sbjct: 101 RSASHAGSWYNANQR---DLDRQLTKWLDNAGPRIGTARALISPHAGYSYCGETAAYAFK 157
Query: 60 KLDFTNVKRCIIMGPSHHIYFKNKIQVTSFDEVNTPFGDFKVDGGLRNELLVGGKVVLMS 119
++ + V+R I+GPSH + N +T+ + TP GD VD + EL LM
Sbjct: 158 QVVSSAVERVFILGPSHVVAL-NGCAITTCSKYRTPLGDLIVDHKINEELRATRHFDLMD 216
Query: 120 KEVDMDEHSLEMQFPMLWAAAKLRGVDPTNIKILPLLVSHNSTKVDFELGKLLQKYVDDE 179
+ + EHS+EMQ P + AK+ G I+P+LV G + Y++D
Sbjct: 217 RRDEESEHSIEMQLPFI---AKVMG--SKRYTIVPVLVGSLPGSRQQTYGNIFAHYMEDP 271
Query: 180 ETLFILSSDFCHWGRRFGYTGYVMESSDIDESLENDSEVEYLTQRSFNHKSSHNVPIYKS 239
LF++SSDFCHWG RF ++ Y SS +PIY+
Sbjct: 272 RNLFVISSDFCHWGERFSFSPYDRHSS---------------------------IPIYEQ 304
Query: 240 IEILDQYAMDILGKNTENKYYDWKKYINVTGNTICGERPIGVFLCAMSLL----NKPYQF 295
I +D+ M + + D Y+ T NTICG PI + L A N ++F
Sbjct: 305 ITNMDKQGMSAIETLNPAAFND---YLKKTQNTICGRNPILIMLQAAEHFRISNNHTHEF 361
Query: 296 NWAHYSQSSQCVDLEDSSVSYASG 319
+ HY+QS++ DSSVSYASG
Sbjct: 362 RFLHYTQSNKVRSSVDSSVSYASG 385
>Hs13641722 [R] KOG3086 Predicted dioxygenase
Length = 159
Score = 70.5 bits (171), Expect = 3e-12
Identities = 40/121 (33%), Positives = 67/121 (55%), Gaps = 3/121 (2%)
Query: 7 ATHAGSWYSSS-PALITTLQQLLSRDEAVPG-TKHLISPHAGYKYCGDTMALGYSKLDFT 64
A+H+G+WY++S P L L+ LS+ ++ + +I+PHAGY YCG Y ++D +
Sbjct: 10 ASHSGNWYTASGPQLNAQLEGWLSQVKSTKRPARAIIAPHAGYTYCGSCATHAYKQVDPS 69
Query: 65 NVKRCIIMGPSHHIYFKNKIQVTSFDEVNTPFGDFKVDGGLRNELLVGGKVVLMSKEVDM 124
+ I+GPSHH+ ++ ++S D TP D ++D + EL G MS + M
Sbjct: 70 ITQGIFILGPSHHVSL-SQCALSSVDIYRTPLHDLRIDQKIYRELWKTGMFERMSLQTKM 128
Query: 125 D 125
+
Sbjct: 129 N 129
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.135 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,294,024
Number of Sequences: 60738
Number of extensions: 891683
Number of successful extensions: 2088
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2041
Number of HSP's gapped (non-prelim): 11
length of query: 321
length of database: 30,389,216
effective HSP length: 106
effective length of query: 215
effective length of database: 23,950,988
effective search space: 5149462420
effective search space used: 5149462420
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)