ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII0203 good U KOG2273 Intracellular trafficking, secretion, and vesicular transport Membrane coat complex Retromer, subunit VPS5/SNX1, Sorting nexins, and related PX domain-containing proteins
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII0203 62255 63979 575
(575 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR425w [U] KOG2273 Membrane coat complex Retromer subunit VPS5/... 280 3e-75
SPBC14F5.11c [U] KOG2273 Membrane coat complex Retromer subunit ... 100 8e-21
YDL113c [U] KOG2273 Membrane coat complex Retromer subunit VPS5/... 79 2e-14
>YDR425w [U] KOG2273 Membrane coat complex Retromer subunit VPS5/SNX1
Sorting nexins and related PX domain-containing proteins
Length = 625
Score = 280 bits (717), Expect = 3e-75
Identities = 188/608 (30%), Positives = 301/608 (48%), Gaps = 51/608 (8%)
Query: 9 EEDNNPFSGTTHLYASGIAA-VTDGPDDYDFTEPSING--SSDENAQSNAVXXXXXXXXX 65
EEDNNPF GT HLYASGI + + + + + PS + SS ++ S
Sbjct: 23 EEDNNPFVGTHHLYASGIGTTIGEARPENENSPPSSSSLPSSPAHSSSAGSSRASTSSST 82
Query: 66 XXXXXXXXXLQTWRFASAELSRSSAFETSYTNLLGQGKNPEVIRIVDAGQYRDIYGKYAI 125
+T F S +S ++ + + + I+I+DAG ++D +GK+AI
Sbjct: 83 SSHAVVEADAETEPFVSLSMSTTATISKFTPHDMN---GTQQIQIIDAGDFKDPWGKHAI 139
Query: 126 GYKIEFGGIVVTRRYSEFDSLRQSLCRLLXXXXXXXXXXXXXXXKYLFNPLHAKKDIKII 185
GY I + + RRYSEF SLRQSL RLL KY+++P++A D KII
Sbjct: 140 GYVILYENNKIIRRYSEFHSLRQSLTRLLPTIIIPPIPSKHSLLKYIWSPINAANDSKII 199
Query: 186 ERRQRLLSRFLNNCHKVREIRNHIVFQKFLNPEYFWKEVLNTPPISIXXXXXXXXXXXXX 245
R+++L+ FL+NC ++EI N IVFQKFLNPE+ WK+VL++ PI I
Sbjct: 200 STRKKMLNSFLSNCLNIQEISNDIVFQKFLNPEFNWKDVLSSSPIIILPLNNLLAPPLSP 259
Query: 246 TKPSPIHLLLPTPTVLTMR-------KHEQLIGRNDVM---------EIKFADYDSDLIR 289
TKPSP+H +LP P+ ++R +H + N++ E +F ++
Sbjct: 260 TKPSPLHSILPIPSNSSLRNYNSIWQQHITVKSHNEISNLPTEILQNESQFTHIENLFQN 319
Query: 290 YKAILQPLNKTVRSIRSNIQTYSAVLSELGAYFNAFSLENSVFQVSALFEQMN------- 342
YK I+ L K +RS +S+ + S +ELGAY+NAFSLEN + ++L E N
Sbjct: 320 YKRIITHLLKNIRSNKSHFHSLSTYFAELGAYYNAFSLENDITMPNSLRESENNSNNPMM 379
Query: 343 RLSMGIEKTGQAIDVNYVSAEIFSEAIMISLEEGSKEMLQFIHEAQRVLHFRNFKQEQFY 402
+ IEKTG + DV Y+S+EI E LE+ E+LQF++E+ +VL+F+ K QF
Sbjct: 380 EIISHIEKTGHSFDVIYISSEILIEKYTSILEDPINELLQFLNESFKVLNFKKLKFLQFK 439
Query: 403 TIETTIKKRKDRIRELKEADLQAVRLGEALKLNAEESPTVAQVMDSMTRKSANKNHTDKQ 462
+E I +++ ++ L E + Q ++ E+L + T+ + K+A+ K
Sbjct: 440 ILERLIIEKETKLSSLTEIENQLQKINESLTRS-----TILTDENYKDTKAADLTFVKKD 494
Query: 463 IMGLFRSSASPNNKSGSDSIS-----------------SEVEPHLLTKDERVVQVNXXXX 505
+ L +SS++ ++ + I +++E LT+ ER Q+
Sbjct: 495 VRSLSKSSSNSSSSGHQNEIHIGASKLNYKTSTPTMNLNKLEIKQLTEQERSKQIKQLNQ 554
Query: 506 XXXXXXXXXXXIEKDLQQVNESMDNSLNNLEKYFHEKWFLIFRELAHNITSWLKDCSESW 565
D+ ++N S NSL + + + I + A + +W+K+C ++W
Sbjct: 555 DLSKLKDCLSICISDMLEINNSSYNSLMHTYNHINLTIGKILKLFAASFKAWIKECLKNW 614
Query: 566 KNAKQSID 573
K AK ID
Sbjct: 615 KLAKLQID 622
>SPBC14F5.11c [U] KOG2273 Membrane coat complex Retromer subunit VPS5/SNX1
Sorting nexins and related PX domain-containing proteins
Length = 586
Score = 100 bits (248), Expect = 8e-21
Identities = 114/457 (24%), Positives = 183/457 (39%), Gaps = 65/457 (14%)
Query: 10 EDNNPFSGTTHLYASGIAAVTDGPDDYDFTEPSINGSSDENAQS---NAVXXXXXXXXXX 66
EDNNPFSG+ + AS V EP + S + + S NA+
Sbjct: 5 EDNNPFSGSDNRSASSAVNV----------EPKVEPSQHQGSSSVKENAISQPNESFQ-- 52
Query: 67 XXXXXXXXLQTWRFASAELSRSSAFETSYTNLLGQGKNPEVIRIVDAGQYRDIYGKYAIG 126
SR+ F+ +++ +P IV G + G + +
Sbjct: 53 -------------------SRNMFFQKDVDSVVDSALDPN--GIVITGAMKAESGSHIV- 90
Query: 127 YKIEFGGIVVTRRYSEFDSLRQSLCRLLXXXXXXXXXXXXXXXKYLFNPLHAKKDIKIIE 186
Y I+ + RYSEF SLR L RL YL N ++ +++E
Sbjct: 91 YIIKLQDSEIHHRYSEFASLRVQLSRLYPTCLVPPLPDKHKIMDYLINVTKNQRMSRMLE 150
Query: 187 RRQRLLSRFLNNCHKVREIRNHIVFQKFLNPEYFWKEVLNTPPISIXXXXXXXX--XXXX 244
R+RLL FL + + VF+KFL+ WKEVL++PPIS
Sbjct: 151 ERKRLLQLFLRRVAQHPILGLSEVFRKFLSRHVSWKEVLHSPPISCLPKDLLKAPPADPS 210
Query: 245 XTKPSPIHLLLPTPT-VLTMRKHEQLIGRNDVMEIKFADYDSDLIRYKAILQPLNKTVRS 303
+ + ++ LP P+ L R + +G+N +M + L +Y + Q +
Sbjct: 211 SKENAELYKELPIPSKTLVPRDNYDDVGKNFLM------LEDTLQQYSIVAQEESNLFNQ 264
Query: 304 IRSNIQTYSAVLSELGAYFNAFSLENSVFQVSALFEQMNRLSMGIEKTGQAIDVNYVSAE 363
I + Y S LGA FNA SL S +L +EK GQA D +++
Sbjct: 265 IVLSNSKYCLAHSTLGAMFNALSLSES-----------GKLLTALEKVGQANDHTCLASI 313
Query: 364 IFSEAIMISLEEGSKEMLQFIHEAQRVLHFRNFKQEQFYTIETTIKKRKDRIRELKEADL 423
F +I++ E +E+ + + + FR K Q +E + ++K + L+ +
Sbjct: 314 DFMHNFVIAVIEPLQELSKDAKNMRHIFIFRKMKFIQQVMVEELLTRKKSFLHLLERRER 373
Query: 424 QAVRLGEALK-------LNAEESPTVAQVMDSMTRKS 453
A RL +A+ LN E T+ V ++ T +S
Sbjct: 374 HAARLQQAIGEVDGDVILNRESEATLG-VNNAQTSRS 409
>YDL113c [U] KOG2273 Membrane coat complex Retromer subunit VPS5/SNX1
Sorting nexins and related PX domain-containing proteins
Length = 640
Score = 79.0 bits (193), Expect = 2e-14
Identities = 104/464 (22%), Positives = 182/464 (38%), Gaps = 73/464 (15%)
Query: 108 IRIVDAGQYRDIYGKYAIGYKIEFGGIVVTRRYSEFDSLRQSLCRLL-----------XX 156
I I++A + + G+ I Y I+F V RRYS+F+SLR L RL
Sbjct: 161 IHILEAKRVSEGQGRAYIAYVIQFENSTVQRRYSDFESLRSILIRLFPMTLIPPIPEKQS 220
Query: 157 XXXXXXXXXXXXXKYLF------------NPLHAK---KDIKIIERRQRLLSRFLNNCHK 201
KYL + +HA D K+I R R+L+ FLN
Sbjct: 221 IKNYGKSITGSSSKYLLPSEGSGSVDLSLSVIHASVNNSDEKLIRHRIRMLTEFLNKLLT 280
Query: 202 VREIRNHIVFQKFLNP-EYFWKEVLN-TPPISIXXXXXXXXXXXXXTKPSPIHLLLPTP- 258
EI + FL+P + W E +N + S T + IH +LP P
Sbjct: 281 NEEITKTSIITDFLDPNNHNWHEFVNSSSTFSSLPKSILQCNPLDPTNTTRIHAMLPIPG 340
Query: 259 --TVLTMRKHEQLIGRNDVMEIKFADYDSDLIRYKAILQPLNKTVRSIRSNIQTYSAVLS 316
+ L + K + F + + D +Y+ +L N + R +TY + S
Sbjct: 341 SSSQLLLNKESNDKKMDKERSKSFTNIEQDYKQYENLLD--NGIYKYNRRTTKTYHDLKS 398
Query: 317 ---ELGAYFNAFSLENSVFQVSALFEQMNRLSMGIEKTGQAIDVNYVSAEIFSEAIMISL 373
E+G F F+ E + QV L EQ++ LS A + +S E + ++
Sbjct: 399 DYNEIGEVFAQFAHEQA--QVGELAEQLSYLS-------NAFSGSSISLEKLVGRLYYNI 449
Query: 374 EEGSKEMLQFIHEAQRVLHFRNFKQEQFYTIETTIKKRKDRIRELKEADLQAVRLGEALK 433
E E + A+ ++ +R K Q I+ ++ ++ ++ +L+ + + + + +
Sbjct: 450 NEPLNESVHMATSARELIKYRKLKYLQNEMIKKSLNSKRAQLEKLEAQNNEYKDVDKIID 509
Query: 434 LNAEESPTVAQVMDSMTRKSANKNHTDKQIMGLFRSSASPNNKSGSDSIS-SEVEPHLLT 492
+S T+ + S K++ K G F AS DS+ E +PH
Sbjct: 510 NEMSKSHTINLERPNNNTGSGGKSYGGKLFNG-FNKLAS----MVKDSVKYQETDPH--- 561
Query: 493 KDERVVQVNXXXXXXXXXXXXXXXIEKDLQQVNESMDNSLNNLE 536
++K+++Q++ES++ + N+LE
Sbjct: 562 -------------------TASINLKKEIEQLSESLEVTENDLE 586
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.133 0.372
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,555,295
Number of Sequences: 60738
Number of extensions: 1115885
Number of successful extensions: 3371
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 3358
Number of HSP's gapped (non-prelim): 5
length of query: 575
length of database: 30,389,216
effective HSP length: 112
effective length of query: 463
effective length of database: 23,586,560
effective search space: 10920577280
effective search space used: 10920577280
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)