ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII0205 good O KOG2908 Posttranslational modification, protein turnover, chaperones 26S proteasome regulatory complex, subunit RPN9/PSMD13
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII0205 64284 65465 394
(394 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR427w [O] KOG2908 26S proteasome regulatory complex subunit RP... 493 e-139
SPAC607.05 [O] KOG2908 26S proteasome regulatory complex subunit... 230 3e-60
7301037 [O] KOG2908 26S proteasome regulatory complex subunit RP... 203 3e-52
Hs4506223 [O] KOG2908 26S proteasome regulatory complex subunit ... 185 9e-47
Hs14774537 [O] KOG2908 26S proteasome regulatory complex subunit... 185 9e-47
At5g45620 [O] KOG2908 26S proteasome regulatory complex subunit ... 160 2e-39
CE02329 [O] KOG2908 26S proteasome regulatory complex subunit RP... 152 1e-36
>YDR427w [O] KOG2908 26S proteasome regulatory complex subunit RPN9/PSMD13
Length = 393
Score = 493 bits (1268), Expect = e-139
Identities = 245/392 (62%), Positives = 303/392 (76%), Gaps = 3/392 (0%)
Query: 3 HEIDTILSTLKQEVNSELAPLFDDFEDLYERKLWHQLTEKLAIFFHDSKSESFRLRLYTQ 62
HEIDTILSTL+ E + L PLF+ FE YE KLW QL+E L FF D+KS RLRLY
Sbjct: 5 HEIDTILSTLRMEADPSLHPLFEQFEKFYEEKLWFQLSESLTKFFDDAKSTPLRLRLYDN 64
Query: 63 FVSKFIDKINQLNVVEFLLQALQENDDTEESLQYLKDLQKEFKEIDEKKQRNDGLKPHVN 122
FVSKF DKINQL+VV++LL +L+++ D +ESL+YL DL+ +F+E+D KKQRN+G K H +
Sbjct: 65 FVSKFYDKINQLSVVKYLLASLKDSKDFDESLKYLDDLKAQFQELDSKKQRNNGSKDHGD 124
Query: 123 GNLLLDIEIARVKLEKGDLVEVRDILDAIGKTLDVQDSVPLRVTGAYYSCNASYYQVKKD 182
G LL+D EIAR L K DLV+ RD+LD + KTLD +DS+PLR+T ++YS N+ Y++ K D
Sbjct: 125 GILLIDSEIARTYLLKNDLVKARDLLDDLEKTLDKKDSIPLRITNSFYSTNSQYFKFKND 184
Query: 183 FNNFYYTSLLYLSTITSXXXXXXXXXXXXXXXAYNLCIAALLGDNIYNFGELLQHPIISS 242
FN+FYYTSLLYLST+ AY+L I+ALLGD IYNFGELL HPI+ +
Sbjct: 185 FNSFYYTSLLYLSTLEPSTSITLAERQQL---AYDLSISALLGDKIYNFGELLHHPIMET 241
Query: 243 ISNDKEYEWLFRFLNALTEGDFQKFDQISKERIPKVPILAQHESFLRQKICLMTLVESVF 302
I ND Y+WLF+ LNALT GDF KFD + K +I K+PILAQHESFLRQKICLMTL+E+VF
Sbjct: 242 IVNDSNYDWLFQLLNALTVGDFDKFDSLIKVQISKIPILAQHESFLRQKICLMTLIETVF 301
Query: 303 AKSIRTLSFRDIADATFLPIDSVEHLVMRSISLGLLKGSIDQVQQLVTITWVQPRIINQE 362
K+IR LSF DI+ AT LP D+VEHLVMR+ISLGLLKGSIDQV +LVTI+WVQPRII+ +
Sbjct: 302 VKNIRMLSFEDISKATHLPKDNVEHLVMRAISLGLLKGSIDQVNELVTISWVQPRIISGD 361
Query: 363 QIKKMKDNLIKWQDEVSKLGNKIEGRGKSIWV 394
QI KMKD L++W D+V KLG K+E RG+SIWV
Sbjct: 362 QITKMKDRLVEWNDQVEKLGKKMEARGQSIWV 393
>SPAC607.05 [O] KOG2908 26S proteasome regulatory complex subunit RPN9/PSMD13
Length = 381
Score = 230 bits (586), Expect = 3e-60
Identities = 145/369 (39%), Positives = 208/369 (56%), Gaps = 21/369 (5%)
Query: 28 EDLYERKLWHQLTEKLAIFFHDSKSESFRLRLYTQFVSKFIDKINQLNVVEFLLQALQEN 87
EDLYERKLW QLT+ L +FF ++ RL LYT FV+ F INQL V L+A +
Sbjct: 32 EDLYERKLWKQLTDALIVFFDTPETVPLRLPLYTNFVNSFRPNINQLKAVYMGLKAFESC 91
Query: 88 DDTEESLQYLKDLQKEFKEIDEKKQRNDGLKPHVNGNLLLDIEIARVKLEKGDLVEVRDI 147
+ E L++L + E+DE+K ++ + + IAR+KL G L E R++
Sbjct: 92 SNDEA----LRNLNQIVNELDEEKYKD--------AYVYSIVAIARIKLISGKLDEAREL 139
Query: 148 LDAIGKTLDVQDSVPLRVTGAYYSCNASYYQVKKDFNNFYYTSLLYLSTITSXXXXXXXX 207
L K +D D V + +YYS +A YY+ K D+ +Y LLYLS I
Sbjct: 140 LVKASKIIDHIDYVESLIHSSYYSVSADYYKAKADYAQYYRHCLLYLSCID----LDKCS 195
Query: 208 XXXXXXXAYNLCIAALLGDNIYNFGELLQHPIISSISNDKEYEWLFRFLNALTEGDFQKF 267
A +L +AA+LGD IYNFGELL HP+ + ++EWL + A+ GD F
Sbjct: 196 HTELVERAVDLSVAAILGD-IYNFGELLLHPVFELLVGT-QHEWLHDLVIAMNVGDLPLF 253
Query: 268 DQISKERIPKVPILAQHESFLRQKICLMTLVESVFA--KSIRTLSFRDIADATFLPIDSV 325
+++ + I K+P+L + L QKI LM L+E VF + RTL+F IA AT +P + V
Sbjct: 254 ERLMGQ-INKMPLLQSSVALLGQKIRLMALIELVFQLPPNQRTLTFDTIARATRIPSNEV 312
Query: 326 EHLVMRSISLGLLKGSIDQVQQLVTITWVQPRIINQEQIKKMKDNLIKWQDEVSKLGNKI 385
E L+MR++S+GL+ G ID+V Q+VTI+ VQ RI+N QI M+ L +W + L N +
Sbjct: 313 ELLIMRALSVGLITGVIDEVTQIVTISSVQSRILNHSQIASMESRLREWNQNIKNLSNVV 372
Query: 386 EGRGKSIWV 394
E GK ++V
Sbjct: 373 EISGKGVFV 381
>7301037 [O] KOG2908 26S proteasome regulatory complex subunit RPN9/PSMD13
Length = 382
Score = 203 bits (517), Expect = 3e-52
Identities = 135/392 (34%), Positives = 204/392 (51%), Gaps = 24/392 (6%)
Query: 5 IDTILSTLKQEVNSELAPLFDDFEDLYERKLWHQLTEKLAIFF-HDS-KSESFRLRLYTQ 62
+ L+T K+ N ELA + E+LY KLW++LT KL F H+S + E+ L+LY
Sbjct: 8 VTAYLATQKKTTNKELAAEWTLIEELYNEKLWNELTIKLVTFVRHESLQDETALLQLYQN 67
Query: 63 FVSKFIDKINQLNVVEFLLQALQENDDTEESLQYLKDLQKEFKEIDEKKQRNDGLKPHVN 122
F+S F KIN +++ L + D +E++++L+ ++ + K DE ++
Sbjct: 68 FLSTFETKINPYGLIQILEVVVDNIGDKKEAIEFLEKMKDKVKICDEAVWYLQVMQ---- 123
Query: 123 GNLLLDIEIARVKLEKGDLVEVRDILDAIGKTLDVQDSVPLRVTGAYYSCNASYYQVKKD 182
GNL L + + K + E+RD+L+ G V G YY + YY+
Sbjct: 124 GNLYLS-NLNDLNATKKIIEELRDVLEEAGNVTPVH--------GKYYMLASQYYRRVGK 174
Query: 183 FNNFYYTSLLYLSTITSXXXXXXXXXXXXXXXAYNLCIAALLGDNIYNFGELLQHPIISS 242
+++Y L +L A+ L +AALLGD +YN GELL HPI+ S
Sbjct: 175 HSDYYRCGLQFLGC-----SMDEYPRDQWAQQAFFLGLAALLGDGVYNIGELLAHPILES 229
Query: 243 ISNDKEYEWLFRFLNALTEGDFQKFDQISKERIPKVPILAQHESFLRQKICLMTLVESVF 302
+ E EWL L A GD KF+ + K+ K+P LA E LRQKI L+ L+E F
Sbjct: 230 LKGT-ENEWLMELLKAFNTGDINKFNDM-KKIWSKIPDLAAQEVKLRQKISLLCLMEMTF 287
Query: 303 AKSI--RTLSFRDIADATFLPIDSVEHLVMRSISLGLLKGSIDQVQQLVTITWVQPRIIN 360
+S R +SF DIA T LP VE L+M++++L L++G IDQV +V ++WVQPR++N
Sbjct: 288 KRSAIERAISFTDIAQETKLPAKEVELLIMKALALDLVRGEIDQVAGVVNMSWVQPRVLN 347
Query: 361 QEQIKKMKDNLIKWQDEVSKLGNKIEGRGKSI 392
+ QI M L W ++ + +E R I
Sbjct: 348 RSQIVGMASTLDTWMGAITNMEKLMENRAAEI 379
>Hs4506223 [O] KOG2908 26S proteasome regulatory complex subunit RPN9/PSMD13
Length = 376
Score = 185 bits (470), Expect = 9e-47
Identities = 117/369 (31%), Positives = 199/369 (53%), Gaps = 24/369 (6%)
Query: 28 EDLYERKLWHQLTEKLAIFFHDS--KSESFRLRLYTQFVSKFIDKINQLNVVEFLLQALQ 85
E+LY +KLWHQLT ++ F D ++LY F+S+F ++N L++VE +L ++
Sbjct: 26 EELYTKKLWHQLTLQVLDFVQDPCFAQGDGLIKLYENFISEFEHRVNPLSLVEIILHVVR 85
Query: 86 ENDDTEESLQYLKDLQKEFKEIDEKKQRNDGLKPHVNGNLLLDIEIARVKLEKGDLVEVR 145
+ D +L +L+ +++ K DE +L I +KL GDL +
Sbjct: 86 QMTDPNVALTFLEKTREKVKSSDEAV-------------ILCKTAIGALKLNIGDLQVTK 132
Query: 146 DILDAIGKTLDVQDSVPLRVTGAYYSCNASYYQVKKDFNNFYYTSLLYLSTITSXXXXXX 205
+ ++ + + L+ V V +Y ++ YYQ + ++Y +L +L +
Sbjct: 133 ETIEDVEEMLNNLPGVT-SVHSRFYDLSSKYYQTIGNHASYYKDALRFLGCVD----IKD 187
Query: 206 XXXXXXXXXAYNLCIAALLGDNIYNFGELLQHPIISSISNDKEYEWLFRFLNALTEGDFQ 265
A+ L +A LLG+ ++NFGELL HP++ S+ N + +WL L A G+ +
Sbjct: 188 LPVSEQQERAFTLGLAGLLGEGVFNFGELLMHPVLESLRNT-DRQWLIDTLYAFNSGNVE 246
Query: 266 KFDQISKERIPKVPILAQHESFLRQKICLMTLVESVFAKSI--RTLSFRDIADATFLPID 323
+F Q K + P LA +E+ L +KI L+ L+E F + R L+F +IA + + ++
Sbjct: 247 RF-QTLKTAWGQQPDLAANEAQLLRKIQLLCLMEMTFTRPANHRQLTFEEIAKSAKITVN 305
Query: 324 SVEHLVMRSISLGLLKGSIDQVQQLVTITWVQPRIINQEQIKKMKDNLIKWQDEVSKLGN 383
VE LVM+++S+GL+KGSID+V + V +TWVQPR+++ +QIK MKD L W +V +
Sbjct: 306 EVELLVMKALSVGLVKGSIDEVDKRVHMTWVQPRVLDLQQIKGMKDRLEFWCTDVKSMEM 365
Query: 384 KIEGRGKSI 392
+E + I
Sbjct: 366 LVEHQAHDI 374
>Hs14774537 [O] KOG2908 26S proteasome regulatory complex subunit RPN9/PSMD13
Length = 376
Score = 185 bits (470), Expect = 9e-47
Identities = 117/369 (31%), Positives = 199/369 (53%), Gaps = 24/369 (6%)
Query: 28 EDLYERKLWHQLTEKLAIFFHDS--KSESFRLRLYTQFVSKFIDKINQLNVVEFLLQALQ 85
E+LY +KLWHQLT ++ F D ++LY F+S+F ++N L++VE +L ++
Sbjct: 26 EELYTKKLWHQLTLQVLDFVQDPCFAQGDGLIKLYENFISEFEHRVNPLSLVEIILHVVR 85
Query: 86 ENDDTEESLQYLKDLQKEFKEIDEKKQRNDGLKPHVNGNLLLDIEIARVKLEKGDLVEVR 145
+ D +L +L+ +++ K DE +L I +KL GDL +
Sbjct: 86 QMTDPNVALTFLEKTREKVKSSDEAV-------------ILCKTAIGALKLNIGDLQVTK 132
Query: 146 DILDAIGKTLDVQDSVPLRVTGAYYSCNASYYQVKKDFNNFYYTSLLYLSTITSXXXXXX 205
+ ++ + + L+ V V +Y ++ YYQ + ++Y +L +L +
Sbjct: 133 ETIEDVEEMLNNLPGVT-SVHSRFYDLSSKYYQTIGNHASYYKDALRFLGCVD----IKD 187
Query: 206 XXXXXXXXXAYNLCIAALLGDNIYNFGELLQHPIISSISNDKEYEWLFRFLNALTEGDFQ 265
A+ L +A LLG+ ++NFGELL HP++ S+ N + +WL L A G+ +
Sbjct: 188 LPVSEQQERAFTLGLAGLLGEGVFNFGELLMHPVLESLRNT-DRQWLIDTLYAFNSGNVE 246
Query: 266 KFDQISKERIPKVPILAQHESFLRQKICLMTLVESVFAKSI--RTLSFRDIADATFLPID 323
+F Q K + P LA +E+ L +KI L+ L+E F + R L+F +IA + + ++
Sbjct: 247 RF-QTLKTAWGQQPDLAANEAQLLRKIQLLCLMEMTFTRPANHRQLTFEEIAKSAKITVN 305
Query: 324 SVEHLVMRSISLGLLKGSIDQVQQLVTITWVQPRIINQEQIKKMKDNLIKWQDEVSKLGN 383
VE LVM+++S+GL+KGSID+V + V +TWVQPR+++ +QIK MKD L W +V +
Sbjct: 306 EVELLVMKALSVGLVKGSIDEVDKRVHMTWVQPRVLDLQQIKGMKDRLEFWCTDVKSMEM 365
Query: 384 KIEGRGKSI 392
+E + I
Sbjct: 366 LVEHQAHDI 374
>At5g45620 [O] KOG2908 26S proteasome regulatory complex subunit RPN9/PSMD13
Length = 375
Score = 160 bits (406), Expect = 2e-39
Identities = 103/334 (30%), Positives = 179/334 (52%), Gaps = 23/334 (6%)
Query: 9 LSTLKQEVNSELAPLFDDFEDLYERKLWHQLTEKLAIFFHDS--KSESFRLRLYTQFVSK 66
L +LK + EL ++ DLY++KLWHQLT KL F + ++ ++ Y F++
Sbjct: 7 LESLKN-THPELGEWYNSLADLYQKKLWHQLTLKLEQFIALAVFQAGDALIQFYHNFITD 65
Query: 67 FIDKINQLNVVEFLLQALQENDDTEESLQYLKDLQKEFKEIDEKKQRNDGLKPHVNGNLL 126
F KIN L + F + ++ + E ++ YL+ + ++ + E P + ++
Sbjct: 66 FETKINLLKLAHFAVVVSRQYSEKEAAVSYLESVIEKLRATKE---------PRITEPII 116
Query: 127 -LDIEIARVKLEKGDLVEVRDILDAIGKTLDVQDSVPLRVTGAYYSCNASYYQVKKDFNN 185
++ + A KLE+GD E + ILD +LD + V +Y ++ Y++ +++F++
Sbjct: 117 YIETQKALFKLEQGDQKECKKILDDGKSSLDSMTDIDPSVYANFYWVSSQYHKCRQEFSD 176
Query: 186 FYYTSLLYLSTITSXXXXXXXXXXXXXXXAYNLCIAALLGDNIYNFGELLQHPIISSISN 245
FY ++LLYL+ A++L ++ALLG+NIYNFGELL HPI+ S+
Sbjct: 177 FYKSALLYLA----YTSVEDLSESFKLDLAFDLSLSALLGENIYNFGELLAHPILKSLLG 232
Query: 246 DKEYEWLFRFLNALTEGD---FQKFDQISKERIPKVPILAQHESFLRQKICLMTLVESVF 302
EWL+ L A GD +Q+ ++ + P L ++E L +KI ++ L+E +F
Sbjct: 233 -TNVEWLYHILQAFNHGDLVQYQELCRVHNASLIAQPALVENEKKLLEKINILCLIEIIF 291
Query: 303 AKSI--RTLSFRDIADATFLPIDSVEHLVMRSIS 334
++ RT+ IA+ T L I+ VEHL+M+S+S
Sbjct: 292 SRPAEDRTIPLSIIAERTKLSIEDVEHLLMKSLS 325
>CE02329 [O] KOG2908 26S proteasome regulatory complex subunit RPN9/PSMD13
Length = 387
Score = 152 bits (383), Expect = 1e-36
Identities = 117/399 (29%), Positives = 200/399 (49%), Gaps = 34/399 (8%)
Query: 11 TLKQEVNSELA----PLFDDFE---DLYERKLWHQLTE------KLAIFFHDSKSESFRL 57
T + +N +LA PL DD++ +L+E+KLWHQLT K F + F
Sbjct: 2 TAQDYLNGKLAAANGPLADDWKNLKELWEKKLWHQLTVLTRSLVKKPQFVASTDMHEF-- 59
Query: 58 RLYTQFVSKFIDKINQLNVVEFLLQALQENDDTEESLQYLKDLQKEFKEIDEKKQRNDGL 117
Y FV+++ ++N L +VE + ++ +N T++ + ++ L K I++ K L
Sbjct: 60 --YRLFVAEWELRVNPLQLVEICI-SIAQNIATKDKQKSMEFLSKIGNVINKDKIAVARL 116
Query: 118 KPHVNGNLLLDIEIARVKLEKGDLVEVRDILDAIGKTLDVQDSVP--LRVTGAYYSCNAS 175
G + +E K + G +++++ I I T DS+ V +Y ++
Sbjct: 117 H---TGEIEARLEN---KDKNGQIIDLKSIRTQIDSTQHEVDSLVGVTEVHAPFYRVSSL 170
Query: 176 YYQVKKDFNNFYYTSLLYLSTITSXXXXXXXXXXXXXXXAYNLCIAALLGDNIYNFGELL 235
Y + DF +Y +L YL A L AALLG+N++NFGELL
Sbjct: 171 YLREVGDFAGYYREALRYLGV----EDANNLTTEQKQVHAVLLGFAALLGENVHNFGELL 226
Query: 236 QHPIISSISNDKEYEWLFRFLNALTEGDFQKFDQISKERIPKVPILAQHESFLRQKICLM 295
HPI+ S+ +E W+ L A GD +F + + L + + FL KI LM
Sbjct: 227 AHPILKSLEGTRE-RWIVDVLLAFNSGDLTRFFSLEGDW-GGWDDLKKQKDFLTAKIRLM 284
Query: 296 TLVESVFAK--SIRTLSFRDIADATFLPIDSVEHLVMRSISLGLLKGSIDQVQQLVTITW 353
++E ++ R++SF++IA +P D VE LVM+++S L++G I+QV+Q+V +TW
Sbjct: 285 AVMELAVSRPTKARSVSFKEIATKCQIPFDEVEFLVMKALSKDLIRGDINQVEQVVYVTW 344
Query: 354 VQPRIINQEQIKKMKDNLIKWQDEVSKLGNKIEGRGKSI 392
VQPR+++ QI +M + W+++V+ + + + I
Sbjct: 345 VQPRVLDNPQIMQMATRISAWRNDVNSMEGIVSKEAREI 383
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.138 0.390
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,492,296
Number of Sequences: 60738
Number of extensions: 873387
Number of successful extensions: 3445
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 3411
Number of HSP's gapped (non-prelim): 7
length of query: 394
length of database: 30,389,216
effective HSP length: 108
effective length of query: 286
effective length of database: 23,829,512
effective search space: 6815240432
effective search space used: 6815240432
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)