ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII0253 good MO KOG2459 Cell wall/membrane/envelope biogenesis GPI transamidase complex, GPI17/PIG-S component, involved in glycosylphosphatidylinositol anchor biosynthesis
r_klactII0253 good MO KOG2459 Posttranslational modification, protein turnover, chaperones GPI transamidase complex, GPI17/PIG-S component, involved in glycosylphosphatidylinositol anchor biosynthesis
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII0253 82972 84519 516
(516 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR434w [MO] KOG2459 GPI transamidase complex GPI17/PIG-S compon... 540 e-153
SPAC1F12.09 [MO] KOG2459 GPI transamidase complex GPI17/PIG-S co... 135 1e-31
Hs15088795 [MO] KOG2459 GPI transamidase complex GPI17/PIG-S com... 81 4e-15
At3g07180 [MO] KOG2459 GPI transamidase complex GPI17/PIG-S comp... 57 9e-08
7301234 [MO] KOG2459 GPI transamidase complex GPI17/PIG-S compon... 53 1e-06
>YDR434w [MO] KOG2459 GPI transamidase complex GPI17/PIG-S component
involved in glycosylphosphatidylinositol anchor
biosynthesis
Length = 534
Score = 540 bits (1392), Expect = e-153
Identities = 274/505 (54%), Positives = 360/505 (71%), Gaps = 19/505 (3%)
Query: 1 MSTVTLRRLVTLSFALCYVLLGVPLWYRLTTIYRAPLPAEYIESLHANLHEDIHLTIPVY 60
MS LR+ V F Y+ LGVPLWY+LTT+YRA LP YIESL N +DIHL IPVY
Sbjct: 1 MSNANLRKWVGFCFVAIYLFLGVPLWYKLTTVYRASLPINYIESLQNNKFQDIHLVIPVY 60
Query: 61 VQSDIYNFPDVCDAIQRQADELLQSADEHQRKVQWSLQILPFKEG----ETDPNNDHVVK 116
V+SD Y FPDV DAIQ Q + LL S ++ +V WSLQ+LP+ E E++ N HVV
Sbjct: 61 VKSDTYRFPDVHDAIQVQVNHLLNSQEQ---RVPWSLQVLPYNETIEQMESEGNQFHVVT 117
Query: 117 LVLDESMGLTIPQLTKETIIFFNDESLVNNDLPFFVAQALIEHTFKIEWEHFSKYHQNSV 176
L LDE +G + TKET+++++D ++++NDLPFFVAQ L+EHTF++EW H +K +
Sbjct: 118 LKLDEFIGYSSAYDTKETLVYYDDAAVLSNDLPFFVAQTLVEHTFQLEWTHLNK----TC 173
Query: 177 KSDSKSRNMAVNYDPNIHISISLLTGDAEPIAWDIDATLREYFTPIRDLLSPLVNFTVDT 236
+ S + ++A++YDPNIH+S++LL+GD P+AW+I+ TL +YF+P R LSPLVNFTVD+
Sbjct: 174 EGVSTNNDVAISYDPNIHLSVTLLSGDGNPVAWEIEPTLTDYFSPFRKFLSPLVNFTVDS 233
Query: 237 GIEYHNDLNLHSLSNA-EYVSWNDLSHTLDLSDLFSVNHYREQNSINLAIVFPSVETG-- 293
I YHNDLNLHSL+ + V+W DLSHT+DLS+L S+ +Y E +++NLAIVFPS +
Sbjct: 234 SIVYHNDLNLHSLNGSCTSVTWFDLSHTIDLSELSSMAYYPEDSALNLAIVFPSASSSPD 293
Query: 294 SLAFINETG-----NGIDWKSFLVPQWGVVVINKDPLPTNAYITNDYLADVISTFAHDIF 348
LAFIN T +DW S+LVPQWGV++INK PL N+ I+ DYL ++ FA DIF
Sbjct: 294 GLAFINGTRISDEITTLDWNSYLVPQWGVIIINKMPLKPNSVISEDYLEPMMYRFATDIF 353
Query: 349 KLLGLSEVAEDMNSPLVRIDSFKRKVIIDNLEKXXXXXXXXXXMTNHLQQMSIPREVLED 408
+LLGL+E ++D+ SP + IDSFKR I+ NL+K +T Q MSIPREV ++
Sbjct: 354 QLLGLTEGSQDLLSPYITIDSFKRLTILQNLDKATETLWSLVKLTQQFQGMSIPREVSDN 413
Query: 409 VNNALNLRLQIVDLLNNPELGSDEIWNEALRLSNRLVKICERAFFHKEMVQQTFFPQEHK 468
V AL+LRLQI+DLLN+P G D +WN AL LSN LVK+CE+AFF+ EMVQQ FFPQEH
Sbjct: 414 VIEALDLRLQIIDLLNDPGKGGDIVWNNALHLSNELVKLCEKAFFNGEMVQQNFFPQEHM 473
Query: 469 IAVYLPLLGPITVVTFTAFFKSLKE 493
IAVYLPLLGPI+ V F F+ +KE
Sbjct: 474 IAVYLPLLGPISAVMFFGFYNVMKE 498
>SPAC1F12.09 [MO] KOG2459 GPI transamidase complex GPI17/PIG-S component
involved in glycosylphosphatidylinositol anchor
biosynthesis
Length = 554
Score = 135 bits (341), Expect = 1e-31
Identities = 117/497 (23%), Positives = 219/497 (43%), Gaps = 33/497 (6%)
Query: 5 TLRRLVTLSFALCYVLLGVPLWYRLTTIYRAPLPAEYIESLHANLHEDIHLTIPVYVQSD 64
+L+R LSF + +LL +P+W++ T R+ LP E +E+ + + + + + D
Sbjct: 70 SLKRYALLSFYVI-ILLAIPVWWKTTHYERSSLPFEDMENAPSTVQTHLRFSPTFRILDD 128
Query: 65 IYNFPDVCDAIQRQADELLQSADEHQRKVQWSLQILPFKEGETDPNNDHVVKLVLDESMG 124
N + ++ ++L++ + ++L++L E DP + ++VL ES
Sbjct: 129 KGN------NLTKEVQKVLEAEPQIY---SYNLKVL-----EDDPVD---YRIVLRESTD 171
Query: 125 LTIPQLTKETIIFFNDESLVNNDLPFFVAQALIEHTFKIEWEHFSKYHQ--NSVKSDSKS 182
L E + S ++L + L E E +SK+ + ++V+
Sbjct: 172 LQ--WFWDENNFIIDTPSKGPSELAILIVNCLWEAFSPQVMEVWSKFTRFSSTVEPSRAE 229
Query: 183 RNMAVNYDPNIHISISLLTGDA--EPIAWDIDATLREYFTPIRDLLSPLVNFTVDTGIEY 240
V + P + +SLL G+ EPI WDI+ +++YF P+ + L+ L ++T I+Y
Sbjct: 230 TKRTVQFSPQYRVLLSLLVGEGNHEPINWDIENAIQKYFNPLIEQLASLAKLNIETQIQY 289
Query: 241 H-NDLNLHSLSNAEYVSWNDLSHTLDLSDLF-SVNHYREQNSINLAIVFPSVETGSLAFI 298
D + + DL + ++ + + S + + + +I+ + PS + L
Sbjct: 290 FVEDAEAYIKDDKFCTKHADLPNLVNNFEKYLSFSPHIREPTIHFVLYVPSPQIQPLWLE 349
Query: 299 NETGNGIDWKSFLVPQWGVVVINKDPLPTNAYITNDYLADVISTFAHDIFKLLGLSEVAE 358
NE N I S L+PQWG + + + + L D + D+ LLG+++V
Sbjct: 350 NEDSNIIPTNSMLLPQWGSITTINFNVTEKKLLHDVDLKDYFRVISRDLLLLLGINDVPV 409
Query: 359 DMNSPLVRIDSFKRKVIIDNLEKXXXXXXXXXXMTNHLQQMSIPREVLEDVNNALNLRLQ 418
S + D R+ I ++ + + + +Q M++P+E+ V + L
Sbjct: 410 SSLSATLA-DRLLRQRIAESCIEASDTLQNLAKLVHSMQSMAVPKEIQMYVKDTLLSLDM 468
Query: 419 IVDLLNNPELGSDEIWNEALRLSNRLVKICERAFFHKEMVQQTFFPQEHKIAVYLPLLGP 478
L+ L NEAL SN + A FH MV +FP E K +Y PL P
Sbjct: 469 AYKALSQNNL------NEALSYSNNAFSKSQEALFHPSMVTTIYFPDESKYGIYAPLFAP 522
Query: 479 ITVVTFTAFFKSLKEVI 495
I + +F K +K+++
Sbjct: 523 ILIPLLISFIKEVKDML 539
>Hs15088795 [MO] KOG2459 GPI transamidase complex GPI17/PIG-S component
involved in glycosylphosphatidylinositol anchor
biosynthesis
Length = 555
Score = 80.9 bits (198), Expect = 4e-15
Identities = 112/541 (20%), Positives = 204/541 (37%), Gaps = 77/541 (14%)
Query: 12 LSFALCYVLLGVPLWYRLTTIYRAPLPAEYIESLHANLHEDIHLTIPVYVQSDIYNFPDV 71
L FA ++LG+PLW++ T YRA LP I L+A + L +PV V
Sbjct: 21 LFFAAVAIVLGLPLWWKTTETYRASLPYSQISGLNAL---QLRLMVPVTVVF-------T 70
Query: 72 CDAIQRQADELLQSADEHQRKV--QWSLQI-----------LPFKEGETDPNNDHVVKLV 118
+++ E L H+R++ ++ ++I L +E + + +
Sbjct: 71 RESVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALDHEEEALSSGSVQEAEAM 130
Query: 119 LDESMGLTIPQLTKETIIFFNDESLVNNDLPFFVAQA--------LIEHTFKIEWEHFSK 170
LDE LT + SL+ D+ ++ + F I +
Sbjct: 131 LDEPQEQAEGSLT--VYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREAFNIIGRRIVQ 188
Query: 171 YHQN-SVKSD---------------SKSRNMAVNYDPNIHISISLLTGD--AEPIAWDID 212
Q S+ D S + + I+ SLL D + + WDI+
Sbjct: 189 VAQAMSLTEDVLAAALADHLPEDKWSAEKRRPLKSSLGYEITFSLLNPDPKSHDVYWDIE 248
Query: 213 ATLREYFTPIRDLLSPLVNFTVDTGIEYHNDLNLH----SLSNAEYVSWNDLSHTLD-LS 267
+R Y P + L NF+VD+ I Y+ L ++ S S++ Y+ + L H ++ +
Sbjct: 249 GAVRRYVQPFLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSSYYLDMHSLPHVINPVE 308
Query: 268 DLFSVNHYREQNSINLAIVFPSVETGSLAFINETGNGIDWKSFLVPQWG-VVVINKDPLP 326
+ +N + P + L ++ G + +F P+WG ++V N D
Sbjct: 309 SRLGSSAASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIMVYNVDSKT 368
Query: 327 TNAYI----TNDYLADVISTFAHDIFKLLGLSE--------VAEDMNSPLV--RIDSFKR 372
NA + + V+ F + L G+++ ++ + L+ +D
Sbjct: 369 YNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLW 428
Query: 373 KVIIDNLEKXXXXXXXXXXMTNHLQQMSIPREVLEDVNNALNLRLQIVDLLNNPELGSDE 432
++NL + + + I +V +V A+ + + L + L S
Sbjct: 429 ARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLAS-- 486
Query: 433 IWNEALRLSNRLVKICERAFFHKEMVQQTFFPQEHKIAVYLPLLGPITVVTFTAFFKSLK 492
A S V E AFF ++ +FP + K A+Y+PL P+ V + K
Sbjct: 487 ----AFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFL 542
Query: 493 E 493
E
Sbjct: 543 E 543
>At3g07180 [MO] KOG2459 GPI transamidase complex GPI17/PIG-S component
involved in glycosylphosphatidylinositol anchor
biosynthesis
Length = 605
Score = 56.6 bits (135), Expect = 9e-08
Identities = 69/319 (21%), Positives = 112/319 (34%), Gaps = 39/319 (12%)
Query: 185 MAVNYDPNIHISISLLTGDAEPIA--WDIDATLREYFTPIRDLLSPLVNFTVDTGIEYHN 242
M V D I +S SLL + WD P+ L+P+ N TV++ + YH
Sbjct: 273 MPVGSDGKIVLSFSLLNSNPRDWVYDWDFQRIDEALLAPVTKALAPIANITVESQVLYHT 332
Query: 243 DLNLHSLSNAEYVSWNDLSHTLDLSDLFSVNHYREQNS---------INLAIVFPSVETG 293
+ S + + S+ + T DL + N + S + + PS +
Sbjct: 333 PKSSFSSWDKKLQSY--IFRTSDLPFFVNSNEWHLDTSAGASGRSKILQFVVYIPSGKEC 390
Query: 294 SLAFINETGNGIDWKSFLVPQWGVVVI------NKDP-LPTNAYITNDYLADVISTFAHD 346
L F+ P WG V++ +KD P+ I+ L ++ F
Sbjct: 391 PLLLQLPNVEISKTNGFISPMWGGVIVWNPGNCDKDSESPSRNTISLQDLEQIVEIFLGQ 450
Query: 347 IFKLLGLSEVAE-------------DMNSPLVRIDSFKRKVIIDNLEKXXXXXXXXXXMT 393
+L G A+ + +D RK NL +
Sbjct: 451 FRQLFGFKSEAKYTTGLGTYKILTSERGFTEWELDVLSRKHTCFNLHSCSTTLGSLSRLV 510
Query: 394 NHLQQMSIPREVLEDVNNALNLRLQIVDLLNNPELGSDEIWNEALRLSNRLVKICERAFF 453
L +M I E+ E V +L +N LG ++ + S + E AFF
Sbjct: 511 RSLPRMIIKDEIGEQVKYSLKAAKLAQ---SNASLGG---YSSSASSSREARSLAENAFF 564
Query: 454 HKEMVQQTFFPQEHKIAVY 472
H ++ ++F EH AVY
Sbjct: 565 HPSIMSVSYFSYEHCFAVY 583
>7301234 [MO] KOG2459 GPI transamidase complex GPI17/PIG-S component
involved in glycosylphosphatidylinositol anchor
biosynthesis
Length = 347
Score = 53.1 bits (126), Expect = 1e-06
Identities = 65/310 (20%), Positives = 112/310 (35%), Gaps = 39/310 (12%)
Query: 209 WDIDATLREYFTPIRDLLSPLVNFTVDTGIEYHNDLNLH--------SLSNAEYVSWNDL 260
W+I ++ Y P +S + N+TV + +Y + L + L
Sbjct: 30 WNIAMAVKTYIEPWLAKVSGVSNYTVRSQWKYRVAIEADLKQVRDQSKLGRHYALQETAL 89
Query: 261 SHTLDLSDLFSVNHYREQNSINLAIVFPSVETGSLAFINE-----TGNGIDWKSFLVPQW 315
H L ++ +INL + P L N T N +D +F+ P W
Sbjct: 90 PHLLTSIAQNLSASTTDKPAINLVVYIPPCHIAPLHIYNSKDQRLTRNDVD--AFISPPW 147
Query: 316 GVVVINKDPLPTNAYITNDYLA--------DVISTFAHDIFKLLGLSEVAEDMNSPLVRI 367
G +I P +D A D + + KLL +S + +V I
Sbjct: 148 GGFIIANPPEHVCLAAMSDQEAVPYHVSTTDNMQVMLDQLHKLLDISSELQMEGVKVVDI 207
Query: 368 D----------SFKRKVIIDNLEKXXXXXXXXXXMTNHLQQMSIPREVLEDVNNALNLRL 417
+ ++ R+ I ++ + + + + I EV + N+
Sbjct: 208 EQLEPRRWEYEAYLRRSAIRHISTASSTLQSLIKLLDQISYIVIDDEVGAAITNSY---- 263
Query: 418 QIVDLLNNPELGSDEIWNEALRLSNRLVKICERAFFHKEMVQQTFFPQEHKIAVYLPLLG 477
D+L + +A L+ R ER FF ++ Q +FP E K A+Y+PL
Sbjct: 264 --ADILAAKAALLEHRLADASVLAKRAFVASERGFFDASLLAQLYFPDEQKYAIYIPLFL 321
Query: 478 PITVVTFTAF 487
PI V ++F
Sbjct: 322 PIMVPVLSSF 331
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.320 0.137 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,415,723
Number of Sequences: 60738
Number of extensions: 1286312
Number of successful extensions: 3015
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 3001
Number of HSP's gapped (non-prelim): 7
length of query: 516
length of database: 30,389,216
effective HSP length: 111
effective length of query: 405
effective length of database: 23,647,298
effective search space: 9577155690
effective search space used: 9577155690
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)