ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactII0286 good S KOG2765 Function unknown Predicted membrane protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactII0286 94268 95509 414
(414 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YML018c [S] KOG2765 Predicted membrane protein 335 6e-92
YDR438w [S] KOG2765 Predicted membrane protein 181 1e-45
SPBC405.03c [S] KOG2765 Predicted membrane protein 161 2e-39
Hs21361959 [S] KOG2765 Predicted membrane protein 96 1e-19
At4g32140 [S] KOG2765 Predicted membrane protein 82 1e-15
At3g07080 [S] KOG2765 Predicted membrane protein 75 2e-13
7303054 [S] KOG2765 Predicted membrane protein 59 1e-08
>YML018c [S] KOG2765 Predicted membrane protein
Length = 393
Score = 335 bits (860), Expect = 6e-92
Identities = 192/395 (48%), Positives = 235/395 (58%), Gaps = 24/395 (6%)
Query: 7 SQSKKWTXXXXXXXXXXXXXXXSSFLINMIFEDNSYRKPFFITYLNTASFIFYLVPTFKN 66
S +K+WT SSFLIN+IFED+SYRKPFFITY NTA+FIFYL PT K
Sbjct: 8 SFNKRWTLGLLMLGLVIILWVLSSFLINLIFEDDSYRKPFFITYTNTAAFIFYLFPTAKA 67
Query: 67 VWHKYKTTGKLNIHDELILEEEGQAIPNGTANNNTHVGXXXXXXXXXXXXXXEHSSLVSK 126
V YK TG+ N+H ELI+EEEG +++N V ++L +
Sbjct: 68 VVVNYKDTGRANVHRELIMEEEGTG-----SDSNRSVDMTSPLL----------TNLEAG 112
Query: 127 DSSDPTIKLPLRSTIKLSAQFCILWFLANLVTNASLSYTXXXXXXXXXXXXXXXXXXXXX 186
++ +L L TIKLSA+FCILWF ANLVTNASL++T
Sbjct: 113 THANQKKRLTLYETIKLSAEFCILWFTANLVTNASLAFTSVASQTILSTTSSFFTLFIGA 172
Query: 187 XCHVESVNKIKVLGSIVSFIGIIMVTKSDYSYVTASKIHFAIPYKFHNGIDYDNTSPVTX 246
CHVES++K KVLGS +SF+GIIMVTKSD S+ + H A + D+ V
Sbjct: 173 ICHVESLSKSKVLGSFISFVGIIMVTKSD-SHQRYQR-HIA-------DVSGDDNDAVQV 223
Query: 247 XXXXXXXXXXXXFYGVYSTLLKLKVQDESRINMKIXXXXXXXXXXXXXWPALILLHFTGK 306
YGVYSTLLK +V DE+R+NMKI WP+LI+L F G
Sbjct: 224 LIGNLLALAGAVLYGVYSTLLKREVGDETRVNMKIFFGFVGLFNLLFLWPSLIVLDFFGW 283
Query: 307 ETFEWPSSPRVLSIILTNCLITFISDFCWAKAMLLTSPLIVTVGLSTTIPLAMVGDFVFK 366
E F P P+V+ II NCLITF+SDFCWAKAMLLTSPL VTVGLS TIPLAM GD +FK
Sbjct: 284 EPFSLPKDPKVVVIIFVNCLITFVSDFCWAKAMLLTSPLTVTVGLSITIPLAMFGDVIFK 343
Query: 367 EKPMTALYLFGAVLICGSFFIVNRNAEEETINHVI 401
K M+ALYLFGA LI GSFFI+N+++EEE + I
Sbjct: 344 HKTMSALYLFGATLILGSFFIINKSSEEEHFENSI 378
>YDR438w [S] KOG2765 Predicted membrane protein
Length = 370
Score = 181 bits (460), Expect = 1e-45
Identities = 117/366 (31%), Positives = 174/366 (46%), Gaps = 41/366 (11%)
Query: 29 SSFLINMIFEDNSYRKPFFITYLNTASFIFYLVPTFKNVWHKYKTTGKLNIHDELILEEE 88
+S L N + E N+Y KPFF+TYLN +SF YL P ++W ++ K + E
Sbjct: 27 ASCLTNELLETNAYNKPFFLTYLNISSFALYLTP---DLWRIIQSRRK------SLQERT 77
Query: 89 GQAIPNGTANNNTHVGXXXXXXXXXXXXXXEHSSLVSKDSSDPTIKLPLRSTIKLSAQFC 148
+ +P H SS +S +D +K T++LS FC
Sbjct: 78 ERTLP-------IHTQESFSEFLPLLSSTPSTSSNLS-SIADTKVK----DTMRLSLLFC 125
Query: 149 ILWFLANLVTNASLSYTXXXXXXXXXXXXXXXXXXXXXXCHVESVNKIKVLGSIVSFIGI 208
+LWF+ANL NA+LSYT +E+ + K+LG VS GI
Sbjct: 126 VLWFVANLAANAALSYTTVASSTILSSTSSFFTLFLATSLGIETFSTKKLLGLFVSLFGI 185
Query: 209 IMVTKSDYSYVTASKIHFAIPYKFHNGIDYDNTSPVTXXXXXXXXXXXXXFYGVYSTLLK 268
I++ + +SK D+ S + Y VY+TLLK
Sbjct: 186 ILIV------MQSSK-------------QQDSVSASSFLVGNTLALLGSLGYSVYTTLLK 226
Query: 269 LKVQDES-RINMKIXXXXXXXXXXXXXWPALILLHFTGKETFEWPSSPRVLSIILTNCLI 327
++ + R+++++ WP LI+L T ETFE PS+ + +++ NC+I
Sbjct: 227 YEISSKGLRLDIQMFLGYVGIFTFLLFWPILIILDITHMETFELPSNFHISFLVMLNCII 286
Query: 328 TFISDFCWAKAMLLTSPLIVTVGLSTTIPLAMVGDFVFKEKPMTALYLFGAVLICGSFFI 387
F+SD+ W KA++LTSPL+VTV L+ TIPLAM DFV++E T Y+ G + I SFF+
Sbjct: 287 IFVSDYFWCKALILTSPLVVTVALTFTIPLAMFADFVWREAFFTPWYIIGVIFIFVSFFL 346
Query: 388 VNRNAE 393
VN E
Sbjct: 347 VNHRGE 352
>SPBC405.03c [S] KOG2765 Predicted membrane protein
Length = 341
Score = 161 bits (407), Expect = 2e-39
Identities = 103/365 (28%), Positives = 160/365 (43%), Gaps = 52/365 (14%)
Query: 29 SSFLINMIFEDNSYRKPFFITYLNTASFIFYLVPTFKNVWHKYKTTGKLNIHDELILEEE 88
SSFL + + +D+++ PF ITY+NT +F+FYL+P + K T K + EL + E
Sbjct: 26 SSFLTSSLLDDDNFFSPFLITYINTGTFVFYLIPWY----FSEKKTRKHRLMSELSMYE- 80
Query: 89 GQAIPNGTANNNTHVGXXXXXXXXXXXXXXEHSSLVSKDSSDPTIKLPLRSTIKLSAQFC 148
++ + + N T P L R T LS FC
Sbjct: 81 --SVHDSSFNLGTR----------------------------PNSPLGFRQTAYLSLGFC 110
Query: 149 ILWFLANLVTNASLSYTXXXXXXXXXXXXXXXXXXXXXXCHVESVNKIKVLGSIVSFIGI 208
I+WF AN +N+SL +T +VE K+L + S G+
Sbjct: 111 IIWFAANYFSNSSLGFTNVASFTIISSMSGFFTLGLGTIVNVERFTLSKLLALMASVGGV 170
Query: 209 IMVTKSDYSYVTASKIHFAIPYKFHNGIDYDNTSPVTXXXXXXXXXXXXXFYGVYSTLLK 268
I+V D D +++ P YG YS ++K
Sbjct: 171 IIVVTQDAKQA-----------------DLNDSPPSRPALGNAYALLAALLYGCYSVMVK 213
Query: 269 LKVQDESRINMKIXXXXXXXXXXXXXWPALILLHFTGKETFEWPSSPRVLSIILTNCLIT 328
+ +ES ++ ++ WP LI+LH G E F PS+ L +++ N IT
Sbjct: 214 FHITEESCVSTRLFFGLVGLFDLILLWPFLIILHLYGVERFSLPSTTAGLIVLIINASIT 273
Query: 329 FISDFCWAKAMLLTSPLIVTVGLSTTIPLAMVGDFVFKEKPMTALYLFGAVLICGSFFIV 388
F+SD+ W AML+TSPL+VTVG+S +IPLA+ D + K + + G++L+ F +V
Sbjct: 274 FVSDYLWVIAMLMTSPLLVTVGMSLSIPLALFFDILLKGHYLNFSLILGSLLVFAGFIVV 333
Query: 389 NRNAE 393
N N +
Sbjct: 334 NYNQQ 338
>Hs21361959 [S] KOG2765 Predicted membrane protein
Length = 523
Score = 95.9 bits (237), Expect = 1e-19
Identities = 70/258 (27%), Positives = 109/258 (42%), Gaps = 26/258 (10%)
Query: 134 KLPLRSTIKLSAQFCILWFLANLVTNASLSYTXXXXXXXXXXXXXXXXXXXXXXCHVESV 193
KL K+S FC +WFLANL +LS T S
Sbjct: 234 KLTATQVAKISFFFCFVWFLANLSYQEALSDTQVAIVNILSSTSGLFTLILAAVFPSNSG 293
Query: 194 NKI---KVLGSIVSFIGIIMVTKSDYSYVTASKIHFAIPYKFHNGIDYDNTSPVTXXXXX 250
++ K+L I+S G+++V + S A + D +
Sbjct: 294 DRFTLSKLLAVILSIGGVVLVNLAG-SEKPAGR---------------DTVGSIWSLAGA 337
Query: 251 XXXXXXXXFYGVYSTLLKLKVQDESRINMKIXXXXXXXXXXXXXWPALILLHFTGKETFE 310
Y VY ++K KV E ++++ + WP LLH+TG E FE
Sbjct: 338 M-------LYAVYIVMIKRKVDREDKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFEDFE 390
Query: 311 WPSSPRVLSIILTNCLITFISDFCWAKAMLLTSPLIVTVGLSTTIPLAMVGDFVFKEKPM 370
+P+ ++ II+ + T +S+F W LTS LI T+ LS TIPL+++ D ++
Sbjct: 391 FPNKVVLMCIIINGLIGTVLSEFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQF 450
Query: 371 TALYLFGAVLICGSFFIV 388
+ L+ GA+ + SFFIV
Sbjct: 451 SWLFFAGAIPVFFSFFIV 468
>At4g32140 [S] KOG2765 Predicted membrane protein
Length = 394
Score = 82.4 bits (202), Expect = 1e-15
Identities = 79/366 (21%), Positives = 146/366 (39%), Gaps = 31/366 (8%)
Query: 41 SYRKPFFITYLNTASFIFYLVPTFKNVWHKYKTTGKLNIHDELILEEEGQAIPNGTANNN 100
+Y++PF +TYL + I YL F W + + +++ + ++ G+ +
Sbjct: 32 AYKQPFAVTYLGASLMIVYLPVAFLKDWLCRYLDRRSSKSNKIPALTDDSSVELGSPLRH 91
Query: 101 THVGXXXXXXXXXXXXXXEHSSLVSKDSSDP----------TIKLPLRSTIKLSAQFCI- 149
+ EH S ++ P T+K T K A + +
Sbjct: 92 KII-EMGLQGTITKKDSEEHLSSHEEEDERPLIGKVREEVQTLKQRKEITTKQIAMYGLY 150
Query: 150 ---LWFLANLVTNASLSYTXXXXXXXXXXXXXXXXXXXXXXCHVESVNKIKVLGSIVSFI 206
+WF+ ++NA+L+ T +++N KV+ VS
Sbjct: 151 LAPIWFVTEYLSNAALARTSVASTTVLSSTSGLFTLFIGVFLGQDTLNLSKVVAVFVSMA 210
Query: 207 GIIMVTKSDYSYVTASKIHFAIPYKFHNGIDYDNTSPVTXXXXXXXXXXXXXFYGVYSTL 266
G++M T S+++ ++ NG YG+++ L
Sbjct: 211 GVVMTTLGKTWASDESQLNSSL-----NG--------ERSLMGDLFGLLSAVSYGLFTVL 257
Query: 267 LKLKVQDESR-INMKIXXXXXXXXXXXXXWPALILLHFTGKET-FEWPSSPRVLSIILTN 324
LK +E ++++ W + L G E F P S +V ++L N
Sbjct: 258 LKKFAGEEGEGVDVQKLFGYIGLFTLVALWWLVWPLTALGIEPKFTIPHSVKVDEVVLAN 317
Query: 325 CLI-TFISDFCWAKAMLLTSPLIVTVGLSTTIPLAMVGDFVFKEKPMTALYLFGAVLICG 383
I + +SD+ WA +++ T+PL+ T+G+S TIPLAMV D + + +A+Y+ G+ +
Sbjct: 318 GFIGSVLSDYFWALSVVWTTPLVATLGMSLTIPLAMVADMMIHGRHYSAIYILGSTQVFA 377
Query: 384 SFFIVN 389
F I N
Sbjct: 378 GFVIAN 383
>At3g07080 [S] KOG2765 Predicted membrane protein
Length = 438
Score = 75.1 bits (183), Expect = 2e-13
Identities = 70/303 (23%), Positives = 114/303 (37%), Gaps = 31/303 (10%)
Query: 85 LEEEGQAIPNGTANNNTHVGXXXXXXXXXXXXXXEHSSLVSKDSSDPTIKLPLRSTIKLS 144
+ E G I +G N + + +S VS D + K+S
Sbjct: 116 ISEGGNGIESGLENIELEIDASVNVS--------DGASGVSNKGLDEKGRWTRMRVAKVS 167
Query: 145 AQFCILWFLANLVTNASLSYTXXXXXXXXXXXXXXXXXXXXXXCHVESVNKIKVLGSIVS 204
C WFLA L N SL YT E +K+ ++
Sbjct: 168 LVICPFWFLAQLTFNVSLKYTTVTSNTILSSASSLFTFLVSLIFLGEKFTWLKLFSVLLC 227
Query: 205 FIGIIMVTKSDYSYVTASKIHFAIPYKFHNGIDYDNTSPVTXXXXXXXXXXXXXFYGVYS 264
G I+V+ D + SK + N + D S V+ Y VY
Sbjct: 228 MSGTIIVSMGD----SESKSNAVAK----NPLLGDILSLVSAA-----------LYAVYI 268
Query: 265 TLLKLKVQDESRINMKIXXXXXXXXXXXXXW----PALILLHFTGKETFEWPSSPRVLSI 320
TL++ K+ D+ N ++ + P ++L+FT +E F + + +
Sbjct: 269 TLIRKKLPDDDERNGRVSMAQFLGFLGLFNFFIFLPPALILNFTKRERFNALTLKQFGLV 328
Query: 321 ILTNCLITFISDFCWAKAMLLTSPLIVTVGLSTTIPLAMVGDFVFKEKPMTALYLFGAVL 380
+ L +SD+ WAKA+LLT+ + T GL+ +PLA + D + KP Y+ A +
Sbjct: 329 VGKGLLDNVLSDYLWAKAVLLTTTTVATAGLTIQVPLAAIVDSLSGNKPSFTDYIGAAAV 388
Query: 381 ICG 383
+ G
Sbjct: 389 MVG 391
>7303054 [S] KOG2765 Predicted membrane protein
Length = 426
Score = 59.3 bits (142), Expect = 1e-08
Identities = 77/399 (19%), Positives = 137/399 (34%), Gaps = 98/399 (24%)
Query: 29 SSFLINMIFEDNSYRKPFFITYLNTASFIFYLVPTFKNVWHKYKTTGKLNIHDELILEEE 88
SS L ++ + ++ KPFF TY T+ F YL+ + +K + + + ++E+
Sbjct: 25 SSELTKFLYNEANFDKPFFCTYFKTSMFSIYLLVI--GILAPWKESCERQNGNYAMMEQN 82
Query: 89 G--------QAI-------------------PNGTANNNTHVGXXXXXXXXXXXXXXEHS 121
QA+ NGT+N+ + G + +
Sbjct: 83 ADDENYYSNQAVLGDPTYVPIRSPHLGAGGQSNGTSNSIS--GTESDDSSVRSVRFSKMA 140
Query: 122 SLVSKDSSDPTIKLPLR----------------STIKLSAQFCILWFLANLVTNASLSYT 165
+ + + T L R T K + FC+LWF AN +L
Sbjct: 141 EVREMSAHEATDALMARLSYAASLRIRRQKTHHKTAKTALLFCLLWFAANYFFQLALEMD 200
Query: 166 XXXXXXXXXXXXXXXXXXXXXXCHVESVNKI---KVLGSIVSFIGIIMVTKSDYSYVTAS 222
+ +K+ KV+ ++ G++ +T +D
Sbjct: 201 EAAMITLVSSTSSFFIICLAAVFPSATGDKLTITKVIAVAMNIGGVVAITMND------- 253
Query: 223 KIHFAIPYKFHNGIDYDNTSPVTXXXXXXXXXXXXXFYGVYSTLLKLKVQDESRINMKIX 282
+H K G+ FY Y +K K E ++++ +
Sbjct: 254 -LHDT---KMTRGV--------------LLALFSAFFYAAYLVFVKRKSDTEEKVDIPLF 295
Query: 283 XXXXXXXXXXXXWPALILLHFTGKETFEWPSSPRVLSIILTNCLITFISDFCWAKAMLLT 342
ETFE PS + + L + T +S+ W LT
Sbjct: 296 F-----------------------ETFELPSQGQFALLFLNGLVGTVLSEALWLWGCFLT 332
Query: 343 SPLIVTVGLSTTIPLAMVGDFVFKEKPMTALYLFGAVLI 381
S LI T+ +S IPLA++ D + K KP + ++ G++ I
Sbjct: 333 SSLIGTLAMSLQIPLAILFDVLLKNKPYSPMFYMGSIPI 371
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.322 0.136 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,977,527
Number of Sequences: 60738
Number of extensions: 652199
Number of successful extensions: 1200
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1178
Number of HSP's gapped (non-prelim): 16
length of query: 414
length of database: 30,389,216
effective HSP length: 109
effective length of query: 305
effective length of database: 23,768,774
effective search space: 7249476070
effective search space used: 7249476070
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)